A hypothesis for bacteriophage DNA packaging motors.


:The hypothesis is presented that bacteriophage DNA packaging motors have a cycle comprised of bind/release thermal ratcheting with release-associated DNA pushing via ATP-dependent protein folding. The proposed protein folding occurs in crystallographically observed peptide segments that project into an axial channel of a protein 12-mer (connector) that serves, together with a coaxial ATPase multimer, as the entry portal. The proposed cycle begins when reverse thermal motion causes the connector's peptide segments to signal the ATPase multimer to bind both ATP and the DNA molecule, thereby producing a dwell phase recently demonstrated by single-molecule procedures. The connector-associated peptide segments activate by transfer of energy from ATP during the dwell. The proposed function of connector/ATPase symmetry mismatches is to reduce thermal noise-induced signaling errors. After a dwell, ATP is cleaved and the DNA molecule released. The activated peptide segments push the released DNA molecule, thereby producing a burst phase recently shown to consist of four mini-bursts. The constraint of four mini-bursts is met by proposing that each mini-burst occurs via pushing by three of the 12 subunits of the connector. If all four mini-bursts occur, the cycle repeats. If the mini-bursts are not completed, a second cycle is superimposed on the first cycle. The existence of the second cycle is based on data recently obtained with bacteriophage T3. When both cycles stall, energy is diverted to expose the DNA molecule to maturation cleavage.






Serwer P




Has Abstract


2010-09-01 00:00:00












  • A Novel Sub-Lineage of Chikungunya Virus East/Central/South African Genotype Indian Ocean Lineage Caused Sequential Outbreaks in Bangladesh and Thailand.

    abstract::In recent decades, chikungunya virus (CHIKV) has become geographically widespread. In 2004, the CHIKV East/Central/South African (ECSA) genotype moved from Africa to Indian ocean islands and India followed by a large epidemic in Southeast Asia. In 2013, the CHIKV Asian genotype drove an outbreak in the Americas. Since...


    pub_type: 杂志文章


    authors: Phadungsombat J,Imad H,Rahman M,Nakayama EE,Kludkleeb S,Ponam T,Rahim R,Hasan A,Poltep K,Yamanaka A,Matsee W,Piyaphanee W,Phumratanaprapin W,Shioda T

    更新日期:2020-11-17 00:00:00

  • Following in the Footsteps of the Chikungunya Virus in Brazil: The First Autochthonous Cases in Amapá in 2014 and Its Emergence in Rio de Janeiro during 2016.

    abstract::Currently, Brazil lives a triple arboviruses epidemic (DENV, ZIKV and CHIKV) making the differential diagnosis difficult for health professionals. Here, we aimed to investigate chikungunya cases and the possible occurrence of co-infections during the epidemic in Amapá (AP) that started in 2014 when the first autochtho...


    pub_type: 杂志文章


    authors: de Souza TMA,Ribeiro ED,Corrêa VCE,Damasco PV,Santos CC,de Bruycker-Nogueira F,Chouin-Carneiro T,Faria NRDC,Nunes PCG,Heringer M,Lima MDRQ,Badolato-Corrêa J,Cipitelli MDC,Azeredo EL,Nogueira RMR,Dos Santos FB

    更新日期:2018-11-12 00:00:00

  • Estimating hantavirus risk in southern Argentina: a GIS-based approach combining human cases and host distribution.

    abstract::We use a Species Distribution Modeling (SDM) approach along with Geographic Information Systems (GIS) techniques to examine the potential distribution of hantavirus pulmonary syndrome (HPS) caused by Andes virus (ANDV) in southern Argentina and, more precisely, define and estimate the area with the highest infection p...


    pub_type: 杂志文章


    authors: Andreo V,Neteler M,Rocchini D,Provensal C,Levis S,Porcasi X,Rizzoli A,Lanfri M,Scavuzzo M,Pini N,Enria D,Polop J

    更新日期:2014-01-14 00:00:00

  • Characterization of a Novel Thermobifida fusca Bacteriophage P318.

    abstract::Thermobifida fusca is of biotechnological interest due to its ability to produce an array of plant cell wall hydrolytic enzymes. Nonetheless, only one T. fusca bacteriophage with genome information has been reported to date. This study was aimed at discovering more relevant bacteriophages to expand the existing knowle...


    pub_type: 杂志文章


    authors: Cheepudom J,Lin TL,Lee CC,Meng M

    更新日期:2019-11-08 00:00:00

  • TMPRSS2 and MSPL Facilitate Trypsin-Independent Porcine Epidemic Diarrhea Virus Replication in Vero Cells.

    abstract::Type II transmembrane serine proteases (TTSPs) facilitate the spread and replication of viruses such as influenza and human coronaviruses, although it remains unclear whether TTSPs play a role in the progression of animal coronavirus infections, such as that by porcine epidemic diarrhea virus (PEDV). In this study, TT...


    pub_type: 杂志文章


    authors: Shi W,Fan W,Bai J,Tang Y,Wang L,Jiang Y,Tang L,Liu M,Cui W,Xu Y,Li Y

    更新日期:2017-05-18 00:00:00

  • Antiviral treatment of chronic hepatitis B virus (HBV) infections.

    abstract::While 25 compounds have been formally licensed for the treatment of HIV infection (AIDS), only seven licensed products are currently available for the treatment of chronic hepatitis B virus (HBV) infection: interferon-α, pegylated interferon-α, lamivudine, adefovir (dipivoxil), entecavir, telbivudine and tenofovir (di...


    pub_type: 杂志文章


    authors: De Clercq E,Férir G,Kaptein S,Neyts J

    更新日期:2010-06-01 00:00:00

  • A Taxonomic Review of Clostridium difficile Phages and Proposal of a Novel Genus, "Phimmp04likevirus".

    abstract::Currently, only three phages that infect the medically important bacterium Clostridium difficile have been discussed by the International Committee of Viral Taxonomy (ICTV). They are all myoviruses, and have been assigned to the genus "phicd119likevirus". An additional nine phages have since been described in the lite...


    pub_type: 杂志文章


    authors: Hargreaves KR,Clokie MR

    更新日期:2015-05-21 00:00:00

  • Interferon Regulatory Factor 3-Mediated Signaling Limits Middle-East Respiratory Syndrome (MERS) Coronavirus Propagation in Cells from an Insectivorous Bat.

    abstract::Insectivorous bats are speculated to be ancestral hosts of Middle-East respiratory syndrome (MERS) coronavirus (CoV). MERS-CoV causes disease in humans with thirty-five percent fatality, and has evolved proteins that counteract human antiviral responses. Since bats experimentally infected with MERS-CoV do not develop ...


    pub_type: 杂志文章


    authors: Banerjee A,Falzarano D,Rapin N,Lew J,Misra V

    更新日期:2019-02-13 00:00:00

  • Profiling Transcripts of Vector Competence between Two Different Aedes aegypti Populations in Florida.

    abstract::A Chikungunya virus (CHIKV) outbreak in Italy in 2007 spread to include the islands of the Caribbean and most of the Americas and still circulates in Europe and Africa. Florida being close in distance to the Caribbean islands experienced a CHIKV outbreak in 2014 and continues to have a few travel-related cases each ye...


    pub_type: 杂志文章


    authors: Shin D,Kang S,Smartt CT

    更新日期:2020-07-29 00:00:00

  • Lessons Learned in Developing a Commercial FIV Vaccine: The Immunity Required for an Effective HIV-1 Vaccine.

    abstract::The feline immunodeficiency virus (FIV) vaccine called Fel-O-Vax® FIV is the first commercial FIV vaccine released worldwide for the use in domestic cats against global FIV subtypes (A⁻E). This vaccine consists of inactivated dual-subtype (A plus D) FIV-infected cells, whereas its prototype vaccine consists of inactiv...


    pub_type: 杂志文章,评审


    authors: Sahay B,Yamamoto JK

    更新日期:2018-05-22 00:00:00

  • Antiviral Hammerhead Ribozymes Are Effective for Developing Transgenic Suppression of Chikungunya Virus in Aedes aegypti Mosquitoes.

    abstract::The chikungunya virus (CHIKV) is an emerging pathogen with widespread distribution in regions of Africa, India, and Asia that threatens to spread into temperate climates with the introduction of its major vector, Aedes albopictus. CHIKV causes a disease frequently misdiagnosed as dengue fever, with potentially life-th...


    pub_type: 杂志文章


    authors: Mishra P,Furey C,Balaraman V,Fraser MJ

    更新日期:2016-06-10 00:00:00

  • Portable Rabies Virus Sequencing in Canine Rabies Endemic Countries Using the Oxford Nanopore MinION.

    abstract::As countries with endemic canine rabies progress towards elimination by 2030, it will become necessary to employ techniques to help plan, monitor, and confirm canine rabies elimination. Sequencing can provide critical information to inform control and vaccination strategies by identifying genetically distinct virus va...


    pub_type: 杂志文章


    authors: Gigante CM,Yale G,Condori RE,Costa NC,Long NV,Minh PQ,Chuong VD,Tho ND,Thanh NT,Thin NX,Hanh NTH,Wambura G,Ade F,Mito O,Chuchu V,Muturi M,Mwatondo A,Hampson K,Thumbi SM,Thomae BG,de Paz VH,Meneses S,Munyua P,

    更新日期:2020-11-04 00:00:00

  • The impact of regulations, safety considerations and physical limitations on research progress at maximum biocontainment.

    abstract::We describe herein, limitations on research at biosafety level 4 (BSL-4) containment laboratories, with regard to biosecurity regulations, safety considerations, research space limitations, and physical constraints in executing experimental procedures. These limitations can severely impact the number of collaborations...


    pub_type: 杂志文章,评审


    authors: Shurtleff AC,Garza N,Lackemeyer M,Carrion R Jr,Griffiths A,Patterson J,Edwin SS,Bavari S

    更新日期:2012-12-01 00:00:00

  • Temperature Sensitive Mutations in Influenza A Viral Ribonucleoprotein Complex Responsible for the Attenuation of the Live Attenuated Influenza Vaccine.

    abstract::Live attenuated influenza vaccines (LAIV) have prevented morbidity and mortality associated with influenza viral infections for many years and represent the best therapeutic option to protect against influenza viral infections in humans. However, the development of LAIV has traditionally relied on empirical methods, s...


    pub_type: 杂志文章,评审


    authors: Martínez-Sobrido L,Peersen O,Nogales A

    更新日期:2018-10-15 00:00:00

  • Caging the beast: TRIM5α binding to the HIV-1 core.

    abstract::The potent HIV-1 inhibitor TRIM5α blocks HIV-1 infection by accelerating the uncoating of HIV-1. TRIM5α is known to form higher-order self-association complexes that contribute to the avidity of TRIM5α for the HIV-1 capsid, and are essential to inhibit infection; these higher-order self-association complexes are depen...


    pub_type: 杂志文章


    authors: Diaz-Griffero F

    更新日期:2011-05-01 00:00:00

  • Facing antibiotic resistance: Staphylococcus aureus phages as a medical tool.

    abstract::Staphylococcus aureus is a common and often virulent pathogen in humans. This bacterium is widespread, being present on the skin and in the nose of healthy people. Staphylococcus aureus can cause infections with severe outcomes ranging from pustules to sepsis and death. The introduction of antibiotics led to a general...


    pub_type: 杂志文章,评审


    authors: Kaźmierczak Z,Górski A,Dąbrowska K

    更新日期:2014-07-01 00:00:00

  • Isolation of a Chinook Salmon Bafinivirus (CSBV) in Imported Goldfish Carassius auratus L. in the United Kingdom and Evaluation of Its Virulence in Resident Fish Species.

    abstract::This is the first record of a fish nidovirus isolated from a consignment of goldfish at the United Kingdom (UK) border. The full-length viral genome was 25,985 nt, sharing a 97.9% nucleotide identity with the Chinook salmon bafinivirus (CSBV) NIDO with two deletions of 537 and 480 nt on the ORF Ia protein. To assess t...


    pub_type: 杂志文章


    authors: Cano I,Stone D,Savage J,Wood G,Mulhearn B,Gray J,Stinton N,Ross S,Bonar M,Taylor NGH,Bateman KS,Feist SW

    更新日期:2020-05-25 00:00:00

  • Filoviral immune evasion mechanisms.

    abstract::The Filoviridae family of viruses, which includes the genera Ebolavirus (EBOV) and Marburgvirus (MARV), causes severe and often times lethal hemorrhagic fever in humans. Filoviral infections are associated with ineffective innate antiviral responses as a result of virally encoded immune antagonists, which render the h...


    pub_type: 杂志文章,评审


    authors: Ramanan P,Shabman RS,Brown CS,Amarasinghe GK,Basler CF,Leung DW

    更新日期:2011-09-01 00:00:00

  • Oligopeptide m13 phage display in pathogen research.

    abstract::Phage display has become an established, widely used method for selection of peptides, antibodies or alternative scaffolds. The use of phage display for the selection of antigens from genomic or cDNA libraries of pathogens which is an alternative to the classical way of identifying immunogenic proteins is not well-kno...


    pub_type: 杂志文章,评审


    authors: Kügler J,Zantow J,Meyer T,Hust M

    更新日期:2013-10-16 00:00:00

  • Viral RNA Silencing Suppression: The Enigma of Bunyavirus NSs Proteins.

    abstract::The Bunyaviridae is a family of arboviruses including both plant- and vertebrate-infecting representatives. The Tospovirus genus accommodates plant-infecting bunyaviruses, which not only replicate in their plant host, but also in their insect thrips vector during persistent propagative transmission. For this reason, t...


    pub_type: 杂志文章,评审


    authors: Hedil M,Kormelink R

    更新日期:2016-07-23 00:00:00

  • Fundamental Difficulties Prevent the Reconstruction of the Deep Phylogeny of Viruses.

    abstract::The extension of virology beyond its traditional medical, veterinary, or agricultural applications, now called environmental virology, has shown that viruses are both the most numerous and diverse biological entities on Earth. In particular, virus isolations from unicellular eukaryotic hosts (heterotrophic and photosy...


    pub_type: 杂志文章


    authors: Claverie JM

    更新日期:2020-10-06 00:00:00

  • Phylogenetic Analysis and Characterization of a Sporadic Isolate of Equine Influenza A H3N8 from an Unvaccinated Horse in 2015.

    abstract::Equine influenza, caused by the H3N8 subtype, is a highly contagious respiratory disease affecting equid populations worldwide and has led to serious epidemics and transboundary pandemics. This study describes the phylogenetic characterization and replication kinetics of recently-isolated H3N8 virus from a nasal swab ...


    pub_type: 杂志文章


    authors: Sreenivasan CC,Jandhyala SS,Luo S,Hause BM,Thomas M,Knudsen DEB,Leslie-Steen P,Clement T,Reedy SE,Chambers TM,Christopher-Hennings J,Nelson E,Wang D,Kaushik RS,Li F

    更新日期:2018-01-11 00:00:00

  • Nucleocapsid Structure of Negative Strand RNA Virus.

    abstract::Negative strand RNA viruses (NSVs) include many important human pathogens, such as influenza virus, Ebola virus, and rabies virus. One of the unique characteristics that NSVs share is the assembly of the nucleocapsid and its role in viral RNA synthesis. In NSVs, the single strand RNA genome is encapsidated in the line...


    pub_type: 杂志文章,评审


    authors: Luo M,Terrell JR,Mcmanus SA

    更新日期:2020-07-30 00:00:00

  • βTrCP is Required for HIV-1 Vpu Modulation of CD4, GaLV Env, and BST-2/Tetherin.

    abstract::The Human immunodeficiency virus-1 (HIV-1) accessory protein Vpu modulates numerous proteins, including the host proteins CD4 and BST-2/tetherin. Vpu interacts with the Skp, Cullin, F-Box (SCF) ubiquitin ligase through interactions with the F-Box protein βTrCP (1 and/or 2). This interaction is dependent on phosphoryla...


    pub_type: 杂志文章


    authors: Song YE,Cyburt D,Lucas TM,Gregory DA,Lyddon TD,Johnson MC

    更新日期:2018-10-19 00:00:00

  • Methanolic Extract of Rhizoma Coptidis Inhibits the Early Viral Entry Steps of Hepatitis C Virus Infection.

    abstract::Hepatitis C Virus (HCV) remains an important public health threat with approximately 170 million carriers worldwide who are at risk of developing hepatitis C-associated end-stage liver diseases. Despite improvement of HCV treatment using the novel direct-acting antivirals (DAAs) targeting viral replication, there is a...


    pub_type: 杂志文章


    authors: Hung TC,Jassey A,Lin CJ,Liu CH,Lin CC,Yen MH,Lin LT

    更新日期:2018-11-27 00:00:00

  • Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D.

    abstract::The Pneumoviridae family includes human metapneumovirus (HMPV) and human orthopneumovirus, which is also known as a respiratory syncytial virus (HRSV). These are large enveloped, negative single-strand RNA viruses. HMPV and HRSV are the human members, which commonly infect children. HMPV, which was discovered in 2001,...


    pub_type: 杂志文章


    authors: Diab M,Schmiedel D,Seidel E,Bacharach E,Mandelboim O

    更新日期:2020-07-20 00:00:00

  • Role of Dendritic Cells in Exposing Latent HIV-1 for the Kill.

    abstract::The development of effective yet nontoxic strategies to target the latent human immunodeficiency virus-1 (HIV-1) reservoir in antiretroviral therapy (ART)-suppressed individuals poses a critical barrier to a functional cure. The 'kick and kill' approach to HIV eradication entails proviral reactivation during ART, coup...


    pub_type: 杂志文章,评审


    authors: Kristoff J,Rinaldo CR,Mailliard RB

    更新日期:2019-12-28 00:00:00

  • Serological Survey of Lyssaviruses in Polish Bats in the Frame of Passive Rabies Surveillance Using an Enzyme-Linked Immunosorbent Assay.

    abstract::Background: Bats are known to host a number of nonpathogenic viruses, as well as highly pathogenic viruses causing fatal diseases like rabies. Serological surveys as part of active and passive bat rabies surveillance mainly use seroneutralization assays, demonstrating the presence of lyssavirus-specific antibodies in ...


    pub_type: 杂志文章


    authors: Orłowska A,Smreczak M,Freuling CM,Müller T,Trębas P,Rola J

    更新日期:2020-02-28 00:00:00

  • Genetic Susceptibility to Human Norovirus Infection: An Update.

    abstract::Noroviruses are the most common etiological agent of acute gastroenteritis worldwide. Despite their high infectivity, a subpopulation of individuals is resistant to infection and disease. This susceptibility is norovirus genotype-dependent and is largely mediated by the presence or absence of human histo-blood group a...


    pub_type: 杂志文章,评审


    authors: Nordgren J,Svensson L

    更新日期:2019-03-06 00:00:00

  • Histone deacetylases in herpesvirus replication and virus-stimulated host defense.

    abstract::Emerging evidence highlights a critical role for protein acetylation during herpesvirus infection. As prominent modulators of protein acetylation, histone deacetylases (HDACs) are essential transcriptional and epigenetic regulators. Not surprisingly, viruses have evolved a wide array of mechanisms to subvert HDAC func...


    pub_type: 杂志文章,评审


    authors: Guise AJ,Budayeva HG,Diner BA,Cristea IM

    更新日期:2013-06-27 00:00:00