Abstract:
Introduction:Multiple myeloma (MM) remains an incurable disease, and patient survival requires a better understanding of this malignancy's molecular aspects. Heparanase (HPSE) is highly expressed in aggressive MM cells and related to tumor growth, metastasis, and bortezomib (BTZ) resistance. Thus, targeting HPSE seems to be a promising approach for MM treatment, and because microRNAs (miRNAs) have emerged as potential regulators of HPSE expression, the use of extracellular vesicles (EVs) can allow the efficient delivery of therapeutic miRNAs. Methods:We used prediction algorithms to identify potential miRNAs that regulate negatively HPSE expression. RT-qPCR was performed to assess miRNAs and HPSE expression in MM lines (U266 and RPMI-8226). Synthetic miRNA mimics were electroporated in MM cells to understand the miRNA contribution in HPSE expression, glycosaminoglycans (GAGs) profile, cell proliferation, and cell death induced by BTZ. EVs derived from HEK293T cells were engineered with miRNAs to evaluate their therapeutic potential combined with BTZ. Results:It revealed a direct association between BTZ sensitivity, HPSE, and miR-1252-5p expressions. Moreover, overexpression of miR-1252-5p significantly reduced HPSE expression and HPSE enzymatic activity in MM cells. The higher level of miR-1252-5p was correlated with a reduction of cell viability and higher sensitivity to BTZ. Further, EVs carrying miR-1252-5p increased MM cells' sensitivity to BTZ treatment. Conclusion:These results showed that miR-1252-5p could negatively regulate HPSE in MM, indicating the use of EVs carrying miR-1252-5p as a potential novel BTZ sensitization approach in MM cells.
journal_name
Onco Targets Therjournal_title
OncoTargets and therapyauthors
Rodrigues-Junior DM,Pelarin MFA,Nader HB,Vettore AL,Pinhal MASdoi
10.2147/OTT.S286751subject
Has Abstractpub_date
2021-01-15 00:00:00pages
455-467issn
1178-6930pii
286751journal_volume
14pub_type
杂志文章abstract:Background:Previous reports show that SIRT6 serves as a critical modulator of the development of multiple malignancies as well as other disorders. However, its role in nasopharyngeal carcinoma (NPC) is unknown. Thus, we elucidated the effects of SIRT6 on the survival of NPC cells, and modulation of cell death. Methods...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S179866
更新日期:2018-11-01 00:00:00
abstract:Background:Nasopharyngeal carcinoma (NPC) is a common malignant cancer that is distributed particularly in Southeastern Asia. Previous studies have manifested that long noncoding RNA urothelial carcinoma associated 1 (UCA1) was involved in NPC tumorigenesis and metastasis. However, the biological mechanism of UCA1 for ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S215819
更新日期:2019-10-11 00:00:00
abstract:Aim:To explore the expression and clinical significance of long noncoding RNA (lncRNA) gastric cancer-associated transcript 3 (GACAT3) in human colorectal cancer (CRC). Methods:Expression of GACAT3 in CRC tissues and cell lines was measured using quantitative real-time PCR. CCK-8 and colony formation assays were used ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S144103
更新日期:2018-03-19 00:00:00
abstract::N6-methyladenosine (m6A) demethylase fat mass and obesity-associated gene(FTO), previously recognized to be related with obesity and diabetes, was gradually discovered to be dysregulated in multiple cancers and plays an oncogenic or tumor-suppressive role. However, the specific expression and pro- or anti-cancer role ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S283417
更新日期:2020-12-15 00:00:00
abstract:BACKGROUND:Experimental disruption of the telomere overhang induces a potent DNA damage response and is the target of newly emerging cancer therapeutics. Introduction of T-oligo, an eleven-base oligonucleotide homologous to the 3'-telomeric overhang, mimics telomere disruption and induces DNA damage responses through a...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S54575
更新日期:2013-12-18 00:00:00
abstract:Introduction:Melanoma has been reported as the most common malignancy in skin cancer. The small nucleolar RNA host gene 5 (SNHG5), an lncRNA, has been proven as a vital regulator in several types of carcinoma. This study was designed to investigate the detailed roles and possible mechanisms of SNHG5 in melanoma progres...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S184078
更新日期:2018-12-24 00:00:00
abstract:Background:Pulmonary carcinosarcomas (PCSs) are a heterogeneous group of non-small-cell lung carcinomas (NSCLCs) with aggressiveness and a poor prognosis. Although genetic mutations of some common lung cancer subtypes have been extensively studied, the molecular characteristics of PCSs and the existence of abnormal tar...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S264617
更新日期:2020-08-10 00:00:00
abstract::[This corrects the article DOI: 10.2147/OTT.S214060.]. ...
journal_title:OncoTargets and therapy
pub_type: 已发布勘误
doi:10.2147/OTT.S258499
更新日期:2020-05-26 00:00:00
abstract::We report the case of a man with an advanced nonkeratinizing squamous cell thymic carcinoma harboring c-KIT exon 13 missense mutation K642E. This aberration is rare and has never been described previously in patients with thymic cancers. It has been found in a small number of cases of gastrointestinal stromal tumor an...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S58902
更新日期:2014-05-08 00:00:00
abstract:Background:Triple-negative breast cancer has become an intricate part and hotspot in the clinical and experimental research. Connexins, serving as functional proteins in gap junctions, play an important role in tumorigenesis, cell proliferation and metastasis. Methods:We constructed and employed the Connexin 43 (Cx43)...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S229076
更新日期:2020-06-10 00:00:00
abstract:Purpose:Paclitaxel resistance in ovarian cancer has become an urgent clinical problem. This study investigated the regulatory effects of RAB17 on the non-coding RNA network of the paclitaxel-resistant ovarian cancer cell A2780/PTX. Methods:Microarray analysis was used to identify differentially expressed genes in pacl...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S285153
更新日期:2020-12-24 00:00:00
abstract:Objective:Long non-coding RNA (lncRNA) ANRIL is emerging as a crucial role in ovarian cancer progression and prognosis. However, the precise molecular mechanism of ANRIL on ovarian cancer is not known. Thus, we aim to study the underlying mechanism of ANRIL on the action. Methods:The MTT assay assessed cell viability....
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S273614
更新日期:2021-01-19 00:00:00
abstract::Immune checkpoint inhibitors (ICIs) have transformed the therapeutic strategy and prognosis of advanced non-small cell lung cancer (NSCLC) patients. Nowadays, ICIs as monotherapy or in combination with chemotherapy are the standard of care treatment in advanced NSCLC, and in stage III, durvalumab (a programmed death l...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S259308
更新日期:2020-07-14 00:00:00
abstract:Background:Bromodomain and extra-terminal domain inhibitors like JQ1 have proved to be promising epigenetic agents for the treatment of malignant ovarian carcinoma. However, the resistance of ovarian cancer cells to BET inhibitors has not been elucidated. In this study, we investigated the potential mechanisms underlyi...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S220267
更新日期:2019-10-03 00:00:00
abstract:Background:Breast cancer resistance protein BCRP, belonging to superfamily G of the adenosine triphosphate-binding cassette (ABC) transporters, is an efflux pump and plays a critical role in protecting cells against xenobiotics and toxic compounds including (pro)carcinogens. BCRP is expressed in many tissues, including...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S195245
更新日期:2019-02-27 00:00:00
abstract:Introduction:The consolidation of different therapies into a single nanoplatform has shown great promise for synergistic tumor treatment. In this study, a multifunctional platform by WS2 quantum dots (WQDs)-coated doxorubicin (DOX)-loaded periodic mesoporous organosilicas (PMOs-DOX@WQDs) nanoparticles were fabricated f...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S160748
更新日期:2018-04-05 00:00:00
abstract::This report describes a patient with intravascular large B-cell lymphoma (IVLBCL) with central nervous system involvement at the time of diagnosis who achieved complete remission for over 5 years in response to therapy. The patient, a 71 year-old woman, was previously healthy with the exception of taking verapamil for...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S72596
更新日期:2014-11-18 00:00:00
abstract::Survivin is a member of the inhibitor-of-apoptosis proteins (IAPs) family; its overexpression has been widely demonstrated to occur in various types of cancer. Overexpression of survivin also correlates with tumor progression and induces anticancer drug resistance. Interestingly, recent studies reveal that survivin ex...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S33374
更新日期:2013-10-16 00:00:00
abstract:Background:Acute myeloid leukemia (AML) is the most common form of acute leukemia in adults. Long non-coding RNA taurine-upregulated gene 1 (lncRNA TUG1) has been discovered to participate in multiple cancers including AML. However, the detailed mechanism of TUG1 in AML remains obscure. Materials and methods:AML cell ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S217795
更新日期:2019-11-29 00:00:00
abstract::Basal cell carcinoma (BCC) is a common malignant tumor throughout the world. One of the known risk factors of BCC is intense exposure to ultraviolet radiation. More than 50% of BCCs of the eyelid initially occur on the lower lid. The gold standard of diagnosis of BCC is histopathology. Treatment options for BCC consis...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S130371
更新日期:2017-05-08 00:00:00
abstract:BACKGROUND:To evaluate the benefit of second-line chemotherapy with platinum-based treatment in patients with recurrent small cell lung cancer (SCLC). PATIENTS AND METHODS:A total of 535 patients continued with follow-up or best supportive care if needed, and 229 patients who progressed after the completion of first-l...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S52330
更新日期:2013-10-22 00:00:00
abstract:Background:Hepatocellular carcinoma (HCC) is the leading cause of cancer-related death worldwide. LINC00460, a novel long non-coding RNA (lncRNA), was recently confirmed as an oncogene in various cancers. However, the biological function and underlying mechanism of LINC00460 in HCC is largely obscure. Methods:Fifty pa...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S239258
更新日期:2020-03-06 00:00:00
abstract:Background:Cordycepin, the main active ingredient of a traditional Chinese herbal remedy - extracted from Cordyceps sinensis - has been demonstrated as a very effective anti-inflammatory and antitumor drug. The present study investigated its antitumor effect on pancreatic cancer, a highly aggressive cancer with extreme...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S164670
更新日期:2018-08-01 00:00:00
abstract::Circulating tumor cells (CTCs) possess profound influence on tumor metastases and disease progression. This study aimed to investigate the correlation of CTCs with clinical characteristics and T-cell immunity, and to explore whether CTCs and the subpopulations can serve as an independent prognostic factor in advanced ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S130087
更新日期:2017-05-04 00:00:00
abstract::Patients with Lynch Syndrome (LS) are at high risk of developing colorectal cancer at an early age. Germline mutations in DNA mismatch repair genes and microsatellite instability are clear signatures of this autosomal dominant disorder. Here, we report the clinical history of a 38-year-old patient with LS-related meta...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S167645
更新日期:2018-10-23 00:00:00
abstract:Purpose:Non-small cell lung cancer (NSCLC) patients with EGFR mutation are suffering from a high incidence of brain metastasis (BM). It is still controversial whether cranial radiotherapy could be delayed when the EGFR-tyrosine kinase inhibitors (TKIs) used as first-line therapy for EGFR-positive patients with BM. This...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S223216
更新日期:2019-10-08 00:00:00
abstract::Human testis development-related gene 1 (TDRG1) is a recently identified gene that is expressed exclusively in the testes and promotes the development of testicular germ cell tumors. In this study, the role of TDRG1 in the development of testicular seminoma, which is the most common testicular germ cell tumor, was fur...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S97294
更新日期:2016-01-20 00:00:00
abstract::The incidence of lung neuroendocrine carcinomas, which originate from lung neuroendocrine cells, is 1.35/100,000, among which mixed neuroendocrine carcinomas are very rare. Because of the heterogeneity and significant differences in sensitivity to treatments, there is no effective treatment, and the prognosis is poor....
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S210699
更新日期:2019-11-14 00:00:00
abstract:Background:It was previously reported that downregulation of miR-218 promoted thyroid cancer cell invasion, migration, and proliferation. However, the biological functions of miR-218 and its possible regulatory mechanisms in papillary thyroid cancer (PTC) cells are still elusive. Materials and methods:The expression l...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S172152
更新日期:2018-09-28 00:00:00
abstract:BACKGROUND:Current cancer treatments have unexpected side effects of which the death of normal cells is one. In some cancers, iron nanoparticles (NPs) can be subjected to diagnosis and passive targeting treatment. Cold atmospheric plasma (CAP) has a proven induction of selective cell death ability. In this study, we ha...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S95644
更新日期:2016-09-28 00:00:00