Abstract:
Purpose:To investigate the occurrence and genetic characteristics of the blaIMP-26-positive plasmid from a multidrug-resistant clinical isolate, Enterobacter hormaechei L51. Methods:Species identification was determined by MALDI-TOF MS and Sanger sequencing. Antimicrobial susceptibility testing was performed by the agar dilution and broth microdilution. Whole-genome sequencing was conducted using Illumina HiSeq 4000-PE150 and PacBio Sequel platforms, and the genome was annotated by the RAST annotation server. The ANI analysis of genomes was performed using OAT. Phylogenetic reconstruction and analyses were performed using the Harvest suite based on the core-genome SNPs of 61 publicly available E. hormaechei genomes. Results:The E. hormaechei L51 genome consists of a 5,018,729 bp circular chromosome and a 343,918 bp conjugative IncHI2/2A plasmid pEHZJ1 encoding blaIMP-26 which surrounding genetic context was intI1-blaIMP-26-ltrA-qacE∆1-sul1. A new sequence type (ST1103) was assigned for the isolate L51 which was resistant to cephalosporins, carbapenems, but sensitive to piperacillin-tazobactam, amikacin, tigecycline, trimethoprim-sulfamethoxazole and colistin. Phylogenetic analysis demonstrated that E. hormaechei L51 belonged to the same subspecies as the reference strain E. hormaechei SCEH020042, however 18,248 divergent SNP were identified. Resistance genes in pEHZJ1 including aac(3)-IIc, aac(6')-IIc, blaSHV-178, blaDHA-1, blaTEM-1, blaIMP-26, ereA2, catII, fosA5, qnrB4, tet(D), sul1 and dfrA19. Conclusion:In our study, we identified a conjugative IncHI2/2A plasmid carrying blaIMP-26 and blaSHV-178 in E. hormaechei ST1103, a novel multidrug-resistant strain isolated from China, and describe the underlying resistance mechanisms of the strain and detailed genetic context of mega plasmid pEHZJ1.
journal_name
Infect Drug Resistjournal_title
Infection and drug resistanceauthors
Gou JJ,Liu N,Guo LH,Xu H,Lv T,Yu X,Chen YB,Guo XB,Rao YT,Zheng BWdoi
10.2147/IDR.S232514subject
Has Abstractpub_date
2020-02-20 00:00:00pages
597-605issn
1178-6973pii
232514journal_volume
13pub_type
杂志文章abstract:Background:Neonatal sepsis (NS) is one of the leading causes of infant morbidity and mortality, but little is known about pathogen incidence and distribution in China. Methods:In this retrospective study (January 2012 to December 2016), culture-proven cases aged less than 28 days with diagnosed NS in the Guangzhou Wom...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S223597
更新日期:2019-12-03 00:00:00
abstract:Background:The timely and accurate diagnosis of infected nonunion is challenging, and there is a need for more efficient biomarkers. Previous studies have shown that fibrinogen plays an important role in mediating inflammation in bacterial infections and, therefore, could be a valuable biomarker for infected nonunion. ...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S269719
更新日期:2020-11-04 00:00:00
abstract::Vancomycin has usually been associated with nephrotoxicity. Generally, this toxicity is presented as proximal tubular cells injury with or without necrosis and as acute interstitial nephritis. However, development of both lesions is uncommonly described in literature. We present a case of vancomycin-induced nephrotoxi...
journal_title:Infection and drug resistance
pub_type:
doi:10.2147/IDR.S171669
更新日期:2018-07-30 00:00:00
abstract::Mycoplasma genitalium is one of the important causes of non-gonococcal urethritis. Rising incidence and emerging antimicrobial resistance are a major concern these days. The poor clinical outcomes with doxycycline therapy led to the use of azithromycin as the primary drug of choice. Single-dose azithromycin regimen ov...
journal_title:Infection and drug resistance
pub_type: 杂志文章,评审
doi:10.2147/IDR.S105469
更新日期:2017-09-01 00:00:00
abstract:Purpose:This study examined patient- and hospital-level predictor variables that contribute to worse clinical and economic outcomes in patients with carbapenem-nonsusceptible respiratory infections. Patients and Methods:Electronic data (January 2013 to September 2015) were from 78 US hospitals. Nonduplicate, gram-nega...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S236026
更新日期:2020-03-09 00:00:00
abstract:Background:Acinetobacter baumannii has emerged as an important pathogen associated with hospital- and community-acquired infections. Community-acquired A. baumannii pneumonia is characterized by a fulminant course and high mortality rates. In this study, we report the identification of a community-acquired carbapenem-r...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S202803
更新日期:2019-06-06 00:00:00
abstract:Aim:Plasmodium falciparum malaria predominantly affects children residing in endemic areas. However, recently a significant number of Malawian adults, who otherwise should have attained some malaria-specific immunity, have been observed to succumb to either uncomplicated malaria (UM) or severe malaria (SM). In addition...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S230112
更新日期:2020-01-07 00:00:00
abstract:Objective:To provide a comprehensive review of vancomycin dosing in patients with hematologic malignancies or neutropenia. Methods:PubMed, Embase and the Cochrane Library were searched through April 2, 2020. Original studies relevant to vancomycin dosing regimen in adults with hematologic malignancies or neutropenia w...
journal_title:Infection and drug resistance
pub_type: 杂志文章,评审
doi:10.2147/IDR.S239095
更新日期:2020-06-16 00:00:00
abstract:Background:China is one of the high-burden countries for multidrug-resistant tuberculosis (MDR-TB), and pyrazinamide is one of the anti-TB drugs used for the shorter MDR-TB treatment regimen. The aim of this study was to determine the correlation between pncA gene mutations and resistance to four first-line anti-TB dru...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S222943
更新日期:2019-09-30 00:00:00
abstract:Objective:Evaluate host and pathogen factors associated with mortality in those with hospital acquired infections (HAI) in a tertiary intensive care unit in Brazil. Methods:Observational and analytical cohort single center study in a general intensive care unit (ICU) in Northeastern Brazil between January 2016 and Aug...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S264276
更新日期:2020-08-12 00:00:00
abstract:Purpose:Infections, in particular with multidrug-resistant organisms, are a burden for inpatient and outpatient care and the whole community. The pathogens "roam" with patients and their relatives, forming an epidemiological bridge between different care facilities. Patients could play an important role in infection co...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S178922
更新日期:2018-12-04 00:00:00
abstract::Genetic and cellular studies have shown that the host's innate and adaptive immune responses are an important correlate of viral infection outcome. The features of the host's immune response (host resistance) reflect the coevolution between hosts and pathogens that has occurred over millennia, and that has also result...
journal_title:Infection and drug resistance
pub_type: 杂志文章,评审
doi:10.2147/IDR.S49891
更新日期:2015-04-07 00:00:00
abstract::The loss of polycationic antimicrobial peptides, polymyxins, due to adhesion to plastics is an important subject matter that influences in vitro susceptibility testing, including minimum inhibitory concentration (MIC) assays. This review reminds us that this issue can serve as a significant source of variation in the ...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S219130
更新日期:2019-08-27 00:00:00
abstract::A systematic review was conducted to determine the distribution and prevalence of antibiotic-resistant bacteria (ARB), antimicrobial-resistant genes (ARGs), and antimicrobial-resistant gene determinants (ARGDs) in clinical, environmental, and farm settings and to identify key knowledge gaps in a bid to contain their s...
journal_title:Infection and drug resistance
pub_type: 杂志文章,评审
doi:10.2147/IDR.S170715
更新日期:2018-10-17 00:00:00
abstract:Background:Ethiopia is one of the 22 high tuberculosis burden countries. In our country. there are limited published data to show the trend analysis of tuberculosis. Hence, we designed this trend analysis to fill the information gap in our study area. Institutional based retrospective cross-sectional study was employed...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S239717
更新日期:2020-02-24 00:00:00
abstract:Purpose:Complicated urinary tract infections (cUTIs) are among the most frequent health-care-associated infections. In patients with cUTI, Pseudomonas aeruginosa deserves special attention, since it can affect patients with serious underlying conditions. Our aim was to gain insight into the risk factors and prognosis o...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S185753
更新日期:2018-12-18 00:00:00
abstract::Lucio's phenomenon (LP) is a special reactional state associated with diffuse multibacillary leprosy; both exhibit a limitative global distribution mainly in Mexico and Central America. We report a case of a 28-year-old female leprosy patient in the People's Republic of China, together with LP and positive anticardiol...
journal_title:Infection and drug resistance
pub_type:
doi:10.2147/IDR.S202386
更新日期:2019-04-02 00:00:00
abstract:Background:The ability of biofilm formation is an effective way for Acinetobacter baumannii survival from stressed conditions. This present study was aimed to evaluate the association between biofilm formation, structure, the expression levels of genes related to biofilm formation and biofilm-specific resistance of A. ...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S228981
更新日期:2019-12-12 00:00:00
abstract:BACKGROUND:Biofilm formation inside inserted medical devices leads to their failure and acts as a source of refractory infections. The ultraviolet C (UVC) light is a potential therapy that can be used against the biofilm of bacterial pathogens. OBJECTIVE:We evaluated the efficacy of sublethal dose of UVC light with an...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S109343
更新日期:2016-08-19 00:00:00
abstract:Purpose:The activity of the cationic antimicrobial peptide WLBU2 was evaluated against planktonic cells and biofilms of multi-drug resistant (MDR) Acinetobacter baumannii and Klebsiella pneumoniae, alone and in combination with classical antimicrobial agents. Methods:Control American Type Culture Collection (ATCC) str...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S215084
更新日期:2019-07-09 00:00:00
abstract::Infections due to multidrug-resistant Gram-negative bacteria (MDR-GNB), especially when carbapenem resistant, have been very difficult to manage in the last fifteen years, owing to the paucity of dependable therapeutic options. Cefiderocol is a siderophore cephalosporin recently approved by the Food and Drug Administr...
journal_title:Infection and drug resistance
pub_type: 杂志文章,评审
doi:10.2147/IDR.S205309
更新日期:2020-12-29 00:00:00
abstract:Purpose:Antifungal resistance and virulence properties of Candida albicans (C. albicans) are growing health problems worldwide. The present study aims to investigate the effect of Zinc Oxide (ZnO) nanoparticles and Nystatin on SAP1-3 genes expression in C. albicans isolates of females with Vulvovaginal Candidiasis (VVC...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S226154
更新日期:2020-02-13 00:00:00
abstract::The New Delhi Metallo-β-lactamase (NDM) producing Enterobacteriaceae is spreading worldwide. Although the blaNDM gene has been identified in animal associated Enterobacteriaceae isolates in many countries, little is known about its occurrence in animal products in Pakistan. In this study, 13 Escherichia coli isolates ...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S236766
更新日期:2019-12-30 00:00:00
abstract:Introduction:Helicobacter pylori was discovered first in the stomachs of patients with gastritis and ulcers by Marshall and Warren in 1982. This discovery majorly affected many research areas of gastroenterology. Since then, the main aim has been to eradicate this microaerophilic bacterium from the stomachs of infected...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S223602
更新日期:2019-11-01 00:00:00
abstract:Purpose:The aim of this work was to identify a novel β-lactamase gene blaPAU-1 encoded on the plasmid of a clinical Pseudomonas aeruginosa isolate. Materials and methods:The clinical P. aeruginosa isolates were isolated from a hospital in southern China. Molecular cloning was performed to analyze the function of the r...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S225288
更新日期:2019-12-05 00:00:00
abstract::A lung abscess is an infectious pulmonary disease characterized by pus-filled cavity formation and often an air-fluid level. In this article, we described an indolent community-acquired lung abscess suspected as a tumor previously. A 56-year-old male presented with cough and expectoration for 2 months and hemoptysis f...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S218921
更新日期:2019-08-08 00:00:00
abstract:Background:Pseudomonas aeruginosa is an opportunistic pathogen that causes serious nosocomial infections, especially in immunodeficient patients and cystic fibrosis, cancer, and burned individuals. The biofilm that plays an important role in the virulence of P. aeruginosa is under the regulation of quorum sensing and t...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S263387
更新日期:2020-07-21 00:00:00
abstract:INTRODUCTION:In recent decades, antimicrobial resistance has become a public health problem, particularly in cases of healthcare-associated infections. Interaction between antibiotic consumption and resistance development is of particular interest regarding Gram-negative bacilli, whose growing resistance has represente...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S22491
更新日期:2011-01-01 00:00:00
abstract:Backgrounds:The aim of this study was to evaluate both phenotypic and genotypic determinants of mupirocin resistance among methicillin-resistant Staphylococcus aureus (MRSA) and methicillin susceptible S. aureus (MSSA) strains recovered from different clinical samples of children who were admitted to the Children's Med...
journal_title:Infection and drug resistance
pub_type: 杂志文章
doi:10.2147/IDR.S185610
更新日期:2019-01-03 00:00:00
abstract::While methicillin-resistant Staphylococcus aureus (MRSA) bacteremia has poor outcomes, we describe our experience with Ceftobiprole mainly as a combination therapy for the treatment of MRSA bacteremia. All the cases of MRSA bacteremia in our center at the King Abdulaziz Medical City (KAMC), Riyadh, that had undergone ...
journal_title:Infection and drug resistance
pub_type:
doi:10.2147/IDR.S254395
更新日期:2020-08-03 00:00:00