Abstract:
Objective:Long non-coding RNA (lncRNA) ANRIL is emerging as a crucial role in ovarian cancer progression and prognosis. However, the precise molecular mechanism of ANRIL on ovarian cancer is not known. Thus, we aim to study the underlying mechanism of ANRIL on the action. Methods:The MTT assay assessed cell viability. Cell migration and invasion were determined using the wound healing assay, Transwell migration, and invasion assay. The relationships of ANRIL, miR-324-5p, and RAN were evaluated using luciferase activity assay and RNA pull-down assay. Cancer stem cell was identified by flow cytometry. Sphere formation assay was conducted to determine the stem-like properties. Xenograft tumor was established to assess tumor growth in vivo. qRT-PCR and Western blot were used to detect gene expression. Results:ANRIL was elevated while miR-324-5p was decreased in ovarian cancer tissues and cells. Besides, downregulated ANRIL enhanced miR-324-5p expression, and the luciferase reporting experiment and RNA pull-down assay showed the binding interaction between ANRIL and miR-324-5p. miR-324-5p directly targeted Ran and negatively modulated the expression of Ran. Besides, Ran was promoted by overexpressed ANRIL, which was reversed by overexpression of miR-324-5p. Furthermore, decreased ANRIL and increased miR-324-5p suppressed tumor growth, migration capacity, drug resistance, and alleviated stem-like characteristics in vitro and in vivo. Ran mediated the regulation of ANRIL on cell viability, stem-like properties, and drug resistance of ovarian cancer cells. Conclusion:The ANRIL/miR-324-5p/Ran axis regulated ovarian cancer development, making the axis meaningful targets for ovarian cancer therapy.
journal_name
Onco Targets Therjournal_title
OncoTargets and therapyauthors
Wang K,Hu YB,Zhao Y,Ye Cdoi
10.2147/OTT.S273614subject
Has Abstractpub_date
2021-01-19 00:00:00pages
565-576issn
1178-6930pii
273614journal_volume
14pub_type
杂志文章abstract:AIMS:Metastasis-associated gene 2 (MTA2) is reported to play an important role in tumor progression, but little is known about the role of MTA2 in nasopharyngeal carcinoma (NPC). The aim of the study was to explore the expression and function of MTA2 in NPC. METHODS:Expression of MTA2 in NPC tissues and cell lines was...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S96518
更新日期:2016-03-18 00:00:00
abstract:Purpose:Bulky non-small cell lung cancer (NSCLC) is difficult to achieve effective local control by conventionally fractionated radiotherapy (CRT). The present work aims to evaluate the safety and efficacy of partial stereotactic ablative boost radiotherapy (P-SABR) in bulky NSCLC. Patients and methods:From December 2...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S159538
更新日期:2018-05-08 00:00:00
abstract:OBJECTIVE:To study microRNA (miR)-20a expression in hepatocellular carcinoma (HCC) and its effects on the proliferation, migration, and invasion of HepG2. METHODS:The real-time polymerase chain reaction was used to detect the expression of miR-20a in HCC tissue and normal tissue, as well as in HCC cell lines and norma...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S96861
更新日期:2016-05-27 00:00:00
abstract::Intrahepatic cholangiocarcinomas (IHCCs) are morphologically and biologically similar to pancreatic ductal adenocarcinomas (PDACs), so newly identified PDAC-associated genes or proteins could provide clues for screening novel biomarkers for IHCC. In this study, the expression of three novel PDAC tumor markers (T-box t...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S39646
更新日期:2013-01-01 00:00:00
abstract:PURPOSE:We validated the use of expression sequence tags (ESTs) as an effective method of screening for DNA segments that could predict urothelial cell carcinoma and for identifying ESTs with such predictive value. PATIENTS AND METHODS:From 2004 to 2009, eleven patients were enrolled in this study: six with high-grade...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S69239
更新日期:2014-09-30 00:00:00
abstract::We report the case of a man with an advanced nonkeratinizing squamous cell thymic carcinoma harboring c-KIT exon 13 missense mutation K642E. This aberration is rare and has never been described previously in patients with thymic cancers. It has been found in a small number of cases of gastrointestinal stromal tumor an...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S58902
更新日期:2014-05-08 00:00:00
abstract::The study aimed to investigate the difference of setup errors on different registration in the treatment of nasopharyngeal carcinoma based on weekly cone-beam computed tomography (CBCT). Thirty nasopharyngeal cancer patients scheduled to undergo intensity-modulated radiotherapy (IMRT) were prospectively enrolled in th...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S87159
更新日期:2015-09-14 00:00:00
abstract:Background:Malignant glioma is the second leading cause of cancer-related death worldwide, and is known to exhibit a high degree of heterogeneity in its deregulation of different oncogenic pathways. The molecular subclasses of human glioma are not well known. Thus, it is crucial to identify vital oncogenic pathways in ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S139243
更新日期:2017-11-15 00:00:00
abstract:Purpose:Traditional Chinese medicine (TCM) has gained increasing attention for the treatment of multiple chronic diseases, such as cancer. Here we aim to identify the antitumor activity of Sichong formula, a novel TCM, in human gastric cancer cells and investigate the underlying mechanisms. Methods:The AGS and MKN45 g...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S199605
更新日期:2019-07-16 00:00:00
abstract:OBJECTIVES:Long non-coding RNA (lncRNA) associated with microvascular invasion in hepatocellular carcinoma (MVIH) has been recently reported to act as a predictor for the poor recurrence-free survival of hepatocellular carcinoma (HCC) after hepatectomy. However, the biological role of MVIH in the tumorigenesis of HCC i...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S86807
更新日期:2015-08-27 00:00:00
abstract:BACKGROUND:CD155, an immunoglobulin-like adhesion molecule, plays an important role in carcinoma such as cells migration, proliferation, metastasis, and tumor immune. The upregulation of CD155 has been found in several human malignancies, but its expression in cholangiocarcinoma (CCA) still remains unclear. The aim of ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S141476
更新日期:2017-07-31 00:00:00
abstract::Immune checkpoint inhibitors (ICIs) have transformed the therapeutic strategy and prognosis of advanced non-small cell lung cancer (NSCLC) patients. Nowadays, ICIs as monotherapy or in combination with chemotherapy are the standard of care treatment in advanced NSCLC, and in stage III, durvalumab (a programmed death l...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S259308
更新日期:2020-07-14 00:00:00
abstract:BACKGROUND:The PI3K family participates in multiple signaling pathways to regulate cellular functions. PI3K/Akt signaling pathway plays an important role in tumorigenesis and development. O-GlcNAcylation, a posttranslational modification, is thought to modulate a wide range of biological processes, such as transcriptio...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S82845
更新日期:2015-11-09 00:00:00
abstract::Colorectal cancer continues to be an important public health concern, despite improvements in screening and better systemic chemotherapy. The integration of targeted therapies in the treatment of colon cancer has resulted in significant improvements in efficacy outcomes. Angiogenesis is important for tumor growth and ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:
更新日期:2009-02-18 00:00:00
abstract::Tamoxifen was the first targeted anticancer agent for breast cancer patients and its effects on reduction of breast cancer events and improvement in overall survival are undisputed. Hence, it has long been considered an essential part of patient care. Recent results of several large adjuvant hormonal trials evaluating...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S10155
更新日期:2010-12-17 00:00:00
abstract:BACKGROUND:Experimental disruption of the telomere overhang induces a potent DNA damage response and is the target of newly emerging cancer therapeutics. Introduction of T-oligo, an eleven-base oligonucleotide homologous to the 3'-telomeric overhang, mimics telomere disruption and induces DNA damage responses through a...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S54575
更新日期:2013-12-18 00:00:00
abstract:OBJECTIVE:To investigate the impact of surgical resection extent on the postoperative complications and the prognosis in patients with stage II endometrial cancer. METHODS:A total of 54 patients were retrospectively reviewed, 35 patients underwent subradical hysterectomy and 19 patients received radical hysterectomy, ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S95806
更新日期:2016-02-16 00:00:00
abstract:BACKGROUND:In patients with gastric cancer (GC), survival is poor, given the late diagnosis. Risk-stratifying these patients earlier could help improve care. We determined whether combining preoperative albumin concentration and the neutrophil lymphocyte ratio (COA-NLR) could predict overall survival (OS) better than o...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S108631
更新日期:2016-07-27 00:00:00
abstract::MicroRNAs (miRs) have been demonstrated to play key roles in the development and progression of hepatocellular carcinoma (HCC). However, the regulatory mechanism of miR-503 in HCC has not been fully uncovered. In this study, we found that miR-503 was significantly downregulated in HCC tissues compared to nontumorous l...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S106351
更新日期:2016-06-15 00:00:00
abstract::[This corrects the article DOI: 10.2147/OTT.S262089.]. ...
journal_title:OncoTargets and therapy
pub_type: 已发布勘误
doi:10.2147/OTT.S292577
更新日期:2020-11-27 00:00:00
abstract:PURPOSE:Although numerous studies have demonstrated associations between the preoperative neutrophil-to-lymphocyte ratio (NLR) and long-term outcomes in patients with non-small cell lung cancer (NSCLC), the prognostic significance of postoperative NLR and change in NLR (ΔNLR) is unknown for patients who underwent compl...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S117290
更新日期:2016-10-21 00:00:00
abstract:BACKGROUND:Due to the hypervascular nature of glioblastoma (GBM), antiangiogenic treatments, such as vatalanib, have been added as an adjuvant to control angiogenesis and tumor growth. However, evidence of progressive tumor growth and resistance to antiangiogenic treatment has been observed. To counter the unwanted eff...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S93790
更新日期:2016-03-09 00:00:00
abstract::Immune cells are essential for defending the body's balance and have increasingly been implicated in controlling tumor growth. In cervical cancer (CC), the immune landscape is extensively connected with human papillomavirus (HPV) status. Recent insights from studies have revealed that as a result of infection with HPV...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S264312
更新日期:2020-08-12 00:00:00
abstract:Introduction:Disulfiram (DSF), a drug used in the treatment of alcoholism since 1948, has been shown to have antitumor properties in various tumor types possibly due to the induction of a type cell death, ferroptosis, and the sensitization of cells to chemo- and radiotherapy. In this study, we explored the antitumor pr...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S272312
更新日期:2020-10-20 00:00:00
abstract:AIM:This article discusses the current use of volumetric modulated arc therapy (VMAT) techniques in clinical practice and reviews the available data from clinical outcome studies in different clinical settings. An overview of available literature about clinical outcomes with VMAT stereotactic/radiosurgical treatment is...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S113119
更新日期:2017-07-26 00:00:00
abstract::Primary urethral non-Hodgkin's lymphoma (NHL) is uncommon. This case study reports the case of a 52-year-old woman with a light red mass at the urethral orifice. Her clinical manifestations included frequent urination, urgency, dysuria, and occasionally blood in urine. The tumor was surgically removed, and the patholo...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S283704
更新日期:2020-12-21 00:00:00
abstract:Purpose:The aim was to investigate resveratrol effects on A549 cells proliferation. Methods:A total of 104 lung adenocarcinoma tissues and nontumor tissues were collected. BEAS-2B cells were cultured in RPMI 1640 medium (group A). A549 cells were treated with RPMI 1640 medium containing different resveratrol concentra...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S157613
更新日期:2018-05-22 00:00:00
abstract::Over the last decades, a better understanding of breast cancer heterogeneity provided tools for a biologically based personalization of anticancer treatments. In particular, the overexpression of the human epidermal growth factor receptor 2 (HER2) by tumor cells provided a specific target in these HER2-positive tumors...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S122397
更新日期:2017-07-10 00:00:00
abstract:OBJECTIVES:This study aimed to analyze incidence and mortality trends in breast cancer (BC), corpus uteri cancer (CUC), and ovarian cancer (OC) in Poland in the context of sociodemographic changes. MATERIALS AND METHODS:Incidence and mortality data (1980-2013) were retrieved from the Polish National Cancer Registry, w...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S112187
更新日期:2016-09-07 00:00:00
abstract:PURPOSE:Randomized trials showing a clear survival benefit of intensity-modulated radiotherapy (IMRT) over 3-dimensional conformal radiotherapy (3D-CRT) in the treatment of lung cancer are lacking. This study compared the survival rates of patients with stage III non-small cell lung cancer who were treated with either ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S124311
更新日期:2016-11-24 00:00:00