Abstract:
Introduction:Esophageal squamous cell carcinoma (ESCC) is a malignant tumor disease with high mortality and morbidity rates, especially for a terminal cancer. At present, the prognosis and treatment of ESCC cannot effectively control or inhibit the spread and proliferation of tumor cells. microRNAs, a class of small spliced RNAs, are essential in the regulation of tumorigenesis and tumor cell migration and proliferation. microRNAs interact with target mRNA to silence gene expression and degrade mRNA, thereby inhibiting the expression of tumor genes or impairing the expression of tumor suppressor genes. Methods:A total of 20 human ESCC samples were collected from the Affiliated Tumor Hospital of Xinjiang Medical University. Eca109 and Kyse510 cells, which are ESCC cell lines, were subjected to FACS analysis to get side population (SP) cells and non-SP cells. Cell cycle and cell proliferation were analyzed by flow cytometry. Cell migration and invasion were detected using a transwell assay. Quantitative PCR and Western blot were performed to analyze the expression levels of ABCG2, KLF4, OCT4, and ACVR1, which are related to the stemness of stem cells. The target genes of hsa-miR-148a were predicted using TargetScan (version 7.2) and verified by a dual luciferase reporter assay. A chromatin immunoprecipitation (ChIP) assay was carried out to demonstrate direct interaction between miR-148a and ACVR1. Results:The expression of miR-148a was significantly down-regulated in ESCC cells and significantly decreased in SP esophageal squamous cells when compared to the tumor cells. By analyzing the stem cell stemness of ESCC, overexpression of miR-148a decreased the expression of ABCG2, KLF4, SOX2, OCT4, and Nanog, indicating that miR-148a may regulate stem cell function. Target gene prediction and functional annotation of miR-148a suggested that miR-148a is involved in stem cell stemness of ESCC via ACVR1. Expression of the dual luciferase-labeled gene indicates that overexpression of miR-148a inhibits the expression of ACVR1, thereby affecting stem cell stemness. Conclusion:miR-148a regulates the stem cell-like side populations distribution by inhibiting the expression of ACVR1 in ESCC. miR-148a may be a promising targeted therapy for ESCC.
journal_name
Onco Targets Therjournal_title
OncoTargets and therapyauthors
Tan Y,Lu X,Cheng Z,Pan G,Liu S,Apiziaji P,Wang H,Zhang J,Abulimiti Ydoi
10.2147/OTT.S248925subject
Has Abstractpub_date
2020-08-13 00:00:00pages
8079-8094issn
1178-6930pii
248925journal_volume
13pub_type
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journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
doi:10.2147/OTT.S141053
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journal_title:OncoTargets and therapy
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journal_title:OncoTargets and therapy
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journal_title:OncoTargets and therapy
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journal_title:OncoTargets and therapy
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journal_title:OncoTargets and therapy
pub_type: 杂志文章
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abstract:BACKGROUND:"Gefitinib" is a first-generation epidermal growth factor receptor tyrosine-kinase inhibitor. More than half of patients receiving gefitinib develop acne-like eruption. Evozac(®) Calming Skin Spray (Evaux Laboratoires, Évaux-les-Bains, France) is made of Évaux thermal spring water and commonly used for the t...
journal_title:OncoTargets and therapy
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journal_title:OncoTargets and therapy
pub_type: 杂志文章
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journal_title:OncoTargets and therapy
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journal_title:OncoTargets and therapy
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journal_title:OncoTargets and therapy
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journal_title:OncoTargets and therapy
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journal_title:OncoTargets and therapy
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journal_title:OncoTargets and therapy
pub_type: 杂志文章
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journal_title:OncoTargets and therapy
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doi:10.2147/OTT.S246841
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journal_title:OncoTargets and therapy
pub_type: 杂志文章
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更新日期:2017-02-20 00:00:00
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journal_title:OncoTargets and therapy
pub_type: 杂志文章
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journal_title:OncoTargets and therapy
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journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S84888
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journal_title:OncoTargets and therapy
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journal_title:OncoTargets and therapy
pub_type: 撤回出版物
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journal_title:OncoTargets and therapy
pub_type: 杂志文章
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journal_title:OncoTargets and therapy
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更新日期:2016-12-19 00:00:00
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journal_title:OncoTargets and therapy
pub_type: 杂志文章
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journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S130905
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abstract::Gastric cancer (GC) is one of the leading causes of tumor-related mortality. In addition to surgery and endoscopic resection, systemic therapy remains the main treatment option for GC, especially for advanced-stage disease and for cases not suitable for surgical therapy. Hence, improving the efficacy of systemic thera...
journal_title:OncoTargets and therapy
pub_type: 杂志文章,评审
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abstract:Background:Circular RNAs (circRNAs) are a special class of noncoding RNAs that are involved in gene regulation and compete with mRNA for miRNA binding sites. The roles of circRNAs in cancer, especially breast cancer (BC), are poorly understood. Materials and Methods:The expression levels of circRNA 0001073 (circ-1073)...
journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S248822
更新日期:2020-06-29 00:00:00
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journal_title:OncoTargets and therapy
pub_type:
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更新日期:2018-10-23 00:00:00
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journal_title:OncoTargets and therapy
pub_type: 杂志文章
doi:10.2147/OTT.S230678
更新日期:2020-02-12 00:00:00
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journal_title:OncoTargets and therapy
pub_type: 杂志文章
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