Abstract：
:Hepatitis C Virus (HCV) remains an important public health threat with approximately 170 million carriers worldwide who are at risk of developing hepatitis C-associated end-stage liver diseases. Despite improvement of HCV treatment using the novel direct-acting antivirals (DAAs) targeting viral replication, there is a lack of prophylactic measures for protection against HCV infection. Identifying novel antivirals such as those that target viral entry could help broaden the therapeutic arsenal against HCV. Herein, we investigated the anti-HCV activity of the methanolic extract from Rhizoma coptidis (RC), a widely used traditional Chinese medicine documented by the WHO and experimentally reported to possess several pharmacological functions including antiviral effects. Using the cell culture-derived HCV system, we demonstrated that RC dose-dependently inhibited HCV infection of Huh-7.5 cells at non-cytotoxic concentrations. In particular, RC blocked HCV attachment and entry/fusion into the host cells without exerting any significant effect on the cell-free viral particles or modulating key host cell entry factors to HCV. Moreover, RC robustly suppressed HCV pseudoparticles infection of Huh-7.5 cells and impeded infection by several HCV genotypes. Collectively, our results identified RC as a potent antagonist to HCV entry with potential pan-genotypic properties, which deserves further evaluation for use as an anti-HCV agent.
journal_name
Virusesjournal_title
Virusesauthors
Hung TC,Jassey A,Lin CJ,Liu CH,Lin CC,Yen MH,Lin LTdoi
10.3390/v10120669subject
Has Abstractpub_date
2018-11-27 00:00:00issue
12issn
1999-4915pii
v10120669journal_volume
10pub_type
杂志文章相关文献
Viruses-Basel文献大全abstract：:Bats are unique mammals, exhibit distinctive life history traits and have unique immunological approaches to suppression of viral diseases upon infection. High-throughput next-generation sequencing has been used in characterizing the virome of different bat species. The cave nectar bat, Eonycteris spelaea, has a broad...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11030250
更新日期：2019-03-12 00:00:00
abstract：:We use a Species Distribution Modeling (SDM) approach along with Geographic Information Systems (GIS) techniques to examine the potential distribution of hantavirus pulmonary syndrome (HPS) caused by Andes virus (ANDV) in southern Argentina and, more precisely, define and estimate the area with the highest infection p...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v6010201
更新日期：2014-01-14 00:00:00
abstract：:Human parechovirus (HPeV), particularly type 3 (HPeV3), is an important cause of sepsis-/meningitis-like illness in young infants. Laboratory records identified a total of ten HPeV-positive cases in Southeastern Australia between January and July 2019. The HPeV present in these cases were typed by Sanger sequencing of...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11100913
更新日期：2019-10-03 00:00:00
abstract：:Unlike other human herpesviruses, human herpesvirus 6A and 6B (HHV-6A/B) infection can lead to integration of the viral genome in human chromosomes. When integration occurs in germinal cells, the integrated HHV-6A/B genome can be transmitted to 50% of descendants. Such individuals, carrying one copy of the HHV-6A/B ge...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v9070160
更新日期：2017-06-26 00:00:00
abstract：:Extensive surveillance in bat populations in response to recent emerging diseases has revealed that this group of mammals acts as a reservoir for a large range of viruses. However, the oldest known association between a zoonotic virus and a bat is that between rabies virus and the vampire bat. Vampire bats are only fo...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v6051911
更新日期：2014-04-29 00:00:00
abstract：:Many blood-feeding arthropods are known vectors of viruses that are a source of unprecedented global health concern. Mosquitoes are an integral part of these arthropod vectors. Advancements in next-generation sequencing and bioinformatics has expanded our knowledge on the richness of viruses harbored by arthropods. In...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v10010030
更新日期：2018-01-12 00:00:00
abstract：:During the last 25 years, swine vesicular disease (SVD) has occurred in Italy mostly sub-clinically. Therefore, regular testing of fecal samples from suspected holdings and high turnover premises was fundamental to identifying virus circulation and to achieve SVD eradication. In this study, we evaluated diagnostic per...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12111336
更新日期：2020-11-20 00:00:00
abstract：:Kobuviruses comprise three species, the Aichivirus A, Aichivirus B, and Aichivirus C (porcine kobuvirus). Porcine kobuvirus is endemic to pig farms and is not restricted geographically but, rather, is distributed worldwide. The complete genomic sequences of four porcine kobuvirus strains isolated during a diarrhea out...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v5102548
更新日期：2013-10-18 00:00:00
abstract：:Herpesviruses use a vesicle-mediated transfer of intranuclearly assembled nucleocapsids through the nuclear envelope (NE) for final maturation in the cytoplasm. The molecular basis for this novel vesicular nucleo-cytoplasmic transport is beginning to be elucidated in detail. The heterodimeric viral nuclear egress comp...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v8100266
更新日期：2016-09-27 00:00:00
abstract：:Three dsRNAs, in sizes of approximately 2.5⁻5 kbp, were detected in the plant pathogenic fungus Nigrospora oryzae strain CS-7.5-4. Genomic analysis showed that the 5.0 kb dsRNA was a victorivirus named as Nigrospora oryzae victorivirus 2 (NoRV2). The genome of NoRV2 was 5166 bp in length containing two overlapping ope...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11010083
更新日期：2019-01-19 00:00:00
abstract：:Since the development of methods for inserting and expressing genes in baculoviruses, a line of research has focused on developing recombinant baculoviruses that express insecticidal peptides and proteins. These recombinant viruses have been engineered with the goal of improving their pesticidal potential by shortenin...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v7010422
更新日期：2015-01-21 00:00:00
abstract：:Emergence of human immunodeficiency virus type 1 (HIV-1) drug resistance arises from mutation fixation in the viral genome during antiretroviral therapy. Primary mutations directly confer antiviral drug resistance, while secondary mutations arise that do not confer drug resistance. The A62V amino acid substitution in ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v10070376
更新日期：2018-07-19 00:00:00
abstract：:Porcine circovirus 3 (PCV3) has been identified as a putative swine pathogen with a subset of infections resulting in stillborn and mummified fetuses, encephalitis and myocarditis in perinatal, and periarteritis in growing pigs. Three PCV3 isolates were isolated from weak-born piglets or elevated stillborn and mummifi...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12020219
更新日期：2020-02-16 00:00:00
abstract：:Respiratory viral infections are strongly associated with asthma exacerbations. Rhinovirus is most frequently-detected pathogen; followed by respiratory syncytial virus; metapneumovirus; parainfluenza virus; enterovirus and coronavirus. In addition; viral infection; in combination with genetics; allergen exposure; mic...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v10120682
更新日期：2018-12-01 00:00:00
abstract：:Persistent circulative transmission of plant viruses involves complex interactions between the transmitted virus and its insect vector. Several studies have shown that insect vector proteins are involved in the passage and the transmission of the virus. Interestingly, proteins expressed by bacterial endosymbionts that...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v5061516
更新日期：2013-06-19 00:00:00
abstract：:: African swine fever is a devastating hemorrhagic infectious disease, which affects domestic and wild swines (Susscrofa) of all breeds and ages, with a high lethality of up to 90-100% in naïve animals. The causative agent, African swine fever virus (ASFV), is a large and complex double-stranded DNA arbovirus which is...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v11100882
更新日期：2019-09-20 00:00:00
abstract：:Bovine viral diarrhea virus (BVDV) is an important pathogen belonging to the Pestivirus genus, Flaviviridae family, which comprises viral species that causes an economic impact in animal production. Cattle are the natural host of BVDV and the main source of infection for pigs and other animal species. Due to its antig...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v12060600
更新日期：2020-05-31 00:00:00
abstract：:Despite significant improvements in therapy, the HIV/AIDS pandemic remains an important threat to public health. Current treatments fail to eradicate HIV as proviral DNA persists in long-living cellular reservoirs, leading to viral rebound whenever treatment is discontinued. Hence, a better understanding of viral rese...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v11010012
更新日期：2018-12-26 00:00:00
abstract：:The Vibrio cholerae biotype "El Tor" is responsible for all of the current epidemic and endemic cholera outbreaks worldwide. These outbreaks are clonal, and it is hypothesized that they originate from the coastal areas near the Bay of Bengal, where the lytic bacteriophage ICP1 (International Centre for Diarrhoeal Dise...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v10060299
更新日期：2018-06-01 00:00:00
abstract：:Interferon (IFN) regulatory factor 3 (IRF3) is the key transcription factor for the induction of IFN and antiviral genes. The absence of antiviral genes in IRF3 deficiency leads to susceptibility to a wide range of viral infections. Previously, we uncovered a function for nontranscriptional IRF3 (nt-IRF3), RLR (RIG-I-...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12040442
更新日期：2020-04-14 00:00:00
abstract：:Hepatitis B virus (HBV) persistence is at the basis of HBV reactivation as a consequence of chemotherapy and immunosuppressive treatments. The identification of early viral replication indicators and markers of effective HBV immunological control would be useful in monitoring patients who are at risk of potential vira...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v11090783
更新日期：2019-08-25 00:00:00
abstract：:Purinergic receptors are inflammatory mediators activated by extracellular nucleotides released by dying or injured cells. Several studies have described an important role for these receptors in HIV-1 entry, particularly regarding their activity on HIV-1 viral membrane fusion. Several reports identify purinergic recep...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v12030290
更新日期：2020-03-07 00:00:00
abstract：:With the inclusion of new members, understanding about evolutionary mechanisms and processes by which members of the proposed order, Megavirales, have evolved has become a key area of interest. The central role of gene acquisition has been shown in previous studies. However, the major drawback in gene acquisition stud...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v9040072
更新日期：2017-04-07 00:00:00
abstract：:Hepatitis C is a major global health burden with an estimated 160 million infected individuals worldwide. This long-term disease evolves slowly, often leading to chronicity and potentially to liver failure. There is no anti-HCV vaccine, and, until recently, the only treatment available, based on pegylated interferon a...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v4102197
更新日期：2012-10-17 00:00:00
abstract：:Non-human primates (NHP) are the only animal model suitable to evaluate the protection efficacy of HIV-1 vaccines. It is important to understand how and when neutralizing antibodies (nAbs) with specificities similar to those of human broadly neutralizing antibodies (bnAbs) develop in NHPs. To address these questions, ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12020163
更新日期：2020-01-31 00:00:00
abstract：:Background: Bats are known to host a number of nonpathogenic viruses, as well as highly pathogenic viruses causing fatal diseases like rabies. Serological surveys as part of active and passive bat rabies surveillance mainly use seroneutralization assays, demonstrating the presence of lyssavirus-specific antibodies in ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12030271
更新日期：2020-02-28 00:00:00
abstract：:Ringspot symptoms in European mountain ash (Sorbus aucuparia L.), fig mosaic, rose rosette, raspberry leaf blotch, pigeonpea sterility mosaic (Cajanus cajan) and High Plains disease of maize and wheat were found to be associated with viruses that share several characteristics. They all have single-stranded multipartit...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v4091515
更新日期：2012-09-01 00:00:00
abstract：:Zika virus (ZIKV) is an emerging mosquito-borne pathogen associated with microcephaly and other congenital abnormalities in newborns as well as neurologic complications in adults. The explosive transmission of the virus in the last ten years put it in the limelight and improved our understanding of its biology and pat...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v12090926
更新日期：2020-08-23 00:00:00
abstract：:Human T-cell leukemia virus type 1 (HTLV-1) is an important cancer-causing human retrovirus that has infected approximately 15 million individuals worldwide. Many aspects of HTLV-1 replication, including virus particle structure and assembly, are poorly understood. Group-specific antigen (Gag) proteins labeled at the ...
journal_title：Viruses
pub_type： 杂志文章
doi：10.3390/v9070191
更新日期：2017-07-19 00:00:00
abstract：:Tetraspanins are integral transmembrane proteins organized in microdomains displaying specific and direct interactions with other tetraspanins and molecular partners. Among them, CD81 has been implicated in a variety of physiological and pathological processes. CD81 also plays a crucial role in pathogen entry into hos...
journal_title：Viruses
pub_type： 杂志文章,评审
doi：10.3390/v6020875
更新日期：2014-02-18 00:00:00
