Abstract：
Background:Melanoma is an extremely aggressive malignant skin tumor with high mortality. Many types of long noncoding RNAs and microRNAs have been reported to be associated with the oncogenesis of melanoma. However, a novel lncRNA-NEAT has not been thoroughly investigated in melanoma cancer. The purposes of this study were to investigate the underlying molecular mechanism in a novel couple of lnc-NEAT1 and miR-23a-3p, as well as the function role of KLF3 in the regulation of melanoma cancer. Methods:28 groups of tumor tissues and normal tissues were obtained from melanoma cancer patients. We performed a series of experiments and analysis, including RT-qPCR, western blots, CCK-8 assay, and migration/invasion assay, to investigate the expressions of NEAT1, miR-23a-5p and KLF3, cell viabilities, and tumor growth in vivo. Results:In this study, we observed that the expression of NEAT1 was significantly upregulated in melanoma tissues, which remarkedly promoted the cells' proliferation, cell migration, and invasion in melanoma cell lines. Besides, NEAT1 could directly bind to miR-23a-3p, which was found to reverse the effect caused by NEAT1. MiR-23a-3p was discovered to bind to 3'UTR of KLF3, which reduced KLF3 expression. In addition, the overexpression of KLF3 could lower the effects of miR-23a-3p caused on melanoma cancer cell development. Conclusion:Our results demonstrated that NEAT1 could sponge miR-23a-3p and functions via the expression of KLF3. This axis of NEAT1/miR-23a-5p/KLF3 could together regulate melanoma cancer proliferation. This might provide a new therapeutic strategy for melanoma skin cancer.Trial registration HBTCM38574839, registered 12 October 2012.
journal_name
Cancer Cell Intjournal_title
Cancer cell internationalauthors
Ding F,Lai J,Gao Y,Wang G,Shang J,Zhang D,Zheng Sdoi
10.1186/s12935-019-0927-6subject
Has Abstractpub_date
2019-08-22 00:00:00pages
217issn
1475-2867pii
927journal_volume
19pub_type
杂志文章abstract：Background:Immunotherapies that targeting programmed cell death 1 (PD-1) and programmed death-ligand 1 (PD-L1) have obtained prominent success in breast cancer (BC). However, not all the patients benefit from the antibody therapy. This study aimed to identify PD-1/PD-L1 correlated genes and pathways as well as investig...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0955-2
更新日期：2019-09-09 00:00:00
abstract：Objective:This study aimed to investigate the relationship among miR-145-5p, ANGPT2 and the NOD_LIKE_RECEPTOR pathway, thereby revealing the molecular mechanism of these three factors underlying the proliferation, migration and invasion of gastric cancer (GC) epithelial cells. Methods:qRT-PCR was carried out to detect...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01483-6
更新日期：2020-08-28 00:00:00
abstract：Background:Estrogen sulfotransferase catalyzes conjugation of sulfuryl-group to estradiol/estrone and regulates E2 availability/activity via estrogen-receptor or non-receptor mediated pathways. Sulfoconjugated estrogen fails to bind estrogen-receptor (ER). High estrogen is a known carcinogen in postmenopausal women. Re...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-1153-y
更新日期：2020-03-04 00:00:00
abstract：Background:Acute lymphoblastic leukemia (ALL) is an aggressive hematopoietic malignancy that is most commonly observed in children. Alantolactone (ALT) has been reported to exhibit anti-tumor activity in different types of cancer. The aim of the present study was to investigate the anti-tumor activity and molecular mec...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01537-9
更新日期：2020-09-09 00:00:00
abstract：Background:Dysregulation of long non-coding RNAs (lncRNAs) results in development of human diseases including hepatocellular carcinoma (HCC). Although several HCC related lncRNAs have been reported, the biological functions of many lncRNAs during the development of HCC remains unknown. Methods:The expression of ST8SIA...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01325-5
更新日期：2020-06-11 00:00:00
abstract：Background:Esophageal squamous cell carcinoma (ESCC) is one of the most prevalent malignancies and a major cause of cancer related death worldwide, especially in China. Cell lines are widely used disease models for basic medical research, however, well characterized ESCC cell models from China were seldom reported. Mis...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01268-x
更新日期：2020-05-24 00:00:00
abstract：Background:MicroRNAs (miRNAs) play key roles in tumorigenesis and progression of gastric cancer (GC). miR-1269 has been reported to be upregulated in several cancers and plays a crucial role in carcinogenesis and cancer progression. However, the biological function of miR-1269 in human GC and its mechanism remain uncle...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-1026-4
更新日期：2019-11-21 00:00:00
abstract：BACKGROUND:Resistance to drug therapy is a major impediment for successful treatment of patients suffering from breast cancer (BC). Tamoxifen (TAM) is an extensively used therapeutic agent, which substantially reduces the risk of recurrence and associated mortality in BC. This study demonstrated that exosomal transfer ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01659-0
更新日期：2021-01-15 00:00:00
abstract：Background:Programmed cell death 1 (PD-1) functions as an immune checkpoint in the process of anti-tumor immune response. The PD-1 blockade is now becoming a fundamental part in cancer immunotherapy. So it's essential to elicit the PD-1 related immune process in different types of cancer. Methods:The Cancer Genome Atl...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-018-0712-y
更新日期：2018-12-27 00:00:00
abstract：Background:At the present time, colorectal cancer (CRC) is still known as a disease with a high mortality rate. Theranostics are flawless scenarios that link diagnosis with therapy, including precision medicine as a critical platform that relies on the development of biomarkers particularly "liquid biopsy". Circulating...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-020-01389-3
更新日期：2020-07-06 00:00:00
abstract：Background:Endometrial cancer (EC) is the fourth most common malignancy of the female genital tract worldwide. MicroRNAs are important gene regulators with critical roles in diverse biological processes, including tumorigenesis. Several study's show that miR-139-5p is involved in the tumorigenesis and metastasis of var...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-018-0545-8
更新日期：2018-04-02 00:00:00
abstract：Background:Newly identified lncRNA zinc finger protein, FOG family member 2 antisense RNA 1 (ZFPM2-AS1) is identified as an oncogenic gene. However, the role of ZFPM2-AS1 in small cell lung cancer (SCLC) is poorly comprehended. Methods:The expression of genes in SCLC tissues and cells was measured by qRT-PCR. Colony f...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-1157-7
更新日期：2020-04-03 00:00:00
abstract：Background and aims:microRNAs (miRNAs) have been reported to regulate proliferation and migration by down-regulating the expression of target genes. The aims of this study were to investigate whether miR-4316 inhibited proliferation and migration by downregulating vascular endothelial growth factor A (VEGF-A) and its c...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-1132-3
更新日期：2020-02-22 00:00:00
abstract：Background:Drug resistance to 5-fluorouracil (5-FU) and recurrence after chemotherapy in colorectal cancer remain a challenge to be resolved for the improvement of patient outcomes. It is recognized that a variety of secretory proteins released from the tumor cells exposed to chemo-drugs into the tumor microenvironment...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01541-z
更新日期：2020-09-14 00:00:00
abstract：:miRNAs, a major class of small noncoding RNAs approximately 18-25 nucleotides in length, function by repressing the expression of target genes through binding to complementary sequences in the 3'-UTRs of target genes. Emerging evidence has highlighted their important roles in numerous diseases, including human cancers...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-019-0984-x
更新日期：2019-10-07 00:00:00
abstract：Background:Cancer cell viability is strongly modulated by the Hippo pathway, which includes mammalian STE20-like protein kinase 1 (Mst1) and yes-associated protein (Yap). Although the roles of Mst1 and Yap in thyroid carcinoma cell death have been fully addressed, no study has determined whether differential modificati...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0860-8
更新日期：2019-05-22 00:00:00
abstract：Background:Accumulating studies showed that long noncoding RNAs (lncRNAs) played vital roles in cancer progression. LncRNA MIR4435-2HG was proved to act as an oncogene in various tumors. However, the underlying function of MIR4435-2HG in ovarian cancer (OC) remains unclear. Methods:The expression levels of MIR4435-2HG...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01227-6
更新日期：2020-05-01 00:00:00
abstract：Background:Tumor necrosis factor-a-induced protein 8 (TNFAIP8) presented a elevated expression in endometrial cancer (EC). However, the relationship of TNFAIP8 gene polymorphisms with EC risk remains unclear. This case-control study aimed to investigate the effect of single nucleotide polymorphisms (SNPs) in TNFAIP8 on...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0827-9
更新日期：2019-04-23 00:00:00
abstract：:[This corrects the article DOI: 10.1186/s12935-019-0742-0.]. ...
journal_title：Cancer cell international
pub_type： 杂志文章,已发布勘误
doi：10.1186/s12935-019-0797-y
更新日期：2019-04-11 00:00:00
abstract：OBJECTIVES:Bladder transitional cell carcinoma (TCC) is one of the most common solid malignancies in China. This study examined the antitumor effect and underlying mechanism of action of sunitinib malate in human bladder TCC in vitro. METHODS:Bladder TCC cell lines 5637 and BIU87 were maintained in 1640 medium and T24...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-015-0179-z
更新日期：2015-02-28 00:00:00
abstract：Background:Melanoma is the most aggressive type of skin cancer with high mortality rate and poor prognosis. lncRNA MEG3, a tumor suppressor, is closely related to the development of various cancers. However, the role of lncRNA MEG3 in melanoma has seldom been studied. Methods:RT-PCR was used to examine the expressions...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-1087-4
更新日期：2020-01-10 00:00:00
abstract：Background:LncRNAs are key regulators in cancer. The current study explored the role of lncRNA LINC00261 (LINC00261) in lung cancer (LC). Methods:Expression of LINC00261 in LC tissues and cells was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Pearson's Chi square test and Kaplan-Meier anal...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01332-6
更新日期：2020-06-26 00:00:00
abstract：BACKGROUND:While some risk factors for breast cancer are well-known, the influence of other factors, particularly virus infection, remains unclear. Human cytomegalovirus (HCMV) is widespread in the general population, and both molecular and epidemiological evidence has indicated links between HCMV and breast cancer. Th...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-017-0399-5
更新日期：2017-02-13 00:00:00
abstract：Background:Long non-coding RNA (lncRNA) BM742401 is a tumor suppressor in gastric cancer and chronic lymphocytic leukemia. As the promoter and coding region of BM742401 are fully embedded in a CpG island, we hypothesized that BM742401 is a tumor suppressor lncRNA epigenetically silenced by promoter DNA methylation in m...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01504-4
更新日期：2020-08-25 00:00:00
abstract：BACKGROUND:Despite the modern therapies available for treating glioblastoma multiforme (GBM), it is still a deadly disease. The development of new therapeutic strategies for the management of gliomas is therefore crucial. The present study is designed to analyze the therapeutic potentials of synthetic compound N-(2-hyd...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-014-0133-5
更新日期：2014-11-30 00:00:00
abstract：Background:Endometrial carcinoma (EC) is one of the three major malignant tumors of the female reproductive system. In recent years, the incidence and mortality rate of EC have increased. B-cell translocation gene 1 (BTG1) is an anti-proliferation gene that regulates the occurrence and development of a variety of tumor...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01591-3
更新日期：2020-10-07 00:00:00
abstract：Background:Tumor necrosis factor-α (TNF-α) immunotherapy controls the progression of human cervical cancer. Here, we explored the detailed molecular mechanisms played by melatonin in human cervical cancer (HeLa cells) death in the presence of TNF-α injury, with a particular attention to the mitochondrial homeostasis. ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0777-2
更新日期：2019-03-14 00:00:00
abstract：BACKGROUND:ErbB3-binding protein 1 (Ebp1) is a member of the PA2G4 family of proliferation-regulated proteins that is expressed in multiple malignant and non-malignant cells. ErbB3 and other members of the EGFR family have been implicated in cancer progression, it however remains unknown whether Ebp1 participate in pro...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-8-18
更新日期：2008-11-24 00:00:00
abstract：BACKGROUND:Epithelial mesenchymal transition (EMT) mediated by TGF-β pays an important role in malignant tumor acquired abilities of migration and invasion. Our previous study showed that the extract of Stellera chamaejasme L. (ESC) was against proliferation of a variety of tumor cells, but there were no studies in the...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-015-0265-2
更新日期：2015-12-10 00:00:00
abstract：BACKGROUND:Without doubt, natural products have been, and still are, the cornerstone of the health care armamentarium. Of all natural sources, the marine environment is clearly the last great frontier for pharmaceutical and medical research. METHODS:This work progresses in the direction of identifying component(s) fro...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-12-18
更新日期：2012-05-15 00:00:00
