Molecular epidemiology of Hepatitis B virus genotypes in Pakistan.

Abstract:

BACKGROUND:Eight genotypes of Hepatitis B virus designated A-H, have been known but in Pakistan, no such data is available on the prevalent HBV genotypes. Therefore, the subject study was conducted to determine HBV genotypes in the indigenous Pakistani population. METHODS:A total of 690 individuals were enrolled for HBV screening with EIA and nested PCR. Positive samples were further analyzed to determine HBV genotypes (A-F) by multiplex-PCR using type specific primers. RESULTS:110 (15.94%) individuals were positive for HBV, including 64% males and 36% females. Out of these, 66 samples (65.34%) were classified into genotype D, 27 (26.73%) were of genotype B while 5(4.95%) had genotype A. In 3 (2.98%) samples, multiple genotypes were detected (genotype A+B; 2(1.99%) and genotypes B+D; 1(0.99%). Nine (8.18%) samples remained untyable. CONCLUSION:In Asia, genotypes B and C are the most prevalent but our study reveals that genotype D is predominant and HBV infection constitutes a significant health problem in Pakistan.

journal_name

BMC Infect Dis

journal_title

BMC infectious diseases

authors

Alam MM,Zaidi SZ,Malik SA,Shaukat S,Naeem A,Sharif S,Angez M,Butt JA

doi

10.1186/1471-2334-7-115

subject

Has Abstract

pub_date

2007-10-08 00:00:00

pages

115

issn

1471-2334

pii

1471-2334-7-115

journal_volume

7

pub_type

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