Abstract:
Background:Nicotinamide phosphoribosyltransferase (NAMPT) and the transforming growth factor-β (TGF-β) signaling pathway play important roles in colorectal tumorigenesis and progress. However, the underlying regulatory mechanisms between NAMPT and TGF-β signaling in colorectal cancer (CRC) remain poorly understood. Methods:Public data were extracted from the Oncomine database and the PrognoScan database to investigate the mRNA expression and the prognostic value of NAMPT, respectively, in CRC. Western blot tests were performed to detect Smad2, Smad3, p-Smad2, p-Smad3, Smad4 expression in CRC cells transfected with human NAMPT-siRNA or NAMPT-overexpressing plasmid. TGF-β1 concentrations in culture supernatants were assayed using ELISA kits. The effect of TGF-β1 on NAMPT expression was evaluated by quantitative real-time PCR and Western blot. The dual-luciferase reporter assay was employed to confirm the binding of miR-1-3p to NAMPT 3'-UTR. Subsequently, NAMPT levels in HCT116 cells transfected with the mimics and inhibitors of miR-1-3p were detected by quantitative real-time PCR and Western blot. Results:NAMPT was overexpressed in human CRC and was correlated with short overall survival. NAMPT increased the protein expression levels of components in the TGF-β signaling pathway including Smad2, Smad3, and Smad4. Moreover, NAMPT promoted TGF-β1 secretion. Intriguingly, the TGF-β1 treatment down-regulated NAMPT expression at mRNA and protein levels in CRC cells which were partly through the up-regulation of miR-1-3p that directly bound to the NAMPT 3'-UTR. These outcomes demonstrated that NAMPT was a downstream target of miR-1-3p and there was a negative association between NAMPT and miR-1-3p in CRC. Conclusion:There is a negative feedback loop between NAMPT and the TGF-β signaling pathway in CRC cells, providing new insight into the mechanism underlying the regulatory pathways in CRC.
journal_name
Onco Targets Therjournal_title
OncoTargets and therapyauthors
Lv X,Zhang J,Zhang J,Guan W,Ren W,Liu Y,Xu Gdoi
10.2147/OTT.S282367subject
Has Abstractpub_date
2021-01-08 00:00:00pages
187-198issn
1178-6930pii
282367journal_volume
14pub_type
杂志文章abstract:Background:The aberrant expression of circular RNAs (circRNAs) has been identified as a novel trait of cancers. However, the role of circRNAs in colorectal cancer (CRC) remains to be elucidated. Methods:Informatic analysis was performed to identify circRNAs in CRC tissues and adjacent tissues. Gain- and loss-of-functi...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S255485
更新日期:2020-06-30 00:00:00
abstract::During the past decade, immunotherapy targeting immune checkpoints has become an important component of the treatment paradigm for numerous malignancies, especially PD-1/PD-L1 blockade which was demonstrated to rejuvenate disabled T cells in cancer patients to achieve long-term remissions. However, the clinical outcom...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S221340
更新日期:2019-10-15 00:00:00
abstract::Despite advances in the treatment of multiple myeloma, it remains an incurable disease, with relapses and resistances frequently observed. Recently, immunotherapies, in particular, monoclonal antibodies, have become important treatment options in anticancer therapies. Elotuzumab is a humanized monoclonal antibody to s...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S94531
更新日期:2016-10-05 00:00:00
abstract::Quercetin is proven to have anticancer effects for many cancers. However, the role of tumor suppressor p53 on quercetin's radiosensitization and regulation of endoplasmic reticulum (ER) stress response in this process remains obscure. Here, quercetin exposure resulted in ER stress, prolonged DNA repair, and the expres...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S147316
更新日期:2017-12-21 00:00:00
abstract:Background:PD-1 checkpoint inhibitors have shown a robust tumor response in the treatment of various cancers. Pembrolizumab is an anti-PD-1 checkpoint antibody approved for the treatment of unresectable or metastatic melanoma in more than 40 countries. Although autoimmune pneumonitis is considered a common immune-relat...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S178246
更新日期:2018-10-24 00:00:00
abstract:Background:Gastric tumor (GT) is associated with high morbidity and mortality, with surgery among the most effective treatment methods. Accurate interoperative determination of the tumor margin is crucial. Methods:In this study, using internal extractive electrospray ionization-MS, mass spectral data of GT and gastric...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S221589
更新日期:2019-10-17 00:00:00
abstract::This study tested the potential of circulating RNA-based signals as predictive biomarkers for docetaxel response in patients with metastatic castration-resistant prostate cancer (CRPC). RNA was analyzed in blood from six CRPC patients by whole-transcriptome sequencing (total RNA-sequencing) before and after docetaxel ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S86637
更新日期:2015-07-30 00:00:00
abstract:Introduction:Multiple myeloma (MM) remains an incurable disease, and patient survival requires a better understanding of this malignancy's molecular aspects. Heparanase (HPSE) is highly expressed in aggressive MM cells and related to tumor growth, metastasis, and bortezomib (BTZ) resistance. Thus, targeting HPSE seems ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S286751
更新日期:2021-01-15 00:00:00
abstract::Targeted radioimmunotherapy in non-Hodgkin's B-cell lymphoma (NHL) offers an efficacious therapy and minimal toxicity compared to conventional chemotherapy. Iodine 131 tositumomab ((131)I-TST) is a murine monoclonal antibody against the CD20 cell surface protein and is directly covalently conjugated to (131)I, a radio...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/ott.s4456
更新日期:2009-02-18 00:00:00
abstract:BACKGROUND:The purpose of this study was to determine the efficacy of sorafenib in preventing and treating tumor recurrence after liver transplantation in patients with primary hepatic carcinoma exceeding the Milan criteria. METHODS:Thirty patients with primary hepatic carcinoma exceeding the Milan criteria underwent ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S31387
更新日期:2012-01-01 00:00:00
abstract::Normal astrocytes are more resistant to radiation than glioma cells. Radiation-resistant glioma cells and normal astrocytes usuallly share similar mechanisms of radioresistance. Investigation of the underlying mechanisms of differential radiation response between normal astrocytes and glioma cells is thus significant ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S144002
更新日期:2017-12-04 00:00:00
abstract::Breast cancer is one of the most common malignant cancers among women and a major clinical obstacle. Although studies have reported the abnormal expression of SIRT7 in breast cancer, whether the function of SIRT7 regulates the expression of long noncoding RNAs (lncRNAs) in breast cancer remains unknown. We aimed to de...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S149048
更新日期:2017-10-24 00:00:00
abstract:Objective:The biological functions and molecular mechanisms of miR-935 have been widely investigated in various types of cancer. The aim of the present study was to explore the function of miR-935 in glioma. Methods:Bioinformatic analysis and quantitative real-time fluorescent PCR (qRT-PCR) were used to determine the ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S244409
更新日期:2020-10-23 00:00:00
abstract:Background:Bone marrow stromal antigen 2 (BST2) is considered as a transmembrane glycoprotein and plays essential roles in innate immunity. It has been recently reported that up-regulation of BST2 was associated with the development of breast carcinoma. However, the clinical significance of BST2 in glioma has not been ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S258631
更新日期:2020-08-26 00:00:00
abstract:OBJECTIVE:Even though the primary mediastinal extragonadal germ cell tumors (EGCTs) are rare, they are noteworthy in the differential diagnosis of mediastinal masses. In this study, we aimed to identify the clinical features of mediastinal malignant GCTs and compare the results of hematopoietic stem cell transplantatio...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S107899
更新日期:2016-12-09 00:00:00
abstract::Ovarian cancer (OC) is the most lethal cancer of all gynecological malignancies, while endometrial cancer (EC) is the most common one. Current strategies for OC/EC diagnosis consist of the extraction of a solid tissue from the affected area. This sample enables the study of specific biomarkers and the genetic nature o...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S227156
更新日期:2019-12-27 00:00:00
abstract:Purpose:LINC00680 was reported to be involved in various cancers through multiple mechanisms. Therefore, we intended to investigate its role in the progression of non-small cell lung cancer (NSCLC). Materials and Methods:Firstly, quantitative real-time polymerase chain reaction (qRT-PCR) was used to test LINC00680 in ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S259232
更新日期:2020-08-18 00:00:00
abstract::Dichloroacetate (DCA), a small molecule mitochondria-targeting agent, can penetrate the blood-brain barrier, showing potential therapeutic effects on brain tumors. Considering the effects of DCA on tumor cellular metabolism, penetrating across the blood-brain barrier, as well as having potential antitumor activity on ...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S40992
更新日期:2013-01-01 00:00:00
abstract:Background:The non-receptor tyrosine kinase Fyn-related kinase (FRK) has been reported to affect cell proliferation in several cancer types. However, its effect on the proliferation of clear cell renal cell carcinoma (ccRCC) remains largely unknown. Purpose:The objective of this study was to investigate the expression...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S199238
更新日期:2019-04-10 00:00:00
abstract::Increasing evidence has shown that aldehyde dehydrogenase 1A1 (ALDH1A1), a detoxifying enzyme, is responsible for chemoresistance in a variety of tumors. Although the majority of patients with diffuse large B-cell lymphoma (DLBCL) can be cured with cyclophosphamide, doxorubicin, vincristine, and prednisone (CHOP), che...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S107957
更新日期:2016-08-29 00:00:00
abstract::Giant-cell carcinoma of the lung (GCCL) is a rare histological form of poorly differentiated non-small-cell lung cancer, which is classified as a subtype of pulmonary sarcomatoid carcinomas. In this case report, we describe the case of a 50-year-old Chinese male who presented with a pulmonary nodule in the right upper...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S150124
更新日期:2018-02-09 00:00:00
abstract:Introduction:Esophageal squamous cell carcinoma (ESCC) is a malignant tumor disease with high mortality and morbidity rates, especially for a terminal cancer. At present, the prognosis and treatment of ESCC cannot effectively control or inhibit the spread and proliferation of tumor cells. microRNAs, a class of small sp...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S248925
更新日期:2020-08-13 00:00:00
abstract::Background: Carcinoma ex pleomorphic adenoma (CXPA), a very rare malignancy found mostly in the major salivary glands, has no established standardized treatment. Case presentation: This report describes a 67-year-old male with advanced CXPA who was effectively treated by anlotinib. Pleomorphic adenoma of the submandib...
journal_title:OncoTargets and therapy
pub_type:
doi:10.2147/OTT.S200324
更新日期:2019-05-24 00:00:00
abstract:Purpose:The TRMT12 is a novel oncogene involved in breast cancer. However, the association between TRMT12 and head and neck squamous cell carcinoma (HNSCC) remains unclear. Materials and methods:The levels of TRMT12 mRNA in HNSCC and normal tissues were analyzed using data from the Cancer Genome Atlas-HNSC. The expres...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S212200
更新日期:2019-09-05 00:00:00
abstract::The study is designed to explore the correlations of microvascular blood flow of contrast-enhanced ultrasound (CEUS) and hepatocyte growth factor (HGF)/c-Met signaling pathway with clinicopathological features and prognosis of patients with hepatocellular carcinoma (HCC). One hundred and eighteen patients pathological...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S113353
更新日期:2017-02-14 00:00:00
abstract:BACKGROUND:Associations between Arg399Gln single-nucleotide polymorphism (SNP) in the XRCC1 gene and leukemia susceptibility have been studied extensively, however, the results are inconsistent. The aim of this study was to determine these associations using meta-analytical methods. METHODS:A meta-analysis was perform...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S92752
更新日期:2015-11-06 00:00:00
abstract:Purpose:Aberrant activation of the Janus-associated kinase (JAK)/signal transducer and activator of transcription (STAT) pathway is associated with increased malignant cell proliferation and survival. This Phase Ib study evaluated ruxolitinib, a potent JAK1/2 inhibitor, in combination with gemcitabine with or without n...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S157331
更新日期:2018-04-30 00:00:00
abstract:Objective:Osteoclastogenesis is a key process in osteolytic bone metastasis (BM). Previous studies indicated that some miRNAs could regulate cancers progression and osteoclastogenesis. Our purpose was to investigate the roles of lung cancer cells-derived circulating miR-21 on osteoclastogenesis and its clinical signifi...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S232876
更新日期:2020-03-31 00:00:00
abstract:Introduction:Forkhead box (FOX) superfamily members were recently shown to play important roles in tumor development and progression. Forkhead box S1 (FOXS1), a member of the FOX family, has been reported to be closely associated with malignant neoplasms. However, its expression and effect on hepatocellular carcinoma r...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S272596
更新日期:2020-11-17 00:00:00
abstract:Background:Non-small-cell lung cancer (NSCLC) is the major leading cause of cancer-related death around the world. The resistance to chemotherapy limits the effects of clinical treatment. The aim of this study was to identify novel mechanisms involved in NSCLC chemoresistance. Materials and methods:We explored the pub...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S175606
更新日期:2018-09-26 00:00:00