当前位置： SCI文献检索 > BLOOD期刊下所有文献 > Hydrodynamic shear shows distinct roles for LFA-1 and Mac-1 in neutrophil adhesion to intercellular adhesion molecule-1.

Hydrodynamic shear shows distinct roles for LFA-1 and Mac-1 in neutrophil adhesion to intercellular adhesion molecule-1.

Abstract：

:The binding of neutrophil beta2 integrin to intercellular adhesion molecule-1 (ICAM-1) expressed on the inflamed endothelium is critical for neutrophil arrest at sites of tissue inflammation. To quantify the strength and kinetics of this interaction, we measured the adhesion between chemotactically stimulated neutrophils and ICAM-1-transfected mouse cells (E3-ICAM) in suspension in a cone-plate viscometer at shear rates typical of venular blood flow (100 s-1 to 500 s-1). The kinetics of aggregation were fit with a mathematical model based on two-body collision theory. This enabled estimation of adhesion efficiency, defined as the probability with which collisions between cells resulted in firm adhesion. The efficiency of beta2-integrin-dependent adhesion was highest ( approximately 0.2) at 100 s-1 and it decreased to approximately zero at 400 s-1. Both LFA-1 and Mac-1 contributed equally to adhesion efficiency over the initial 30 seconds of stimulation, but adhesion was entirely Mac-1-dependent by 120 seconds. Two hydrodynamic parameters were observed to influence integrin-dependent adhesion efficiency: the level of shear stress and the intercellular contact duration. Below a critical shear stress (<2 dyn/cm2), contact duration predominantly limited adhesion efficiency. The estimated minimum contact duration for beta2-integrin binding was approximately 6.5 ms. Above the critical shear stress (>2 dyn/cm2), the efficiency of neutrophil adhesion to E3-ICAM was limited by both the contact duration and the tensile stress. We conclude that at low shear, neutrophil adhesion is modulated independently through either LFA-1 or Mac-1, which initially contribute with equal efficiency, but differ over the duration of chemotactic stimulation.

journal_name

Blood

journal_title

Blood

authors

Neelamegham S,Taylor AD,Burns AR,Smith CW,Simon SI

subject

Has Abstract

pub_date

1998-09-01 00:00:00

pages

1626-38

issue

5

eissn

0006-4971

issn

1528-0020

journal_volume

92

pub_type

杂志文章

相关文献

BLOOD文献大全
  • Molecular mechanisms of thrombus formation in ischemic stroke: novel insights and targets for treatment.

    abstract：:In ischemic stroke, treatment options are limited. Therapeutic thrombolysis is restricted to the first few hours after stroke, and the utility of current platelet aggregation inhibitors, including GPIIb/IIIa receptor antagonists, and anticoagulants is counterbalanced by the risk of intracerebral bleeding complications...

    journal_title：Blood

    pub_type： 杂志文章,评审

    doi：10.1182/blood-2008-04-144758

    authors： Stoll G,Kleinschnitz C,Nieswandt B

    更新日期：2008-11-01 00:00:00

  • Augmentation of antitumor effects by NK cell inhibitory receptor blockade in vitro and in vivo.

    abstract：:Subsets of natural killer (NK) cells are characterized by the expression of inhibitory and/or stimulatory receptors specific for major histocompatibility complex (MHC) class I determinants. In mice, these include the Ly49 family of molecules. One mechanism by which tumor cells may evade NK cell killing is by expressin...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood.v97.10.3132

    authors： Koh CY,Blazar BR,George T,Welniak LA,Capitini CM,Raziuddin A,Murphy WJ,Bennett M

    更新日期：2001-05-15 00:00:00

  • Inhibitory KIR/HLA incompatibility between sexual partners confers protection against HIV-1 transmission.

    abstract：:Killer immunoglobulin-like receptors (KIRs) regulate natural killer (NK) cells in a human leukocyte antigen (HLA)-dependent manner. KIR/HLA mismatched hematopoietic stem cell transplants induce alloreactive NK cells, which prevent leukemia relapse. Certain KIR/HLA combinations protect against HIV-1 infection, but the ...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood-2012-09-455352

    authors： Jennes W,Verheyden S,Mertens JW,Camara M,Seydi M,Dieye TN,Mboup S,Demanet C,Kestens L

    更新日期：2013-02-14 00:00:00

  • Caspase activation is required for nitric oxide-mediated, CD95(APO-1/Fas)-dependent and independent apoptosis in human neoplastic lymphoid cells.

    abstract：:Nitric oxide (NO), an important effector molecule involved in immune regulation and host defense, was shown to induce apoptosis in lymphoma cells. In the present report the NO donor glycerol trinitrate was found to induce apoptosis in Jurkat cells that are sensitive to CD95-mediated kill. In contrast, a CD95-resistant...

    journal_title：Blood

    pub_type： 杂志文章

    doi：

    authors： Chlichlia K,Peter ME,Rocha M,Scaffidi C,Bucur M,Krammer PH,Schirrmacher V,Umansky V

    更新日期：1998-06-01 00:00:00

  • Insulin-like growth factor 2 expressed in a novel fetal liver cell population is a growth factor for hematopoietic stem cells.

    abstract：:Hematopoietic stem cells (HSCs) undergo dramatic expansion during fetal liver development, but attempts to expand their numbers ex vivo have failed. We hypothesized that unidentified fetal liver cells produce growth factors that support HSC proliferation. Here we describe a novel population of CD3+ and Ter119- day-15 ...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood-2003-08-2955

    authors： Zhang CC,Lodish HF

    更新日期：2004-04-01 00:00:00

  • Cyclosporine v methotrexate for graft-v-host disease prevention in patients given marrow grafts for leukemia: long-term follow-up of three controlled trials.

    abstract：:One hundred seventy-nine patients with acute nonlymphoblastic leukemia in first remission (n = 75), chronic myelocytic leukemia in chronic or accelerated phase (n = 48) or leukemia in advanced stage (n = 56) were given HLA-identical marrow grafts and randomized to receive methotrexate or cyclosporine for prevention of...

    journal_title：Blood

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：

    authors： Storb R,Deeg HJ,Fisher L,Appelbaum F,Buckner CD,Bensinger W,Clift R,Doney K,Irle C,McGuffin R

    更新日期：1988-02-01 00:00:00

  • In vivo release and turnover of secreted platelet antiheparin proteins in rhesus monkey (Macaca mulatta).

    abstract：:Human and rhesus monkey platelets secrete at least two antiheparin proteins: platelet factor 4 (PF4) and low affinity platelet factor 4 (LA-PF4). Neither of these proteins showed species-related antigenic differences. As determined by radioimmunoassay, the levels of PF4 and LA-PF4 antigen per 10(9) monkey platelets am...

    journal_title：Blood

    pub_type： 杂志文章

    doi：

    authors： Musial J,Niewiarowski S,Edmunds LH Jr,Addonizio VP Jr,Nicolaou KC,Colman RW

    更新日期：1980-10-01 00:00:00

  • Detection of factor VIII inhibitors with the partial thromboplastin time.

    abstract：:Variations of the partial thromboplastin time (PTT) were tested to determine the best screening method for detection of inhibitors of factor VIII. Variables tested included the duration of preincubation of a mixture of patient plasma and factor VIII source (normal plasma), the ratio of the patient plasma to the normal...

    journal_title：Blood

    pub_type： 杂志文章

    doi：

    authors： Lossing TS,Kasper CK,Feinstein DI

    更新日期：1977-05-01 00:00:00

  • AKT inhibitor, GSK690693, induces growth inhibition and apoptosis in acute lymphoblastic leukemia cell lines.

    abstract：:The PI3K/AKT signaling is activated in various hematologic malignancies. We evaluated the effect of a novel, pan-AKT kinase inhibitor, GSK690693, on the proliferation of 112 cell lines representing different hematologic neoplasia. Fifty-five percent of all cell lines tested were sensitive to AKT inhibitor (EC(50)<1 mi...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood-2008-02-137737

    authors： Levy DS,Kahana JA,Kumar R

    更新日期：2009-02-19 00:00:00

  • Inactivation of the ATM gene in T-cell prolymphocytic leukemias.

    abstract：:T-cell prolymphocytic leukemia (T-PLL) is a rare form of mature leukemia that occurs both in adults as a sporadic disease and in younger patients suffering an hereditary condition, ataxia telangiectasia (AT). The ATM gene, located in the 11q22-23 chromosomal region, is consistently mutated in AT patients. The strong p...

    journal_title：Blood

    pub_type： 杂志文章

    doi：

    authors： Stoppa-Lyonnet D,Soulier J,Laugé A,Dastot H,Garand R,Sigaux F,Stern MH

    更新日期：1998-05-15 00:00:00

  • Activated protein C ameliorates chronic graft-versus-host disease by PAR1-dependent biased cell signaling on T cells.

    abstract：:Soluble thrombomodulin plasma concentrations are elevated in steroid-resistant graft-versus-host disease (GVHD), implying endothelial hypofunctioning for thrombomodulin-dependent generation of activated protein C's (APC) anticoagulant, anti-inflammatory, and antiapoptotic functions. Recombinant thrombomodulin or APC a...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood.2019001259

    authors： Sinha RK,Flynn R,Zaiken M,Paz K,Gavin AL,Nemazee D,Fernández JA,Xu X,Griffin JH,Blazar BR

    更新日期：2019-08-29 00:00:00

  • Production of interleukin-1 receptor antagonist and interleukin-1 beta by peripheral blood mononuclear cells is differentially regulated.

    abstract：:We studied the relationship between the production of the 23-Kd interleukin-1 receptor antagonist (IL-1ra) and IL-1 beta in cultures of human peripheral blood mononuclear cells (PBMC) using a specific radioimmunoassay for IL-1ra that had a sensitivity of 166 +/- 11 pg/mL. PBMC cultured without human serum made little ...

    journal_title：Blood

    pub_type： 杂志文章

    doi：

    authors： Poutsiaka DD,Clark BD,Vannier E,Dinarello CA

    更新日期：1991-09-01 00:00:00

  • One-year acyclovir prophylaxis for preventing varicella-zoster virus disease after hematopoietic cell transplantation: no evidence of rebound varicella-zoster virus disease after drug discontinuation.

    abstract：:No consensus exists on whether acyclovir prophylaxis should be given for varicella-zoster virus (VZV) prophylaxis after hematopoietic cell transplantation because of the concern of "rebound" VZV disease after discontinuation of prophylaxis. To determine whether rebound VZV disease is an important clinical problem and ...

    journal_title：Blood

    pub_type： 临床试验,杂志文章

    doi：10.1182/blood-2007-03-077644

    authors： Erard V,Guthrie KA,Varley C,Heugel J,Wald A,Flowers ME,Corey L,Boeckh M

    更新日期：2007-10-15 00:00:00

  • Interleukin-4 induces secretion of CSF for granulocytes and CSF for macrophages by peripheral blood monocytes.

    abstract：:T cells are known to interact cooperatively with monocytes to produce Colony-Stimulating Factors (CSF), although T cell-mediated signals leading to CSF secretion by monocytes are not completely understood. We have made use of Northern blot hybridization and specific bioassays to study the effects of the T cell product...

    journal_title：Blood

    pub_type： 杂志文章

    doi：

    authors： Wieser M,Bonifer R,Oster W,Lindemann A,Mertelsmann R,Herrmann F

    更新日期：1989-04-01 00:00:00

  • Impaired NK-cell migration in WAS/XLT patients: role of Cdc42/WASp pathway in the control of chemokine-induced beta2 integrin high-affinity state.

    abstract：:We analyzed the involvement of Wiskott-Aldrich syndrome protein (WASp), a critical regulator of actin cytoskeleton remodeling, in the control of natural killer (NK)-cell migration. NK cells derived from patients with Wiskott-Aldrich syndrome/X-linked thrombocytopenia (WAS/XLT), carrying different mutations in the WASP...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood-2009-07-235804

    authors： Stabile H,Carlino C,Mazza C,Giliani S,Morrone S,Notarangelo LD,Notarangelo LD,Santoni A,Gismondi A

    更新日期：2010-04-08 00:00:00

  • In vivo administration of stem cell factor to mice increases the absolute number of pluripotent hematopoietic stem cells.

    abstract：:We have examined the effects of administration of stem cell-factor (SCF) on the number and distribution of pluripotent hematopoietic stem cells (PHSC) in normal mice. Using the competitive repopulation assay we found that in vivo administration of SCF increases the absolute number of PHSC per mouse threefold. The incr...

    journal_title：Blood

    pub_type： 杂志文章

    doi：

    authors： Bodine DM,Seidel NE,Zsebo KM,Orlic D

    更新日期：1993-07-15 00:00:00

  • The c-kit receptor ligand functions as a mast cell chemoattractant.

    abstract：:Mast cells accumulate at sites of neovascularization, solid tumors, and many immune reactions. Such accumulation requires directed migration of mature mast cells or their precursors. The nature of the chemoattractants that regulate mast cell motility and the identity of the receptors that mediate the chemotactic respo...

    journal_title：Blood

    pub_type： 杂志文章

    doi：

    authors： Meininger CJ,Yano H,Rottapel R,Bernstein A,Zsebo KM,Zetter BR

    更新日期：1992-02-15 00:00:00

  • CD86 is expressed on murine hematopoietic stem cells and denotes lymphopoietic potential.

    abstract：:A unique subset of CD86(-) HSCs was previously discovered in mice that were old or chronically stimulated with lipopolysaccharide. Functionally defective HSCs were also present in those animals, and we now show that CD86(-) CD150(+) CD48(-) HSCs from normal adult mice are particularly poor at restoring the adaptive im...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood-2011-10-388736

    authors： Shimazu T,Iida R,Zhang Q,Welner RS,Medina KL,Alberola-Lla J,Kincade PW

    更新日期：2012-05-24 00:00:00

  • Fbw7γ-mediated degradation of KLF13 prevents RANTES expression in resting human but not murine T lymphocytes.

    abstract：:RANTES (CCL5) is a chemokine implicated in many human diseases. We previously showed that the transcription factor Kruppel-like factor 13 (KLF13) controls the late (3-5 days after activation) expression of RANTES in T lymphocytes and that KLF13 itself is translationally regulated through the 5'-untranslated region of ...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood-2012-03-415968

    authors： Kim DS,Zhang W,Millman SE,Hwang BJ,Kwon SJ,Clayberger C,Pagano M,Krensky AM

    更新日期：2012-08-23 00:00:00

  • Prognostic significance of baseline metabolic tumor volume in relapsed and refractory Hodgkin lymphoma.

    abstract：:Identification of prognostic factors for patients with relapsed/refractory Hodgkin lymphoma (HL) is essential for optimizing therapy with risk-adapted approaches. In our phase 2 study of positron emission tomography (PET)-adapted salvage therapy with brentuximab vedotin (BV) and augmented ifosfamide, carboplatin, and ...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood-2017-06-788877

    authors： Moskowitz AJ,Schöder H,Gavane S,Thoren KL,Fleisher M,Yahalom J,McCall SJ,Cadzin BR,Fox SY,Gerecitano J,Grewal R,Hamlin PA,Horwitz SM,Kumar A,Matasar M,Ni A,Noy A,Palomba ML,Perales MA,Portlock CS,Sauter C,Straus

    更新日期：2017-11-16 00:00:00

  • E47, IRF-4, and PU.1 synergize to induce B-cell-specific activation of the class II transactivator promoter III (CIITA-PIII).

    abstract：:In B cells, expression of CIITA and resulting major histocompatibility complex II (MHCII) is mediated exclusively by promoter III (CIITA-PIII) activation. Recent studies have established that CIITA-PIII also participates in the expression of CIITA in activated human T cells, dendritic cells, and monocytes. In this stu...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood-2004-03-0790

    authors： van der Stoep N,Quinten E,Marcondes Rezende M,van den Elsen PJ

    更新日期：2004-11-01 00:00:00

  • Heterogeneous prognostic impact of derivative chromosome 9 deletions in chronic myelogenous leukemia.

    abstract：:Derivative chromosome 9 deletions are seen in 10% to 15% of patients with chronic myelogenous leukemia and have been associated with a poor prognosis; however, no studies have been performed in the context of a randomized clinical trial. We developed a DNA-based deletion screen and investigated 339 chronic phase patie...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood-2007-02-074252

    authors： Kreil S,Pfirrmann M,Haferlach C,Waghorn K,Chase A,Hehlmann R,Reiter A,Hochhaus A,Cross NC,German Chronic Myelogenous Leukemia (CML) Study Group.

    更新日期：2007-08-15 00:00:00

  • How I treat infant leukemia.

    abstract：:Leukemia in infants is rare but generates tremendous interest due to its aggressive clinical presentation in a uniquely vulnerable host, its poor response to current therapies, and its fascinating biology. Increasingly, these biological insights are pointing the way toward novel therapeutic approaches. Using represent...

    journal_title：Blood

    pub_type： 杂志文章,随机对照试验

    doi：10.1182/blood-2018-04-785980

    authors： Brown P,Pieters R,Biondi A

    更新日期：2019-01-17 00:00:00

  • Human monocytes and neutrophils store transforming growth factor-alpha in a subpopulation of cytoplasmic granules.

    abstract：:Transforming growth factor-alpha (TGF-alpha) exerts several effects on target cells, such as neovascularization promotion and mitogenic signalling. Using immunoelectron microscopy, we show that monocytes and neutrophils, store TGF-alpha in cytoplasmic granules. In monocytes, TGF-alpha did not colocalize with component...

    journal_title：Blood

    pub_type： 杂志文章

    doi：

    authors： Calafat J,Janssen H,Ståhle-Bäckdahl M,Zuurbier AE,Knol EF,Egesten A

    更新日期：1997-08-01 00:00:00

  • Adaptive response of iron absorption to anemia, increased erythropoiesis, iron deficiency, and iron loading in beta2-microglobulin knockout mice.

    abstract：:Recently, a novel gene of the major histocompatibility complex (MHC) class I family, HFE (HLA-H), has been found to be mutated in a large proportion of hereditary hemochromatosis (HH) patients. Further support for a causative role of HFE in this disease comes from the observation that beta2-microglobulin knockout (bet...

    journal_title：Blood

    pub_type： 杂志文章

    doi：

    authors： Santos M,Clevers H,de Sousa M,Marx JJ

    更新日期：1998-04-15 00:00:00

  • Human NK cells at early stages of differentiation produce CXCL8 and express CD161 molecule that functions as an activating receptor.

    abstract：:Human natural killer (NK) cell development is a step-by-step process characterized by phenotypically identified stages. CD161 is a marker informative of the NK cell lineage commitment, whereas CD56, CD117, and CD94/NKG2A contribute to define discrete differentiation stages. In cells undergoing in vitro differentiation...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood-2011-09-379693

    authors： Montaldo E,Vitale C,Cottalasso F,Conte R,Glatzer T,Ambrosini P,Moretta L,Mingari MC

    更新日期：2012-04-26 00:00:00

  • Cyclin D1 overexpression is a favorable prognostic variable for newly diagnosed multiple myeloma patients treated with high-dose chemotherapy and single or double autologous transplantation.

    abstract：:We used a sensitive real-time reverse transcription-polymerase chain reaction assay to quantify cyclin D1 mRNA levels in bone marrow samples collected at diagnosis from 74 newly diagnosed multiple myeloma (MM) patients who were randomized to undergo either single or double autologous peripheral blood stem cell transpl...

    journal_title：Blood

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1182/blood-2002-12-3789

    authors： Soverini S,Cavo M,Cellini C,Terragna C,Zamagni E,Ruggeri D,Testoni N,Tosi P,De Vivo A,Amabile M,Grafone T,Ottaviani E,Giannini B,Cangini D,Bonifazi F,Neri A,Fabris S,Tura S,Baccarani M,Martinelli G

    更新日期：2003-09-01 00:00:00

  • Demonstration of Kaposi's sarcoma-associated herpes virus cyclin D homolog in cutaneous Kaposi's sarcoma by colorimetric in situ hybridization using a catalyzed signal amplification system.

    abstract：:Kaposi's sarcoma-associated herpes virus (KSHV)/human herpes virus 8 (HHV8) DNA sequences have been demonstrated in Kaposi's sarcoma (KS), as well as in some acquired immunodeficiency syndrome (AIDS)-related non-Hodgkin's lymphomas (NHL) and in multicentric Castleman's disease. Although KSHV DNA generally is abundant ...

    journal_title：Blood

    pub_type： 杂志文章

    doi：

    authors： Reed JA,Nador RG,Spaulding D,Tani Y,Cesarman E,Knowles DM

    更新日期：1998-05-15 00:00:00

  • Inhibitory receptors specific for MHC class I educate murine NK cells but not CD8αα intestinal intraepithelial T lymphocytes.

    abstract：:The engagement of inhibitory receptors specific for major histocompatibility complex class I (MHC-I) molecules educates natural killer (NK) cells, meaning the improvement of the response of activation receptors to subsequent stimulation. It is not known whether inhibitory MHC-I receptors educate only NK cells or wheth...

    journal_title：Blood

    pub_type： 杂志文章

    doi：10.1182/blood-2011-01-331124

    authors： Taveirne S,Filtjens J,Van Ammel E,De Colvenaer V,Kerre T,Taghon T,Vandekerckhove B,Plum J,Held W,Leclercq G

    更新日期：2011-07-14 00:00:00

  • Th1 and Th2 T-helper cells exert opposite regulatory effects on procoagulant activity and tissue factor production by human monocytes.

    abstract：:The role of T-cell subsets in the induction of tissue factor (TF) production by human monocytes in vitro was investigated. Mitogen stimulation enabled both unfractionated T cells and their CD4+ or CD8+ subsets to promote procoagulant activity (PCA). After mitogen or antigen activation, all seven T-cell clones with Th1...

    journal_title：Blood

    pub_type： 杂志文章

    doi：

    authors： Del Prete G,De Carli M,Lammel RM,D'Elios MM,Daniel KC,Giusti B,Abbate R,Romagnani S

    更新日期：1995-07-01 00:00:00