Abstract：
INTRODUCTION:In the Olympus uric acid procedure, uric acid is converted by uricase to allantoin and hydrogen peroxide, which is reacted in a Trinder reaction to produce a chromophore read bichromatically at 520 and 660 nm. Repeated difficulty was encountered in obtaining uric acid results on samples from myeloma patients with known IgM paraproteins. Large absorbances in sample blanks were due to a visible precipitation observed in the reaction cuvettes. OBJECTIVE:To alter the Olympus method (OM) to eliminate the interference by IgM, and to verify the modified method (MM). METHODS:Dilution of the sample blank by saline was substituted for water in the MM, with small alterations in the reaction timing sequence necessary to accommodate the instrument requirements. RESULTS:A comparison of uric acid results obtained from nonmyeloma patient samples using the OM and the MM showed a good correlation (r = 0.970), and no statistical difference between the two means using a paired t-test. A similar comparison performed using the samples containing IgA and IgG paraproteins also revealed a good correlation (r = 0.981), and no statistical difference between the two means. Results on IgM containing specimens were assessed indirectly because the samples could not be assayed with the OM. First, removal of detectable levels of proteins using a 20% TCA solution did not affect the measurement of uric acid. Second, protein-free supernatants from IgM containing samples were measured by the OM and compared with the corresponding serum samples measured by the MM. There was good correlation between the two methods (r = 0.945), and no statistical difference between the means using a paired t-test. CONCLUSION:The modified method is satisfactory for routine analysis of samples, including those with IgM paraproteins.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Langman LJ,Allen LC,Romaschin ADdoi
10.1016/s0009-9120(98)00064-2subject
Has Abstractpub_date
1998-10-01 00:00:00pages
517-21issue
7eissn
0009-9120issn
1873-2933pii
S0009-9120(98)00064-2journal_volume
31pub_type
杂志文章abstract：BACKGROUND:In standard pre-analytical conditions, an isolation step is required for circulating tumor DNA (ctDNA) analysis. The need for this step remains unclear with the development of ultrasensitive detection technologies such as digital PCR (dPCR). The aim of our study was to evaluate the ctDNA detection by dPCR pl...
journal_title：Clinical biochemistry
pub_type： 杂志文章
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更新日期：2017-11-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2013.07.014
更新日期：2013-11-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 临床试验,杂志文章,多中心研究
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更新日期：2019-01-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2008.09.113
更新日期：2009-01-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
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更新日期：2009-03-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2012.12.021
更新日期：2013-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0009-9120(88)80084-5
更新日期：1988-10-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(79)80136-8
更新日期：1979-08-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
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更新日期：2008-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2017-03-01 00:00:00
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doi：10.1016/j.clinbiochem.2012.07.086
更新日期：2012-10-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/0009-9120(91)90357-k
更新日期：1991-02-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0009-9120(86)80030-3
更新日期：1986-08-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2016.08.006
更新日期：2016-11-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2014.05.064
更新日期：2014-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/s0009-9120(76)80040-9
更新日期：1976-06-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(98)00020-4
更新日期：1998-06-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2015.01.015
更新日期：2015-05-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2010.04.001
更新日期：2010-06-01 00:00:00
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doi：10.1016/j.clinbiochem.2007.03.009
更新日期：2007-07-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2012.06.004
更新日期：2012-11-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2020.12.011
更新日期：2021-01-09 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
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更新日期：2012-02-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2015.03.007
更新日期：2015-06-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2011.08.1148
更新日期：2011-12-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(91)80018-x
更新日期：1991-06-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2014.01.011
更新日期：2014-05-01 00:00:00
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journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/0009-9120(91)80003-l
更新日期：1991-08-01 00:00:00
abstract：OBJECTIVES:To investigate the possible association between functional variant Ala-9Val in the MnSOD gene and asthma in the case-control study comprising 626 Caucasian subjects. METHODS:MnSOD genotypes were determined by PCR with subsequent restriction analysis by the BsaWI enzyme. RESULTS:Significant differences in a...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2005.12.001
更新日期：2006-03-01 00:00:00
