Demonstration of Kaposi's sarcoma-associated herpes virus cyclin D homolog in cutaneous Kaposi's sarcoma by colorimetric in situ hybridization using a catalyzed signal amplification system.

Abstract:

:Kaposi's sarcoma-associated herpes virus (KSHV)/human herpes virus 8 (HHV8) DNA sequences have been demonstrated in Kaposi's sarcoma (KS), as well as in some acquired immunodeficiency syndrome (AIDS)-related non-Hodgkin's lymphomas (NHL) and in multicentric Castleman's disease. Although KSHV DNA generally is abundant in KSHV-associated lymphomas, few copies of the virus are present in KS, a property that confounds detection by in situ methods. Previous in situ studies, which identified KSHV in lesions of KS, relied on the use of polymerase chain reaction (PCR) to amplify target DNA sequences before in situ hybridization (ISH) for localization or used ISH with radioactively-labeled probes to obtain adequate levels of detection sensitivity. In this study, a novel nonisotopic nucleic acid ISH method using catalyzed signal amplification and colorimetric detection without PCR-dependent target amplification was used to identify KSHV-specific sequences. The level of sensitivity was increased further by using a probe that detects viral cyclin D homolog transcripts, which are expressed at significant levels during latent viral infection. Thirty cutaneous lesions of KS (25 AIDS-related and five classical European type) were evaluated. AIDS-related NHL and cell lines derived from patients with AIDS-related NHL, all of which were known to harbor KSHV by Southern blot analysis, were used as positive controls. NHL and benign cutaneous vascular lesions not associated with AIDS were used as negative controls. For each of the 30 KS lesions studied, hybridization signals were detected in most of the spindle cells surrounding the atypical slit-like vascular channels and also were detected in some endothelial cells in well-formed blood vessels in the perilesional dermis. Plaque and nodular lesions generally contained more labeled cells than did early patch lesions. All AIDS-related NHL and cell lines contained KSHV-specific sequences; however, the non-AIDS-related NHLs and benign vascular lesions were negative. These results confirm the presence of KSHV sequences in cutaneous KS and provide in situ evidence of infection by this virus in early patch-stage lesions. This study also defines the in situ expression of the KSHV cyclin D homolog viral oncogene in cutaneous KS. The use of this sensitive nonisotopic ISH method should allow detection of other KSHV-specific gene products, further defining the pathobiology of this virus.

journal_name

Blood

journal_title

Blood

authors

Reed JA,Nador RG,Spaulding D,Tani Y,Cesarman E,Knowles DM

subject

Has Abstract

pub_date

1998-05-15 00:00:00

pages

3825-32

issue

10

eissn

0006-4971

issn

1528-0020

journal_volume

91

pub_type

杂志文章

相关文献

BLOOD文献大全
  • Decreased incidence of marrow graft rejection in patients with severe aplastic anemia: changing impact of risk factors.

    abstract::Patients with severe aplastic anemia were conditioned with cyclophosphamide (200 mg/kg) and given marrow grafts from HLA-identical family members. Among 233 patients transplanted, 225 survived greater than or equal to 14 days and could be evaluated for engraftment. Forty-four of the 225 rejected their graft; 33 of the...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Deeg HJ,Self S,Storb R,Doney K,Appelbaum FR,Witherspoon RP,Sullivan KM,Sheehan K,Sanders J,Mickelson E

    更新日期:1986-12-01 00:00:00

  • How I treat infant leukemia.

    abstract::Leukemia in infants is rare but generates tremendous interest due to its aggressive clinical presentation in a uniquely vulnerable host, its poor response to current therapies, and its fascinating biology. Increasingly, these biological insights are pointing the way toward novel therapeutic approaches. Using represent...

    journal_title:Blood

    pub_type: 杂志文章,随机对照试验

    doi:10.1182/blood-2018-04-785980

    authors: Brown P,Pieters R,Biondi A

    更新日期:2019-01-17 00:00:00

  • Live imaging of Runx1 expression in the dorsal aorta tracks the emergence of blood progenitors from endothelial cells.

    abstract::Blood cells of an adult vertebrate are continuously generated by hematopoietic stem cells (HSCs) that originate during embryonic life within the aorta-gonad-mesonephros region. There is now compelling in vivo evidence that HSCs are generated from aortic endothelial cells and that this process is critically regulated b...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2010-01-264382

    authors: Lam EY,Hall CJ,Crosier PS,Crosier KE,Flores MV

    更新日期:2010-08-12 00:00:00

  • Keratinocyte growth factor administered before conditioning ameliorates graft-versus-host disease after allogeneic bone marrow transplantation in mice.

    abstract::Keratinocyte growth factor (KGF) is important in tissue repair and wound healing and its administration can abrogate chemical- and radiation-induced tissue damage in rodents. We investigated KGF as a therapeutic agent for the prevention of graft-versus-host disease (GVHD)-induced tissue damage, morbidity, and mortalit...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Panoskaltsis-Mortari A,Lacey DL,Vallera DA,Blazar BR

    更新日期:1998-11-15 00:00:00

  • Lymphokine(s) from isolated T lymphocyte subpopulations support multilineage hematopoietic colony and megakaryocytic colony formation.

    abstract::Conditioned medium derived from peripheral mononuclear low-density cells stimulated with phytohemagglutinin (PHA) supports the growth of noncommitted hematopoietic progenitors from marrow and peripheral blood cells. These immature progenitors (CFU-GEMM) can be identified in culture as multilineage hematopoietic coloni...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Kanz L,Löhr GW,Fauser AA

    更新日期:1986-11-01 00:00:00

  • Gamma heavy chain disease: clinical aspects and characterization of a deleted, noncovalently linked gamma1 heavy chain dimer (BAZ).

    abstract::This report describes the clinical and immunoglobulin features of a patient with gamma heavy chain disease (HCD), who presented with a clinical picture suggestive of an underlying malignancy rather than the usual picture of lymphoma or granulomatous disease. A unique clinical feature was the nearly total replacement o...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Faguet GB,Barton BP,Smith LL,Garver FA

    更新日期:1977-04-01 00:00:00

  • HIV-1 reprograms the migration of macrophages.

    abstract::Macrophages are motile leukocytes, targeted by HIV-1, thought to play a critical role in host dissemination of the virus. However, whether infection impacts their migration capacity remains unknown. We show that 2-dimensional migration and the 3-dimensional (3D) amoeboid migration mode of HIV-1-infected human monocyte...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2014-08-596775

    authors: Vérollet C,Souriant S,Bonnaud E,Jolicoeur P,Raynaud-Messina B,Kinnaer C,Fourquaux I,Imle A,Benichou S,Fackler OT,Poincloux R,Maridonneau-Parini I

    更新日期:2015-03-05 00:00:00

  • Peroxiredoxin 2 functions as a noncatalytic scavenger of low-level hydrogen peroxide in the erythrocyte.

    abstract::Peroxiredoxin 2 (Prx2), a thiol-dependent peroxidase, is the third most abundant protein in the erythrocyte, and its absence in knock-out mice gives rise to hemolytic anemia. We have found that in human erythrocytes, Prx2 was extremely sensitive to oxidation by H(2)O(2), as dimerization was observed after exposure of ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2006-09-048728

    authors: Low FM,Hampton MB,Peskin AV,Winterbourn CC

    更新日期:2007-03-15 00:00:00

  • Lineage promiscuity in hemopoietic differentiation and leukemia.

    abstract::An increasing number of reports document instances in which individual leukemic cells coexpress markers normally believed to be restricted to a single lineage. This has been interpreted by McCulloch and colleagues as aberrant programming or lineage infidelity and contrasts with earlier suggestions that lineage fidelit...

    journal_title:Blood

    pub_type: 杂志文章,评审

    doi:

    authors: Greaves MF,Chan LC,Furley AJ,Watt SM,Molgaard HV

    更新日期:1986-01-01 00:00:00

  • High-dose therapy and autologous peripheral blood stem cell transplantation for patients with lymphoma.

    abstract::Forty patients with refractory Hodgkin's disease (24 patients) or non-Hodgkin's lymphoma (16 patients) who were considered for high-dose therapy but not for autologous bone marrow transplantation (ABMT) due to BM metastases, previous pelvic irradiation, a history of marrow involvement by tumor or hypocellular marrow i...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Kessinger A,Armitage JO,Smith DM,Landmark JD,Bierman PJ,Weisenburger DD

    更新日期:1989-09-01 00:00:00

  • Long-term immune reconstitution and outcome after HLA-nonidentical T-cell-depleted bone marrow transplantation for severe combined immunodeficiency: a European retrospective study of 116 patients.

    abstract::We have performed a retrospective analysis of the development of T- and B-cell functions after HLA-nonidentical T-cell-depleted bone marrow transplantation (BMT) performed in 193 patients with severe combined immunodeficiency (SCID) at 18 European centers between December 1982 and December 31, 1993. One hundred sixtee...

    journal_title:Blood

    pub_type: 杂志文章,多中心研究

    doi:

    authors: Haddad E,Landais P,Friedrich W,Gerritsen B,Cavazzana-Calvo M,Morgan G,Bertrand Y,Fasth A,Porta F,Cant A,Espanol T,Müller S,Veys P,Vossen J,Fischer A

    更新日期:1998-05-15 00:00:00

  • Identification of a permissible HLA mismatch in hematopoietic stem cell transplantation.

    abstract::In subjects mismatched in the HLA alleles C*03:03/C*03:04 no allogeneic cytotoxic T-lymphocyte responses are detected in vitro. Hematopoietic stem cell transplantation (HSCT) with unrelated donors (UDs) showed no association between the HLA-C allele mismatches (CAMMs) and adverse outcomes; antigen mismatches at this a...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2013-10-532671

    authors: Fernandez-Viña MA,Wang T,Lee SJ,Haagenson M,Aljurf M,Askar M,Battiwalla M,Baxter-Lowe LA,Gajewski J,Jakubowski AA,Marino S,Oudshoorn M,Marsh SG,Petersdorf EW,Schultz K,Turner EV,Waller EK,Woolfrey A,Umejiego J,Spell

    更新日期:2014-02-20 00:00:00

  • Thrombopoietin therapy increases platelet yields in healthy platelet donors.

    abstract::The recombinant thrombopoietins have been shown to be effective stimulators of platelet production in cancer patients. It was therefore of interest to determine if one of these, pegylated recombinant human megakaryocyte growth and development factor (PEG-rHuMGDF), could be used to increase platelet counts and conseque...

    journal_title:Blood

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1182/blood.v98.5.1339

    authors: Kuter DJ,Goodnough LT,Romo J,DiPersio J,Peterson R,Tomita D,Sheridan W,McCullough J

    更新日期:2001-09-01 00:00:00

  • Role of additional chromosomal changes in the prognostic value of t(4;14) and del(17p) in multiple myeloma: the IFM experience.

    abstract::In multiple myeloma, cytogenetic changes are important predictors of patient outcome. In this setting, the most important changes are deletion 17p, del(17p), and translocation of chromosomes 4 and 14, t(4;14), conferring a poor outcome. However, a certain degree of heterogeneity is observed in the survival of these hi...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2014-07-587964

    authors: Hebraud B,Magrangeas F,Cleynen A,Lauwers-Cances V,Chretien ML,Hulin C,Leleu X,Yon E,Marit G,Karlin L,Roussel M,Stoppa AM,Belhadj K,Voillat L,Garderet L,Macro M,Caillot D,Mohty M,Facon T,Moreau P,Attal M,Munshi N

    更新日期:2015-03-26 00:00:00

  • Notch-dependent control of myelopoiesis is regulated by fucosylation.

    abstract::Cell-cell contact-dependent mechanisms that modulate proliferation and/or differentiation in the context of hematopoiesis include mechanisms characteristic of the interactions between members of the Notch family of signal transduction molecules and their ligands. Whereas Notch family members and their ligands clearly ...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2007-11-115204

    authors: Zhou L,Li LW,Yan Q,Petryniak B,Man Y,Su C,Shim J,Chervin S,Lowe JB

    更新日期:2008-07-15 00:00:00

  • Immunostimulatory conventional dendritic cells evolve into regulatory macrophage-like cells.

    abstract::Dendritic cell (DC) homeostasis in peripheral tissues reflect a balance between DC generation, migration, and death. The current model of DC ontogeny indicates that pre-cDCs are committed to become terminal conventional DCs (cDCs). Here, we report the unexpected finding that proliferating immunostimulatory CD11c(+) MH...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2011-11-392894

    authors: Diao J,Mikhailova A,Tang M,Gu H,Zhao J,Cattral MS

    更新日期:2012-05-24 00:00:00

  • Human CD34+ fetal liver stem cells differentiate to T cells in a mouse thymic microenvironment.

    abstract::Hematopoietic stem cells differentiate in the thymus to T cells along precisely defined intermediates. This process is thymic epithelium dependent and involves cytokines and cell-cell interactions between thymic stroma and T-cell precursors. Here we report that highly purified human CD34++ fetal liver stem cells diffe...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Plum J,De Smedt M,Defresne MP,Leclercq G,Vandekerckhove B

    更新日期:1994-09-01 00:00:00

  • Serum ferritin as risk factor for sinusoidal obstruction syndrome of the liver in patients undergoing hematopoietic stem cell transplantation.

    abstract::Hepatic sinusoidal obstruction syndrome (SOS) is a serious complication in hematopoietic stem cell transplant (HSCT) recipients. To determine the impact of pretransplantation hyperferritinemia on the risk of SOS after HSC transplantation, we retrospectively studied 427 HSCT recipients (179 autologous and 248 allogenei...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2009-03-212282

    authors: Maradei SC,Maiolino A,de Azevedo AM,Colares M,Bouzas LF,Nucci M

    更新日期:2009-08-06 00:00:00

  • Telomere length correlates with histopathogenesis according to the germinal center in mature B-cell lymphoproliferative disorders.

    abstract::In this study we investigated telomere restriction fragment (TRF) length in a panel of mature B-cell lymphoproliferative disorders (MBCLDs) and correlated this parameter with histology and histopathogenesis in relation to the germinal center (GC). We assessed 123 MBCLD samples containing 80% or more tumor cells. TRF l...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2003-12-4412

    authors: Ladetto M,Compagno M,Ricca I,Pagano M,Rocci A,Astolfi M,Drandi D,di Celle PF,Dell'Aquila M,Mantoan B,Vallet S,Pagliano G,De Marco F,Francese R,Santo L,Cuttica A,Marinone C,Boccadoro M,Tarella C

    更新日期:2004-06-15 00:00:00

  • Maea expressed by macrophages, but not erythroblasts, maintains postnatal murine bone marrow erythroblastic islands.

    abstract::The erythroblastic island (EI), formed by a central macrophage and developing erythroblasts (EBs), was first described decades ago and was recently shown to play an in vivo role in homeostatic and pathological erythropoiesis. The exact molecular mechanisms, however, mediating the interactions between macrophages and E...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2018-11-888180

    authors: Wei Q,Boulais PE,Zhang D,Pinho S,Tanaka M,Frenette PS

    更新日期:2019-03-14 00:00:00

  • Characterization of murine CD34, a marker for hematopoietic progenitor and stem cells.

    abstract::CD34 is expressed on human hematopoietic stem and progenitor cells, and its clinical usefulness for the purification of stem cells has been well established. However, a similar pattern of expression for murine CD34 (mCD34) has not yet been determined. Two polyclonal anti-mCD34 antibodies that specifically recognize bo...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Krause DS,Ito T,Fackler MJ,Smith OM,Collector MI,Sharkis SJ,May WS

    更新日期:1994-08-01 00:00:00

  • Efficient HIV-1 transmission from macrophages to T cells across transient virological synapses.

    abstract::Macrophages are reservoirs of HIV-1 infection, proposed to transmit virus to CD4(+) T cells, the primary target of the virus. Here we report that human monocyte-derived macrophages (MDMs) rapidly spread HIV-1 to autologous CD4(+) T cells resulting in productive infection. Transmission takes place across transient adhe...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2007-12-130070

    authors: Groot F,Welsch S,Sattentau QJ

    更新日期:2008-05-01 00:00:00

  • Long-term outcomes after autologous stem cell transplantation for patients with POEMS syndrome (osteosclerotic myeloma): a single-center experience.

    abstract::The POEMS syndrome (polyradiculoneuropathy, organomegaly, multiple endocrinopathies, monoclonal protein, skin changes) is a rare disease associated with a plasma cell dyscrasia. Patients with disseminated POEMS can be treated with high-dose therapy and autologous stem cell transplantation (ASCT). While clinical improv...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2012-04-423178

    authors: D'Souza A,Lacy M,Gertz M,Kumar S,Buadi F,Hayman S,Dingli D,Zeldenrust S,Kyle R,Ansell S,Inwards D,Johnston P,Micallef I,Porrata L,Litzow M,Gastineau D,Hogan W,Dispenzieri A

    更新日期:2012-07-05 00:00:00

  • FCR achieves long-term durable remissions in patients with IGHV-mutated CLL.

    abstract::In chronic lymphocytic leukemia (CLL) patients with mutated IGHV, 3 recent studies have demonstrated prolonged progression-free survival (PFS) after treatment with fludarabine-cyclophosphamide-rituximab (FCR) chemoimmunotherapy. We performed a systematic review to assess the benefit of FCR for patients with CLL and id...

    journal_title:Blood

    pub_type: 杂志文章,评审

    doi:10.1182/blood-2017-07-731588

    authors: Chai-Adisaksopha C,Brown JR

    更新日期:2017-11-23 00:00:00

  • Mechanisms of organelle transport and capture along proplatelets during platelet production.

    abstract::Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Platelet packaging and release concludes at the tips of each proplatelet. Essential in this process is the distribution of organelles and platelet-specific granules i...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2005-06-2206

    authors: Richardson JL,Shivdasani RA,Boers C,Hartwig JH,Italiano JE Jr

    更新日期:2005-12-15 00:00:00

  • JMML and RALD (Ras-associated autoimmune leukoproliferative disorder): common genetic etiology yet clinically distinct entities.

    abstract::Ras-associated autoimmune leukoproliferative disorder (RALD) is a chronic, nonmalignant condition that presents with persistent monocytosis and is often associated with leukocytosis, lymphoproliferation, and autoimmune phenomena. RALD has clinical and laboratory features that overlap with those of juvenile myelomonocy...

    journal_title:Blood

    pub_type: 杂志文章,评审

    doi:10.1182/blood-2014-11-567917

    authors: Calvo KR,Price S,Braylan RC,Oliveira JB,Lenardo M,Fleisher TA,Rao VK

    更新日期:2015-04-30 00:00:00

  • Phosphorylation of protein 4.1 in Plasmodium falciparum-infected human red blood cells.

    abstract::The composition of the erythrocyte plasma membrane is extensively modified during the intracellular growth of the malaria parasite Plasmodium falciparum. It has been previously shown that an 80-kD phosphoprotein is associated with the plasma membrane of human red blood cells (RBCs) infected with trophozoite/schizont s...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Chishti AH,Maalouf GJ,Marfatia S,Palek J,Wang W,Fisher D,Liu SC

    更新日期:1994-06-01 00:00:00

  • Human platelet cGI-PDE: expression in yeast and localization of the catalytic domain by deletion mutagenesis.

    abstract::Cyclic adenosine monophosphate (cAMP) is an important modulator of platelet responses to agonists. Cyclic nucleotide phosphodiesterase (PDE) controls intracellular cAMP concentrations by hydrolyzing it to AMP. The major PDE activity in platelets is PDE3A (cyclic guanosine monophosphate [cGMP]-inhibited PDE). To obtain...

    journal_title:Blood

    pub_type: 杂志文章

    doi:

    authors: Cheung PP,Xu H,McLaughlin MM,Ghazaleh FA,Livi GP,Colman RW

    更新日期:1996-08-15 00:00:00

  • Specific killing of multiple myeloma cells by (-)-epigallocatechin-3-gallate extracted from green tea: biologic activity and therapeutic implications.

    abstract::Epigallocatechin-3-gallate (EGCG), a polyphenol extracted from green tea, is an antioxidant with chemopreventive and chemotherapeutic actions. Based on its ability to modulate growth factor-mediated cell proliferation, we evaluated its efficacy in multiple myeloma (MM). EGCG induced both dose- and time-dependent growt...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood-2006-05-022814

    authors: Shammas MA,Neri P,Koley H,Batchu RB,Bertheau RC,Munshi V,Prabhala R,Fulciniti M,Tai YT,Treon SP,Goyal RK,Anderson KC,Munshi NC

    更新日期:2006-10-15 00:00:00

  • Asymptomatic primary Epstein-Barr virus infection occurs in the absence of blood T-cell repertoire perturbations despite high levels of systemic viral load.

    abstract::Primary infection with the human herpesvirus, Epstein-Barr virus (EBV), may result in subclinical seroconversion or may appear as infectious mononucleosis (IM), a lymphoproliferative disease of variable severity. Why primary infection manifests differently between patients is unknown, and, given the difficulties in id...

    journal_title:Blood

    pub_type: 杂志文章

    doi:10.1182/blood.v98.13.3739

    authors: Silins SL,Sherritt MA,Silleri JM,Cross SM,Elliott SL,Bharadwaj M,Le TT,Morrison LE,Khanna R,Moss DJ,Suhrbier A,Misko IS

    更新日期:2001-12-15 00:00:00