NEAT1/miR-23a-3p/KLF3: a novel regulatory axis in melanoma cancer progression.


Background:Melanoma is an extremely aggressive malignant skin tumor with high mortality. Many types of long noncoding RNAs and microRNAs have been reported to be associated with the oncogenesis of melanoma. However, a novel lncRNA-NEAT has not been thoroughly investigated in melanoma cancer. The purposes of this study were to investigate the underlying molecular mechanism in a novel couple of lnc-NEAT1 and miR-23a-3p, as well as the function role of KLF3 in the regulation of melanoma cancer. Methods:28 groups of tumor tissues and normal tissues were obtained from melanoma cancer patients. We performed a series of experiments and analysis, including RT-qPCR, western blots, CCK-8 assay, and migration/invasion assay, to investigate the expressions of NEAT1, miR-23a-5p and KLF3, cell viabilities, and tumor growth in vivo. Results:In this study, we observed that the expression of NEAT1 was significantly upregulated in melanoma tissues, which remarkedly promoted the cells' proliferation, cell migration, and invasion in melanoma cell lines. Besides, NEAT1 could directly bind to miR-23a-3p, which was found to reverse the effect caused by NEAT1. MiR-23a-3p was discovered to bind to 3'UTR of KLF3, which reduced KLF3 expression. In addition, the overexpression of KLF3 could lower the effects of miR-23a-3p caused on melanoma cancer cell development. Conclusion:Our results demonstrated that NEAT1 could sponge miR-23a-3p and functions via the expression of KLF3. This axis of NEAT1/miR-23a-5p/KLF3 could together regulate melanoma cancer proliferation. This might provide a new therapeutic strategy for melanoma skin cancer.Trial registration HBTCM38574839, registered 12 October 2012.


Cancer Cell Int


Ding F,Lai J,Gao Y,Wang G,Shang J,Zhang D,Zheng S




Has Abstract


2019-08-22 00:00:00










  • Association between underexpression of microrna-203 and clinicopathological significance in hepatocellular carcinoma tissues.

    abstract:BACKGROUND:Although recent studies have shown the utility of miR-203 as a cancer-relevant biomarker, the validated clinical significance of miR-203 in HCC remains obscure. The aim of the present study was to evaluate the relationship between miR-203 expression and clinicopathological features in HCC patients. METHODS:...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Liu Y,Ren F,Rong M,Luo Y,Dang Y,Chen G

    更新日期:2015-06-18 00:00:00

  • Observations on the expression of human papillomavirus major capsid protein in HeLa cells.

    abstract:BACKGROUND:The goal of this study was to identify the nature of the inclusion bodies that have been found in HeLa cells (cervical cancer immortal cell line) by electron microscope and to determine whether the major capsid protein (L1) of human papillomavirus (HPV) can be expressed in HPV-positive uterine cervix cancer ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Xiao CY,Fu BB,Li ZY,Mushtaq G,Kamal MA,Li JH,Tang GC,Xiao SS

    更新日期:2015-05-23 00:00:00

  • Bioinformatics combined with quantitative proteomics analyses and identification of potential biomarkers in cholangiocarcinoma.

    abstract:Background:Cholangiocarcinoma (CCA) is an invasive malignancy arising from biliary epithelial cells; it is the most common primary tumour of the bile tract and has a poor prognosis. The aim of this study was to screen prognostic biomarkers for CCA by integrated multiomics analysis. Methods:The GSE32225 dataset was der...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Da Z,Gao L,Su G,Yao J,Fu W,Zhang J,Zhang X,Pei Z,Yue P,Bai B,Lin Y,Meng W,Li X

    更新日期:2020-04-22 00:00:00

  • Combined high expression of CD47 and CD68 is a novel prognostic factor for breast cancer patients.

    abstract:Background:Avoiding the phagocytosis by tumor-associated macrophages (TAMs) is necessary for the growth and metastasis of solid tumors. CD47 binds to the receptor signal-regulatory protein-α (SIRP-α) on the macrophages to avoid normal phagocytosis. In this study, we evaluated the expression and prognostic significance ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Yuan J,He H,Chen C,Wu J,Rao J,Yan H

    更新日期:2019-09-11 00:00:00

  • MiR-20a-5p suppresses tumor proliferation by targeting autophagy-related gene 7 in neuroblastoma.

    abstract:Background:Neuroblastoma (NB) is the most common malignant tumor originating from the extracranial sympathetic nervous system in children. The molecular mechanisms underlying this disease are complex, and not completely understood. Methods:Quantitative real-time PCR (qRT-PCR) was applied to quantify the expression of ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Yu Y,Zhang J,Jin Y,Yang Y,Shi J,Chen F,Han S,Chu P,Lu J,Wang H,Guo Y,Ni X

    更新日期:2018-01-04 00:00:00

  • microRNA-365 inhibits YAP through TLR4-mediated IRF3 phosphorylation and thereby alleviates gastric precancerous lesions.

    abstract:BACKGROUND:Gastric carcinoma (GC) is currently one of the most common malignant tumors of the digestive system, and gastric precancerous lesions play a vital role in studying the mechanism of GC. Multiple microRNAs (miRNAs) have been documented to be potential biomarkers to indicate progression of gastric precancerous ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhang T,Zhang K,Ji K,Zhang C,Jiang Y,Zhang Q,Tian Z,Wang X,Zhang M,Li X

    更新日期:2020-11-13 00:00:00

  • Evaluation of antiproliferative and anti-inflammatory activities of methanol extract and its fractions from the Mediterranean sponge.

    abstract:BACKGROUND:Without doubt, natural products have been, and still are, the cornerstone of the health care armamentarium. Of all natural sources, the marine environment is clearly the last great frontier for pharmaceutical and medical research. METHODS:This work progresses in the direction of identifying component(s) fro...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Dellai A,Deghrigue M,Laroche-Clary A,Masour HB,Chouchane N,Robert J,Bouraoui A

    更新日期:2012-05-15 00:00:00

  • TMPO-AS1 promotes cell proliferation of thyroid cancer via sponging miR-498 to modulate TMPO.

    abstract:Background:Thyroid cancer (TC) is the most frequent endocrine malignancy. Long noncoding RNAs (lncRNAs) have been confirmed to act as significant roles in tumor development. The role of lncRNA TMPO-AS1 in TC is still unclear, so it remains to be explored. The aim of the research is to investigate the role and regulator...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Li Z,Feng Y,Zhang Z,Cao X,Lu X

    更新日期:2020-07-08 00:00:00

  • Alantolactone inhibits cell autophagy and promotes apoptosis via AP2M1 in acute lymphoblastic leukemia.

    abstract:Background:Acute lymphoblastic leukemia (ALL) is an aggressive hematopoietic malignancy that is most commonly observed in children. Alantolactone (ALT) has been reported to exhibit anti-tumor activity in different types of cancer. The aim of the present study was to investigate the anti-tumor activity and molecular mec...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Shi C,Lan W,Wang Z,Yang D,Wei J,Liu Z,Teng Y,Gu M,Yuan T,Cao F,Zhou J,Li Y

    更新日期:2020-09-09 00:00:00

  • A signature of hypoxia-related factors reveals functional dysregulation and robustly predicts clinical outcomes in stage I/II colorectal cancer patients.

    abstract:Background:The hypoxic tumor microenvironment accelerates the invasion and migration of colorectal cancer (CRC) cells. The aim of this study was to develop and validate a hypoxia gene signature for predicting the outcome in stage I/II CRC patients that have limited therapeutic options. Methods:The hypoxic gene signatu...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zou YF,Rong YM,Tan YX,Xiao J,Yu ZL,Chen YF,Ke J,Li CH,Chen X,Wu XJ,Lan P,Lin XT,Gao F

    更新日期:2019-09-23 00:00:00

  • Correction to: ZEB1‑AS1 initiates a miRNA‑mediated ceRNA network to facilitate gastric cancer progression.

    abstract::[This corrects the article DOI: 10.1186/s12935-019-0742-0.]. ...

    journal_title:Cancer cell international

    pub_type: 杂志文章,已发布勘误


    authors: Ma MH,An JX,Zhang C,Liu J,Liang Y,Zhang CD,Zhang Z,Dai DQ

    更新日期:2019-04-11 00:00:00

  • Ibrutinib in B-cell lymphoma: single fighter might be enough?

    abstract:Background:In recent years, the B cell receptor (BCR) signaling pathway has become a "hot point" because it plays a critical role in B-cell proliferation and function. Bruton's tyrosine kinase (BTK) is overexpressed in many subtypes of B-cell lymphoma as a downstream kinase in the BCR signaling pathway. Ibrutinib, the ...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审


    authors: Xue C,Wang X,Zhang L,Qu Q,Zhang Q,Jiang Y

    更新日期:2020-09-29 00:00:00

  • Human urothelial carcinoma cell response to Sunitinib malate therapy in vitro.

    abstract:OBJECTIVES:Bladder transitional cell carcinoma (TCC) is one of the most common solid malignancies in China. This study examined the antitumor effect and underlying mechanism of action of sunitinib malate in human bladder TCC in vitro. METHODS:Bladder TCC cell lines 5637 and BIU87 were maintained in 1640 medium and T24...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Wen J,Li HZ,Ji ZG,Jin J

    更新日期:2015-02-28 00:00:00

  • The novel mTOR inhibitor CCI-779 (temsirolimus) induces antiproliferative effects through inhibition of mTOR in Bel-7402 liver cancer cells.

    abstract:BACKGROUND:Liver cancer is one of the most frequent cancers in the world. Targeted therapy of cancer with specific inhibitors is developing and has shown promising antitumor efficacy. CCI-779 (temsirolimus), a specific inhibitor of mTOR (mammalian target of rapamycin), can block the mTOR signaling pathway. Here, we sys...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Li S,Liang Y,Wu M,Wang X,Fu H,Chen Y,Wang Z

    更新日期:2013-03-28 00:00:00

  • Inhibition of androgen-independent prostate cancer cell growth is enhanced by combination therapy targeting Hedgehog and ErbB signalling.

    abstract:BACKGROUND:Prostate cancer is a leading cause of male cancer specific mortality. When cure by radical prostatectomy is not possible the next line of prostate cancer treatment is androgen deprivation. However prolonged androgen deprivation often results in relapse and androgen-independent prostate cancer that is inevita...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Shaw G,Prowse DM

    更新日期:2008-03-18 00:00:00

  • The effect of tetrandrine combined with cisplatin on proliferation and apoptosis of A549/DDP cells and A549 cells.

    abstract:BACKGROUND:Non-small cell lung cancer comprises the majority of lung cancer cases and is insensitive to chemotherapy. Most patients develop drug resistance. Recently, tetrandrine (TET), a bis-benzylisoquinoline alkaloid, was identified as a novel anti-cancer agent. However, the effect of tetrandrine combined with cispl...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Ye LY,Hu S,Xu HE,Xu RR,Kong H,Zeng XN,Xie WP,Wang H

    更新日期:2017-03-27 00:00:00

  • Downregulation of long non-coding RNA MAFG-AS1 represses tumorigenesis of colorectal cancer cells through the microRNA-149-3p-dependent inhibition of HOXB8.

    abstract:Background:Colorectal cancer (CRC) is considered as the second common death-induced cancer. More recently, association of long non-coding RNAs (lncRNAs) with CRC has been extensively investigated. Therefore, the present study was performed to determine whether lncRNA MAF BZIP Transcription Factor G Antisense RNA 1 (MAF...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Ruan Z,Deng H,Liang M,Xu Z,Lai M,Ren H,Deng X,Su X

    更新日期:2020-10-19 00:00:00

  • Identification and validation of genes involved in gastric tumorigenesis.

    abstract:BACKGROUND:Gastric cancer is one of the common cancers seen in south India. Unfortunately more than 90% are advanced by the time they report to a tertiary centre in the country. There is an urgent need to characterize these cancers and try to identify potential biomarkers and novel therapeutic targets. MATERIALS AND M...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Rajkumar T,Vijayalakshmi N,Gopal G,Sabitha K,Shirley S,Raja UM,Ramakrishnan SA

    更新日期:2010-11-24 00:00:00

  • Avenanthramide-C reduces the viability of MDA-MB-231 breast cancer cells through an apoptotic mechanism.

    abstract:BACKGROUND:Avenanthramides (AVN) are a relatively unstudied family of phytochemicals that could be novel chemotherapeutics. These compounds, found in oats, are non-toxic to healthy cells and have been shown to reduce viability of human colon and liver cancers in vitro. However, these studies do not elucidate a molecula...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Hastings J,Kenealey J

    更新日期:2017-10-18 00:00:00

  • p62 acts as an oncogene and is targeted by miR-124-3p in glioma.

    abstract:Background:Glioma is the most common central nervous system (CNS) tumour. p62, an important autophagy adaptor, plays a crucial role in cancer. However, the role of p62 in the progression of glioma is poorly characterized. Methods:We examined the expression of p62 in glioma tissues and cell lines. Then we investigated ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Deng D,Luo K,Liu H,Nie X,Xue L,Wang R,Xu Y,Cui J,Shao N,Zhi F

    更新日期:2019-11-06 00:00:00

  • Prognostic nutritional index and the prognosis of diffuse large b-cell lymphoma: a meta-analysis.

    abstract:Background:Some studies have investigated the prognostic value exhibited by the Prognostic Nutritional Index (PNI) in patients suffering diffuse large B-cell lymphoma (DLBCL), but varying results were obtained. In order to determine the specific prognostic value more accurately, a meta-analysis was conducted in this st...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审


    authors: Luan C,Wang F,Wei N,Chen B

    更新日期:2020-09-15 00:00:00

  • Silencing lncRNA ZFAS1 or elevated microRNA-135a represses proliferation, migration, invasion and resistance to apoptosis of osteosarcoma cells.

    abstract:Background:Osteosarcoma (OS) is still a disease with high mortality from malignant tumors in children and adolescents. Due to its poor treatment, this study explored the involvement of lncRNA ZFAS1/microRNA-135a (miR-135a)/apurinic/apyrimidinic exonuclease 1 (APEX1) axis in the regulation of OS growth and metastasis. ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhao Z,Lin X,Tong Y,Li W

    更新日期:2019-12-03 00:00:00

  • Association of TNFAIP8 gene polymorphisms with endometrial cancer in northern Chinese women.

    abstract:Background:Tumor necrosis factor-a-induced protein 8 (TNFAIP8) presented a elevated expression in endometrial cancer (EC). However, the relationship of TNFAIP8 gene polymorphisms with EC risk remains unclear. This case-control study aimed to investigate the effect of single nucleotide polymorphisms (SNPs) in TNFAIP8 on...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Liu T,Jiang L,Yu L,Ge T,Wang J,Gao H

    更新日期:2019-04-23 00:00:00

  • Breast cancer pathogenesis is linked to the intra-tumoral estrogen sulfotransferase (hSULT1E1) expressions regulated by cellular redox dependent Nrf-2/NFκβ interplay.

    abstract:Background:Estrogen sulfotransferase catalyzes conjugation of sulfuryl-group to estradiol/estrone and regulates E2 availability/activity via estrogen-receptor or non-receptor mediated pathways. Sulfoconjugated estrogen fails to bind estrogen-receptor (ER). High estrogen is a known carcinogen in postmenopausal women. Re...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Nazmeen A,Chen G,Ghosh TK,Maiti S

    更新日期:2020-03-04 00:00:00

  • MiR-145-5p suppresses the proliferation, migration and invasion of gastric cancer epithelial cells via the ANGPT2/NOD_LIKE_RECEPTOR axis.

    abstract:Objective:This study aimed to investigate the relationship among miR-145-5p, ANGPT2 and the NOD_LIKE_RECEPTOR pathway, thereby revealing the molecular mechanism of these three factors underlying the proliferation, migration and invasion of gastric cancer (GC) epithelial cells. Methods:qRT-PCR was carried out to detect...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhou K,Song B,Wei M,Fang J,Xu Y

    更新日期:2020-08-28 00:00:00

  • Overexpression of microRNA-29b inhibits epithelial-mesenchymal transition and angiogenesis of colorectal cancer through the ETV4/ERK/EGFR axis.

    abstract:BACKGROUND:Recent studies have reported the involvement of microRNA-29 (miR-29) family members in human cancers through their ability to regulate cellular functions. The present study investigated biological function of miR-29b in colorectal cancer (CRC). METHODS:CRC tissues and adjacent normal tissues were collected ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Leng Y,Chen Z,Ding H,Zhao X,Qin L,Pan Y

    更新日期:2021-01-06 00:00:00

  • Human cytomegalovirus interleukin-10 enhances matrigel invasion of MDA-MB-231 breast cancer cells.

    abstract:BACKGROUND:While some risk factors for breast cancer are well-known, the influence of other factors, particularly virus infection, remains unclear. Human cytomegalovirus (HCMV) is widespread in the general population, and both molecular and epidemiological evidence has indicated links between HCMV and breast cancer. Th...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Valle Oseguera CA,Spencer JV

    更新日期:2017-02-13 00:00:00

  • HIF-2alpha downregulation in the absence of functional VHL is not sufficient for renal cell differentiation.

    abstract:BACKGROUND:Mutational inactivation of the von Hippel-Lindau (VHL) tumor suppressor gene has been linked to hereditary as well as sporadic clear cell renal carcinomas. The product of the VHL gene, pVHL, acts to target hypoxia-inducible factor alpha (HIF-alpha) subunits for ubiquitination and subsequent degradation. Usin...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Hughes MD,Kapllani E,Alexander AE,Burk RD,Schoenfeld AR

    更新日期:2007-06-28 00:00:00

  • Differential effects of HIF-α isoforms on apoptosis in renal carcinoma cell lines.

    abstract:BACKGROUND:Germline mutations in the von Hippel-Lindau (VHL) tumor suppressor gene predispose individuals to clear cell renal carcinomas, hemangioblastomas, and pheochromocytomas. The VHL gene product forms an ubiquitin E3 ligase complex, with regulation of hypoxia-inducible factor alpha (HIF-α) as its best known funct...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Doonachar A,Gallo MD,Doukas D,Pasricha R,Lantsberg I,Schoenfeld AR

    更新日期:2015-02-22 00:00:00

  • Transgelin interacts with PARP1 in human colon cancer cells.

    abstract:Background:Transgelin, an actin-binding protein, is associated with cytoskeleton remodeling. Findings from our previous studies demonstrated that transgelin was up-regulated in node-positive colorectal cancer (CRC) versus node-negative disease. Over-expression of TAGLN affected the expression of 256 downstream transcri...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Lew ZX,Zhou HM,Fang YY,Ye Z,Zhong W,Yang XY,Yu Z,Chen DY,Luo SM,Chen LF,Lin Y

    更新日期:2020-08-03 00:00:00