Abstract:
BACKGROUND:Metastasis is the main cause of death for lung cancer patients. The ex vivo 4D acellular lung model has been shown to mimic this metastatic process. However, the main concern is the model's lack of cellular components of the tumor's microenvironment. In this study, we aim to determine if the intact lung microenvironment will still allow lung cancer metastasis to form. METHODS:We harvested a heart-lung block from a rat and placed it in a bioreactor after cannulating the pulmonary artery, trachea and tying the right main bronchus for 10-15 days without any tumor cells as a control group or with NSCLC (A549, H1299 or H460), SCLC (H69, H446 or SHP77) or breast cancer cell lines (MCF7 or MDAMB231) through the trachea. We performed lobectomy, H&E staining and IHC for human mitochondria to determine the primary tumor's growth and formation of metastatic lesions. In addition, we isolated circulating tumor cells (CTC) from the model seeded with GFP tagged cells. RESULTS:In the control group, no gross tumor nodules were found, H&E staining showed hyperplastic cells and IHC showed no staining for human mitochondria. All of the models seeded with cancer cell lines formed gross primary tumor nodules that had microscopic characteristics of human cancer cells on H&E staining with IHC showing staining for human mitochondria. CTC were isolated for those cells labeled with GFP and they were viable in culture. Finally, all cell lines formed metastatic lesions with cells stained for human mitochondria. CONCLUSION:The cellular ex vivo 4D model shows that human cancer cells can form a primary tumor, CTC and metastatic lesions in an intact cellular environment. This study suggests that the natural matrix scaffold is the only necessary component to drive metastatic progression and that cellular components play a role in modulating tumor progression.
journal_name
BMC Cancerjournal_title
BMC cancerauthors
Mishra DK,Miller RA,Pence KA,Kim MPdoi
10.1186/s12885-018-4358-xsubject
Has Abstractpub_date
2018-04-18 00:00:00pages
441issue
1issn
1471-2407pii
10.1186/s12885-018-4358-xjournal_volume
18pub_type
杂志文章相关文献
BMC CANCER文献大全abstract:BACKGROUND:Colorectal-cancer is a leading cause of cancer death in the United States, and Latinos have particularly low rates of screening. Strategies and Opportunities to STOP Colon Cancer in Priority Populations (STOP CRC) is a partnership among two research institutions and a network of safety net clinics to promote...
journal_title:BMC cancer
pub_type: 临床试验,杂志文章,多中心研究
doi:10.1186/1471-2407-14-55
更新日期:2014-02-26 00:00:00
abstract:BACKGROUND:To assess the diagnostic value of retrospective PET-MRI fusion and to compare the results with side-by-side analysis and single modality use of PET and of MRI alone for locoregional tumour and nodal staging of head-and-neck cancer. METHODS:Thirty-three patients with head-and-neck cancer underwent preoperati...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-14-846
更新日期:2014-11-19 00:00:00
abstract:BACKGROUND:There is limited real-world safety information on palbociclib for treatment of advanced stage HR+/HER2- breast cancer. METHODS:We conducted a cohort study of breast cancer patients initiating palbociclib and fulvestrant from February 2015 to September 2017 using the HealthCore Integrated Research Database (...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-021-07790-z
更新日期:2021-01-25 00:00:00
abstract:BACKGROUND:To evaluate the role of blood vascular endothelial growth factor (VEGF) kinetics in patients with locally advanced esophageal squamous cell carcinoma (ESCC) receiving curative concurrent chemoradiotherapy (CCRT). METHODS:A total of 97 locally advanced ESCC patients were enrolled. All the patients had their ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4731-9
更新日期:2018-08-20 00:00:00
abstract:BACKGROUND:Migration is important for the metastatic capacity and thus for the malignancy of cancer cells. There is limited knowledge on regulatory factors that promote the migration of neuroblastoma cells. This study investigates the hypothesis that protein kinase C (PKC) isoforms regulate neuroblastoma cell motility....
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-8-365
更新日期:2008-12-11 00:00:00
abstract:BACKGROUND:HER3 mediates drug resistance against epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (TKIs), resulting in tumor relapse in lung cancers. Previously, we demonstrated that EGFR induces HER3 overexpression, which facilitates the formation of cancer stem-like tumorspheres. However, the cellul...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-019-6217-9
更新日期:2019-10-16 00:00:00
abstract:BACKGROUND:Neuroblastoma patients show heterogeneous clinical courses ranging from life-threatening progression to spontaneous regression. Recently, gene expression profiles of neuroblastoma tumours were associated with clinically different phenotypes. However, such data is still rare for important patient subgroups, s...
journal_title:BMC cancer
pub_type: 杂志文章,meta分析,评审
doi:10.1186/1471-2407-7-89
更新日期:2007-05-25 00:00:00
abstract:BACKGROUND:Colorectal cancer screening is key to early detection and thus to early treatment, but uptake is often sub-optimal, particularly amongst lower income groups. It is proposed that the imminent introduction of the single-sample Faecal Immunochemical Test (FIT) in Scotland may lead to increased uptake as compare...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-016-2133-4
更新日期:2016-02-13 00:00:00
abstract:BACKGROUND:Human papillomaviruses (HPV) are the aetiological agents of certain benign and malignant tumours of skin and mucosae; the most important of which is cervical cancer. Also, the incidence of ano-genital warts, HPV-anal cancer and oropharyngeal cancers are rising. To help ascertain a useful PCR detection protoc...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-9-440
更新日期:2009-12-15 00:00:00
abstract:BACKGROUND:Estrogen (17β-estradiol) promotes the survival and proliferation of breast cancer cells and its receptors represent important therapeutic targets. The cellular actions of estrogen are mediated by the nuclear estrogen receptors ERα and ERβ as well as the 7-transmembrane spanning G protein-coupled estrogen rec...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1699-6
更新日期:2015-10-15 00:00:00
abstract:BACKGROUND:Sexually transmitted Human Papilloma Virus (HPV) infection is a known risk factor for cancer of the anal canal in both men and women. CASE PRESENTATION:We describe a report of synchronous carcinoma of the anal canal in a heterosexual couple. High risk type 16 HPV DNA was detected in both tumors. CONCLUSION...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4785-8
更新日期:2018-09-10 00:00:00
abstract:BACKGROUND:High-grade serous ovarian cancer is a detrimental disease. Treatment options in patients with a recurrent disease are dependent on BRCA1/2 mutation status since only patients with known BRCA mutation are eligible for treatment with poly(ADP-ribose) polymerase inhibitors (PARPi). The aim of this study was to ...
journal_title:BMC cancer
pub_type: 临床试验,杂志文章
doi:10.1186/s12885-019-5535-2
更新日期:2019-04-02 00:00:00
abstract:BACKGROUND:This paper describes the development of a Cancer Awareness Measure for colorectal (CRC) cancer (Bowel/Colorectal CAMa) (study 1) and presents key results from a population-representative survey using the measure (study 2). METHODS:STUDY 1: Items were taken from the literature and reviewed by expert groups. ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-11-366
更新日期:2011-08-23 00:00:00
abstract:BACKGROUND:Young breast cancer (BC) patients less than 45 years old are at higher risk of dying from the disease when compared to their older counterparts. However, specific risk factors leading to this poorer outcome have not been identified. METHODS:One candidate is iron deficiency, as this is common in young women ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-13-307
更新日期:2013-06-24 00:00:00
abstract:BACKGROUND:Clinical studies implying the sunitinib multi-kinase inhibitor have led to disappointing results for breast cancer care but mostly focused on HER2-negative subtypes. Preclinical researches involving this drug mostly concern Triple Negative Breast Cancer (TNBC) murine models. Here, we explored the therapeutic...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1540-2
更新日期:2015-07-22 00:00:00
abstract:BACKGROUND:Acrokeratosis paraneoplastica is a rare paraneoplastic phenomenon associated with upper aerodigestive tract carcinomas, usually manifesting as psoriasiform keratosis over the acral sites. It is primarily seen in white males above the age of 40 years. Here we report a case of paraneoplastic acrokeratosis in a...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1527-z
更新日期:2015-07-08 00:00:00
abstract:BACKGROUND:The Ras-dependent ERK1/2 MAP kinase signaling pathway plays a central role in cell proliferation control and is frequently activated in human colorectal cancer. Small-molecule inhibitors of MEK1/MEK2 are therefore viewed as attractive drug candidates for the targeted therapy of this malignancy. However, the ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-8-337
更新日期:2008-11-17 00:00:00
abstract:BACKGROUND:The tumor microenvironment plays a determinative role in stimulating tumor progression and metastasis. Notably, tumor-stroma signals affect the pattern of infiltrated immune cells and the profile of tumor-released cytokines. Among the known molecules that are engaged in stimulating the metastatic spread of t...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1034-2
更新日期:2015-02-12 00:00:00
abstract:BACKGROUND:Conditional survival (CS) measures the probability that patients will survive an additional number of years given that they have already survived for a certain period of time. METHODS:In total, 2935 gastric cancer patients who had undergone curative gastrectomy between 1995 and 2011 were enrolled. The Cox p...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-2022-2
更新日期:2015-12-23 00:00:00
abstract:BACKGROUND:This study aimed to evaluate the perioperative outcomes and prognostic impact of the consecutive steps of imaging, frailty assessment, and diagnostic laparoscopy (DLS) in patients with advanced epithelial ovarian cancer (EOC). METHODS:Patients diagnosed with EOC during 2012-2015 were analyzed retrospectivel...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3476-1
更新日期:2017-07-12 00:00:00
abstract:BACKGROUND:In China, there are currently no approved therapies for the treatment of metastatic renal cell carcinoma (mRCC) following progression with vascular endothelial growth factor (VEGF)-targeted agents. In the phase 3 RECORD-1 trial, the mammalian target of rapamycin (mTOR) inhibitor everolimus afforded clinical ...
journal_title:BMC cancer
pub_type: 杂志文章,多中心研究
doi:10.1186/1471-2407-13-136
更新日期:2013-03-21 00:00:00
abstract:BACKGROUND:There is evidence that some ovarian tumours evoke an immune response, which can be assessed by tumour infiltrating lymphocytes (TILs). To facilitate adoption of TILs as a clinical biomarker, a standardised method for their H&E visual evaluation has been validated in breast cancer. METHODS:We sought to inves...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-017-3585-x
更新日期:2017-09-20 00:00:00
abstract::Following publication of the original article [1], the authors reported an error in Fig. 6c and in the figure legends for Fig. 5c and Fig. 6c. ...
journal_title:BMC cancer
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12885-020-6552-x
更新日期:2020-01-23 00:00:00
abstract:BACKGROUND:The only available predictive models for the outcome of breast cancer patients in New Zealand (NZ) are based on data in other countries. We aimed to develop and validate a predictive model using NZ data for this population, and compare its performance to a widely used overseas model, the Nottingham Prognosti...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-018-4791-x
更新日期:2018-09-17 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:BMC cancer
pub_type: 已发布勘误
doi:10.1186/s12885-020-07704-5
更新日期:2020-12-07 00:00:00
abstract:BACKGROUND:The APC tumor suppressor is mutated or downregulated in many tumor types, and is prominently localized to punctate clusters at protrusion tips in migratory cells, such as in astrocytes where it has been implicated in directed cell motility. Although APC loss is considered an initiating event in colorectal ca...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-13-12
更新日期:2013-01-09 00:00:00
abstract:BACKGROUND:RNA from exosomes and other microvesicles contain transcripts of tumour origin. In this study we sought to identify biomarkers of glioblastoma multiforme in microvesicle RNA from serum of affected patients. METHODS:Microvesicle RNA from serum from patients with de-novo primary glioblastoma multiforme (N = 9...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-12-22
更新日期:2012-01-17 00:00:00
abstract:BACKGROUND:Carbonic anhydrase IX (CA IX) is a tumor-associated, highly active, transmembrane carbonic anhydrase isoform regulated by hypoxia and implicated in pH control and adhesion-migration-invasion. CA IX ectodomain (ECD) is shed from the tumor cell surface to serum/plasma of patients, where it can signify cancer p...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-016-2267-4
更新日期:2016-03-19 00:00:00
abstract:BACKGROUND:Adoptive immunotherapy with cytotoxic T lymphocytes (CTL) represents an alternative approach to treating solid tumors. Ideally, this would confer long-term protection against tumor. We previously demonstrated that in vitro-generated tumor-specific CTL from the ovalbumin (OVA)-specific OT-I T cell receptor tr...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/1471-2407-3-21
更新日期:2003-07-28 00:00:00
abstract:BACKGROUND:Hyponatraemia is a common finding in patients with cancer, and has been shown to be associated with poor prognosis in different settings. We have analysed the impact of severe hyponatraemia in patients with cancer. METHODS:A retrospective review of all patients admitted to a specialist cancer hospital with ...
journal_title:BMC cancer
pub_type: 杂志文章
doi:10.1186/s12885-015-1156-6
更新日期:2015-03-22 00:00:00