Abstract:
BACKGROUND:The first step of designing any genome-based molecular diagnostic test is to find a specific target sequence. The modified genome comparison method is one of the easiest and most comprehensive ways to achieve this goal. In this study, we aimed to explain this method with the example of Mycobacterium tuberculosis complex and investigate its efficacy in a diagnostic test. METHODS:A specific target was identified using modified genome comparison method and an in-house PCR test was designed. To determine the analytical sensitivity and specificity, 10 standard specimens were used. Also, 230 specimens were used to determine the clinical sensitivity and specificity. RESULTS:The identity and query cover of our new diagnostic target (5KST) were ≥ 90% with M. tuberculosis complex. The 5KST-PCR sensitivity was 100% for smear-positive, culture-positive and 85.7% for smear-negative, culture-positive specimens. All of 100 smear-negative, culture-negative specimens were negative in 5KST-PCR (100% clinical specificity). Analytical sensitivity of 5KST-PCR was approximately 1 copy of genomic DNA per microliter. CONCLUSIONS:Modified genome comparison method is a confident way to find specific targets for use in diagnostic tests. Accordingly, the 5KST-PCR designed in this study has high sensitivity and specificity and can be replaced for conventional TB PCR tests.
journal_name
BMC Infect Disjournal_title
BMC infectious diseasesauthors
Neshani A,Kamali Kakhki R,Sankian M,Zare H,Hooshyar Chichaklu A,Sayyadi M,Ghazvini Kdoi
10.1186/s12879-018-3417-xsubject
Has Abstractpub_date
2018-10-12 00:00:00pages
517issue
1issn
1471-2334pii
10.1186/s12879-018-3417-xjournal_volume
18pub_type
杂志文章abstract:BACKGROUND:Several symptoms are classically thought to be suggestive of pertussis in children, but the diagnostic value of these symptoms in adolescent and adult patients is unclear. We evaluated the accuracy of the clinical findings for the early presumptive diagnosis of pertussis in adolescent and adult patients. Fur...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-129
更新日期:2013-03-11 00:00:00
abstract:BACKGROUND:Chronic osteomyelitis due to direct bone trauma or vascular insufficiency is a frequent problem in orthopaedic surgery. In contrast, acute haematogenous osteomyelitis represents a rare entity that almost exclusively affects prepubescent children or immunodeficient adults. CASE PRESENTATION:In this article, ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-266
更新日期:2013-06-05 00:00:00
abstract:BACKGROUND:Contact tracing of tuberculosis (TB) patients is rarely performed in low-income countries. Our objective was to assess the outcome of and compliance with directly observed treatment (DOTS) of TB patients over a 3 year period in rural Lao PDR. METHODS:We performed a retrospective cohort study in which we enr...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-10-265
更新日期:2010-09-10 00:00:00
abstract:BACKGROUND:Studies report serious adherence problems among youth (individuals age 15-24 years of age) in Uganda. Recent growth in mobile phone ownership has highlighted the potential of using text-based interventions to improve antiretroviral treatment (ART) adherence among Ugandan youth. We piloted a randomized contro...
journal_title:BMC infectious diseases
pub_type: 杂志文章,随机对照试验
doi:10.1186/s12879-020-4896-0
更新日期:2020-02-24 00:00:00
abstract:BACKGROUND:A novel Ehrlichia, closely related to Ehrlichia ruminantium, was recently discovered from Panola Mountain State Park, GA, USA. We conducted a study to determine if this agent was recently introduced into the United States. METHODS:We developed a sensitive PCR assay based on the conserved gltA (citrate synth...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-8-54
更新日期:2008-04-23 00:00:00
abstract:BACKGROUND:The Interleukin 28B (IL28B) rs12979860 polymorphisms was recently reported to be associated with the human T-cell leukemia virus type 1 (HTLV-1) proviral load (PvL) and the development of the HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). METHODS:In an attempt to examine this hypothesi...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-12-374
更新日期:2012-12-23 00:00:00
abstract:BACKGROUND:The purposes of this study were to determine the incidence of central and peripheral venous catheter-related bacteraemias, the relationship between the suspected and final confirmed bacteraemia origins, and the differences in microbiological, epidemiological, clinical, and analytical characteristics between ...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4505-2
更新日期:2019-10-15 00:00:00
abstract:BACKGROUND:Surveillance of current changes in the epidemiology of Invasive Fungal Diseases (IFDs) as an important component of the antifungal stewardship programs (ASP), requires careful regular monitoring, especially in high-risk settings such as oncology centers. This study aimed to examine Candida colonization statu...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4372-x
更新日期:2019-08-30 00:00:00
abstract:BACKGROUND:Changes of miRNAs in exosome have been reported in different disease diagnosis and provided as potential biomarkers. In this study, we compared microRNA profile in exosomes in 5 MHFMD and 5 ESHFMD as well as in 5 healthy children. METHODS:Different expression of miRNAs in exosomes across all the three group...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-506
更新日期:2014-09-17 00:00:00
abstract:BACKGROUND:The potential impact of an influenza pandemic can be assessed by calculating a set of transmissibility parameters, the most important being the reproduction number (R), which is defined as the average number of secondary cases generated per typical infectious case. METHODS:We conducted a systematic review t...
journal_title:BMC infectious diseases
pub_type: 杂志文章,评审
doi:10.1186/1471-2334-14-480
更新日期:2014-09-04 00:00:00
abstract:BACKGROUND:Pandemic influenza vaccine manufacturing capacity and distribution agility is enhanced through the availability of equivalent antigen-sparing vaccines. We evaluated equivalence in terms of immunogenicity between GlaxoSmithKline Vaccines' A/California/7/2009 (H1N1)v-like-AS03 vaccines manufactured in Dresden ...
journal_title:BMC infectious diseases
pub_type: 杂志文章,随机对照试验
doi:10.1186/1471-2334-13-435
更新日期:2013-09-16 00:00:00
abstract:BACKGROUND:Within the genus Aspergillus, A. flavus is the second most important species of clinical significance. It is predominantly associated with infections involving sinuses, eye and skin, mostly in geographic regions with hot and arid climate, including the Middle East. Recent reports on emergence of resistance t...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-126
更新日期:2013-03-06 00:00:00
abstract:BACKGROUND:The WHO guidelines for the management of advanced HIV disease recommend a package of care consisting of rapid initiation of antiretroviral therapy (ART), enhanced screening and diagnosis of tuberculosis (TB) and cryptococcal meningitis, co-trimoxazole prophylaxis, isoniazid preventive therapy (IPT), fluconaz...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-3826-5
更新日期:2019-03-15 00:00:00
abstract::Hepatitis C virus infection (HCV) is one of the most pressing health emergencies in the world with a global prevalence of about 170 million people chronically infected worldwide. In Europe, Italy has the highest HCV prevalence (3 - 4.4%) with peaks of 12.6 - 26% in Southern regions and the major islands. In Italy HCV ...
journal_title:BMC infectious diseases
pub_type: 杂志文章,评审
doi:10.1186/1471-2334-12-S2-S5
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Despite the considerable efforts made to address the issue of brucellosis worldwide, its prevalence in dairy products continues to be difficult to estimate and represents a key public health issue around the world today. The aim of the present study was to better understand the epidemiology of this disease i...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-4946-7
更新日期:2020-03-27 00:00:00
abstract:BACKGROUND:Streptococcus pneumoniae is a commensal of the human upper respiratory tract and a major cause of morbidity and mortality worldwide. This paper presents the distribution of serotypes and antimicrobial resistance in commensal S. pneumoniae strains cultured from healthy carriers older than four years of age in...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-018-3341-0
更新日期:2018-08-29 00:00:00
abstract:BACKGROUND:Bloodstream infections due to Candida species cause significant morbidity and mortality, and the epidemiology of Candida infection is changing. Surveillance for candidemia is necessary to detect trends in species distribution and antifungal resistance. METHODS:The medical and electronic records of all patie...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-13-337
更新日期:2013-07-22 00:00:00
abstract:BACKGROUND:Dengue, a viral disease transmitted by Aedes mosquitoes, is an important public health concern throughout Thailand. Climate variables are potential predictors of dengue transmission. Associations between climate variables and dengue have usually been performed on large-scale first-level national administrati...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4379-3
更新日期:2019-08-23 00:00:00
abstract:BACKGROUND:In Lao PDR, the epidemiology of varicella infection is uncertain, since it is not a notifiable disease and VZV outbreaks are rarely reported as fever/rash (F/R) diseases. METHODS:We estimated the seroprevalence of VZV (IgG ELISA) in different age cohorts (9 months to 46 years; N = 3139) and investigated VZV...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-3990-7
更新日期:2019-05-08 00:00:00
abstract:BACKGROUND:There currently is a severe Zika Virus (ZIKV) epidemic in Brazil and other South American countries. Due to international travel, this poses severe public health risk of ZIKV importation to other countries. We estimate the prevalence of ZIKV in an import region by the time a microcephaly case is detected, si...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-016-2076-z
更新日期:2016-12-12 00:00:00
abstract:BACKGROUND:Lassa fever has been a public health concern in the West African sub-region where it is endemic and a latent threat to the world at large. We investigated the trend in Lassa fever research using bibliometric approach. METHODS:We used the SCOPUS database employing "Lassa fever" as search descriptor. The most...
journal_title:BMC infectious diseases
pub_type: 历史文章,杂志文章
doi:10.1186/s12879-018-3526-6
更新日期:2018-12-10 00:00:00
abstract:BACKGROUND:To verify whether a daily service of Infectious Diseases consultation (ID-cons) is more effective than a weekly service in reducing antibiotic (ATB) consumption without worsening of clinical outcomes. METHODS:Two-year observational analysis of the ID-cons provided in a hospital setting in Milan, Italy. ID-c...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05550-1
更新日期:2020-11-07 00:00:00
abstract:BACKGROUND:Eight genotypes of Hepatitis B virus designated A-H, have been known but in Pakistan, no such data is available on the prevalent HBV genotypes. Therefore, the subject study was conducted to determine HBV genotypes in the indigenous Pakistani population. METHODS:A total of 690 individuals were enrolled for H...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-7-115
更新日期:2007-10-08 00:00:00
abstract:BACKGROUND:This paper studies the effect of mosquito abundance and malaria incidence in the last 3 weeks, and their interaction, on the hazard of time to malaria in a previously studied cohort of children in Ethiopia. METHODS:We model the mosquito abundance and time to malaria data jointly in a Bayesian framework. RE...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-017-2496-4
更新日期:2017-06-12 00:00:00
abstract:BACKGROUND:To reach non-participants, reluctant to undergo clinician-based cervical cancer screening and vaginal self-sampling, urine collection for high-risk human papillomavirus detection (hrHPV) may be valuable. Using two hrHPV DNA assays, we evaluated the concordance of hrHPV positivity in urine samples in comparis...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05663-7
更新日期:2020-12-04 00:00:00
abstract:BACKGROUND:The increasing occurrence of multidrug resistant (MDR) bacteria arises at a time when there is a lack of antibiotics active against these pathogens and few new antimicrobials are in the pipelines of the pharmaceutical industry. Treatment of ventilator-associated pneumonia (VAP) caused especially by MDR Gram-...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-14-135
更新日期:2014-11-28 00:00:00
abstract:BACKGROUND:Almost half the world's population is infected with Helicobacter pylori (H. pylori) with the highest reported prevalence from Africa. This infection is associated with several morbid gastrointestinal conditions. Understanding the trends in seroprevalence and the factors associated with H. pylori seropositivi...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-3677-0
更新日期:2019-01-08 00:00:00
abstract:BACKGROUND:Adjustment of immunosuppression is the main therapy for BK polyomavirus (BKPyV)-associated nephropathy (BKPyVAN) after kidney transplantation (KT). Studies of BKPyV-specific T cell immune response are scarce. Here, we investigated BKPyV-specific T cell immunity in KT recipients diagnosed with BKPyVAN. METHO...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-019-4615-x
更新日期:2019-11-19 00:00:00
abstract:BACKGROUND:Legionnaire's disease is one of the major causes of community-acquired pneumonia and is occasionally complicated by neurological symptoms. However, reports of ocular lesions due to Legionnaire's disease are limited. CASE PRESENTATION:We report the case of a patient with Legionnaire's disease presenting as b...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/s12879-020-05715-y
更新日期:2021-01-07 00:00:00
abstract:BACKGROUND:Adherence of Streptococcus pneumoniae bacteria to lung cells is a first step in the progression from asymptomatic carriage to pneumonia. Adherence abilities vary widely among S. pneumoniae patient isolates. In this study, the binding properties of S. pneumoniae isolates and the effects of binding on activati...
journal_title:BMC infectious diseases
pub_type: 杂志文章
doi:10.1186/1471-2334-6-71
更新日期:2006-04-11 00:00:00