Abstract:
BACKGROUND:The signaling pathway of epithelial to mesenchymal transition (EMT) is regulated by c-Src kinase in many cells. The purpose of this study was to investigate the effects of c-Src kinase on EMT of human lens epithelial cells in vivo stimulated by different factors. METHODS:Human lens epithelial cells, HLE-B3, were exposed to either an inflammatory factor, specifically IL-1α, IL-6, TNF-α or IL-1β, at 10 ng/mL or high glucose (35.5 mM) for 30 mins. Activity of c-Src kinase was evaluated by the expression of p-Src418 with western blot assay. To investigate the effects of activation of c-Src on EMT, HLE-B3 cells were transfected with pCDNA3.1-SrcY530F to upregulate activity of c-Src kinase, and pSlience4.1-ShSrc to knock it down. The expressions of c-Src kinase and molecular markers of EMT such as E-cadherin, ZO-1, α-SMA, and Vimentin were examined at 48 h by RT-PCR and western blot. At 48 h and 72 h of transfection, cell proliferation was detected by MTT, and cell mobility and migration were determined by scratch and transwell assays. RESULTS:Activity of c-Src kinase, which causes the expression of p-Src418, was upregulated by different inflammatory factors and high glucose in HLE-B3 cells. When HLE-B3 cells were transfected with pCDNA3.1-SrcY530F, the expression of c-Src kinase was upregulated on both mRNA and protein levels, and activity of c-Src kinase, expression of p-Src418 increased. The expressions of both E-cadherin and ZO-1 were suppressed, while the expressions of vimentin and α-SMA were elevated on both mRNA and protein levels at the same time. Cell proliferation, mobility and migration increased along with activation of c-Src kinase. Conversely, when HLE-B3 cells were transfected with pSlience4.1-ShSrc, both c-Src kinase and p-Src418 expressions were knocked down. The expressions of E-cadherin and ZO-1 increased, but the expressions of Vimentin and α-SMA decreased; meanwhile, cell proliferation, mobility and migration reduced. CONCLUSIONS:The c-Src kinase in lens epithelial cells is easily activated by external stimuli, resulting in the induction of cell proliferation, mobility, migration and EMT.
journal_name
BMC Ophthalmoljournal_title
BMC ophthalmologyauthors
Li X,Wang F,Ren M,Du M,Zhou Jdoi
10.1186/s12886-019-1229-4subject
Has Abstractpub_date
2019-11-08 00:00:00pages
219issue
1issn
1471-2415pii
10.1186/s12886-019-1229-4journal_volume
19pub_type
杂志文章abstract:BACKGROUND:To evaluate the safety and effectiveness of the Visian Implantable Collamer Lens (ICL) implantation in high myopic patients with inactive Graves' ophthalmopathy (GO) by observing the changes of choroidal thickness (CT). METHODS:Eight patients (16 eyes) with high myopia accompanied with inactive GO were sele...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-020-01612-9
更新日期:2020-08-25 00:00:00
abstract:BACKGROUND:This is the third reported case of a steatocystoma simplex in the lacrimal caruncle. CASE PRESENTATION:A 60-year-old male presented with a history of a slowly progressing mass in the right lacrimal caruncle since several years before his initial visit. At the first examination, a yellowish, relatively smoot...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-016-0358-2
更新日期:2016-10-19 00:00:00
abstract:BACKGROUND:The present study sought to introduce clinical characteristics and stepwise surgical strategies of isolated complete cryptophthalmos, a rare, congenital ocular anomaly. CASE PRESENTATION:Retrospective, noncomparative, clinical study. Six patients with isolated complete cryptophthalmos were diagnosed at the ...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-019-1170-6
更新日期:2019-07-31 00:00:00
abstract:BACKGROUND:To report a case of disseminated fusariosis with endogenous endophthalmitis in a patient with acute lymphoblastic leukemia. Transfusion-associated immune modulation secondary to platelet transfusion could play an important role in the pathophysiology of this case. CASE PRESENTATION:A 9 year-old male with ac...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/1471-2415-11-30
更新日期:2011-11-02 00:00:00
abstract:BACKGROUND:To evaluate the differences between the predicted and achieved lenticule thickness (ΔLT) after small incision lenticule extraction (SMILE) surgery and investigate relationships between ΔLT and predicted lenticule thickness in SMILE. METHODS:A total of 184 eyes from 184 consecutive patients who underwent SMI...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-020-01374-4
更新日期:2020-03-17 00:00:00
abstract:BACKGROUND:The Heimann-Bielschowsky phenomenon (HBP) is an underrecognized condition characterized by slow, pendular, vertical oscillations of the eye accompanying monocular vision loss. Hypotropic dissociated vertical deviation (DVD) is another rare condition induced by asymmetric visual input. This report documents a...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-020-01502-0
更新日期:2020-06-26 00:00:00
abstract:BACKGROUND:Idiopathic orbital pseudotumour is rare in children. We report a case of bilateral paediatric idiopathic orbital pseudotumour and review the characteristics of this case. CASE PRESENTATION:A 14-year-old female patient presented at our Department of Pulmonary and Critical Care Medicine (PCCM) with complaints...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-020-01718-0
更新日期:2020-11-16 00:00:00
abstract:BACKGROUND:To evaluate the changes of choroidal vascular structures in patients after phacoemulsification surgery. METHODS:A self-control study was conducted on 36 eyes of 36 patients who had uneventful phacoemulsification. Choroidal images were acquired preoperatively, 7 days (D7), 1 month (M1), and 3 months (M3) aft...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-018-0749-7
更新日期:2018-03-23 00:00:00
abstract:BACKGROUND:Primary lacrimal sac melanoma is an extremely rare condition with fewer than 50 cases reported so far. Clinically, its symptoms resemble those of dacryocystitis, leading to frequent misdiagnosis. During diagnosis, imaging examination is often performed to differentiate tumors from inflammation. In this repor...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-020-01356-6
更新日期:2020-05-14 00:00:00
abstract:BACKGROUND:To determine the accuracy of the T2 formula as applied to highly myopic eyes, to compare the T2 formula to the SRK/T and Holladay 1 formulas, and to describe possible ways to improve the estimation of corneal height and prediction error in two settings, the Hadassah Hospital, Ophthalmology Department, Jerusa...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-019-1226-7
更新日期:2019-11-12 00:00:00
abstract:BACKGROUND:Endogenous endophthalmitis could lead to a devastating outcome without a prompt and appropriate management. We report a case of advanced endogenous Klebsiella pneumoniae endophthalmitis with extensive subretinal abscess that was successfully treated with a vitrectomy. CASE PRESENTATION:A systemically well 6...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-018-0908-x
更新日期:2018-09-05 00:00:00
abstract:BACKGROUND:Artificial iris anterior chamber implants were originally developed for therapeutic purposes but have been used recently for the cosmetic alteration of eye colour. There is a growing body of evidence surrounding their associated risks. We report a case presenting with complications secondary to bilateral New...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-015-0084-1
更新日期:2015-08-08 00:00:00
abstract:BACKGROUND:The use of capsular tension ring (CTR) implantation to treat cataract patients with weak zonules is still controversial. The aim of this study was to examine the effects of CTR implantation on capsular stability after phacoemulsification in patients with weak zonules, especially patients who have undergone p...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-020-01772-8
更新日期:2021-01-07 00:00:00
abstract:BACKGROUND:Because latanoprost and the original formulation of travoprost that included benzalkonium chloride (BAK) have been shown to be similar with regard to tolerability, we compared initial topical intraocular pressure (IOP)-lowering medication change rates in patients newly treated with latanoprost or travoprost-...
journal_title:BMC ophthalmology
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2415-11-13
更新日期:2011-06-13 00:00:00
abstract:BACKGROUND:Central corneal thickness (CCT) measurement has become an important test in the diagnosis and management of glaucoma. Currently, ultrasound corneal thickness measurement (pachymetry) is the most frequently used clinical technique and the gold standard to assess CCT. Newer instruments are currently available ...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-015-0116-x
更新日期:2015-09-29 00:00:00
abstract:BACKGROUND:To study the influencing factors for different healing patterns of patients with idiopathic macular holes (IMH) after vitrectomy surgery performed with the internal limiting membrane (ILM) flap technique. METHODS:This study was a retrospective, consecutive, observational case series study. We recruited 52 I...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-019-1265-0
更新日期:2019-12-21 00:00:00
abstract:BACKGROUND:Epidemic keratoconjunctivitis (EKC) is one of the most common eye infections worldwide. The analysis of clinical manifestations in different age groups help better know the disease. This study aims to provide more detailed analysis of 272 cases of EKC in 8 years, describe the differences of the clinical feat...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-019-1266-z
更新日期:2019-12-19 00:00:00
abstract:BACKGROUND:To study the healing processes of partial thickness wounds in the adult rabbit cornea after grafting a porous collagen-glycosaminoglycan copolymer matrix (CG). METHODS:In this study, the regeneration of surgically-induced rabbit corneal defect implanted with CG was investigated. The corneal partial thicknes...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-018-0947-3
更新日期:2018-10-25 00:00:00
abstract:BACKGROUND:With the diabetes mellitus (DM) prevalence increasing annually, the human grading of retinal images to evaluate DR has posed a substantial burden worldwide. SmartEye is a recently developed fundus image processing and analysis system with lesion quantification function for DR screening. It is sensitive to th...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-019-1196-9
更新日期:2019-08-14 00:00:00
abstract:BACKGROUND:To assess the reliability of the measurements obtained with the PalmScan, when compared with another standardized A-mode ultrasound device, and assess the consistency and correlation between the two methods. METHODS:Transversal, descriptive, and comparative study. We recorded the axial length (AL), anterior...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/1471-2415-10-8
更新日期:2010-03-24 00:00:00
abstract:BACKGROUND:Investigating the efficacy and safety of phacoemulsification with intraocular lens (IOL) implantation in corneal opacities. METHODS:This retrospective study was conducted in a tertiary medical center. Twenty-three eyes of 19 patients with cataracts and corneal opacities obscuring the pupillary center having...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-018-0765-7
更新日期:2018-04-23 00:00:00
abstract:BACKGROUND:Retinal artery occlusion caused by myxoma is relatively rare. There are several points that should be taken into consideration to avoid overlooking this disorder. CASE PRESENTATION:This case report describes a 43-year-old woman with sudden vision loss in her left eye for 20 days after single sudden syncope....
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/1471-2415-14-164
更新日期:2014-12-23 00:00:00
abstract:BACKGROUND:To present the follow-up outcomes of a modified technique of transscleral suture fixation of posterior chamber intraocular lens (PCIOL) in eyes with inadequate capsule support. METHODS:A retrospective chart review of 21 patients underwent transscleral suture fixation of a foldable 3-looped haptics one-piece...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-019-1113-2
更新日期:2019-05-09 00:00:00
abstract:BACKGROUND:This study sought to identify factors associated with retinal detachment and retreatment of aggressive posterior retinopathy of prematurity (APROP) initially treated with intravitreal ranibizumab (IVR) injection as well as the efficacy of IVR treatment. METHODS:This was a retrospective study. A total of 83 ...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-018-0815-1
更新日期:2018-06-26 00:00:00
abstract:BACKGROUND:Acute Posterior Multifocal Placoid Pigment Epitheliopathy (APMPPE) is a rare inflammatory eye disease that affects the Retinal Pigment Epithelium and outer retina. The purpose of this study was to describe its presentations, as well as its prognosis in a series of untreated patients. METHODS:Records of pati...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-018-0744-z
更新日期:2018-03-20 00:00:00
abstract:BACKGROUND:To evaluate the relationships between Reichert Ocular Response Analyzer (ORA) parameters corneal hysteresis (CH) and corneal response factor (CRF) and ocular dimensions, age and intraocular pressure. METHODS:Two hundred and twelve eyes of 212 participants with no ocular pathology had CH and CRF measured wit...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/1471-2415-14-11
更新日期:2014-01-30 00:00:00
abstract:BACKGROUND:Goldenhar Syndrome is characterized by malformations of multiple anatomical districts. Between these, bulbar dermoids are common and represent a significant clinical problem as they can affect both ocular function and aesthetic comfort.Histologic characterization of dermoids has been extensively performed; h...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/1471-2415-13-55
更新日期:2013-10-16 00:00:00
abstract:BACKGROUND:Lateralization of horizontal semicircular canal benign paroxysmal positional vertigo (HSC-BPPV) is very important for successful repositioning. The directions of lying-down nystagmus (LDN) and head-bending nystagmus (HBN) have been used as ancillary findings to identify the affected sites. This retrospective...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/1471-2415-14-136
更新日期:2014-11-20 00:00:00
abstract:BACKGROUND:To study the value and safety of aqueous humor polymerase chain reaction (PCR) analysis for Herpes simplex, varicella zoster, cytomegalovirus, Epstein-Barr virus and Toxoplasma gondii in patients with uveitis. METHODS:Records of 45 consecutive patients with anterior and posterior uveitis who underwent AC pa...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-016-0369-z
更新日期:2016-10-28 00:00:00
abstract:BACKGROUND:Fat embolism in the deep retinal capillary plexus is one of the reported mechanisms underlying central/paracentral scotoma in patients with Purtscher's retinopathy. Here we report the clear delineation of capillary dropout in the deep capillary plexus using optical coherence tomography angiography (OCTA) in ...
journal_title:BMC ophthalmology
pub_type: 杂志文章
doi:10.1186/s12886-016-0298-x
更新日期:2016-07-19 00:00:00