Abstract:
BACKGROUND:Morphine has been widely used as a clinical anesthetic and analgesic. However, abuse of morphine might result in psychological and physiological dependence. Previous studies have indicated that memory mechanisms play critical roles in morphine dependence. METHODS:Morphine dependence was established in mice utilizing place preference conditioning (CPP). We observed changes in the methylome and transcriptome of the nucleus accumbens during the reactivation of the memory trace. We also monitored for changes in the methylome and transcriptome of mice that were acutely exposed to morphine. RESULTS:We detected 165 and 18 differentially expressed genes (DEGs) and 6 and 24 significant methyl-sensitive cut counting (MSCC) windows in the acute morphine treatment and the CPP model, respectively. The changes in the methylome and transcriptome during the acute treatment were mainly caused by a response to the morphine stimulus; most of the DEGs were correlated with hormone or transcription factor activity regulation. The expression levels of Lcn2 and Hspb1, which participate in the activation of NF-κB, were significantly decreased in the CPP morphine treatment model. Besides, the alternative splicing of the curtailed isoform of Caps1 was significantly increased in the CPP morphine-treated group, and the methylation levels of Arf4, Vapa, and Gga3 were decreased. These genes play critical roles in the regulation of the Golgi network. CONCLUSIONS:The current study indicates that NF-κB signaling and vesicular transport are correlated with the reactivation of the memory trace in morphine-dependent mice. The results obtained in our study agree with previous observations and identify additional candidate genes for further research. VIRTUAL SLIDES:The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1196707364133126.
journal_name
Diagn Patholjournal_title
Diagnostic pathologyauthors
Ye J,Yang Z,Li C,Cai M,Zhou D,Zhang Q,Wei Y,Wang T,Liu Ydoi
10.1186/1746-1596-9-142subject
Has Abstractpub_date
2014-07-10 00:00:00pages
142issn
1746-1596pii
1746-1596-9-142journal_volume
9pub_type
杂志文章abstract:BACKGROUND:Reduced DNA repair capacities due to inherited polymorphisms may increase the susceptibility to cancers including gastric cancer. Previous studies investigating the association between Xeroderma Pigmentosum group C (XPC) gene polymorphisms and gastric cancer risk reported inconsistent results. We performed a...
journal_title:Diagnostic pathology
pub_type: 杂志文章,meta分析,评审
doi:10.1186/1746-1596-9-96
更新日期:2014-05-23 00:00:00
abstract::Diagnostic surgical pathology or tissue–based diagnosis still remains the most reliable and specific diagnostic medical procedure. The development of whole slide scanners permits the creation of virtual slides and to work on so-called virtual microscopes. In addition to interactive work on virtual slides approaches ha...
journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/1746-1596-6-s1-s12
更新日期:2011-01-01 00:00:00
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journal_title:Diagnostic pathology
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journal_title:Diagnostic pathology
pub_type: 杂志文章,评审
doi:10.1186/s13000-017-0620-9
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journal_title:Diagnostic pathology
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/s13000-018-0707-y
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abstract:BACKGROUND:Histologic grade is the most important predictor of the clinical outcome of non-muscle invasive (Ta, T1) papillary urothelial carcinoma (NMIPUCa), but its ambiguous criteria diminish its power to predict recurrence/progression for individual patients. We attempted to find an objective and reproducible histol...
journal_title:Diagnostic pathology
pub_type: 杂志文章,多中心研究
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/s13000-016-0553-8
更新日期:2016-10-22 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章
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journal_title:Diagnostic pathology
pub_type: 杂志文章
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/s13000-015-0448-0
更新日期:2015-12-16 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章,评审
doi:10.1186/1746-1596-8-131
更新日期:2013-08-05 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/s13000-015-0378-x
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pub_type: 杂志文章
doi:10.1186/s13000-019-0898-x
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/1746-1596-3-30
更新日期:2008-07-12 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/1746-1596-7-113
更新日期:2012-08-29 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/1746-1596-1-11
更新日期:2006-07-17 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章,评审
doi:10.1186/s13000-015-0330-0
更新日期:2015-07-16 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/1746-1596-5-62
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/1746-1596-5-38
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pub_type: 杂志文章,评审
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/1746-1596-3-S1-S6
更新日期:2008-07-15 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/s13000-015-0249-5
更新日期:2015-04-02 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/1746-1596-3-31
更新日期:2008-07-25 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章,评审
doi:10.1186/s13000-017-0613-8
更新日期:2017-03-04 00:00:00
abstract:BACKGROUND:Rapid ("warm") autopsies of patients with advanced metastatic cancer provide important insight into the natural history, pathobiology and histomorphology of disease in treatment-resistant tumors. Plasmacytoid urothelial carcinoma (PUC) is a rare variant of urothelial carcinoma characterized by neoplastic cel...
journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/s13000-019-0896-z
更新日期:2019-10-21 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/1746-1596-9-128
更新日期:2014-06-27 00:00:00
abstract::A case of prenatally diagnosed, giant cord hemangioma is reported, which was accompanied by the elevation of maternal serum alpha-fetoprotein (MS-AFP) and human chorionic gonadotropin (MS-hCG) levels. A 30-year-old woman without a previous history of gravida or para, presented with intermittent abdominal pain at 26 we...
journal_title:Diagnostic pathology
pub_type: 杂志文章,评审
doi:10.1186/s13000-015-0385-y
更新日期:2015-09-04 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/s13000-019-0876-3
更新日期:2019-09-04 00:00:00
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journal_title:Diagnostic pathology
pub_type: 杂志文章
doi:10.1186/1746-1596-7-119
更新日期:2012-09-10 00:00:00