Abstract:
Background:It was previously reported that downregulation of miR-218 promoted thyroid cancer cell invasion, migration, and proliferation. However, the biological functions of miR-218 and its possible regulatory mechanisms in papillary thyroid cancer (PTC) cells are still elusive. Materials and methods:The expression levels of miR-218 and Runx2 in PTC tissues and cells were determined by quantitative real-time PCR (qRT-PCR) and Western blot. The effects of miR-218 overexpression on cell viability, invasion, apoptosis, and PTEN/PI3K/AKT pathway in PTC cells were evaluated by cell counting kit-8 assay, Transwell invasion assay, flow cytometry assay, and Western blot, respectively. Luciferase reporter assay and qRT-PCR were performed to identify the target of miR-218. Xenograft tumor experiment was performed to confirm the biological roles of miR-218 and its potential mechanisms in vivo. Results:miR-218 expression was downregulated and Runx2 expression was upregulated in PTC tissues and cells. Overexpression of miR-218 suppressed viability and invasion, and induced apoptosis of PTC cells in vitro, while Runx2 overexpression greatly abolished these effects. miR-218 overexpression inactivated the PTEN/PI3K/AKT pathway, which was abated by Runx2 upregulation. Additionally, Runx2 was validated to be a direct target of miR-218. Moreover, enforced expression of miR-218 inhibited tumor growth and Runx2 expression, and blocked PTEN/PI3K/AKT pathway in vivo. Conclusion:miR-218 overexpression suppresses the tumorigenesis of PTC via downregulating PTEN/PI3K/AKT pathway by targeting Runx2, which indicates that miR-218 may be a potential therapeutic target for human PTC.
journal_name
Onco Targets Therjournal_title
OncoTargets and therapyauthors
Han M,Chen L,Wang Ydoi
10.2147/OTT.S172152subject
Has Abstractpub_date
2018-09-28 00:00:00pages
6305-6316issn
1178-6930pii
ott-11-6305journal_volume
11pub_type
杂志文章abstract::Several members of the sirtuin (SIRT) family, a highly conserved family of NAD+-dependent enzymes, have been shown to play a critical role in both promoting and/or suppressing tumorigenesis. In this study, recent progress in the field concerning SIRT4 and cancer was reviewed, and the relationship between SIRT4 and tum...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S157724
更新日期:2018-06-11 00:00:00
abstract:Purpose:Stomach cancer is one of the highest incidence and mortality malignancies worldwide. Our study aimed to illustrate the somatic mutation landscape and identify molecular markers of stomach cancer. Materials and Methods:By integrated analysis of sequencing data and clinical data of stomach adenocarcinoma (STAD) ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S263733
更新日期:2020-08-04 00:00:00
abstract::Orbital metastasis as the initial presentation of lung adenocarcinoma is very rare, and so the lack of knowledge about this phenomenon can easily result in misdiagnosis, either as a orbital primary tumor or benign lesion. Here, we report a rare case in which the orbital symptom appeared first without any pulmonary man...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S99583
更新日期:2016-05-09 00:00:00
abstract:Purpose:Circular RNAs (circRNAs) have been found to regulate several human tumors. The present study was to explore the mechanism of hsa_circ_0087862 in regulating non-small cell lung cancer (NSCLC). Methods:Totally 102 NSCLC cases were enrolled. NCI-H1359 and A549 cells were transfected. Cells viability, apoptosis, m...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S243533
更新日期:2020-04-03 00:00:00
abstract:BACKGROUND:Leucine-rich glioma inactivated 3 (LGI3) is a secreted protein member of LGI family. We previously reported that LGI3 was expressed in brain, adipose tissues and skin, where it played roles as a multifunctional cytokine. We postulated that LGI3 may be involved in cytokine network in cancers. AIM:This study ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S138912
更新日期:2017-05-24 00:00:00
abstract::Tbx2 is a cancer-related protein that was found to be overexpressed in several human malignancies. The present study aims to investigate the clinical significance and biological role of Tbx2 in human astrocytoma. We examined its protein expression in 102 cases of astrocytoma tissues using immunohistochemical staining....
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S124012
更新日期:2017-02-20 00:00:00
abstract::Neuroendocrine tumors are a rare and heterogeneous group of tumors with a variety of primary origins and variable aggressiveness. Platinum-based chemotherapy has been the cornerstone of treatment for the poorly differentiated tumors. However, well-differentiated neuroendocrine tumors are quite chemoresistant and thera...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S72155
更新日期:2016-02-09 00:00:00
abstract::Erastin was initially discovered as a small molecule compound that selectively kills tumor cells expressing ST and RASV12 and was later widely investigated as an inducer of ferroptosis. Ferroptosis is a recently discovered form of cell death caused by peroxidation induced by the accumulation of intracellular lipid rea...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S254995
更新日期:2020-06-11 00:00:00
abstract:Introduction:Because only a small portion of NSCLC (non-small-cell lung cancer) patients benefit from molecular targeted therapy or immunotherapy and do not develop therapeutic resistance, continued research on new targets is warranted. Serotonin has recently emerged as a growth factor for tumor cells, and its receptor...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S244339
更新日期:2020-03-09 00:00:00
abstract::[This corrects the article DOI: 10.2147/OTT.S196910.]. ...
journal_title:OncoTargets and therapy
pub_type: 已发布勘误
doi:10.2147/OTT.S229714
更新日期:2019-09-19 00:00:00
abstract::Androgen deprivation therapy (ADT) is well established as a backbone therapy for metastatic prostate cancer (mPCa), and both European and American guidelines emphasize the importance of maintaining ADT after progression to metastatic castration-resistant prostate cancer (CRPC). However, the use of ADT varies widely in...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S117176
更新日期:2016-11-29 00:00:00
abstract:BACKGROUND:Two types of epidermal growth factor receptor (EGFR) mutations in exon 19 and exon 21 (ex19del and L858R) are prevalent in lung cancer patients and sensitive to targeted EGFR inhibition. A resistance mutation in exon 20 (T790M) has been found to accompany drug treatment when patients relapse. These three mut...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S84938
更新日期:2015-06-22 00:00:00
abstract::The association between polymorphic CAG repeats in the androgen receptor gene in women and breast cancer susceptibility has been studied extensively. However, the conclusions regarding this relationship remain conflicting. The purpose of this meta-analysis was to identify whether androgen receptor CAG repeat lengths w...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S85130
更新日期:2015-08-13 00:00:00
abstract:Background:The diagnosis rate of early stage esophageal squamous cell carcinoma (ESCC) is low due to the lack of specific tumor markers. Seeking for these markers is beneficial to improve the early diagnosis rate and the prognosis of patients. This study profiles the differentially expressed proteins of early stage ESC...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S171242
更新日期:2018-09-12 00:00:00
abstract::The epidermal growth-factor receptor (EGFR) is overexpressed in the majority of epithelial ovarian cancers and promotes cell proliferation, migration and invasion, and angiogenesis, as well as resistance to apoptosis. This makes EGFR an attractive therapeutic target in this disease. A number of strategies to block EGF...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S30373
更新日期:2013-04-18 00:00:00
abstract:BACKGROUND:MDS1 and EVI1 complex locus protein EVI1 (MECOM) is an oncogenic transcription factor in several kinds of cancers. However, the clinical significance of MECOM in glioblastoma multiforme (GBM) has not been well elucidated. PATIENTS AND METHODS:Our study enrolled 86 resected samples of GBM in three medical ce...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S95831
更新日期:2016-01-13 00:00:00
abstract:Introduction:A disintegrin and metallopeptidase with thrombospondin motifs (ADAMTSs), whose expression is dysregulated in various cancers, is implicated in cancer development. Herein, we aimed to investigate the functional role of ADAMTS8 in breast cancer (BC) and explore the underlying mechanisms. Methods:The protein...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S248085
更新日期:2020-08-21 00:00:00
abstract:Background:Insulin-like growth factor binding protein-2 (IGFBP2) levels are significantly increased in the plasma of hepatocellular carcinoma (HCC) patients. However, the correlation between IGFBP2 levels and clinical parameters and the exact role of IGFBP2 in HCC are unclear. In this study, we identified the role and ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S249527
更新日期:2020-06-08 00:00:00
abstract:AIM:To investigate the significance of epidermal growth factor receptor (EGFR), human epidermal growth factor receptor (HER)2, and HER3 expression on survival outcomes in Chinese gastric cancer patients. MATERIALS AND METHODS:Formalin-fixed, paraffin-embedded specimens from 121 patients who underwent gastrectomy at Sh...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S70922
更新日期:2014-12-16 00:00:00
abstract::Trastuzumab is a monoclonal antibody directed against the human epidermal growth factor receptor 2 (HER2). HER2 is amplified or overexpressed in about 15% of breast cancers and is associated with aggressive disease. Clinical benefits of trastuzumab have been established in the treatment of both early and metastatic HE...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S27733
更新日期:2013-01-01 00:00:00
abstract:Purpose:Breast cancer (BC) is the most common cancer in women. Emerging evidence has demonstrated that lncRNAs play an important role in BC. The objective of this study was to investigate the impact of the long non-coding RNA (lncRNA), H19/miRNA-130a-3P/special AT-rich sequence-binding protein-1 (SATB1) axis on BC prog...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S280142
更新日期:2020-12-07 00:00:00
abstract:Purpose:This study is aimed to investigate the combined treating efficacy of sodium butyrate and docetaxel on proliferation and apoptosis of the lung adenocarcinoma A549 cell line based on Gli1 regulation in vitro and in vivo. Materials and Methods:RNA interference method was used to overexpress Gli1 in A549 cells. Ce...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S252323
更新日期:2020-09-04 00:00:00
abstract:Background and aim:Standard chemotherapy has limited clinical efficacy in patients with esophageal cancer and there is a significant and unmet clinical need for effective treatment options for these patients. The aim of this study was to compare the clinical efficacy of the novel, targeted drug apatinib combined with d...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S191736
更新日期:2019-04-08 00:00:00
abstract:Purpose:Hepatocellular carcinoma (HCC) has a high incidence in China and exploring effective ways for early diagnosis is an important method to improve the prognosis of patients with HCC. Additional studies reported that. Some kinds of microRNA (miRNA) in plasma will change accordingly during HCC progress, and this cha...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S232453
更新日期:2020-03-09 00:00:00
abstract:Purpose:Circular RNAs (circRNAs) are emerging as promising biomarkers for various human malignancies. However, the application of circRNAs as non-invasive biomarkers in high-grade serous ovarian cancer (SOC) remains to be elucidated. Here, we aim to investigate the feasibility of using serum circSETDB1, a tumor-promoti...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S220700
更新日期:2019-09-11 00:00:00
abstract:BACKGROUND:The PI3K family participates in multiple signaling pathways to regulate cellular functions. PI3K/Akt signaling pathway plays an important role in tumorigenesis and development. O-GlcNAcylation, a posttranslational modification, is thought to modulate a wide range of biological processes, such as transcriptio...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S82845
更新日期:2015-11-09 00:00:00
abstract::This retrospective study was conducted to evaluate the prognostic significance of the preoperative plasma D-dimer levels and platelet counts in patients with pancreatic adenocarcinoma. A total of 168 consecutive locally advanced pancreatic adenocarcinoma patients who underwent intensity modulated radiation therapy wit...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S82329
更新日期:2015-06-04 00:00:00
abstract::Malignant pericardial effusion (MPCE) is a common complication of advanced malignant tumors, and interferes severely with patient prognosis and quality of life. The standard treatment for this complication is intracavitary perfusion of chemotherapeutic drugs, which is limited by unsatisfactory therapeutic effects and ...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S90145
更新日期:2015-10-01 00:00:00
abstract:Objective:Growing epidemiological evidence supports that coagulation cascades and cancer-associated inflammation are associated with recurrence and survival of epithelial ovarian cancer (EOC). This study aimed to assess the clinical significance of the combination of plasm fibrinogen and neutrophil lymphocyte ratio (F-...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S264118
更新日期:2020-07-27 00:00:00
abstract:Background:The aberrant expression of circular RNAs (circRNAs) has been identified as a novel trait of cancers. However, the role of circRNAs in colorectal cancer (CRC) remains to be elucidated. Methods:Informatic analysis was performed to identify circRNAs in CRC tissues and adjacent tissues. Gain- and loss-of-functi...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S255485
更新日期:2020-06-30 00:00:00