Abstract：
:The solid-phase radioimmunoassay procedures for serum digoxin and cortisol with the Concept-4 were evaluated for specificity, correlation with other radioimmunoassay procedures, parallelism, recovery, carryover, and precision. 1. The detection limits of the digoxin and cortisol assays were 10 pg/tube and 40 pg/tube, respectively. These detections correspond to serum concentrations of 0.25 micrograms/liter and 20 micrograms/liter, respectively, for digoxin and cortisol. 2. Specificity of the digoxin assay was characterized by a 18.3% cross reaction with digitoxin. The anti-cortisol with 11-deoxycortisol, cross reaction with 11-deoxycortisol. 3. The values obtained with the Concept-4 for both procedures demonstrated acceptable correlations with other existing RIA procedures. 4. Dilution studies conducted to determine the parallelism of the 2 assays indicated that both assays demonstrated excellent proportionality between the volume of specimen and the quantity of analyte measured. 5. Analytical recoveries of digoxin and cortisol added to the individual assays average 95% and 101%, respectively. 6. Carryover studies indicated a routine sample-to-sample interaction of less than 0.05% for both assays. 7. The digoxin assay routinely demonstrated intra- and inter-assay variations of less than 7.4% and less than 10.0%, respectively. The precision data for cortisol indicated intra- and inter-assay variations of less than 6.7% and less than 9.1%, respectively.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Walsh PR,Abarca SD,Wang MCdoi
10.1016/s0009-9120(79)80136-8subject
Has Abstractpub_date
1979-08-01 00:00:00pages
118-22issue
4eissn
0009-9120issn
1873-2933pii
S0009-9120(79)80136-8journal_volume
12pub_type
杂志文章abstract：OBJECTIVES:Leptin is a multifunctional polypeptide produced primarily by adipocytes and associated with the occurrence of obesity and insulin resistance. Tumor necrosis factor-alpha (TNF-alpha), a polypeptide cytokine produced primarily by mononuclear phagocytes, plays a key role in the initiation of the inflammatory r...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(02)00314-4
更新日期：2002-06-01 00:00:00
abstract：:Steroids play an important role in sperm production and quality. These hormones have been extensively studied in blood, but poorly investigated in semen. The purpose of our study was to evaluate the relationship between sperm quality and steroid profiles in blood and semen in a small cohort of young Swiss men. Another...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2018.03.008
更新日期：2018-12-01 00:00:00
abstract：:We evaluated two chemical methods for quantifying mannitol in serum, based on the oxidation of mannitol by periodate, and measurement of the formaldehyde formed with chromotropic acid (colorimetry) or acetylacetone (fluorometry). We found interference in these methods by serum glycerol. Additionally, a high-performanc...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(92)90120-h
更新日期：1992-12-01 00:00:00
abstract：OBJECTIVES:Biallelic mutations in the ARG1 gene result in an uncommon autosomal recessive inborn defect of the urea cycle known as hyperargininemia (OMIM #207800). ARG1 splicing mutations are not reported often, and they are probably related to a more severe phenotype than missense mutations. In this article, we descri...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2015.07.015
更新日期：2015-12-01 00:00:00
abstract：OBJECTIVE:We performed a side-by-side comparison between three stain-then-lyse commercially available methods (Ortho-mune Lysing solution, FACS lysing solution and ImmunoPrep reagent system) and a lyse-then-stain method using hypotonic NH4Cl. The major difference between these methods is that only in the latter the ali...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(98)00011-3
更新日期：1998-04-01 00:00:00
abstract：:Ischemic heart disease is the primary cause of death worldwide. The pathophysiological process of cardiovascular diseases is linked to atheromatous plaque formation, while plaque rupture releases thrombogenic elements, which lead to activation of platelets, blood clotting and formation of thrombi. Platelet inhibitors ...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2014.03.005
更新日期：2014-05-01 00:00:00
abstract：OBJECTIVE:To evaluate a novel surface plasmon resonance (SPR) system for the direct measurement of tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva. DESIGN AND METHODS:We measured the presence of the tumor marker carbohydrate antigen 15-3 (CA15-3) in human saliva using 2 different surface plasmon resona...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2012.09.001
更新日期：2012-12-01 00:00:00
abstract：OBJECTIVES:Investigate the interference of Virox® disinfectant wipes containing hydrogen peroxide with Lifescan SureStep Flexx® glucose meter results. DESIGN AND METHODS:Glucose measurements of control and whole blood samples were performed before and after cleaning with Virox® wipes to reveal the kinetics of this int...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2010.09.012
更新日期：2010-12-01 00:00:00
abstract：BACKGROUND:With the advent of the new high-sensitivity troponin assays, it is becoming critical to measure troponin accurately to low concentrations. To ensure assay performance is acceptable, appropriate QC must be run. METHODS:In addition to the routine use of commercial QC material, we prepared pools of human QC ma...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2018.05.007
更新日期：2018-08-01 00:00:00
abstract：OBJECTIVES:The purpose of this study was to measure the extent of lipid peroxidation and the status of antioxidants in patients with Hodgkin's disease. DESIGN AND METHODS:Glutathione peroxidase (GPX) and superoxide dismutase (SOD) activities, and malondialdehyde (MDA), selenium, zinc and copper content have been measu...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(00)00062-x
更新日期：2000-04-01 00:00:00
abstract：BACKGROUND:While investigating estrogen response element (ERE) binding properties of human estrogen receptor-α (hERα) in breast cancer cytosols, other ERE-binding proteins (ERE-BP) were observed. DESIGN AND METHODS:Recognition properties of ERE-BP were evaluated by electrophoretic mobility shift assays (EMSA) with ERE...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2013.07.005
更新日期：2013-11-01 00:00:00
abstract：OBJECTIVES:The use of iodinated contrast media has grown in popularity in the past two decades, but relatively little attention has been paid to the possible interferential effects of contrast media on laboratory test results. Herein, we investigate medical contrast media interference with routine chemistry results obt...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2017.02.023
更新日期：2017-08-01 00:00:00
abstract：OBJECTIVES:Mucopolysaccharidoses (MPSs) are a group of rare, inherited metabolic disorders which result from the lack of one of the lysosomal enzymes responsible for the degradation of glycosaminoglycans. Early recognition of MPS is important as it enables prompt implementation of enzyme replacement therapy (ERT). Dipe...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2015.11.011
更新日期：2016-04-01 00:00:00
abstract：:An immunosuppressive substance was isolated from ascitic fluids of patients with advanced colon cancer by means of ammonium sulphate precipitation and preparative isoelectric focusing. This was a glycoprotein with an isoelectric point of pH 2.7-3.3, a molecular weight of about 52,000, and a sedimentation coefficient o...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(87)80118-2
更新日期：1987-06-01 00:00:00
abstract：OBJECTIVES:The aim of the study was to examine the long-term effect of commonly used whole soy foods (soy flour) and purified daidzein (one major isoflavone and the precursor of equol) on renal function among prehypertensive postmenopausal women who are also equol producers, a population most likely to benefit from soy...
journal_title：Clinical biochemistry
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.clinbiochem.2014.05.054
更新日期：2014-09-01 00:00:00
abstract：:Creatine kinase (CK) isoenzymes were determined on 15 patients undergoing open heart surgery with bypass. The study was performed to examine the relationship between the hypothermia under which the surgery is conducted and the appearance of CK isoenzymes in the serum both during and after surgery. All patients showed ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(82)96822-9
更新日期：1982-12-01 00:00:00
abstract：:The concentrations in serum of total and ionised calcium, phosphate, magnesium, albumin and alkaline phosphatase activity were measured in patients when hyperthyroid and again when euthyroid. Significant declines in the mean values of ionised calcium, phosphate and alkaline phosphatase activity and significant increas...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(89)80035-9
更新日期：1989-10-01 00:00:00
abstract：BACKGROUND:Vitamin D (25-OHD) physiological functions have been expanded beyond traditional bone health, increasing the importance of its estimation in Laboratory Medicine, which renders validation of available methods mandatory. AIMS AND METHODS:We evaluated some preanalytical and analytical aspects of 25-OHD determi...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2012.08.003
更新日期：2012-12-01 00:00:00
abstract：:Iodine deficiency (ID) is associated with increased prevalence of goiter, increased risk for neurodevelopmental disorders, and is the world's leading cause of intellectual deficits. Iodine nutritional status of a population is assessed by measurements of urinary iodine concentrations which are also used to define, ind...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/s0009-9120(02)00406-x
更新日期：2002-11-01 00:00:00
abstract：OBJECTIVE:To externally validate a clinical decision rule incorporating heart fatty acid binding protein (h-FABP), high-sensitivity troponin (hs-cTn) and electrocardiogram (ECG) for the detection of acute myocardial infarction (AMI) on presentation to the Emergency Department. We also investigated whether this clinical...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2017.10.001
更新日期：2018-02-01 00:00:00
abstract：OBJECTIVES:Vitamin B(12), or B(12), is an essential nutrient for humans, and its deficiency is a public health problem, especially in elderly population. Around 30% of circulating total B(12) levels are attached to transcobalamin II (TCN2), being referred as holotranscobalamin (holo-TC), and representing the biological...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2010.01.015
更新日期：2010-05-01 00:00:00
abstract：:This article reviews some of the important determinants of variation in drug disposition such as age, gender, body weight, diet, environmental influences, drug - protein interactions, compliance, drug - drug interactions, endogenous substances, disease states, circadian variation and genetics. ...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/s0009-9120(86)80050-9
更新日期：1986-04-01 00:00:00
abstract：:We describe a fast and efficient method for the isolation of drugs from blood serum by use of the DU PONT PREP I Automated Sample Processor and octadecyl bonded phase columns. Drugs are selectively adsorbed onto C18 bonded phase and eluted with methanol. The recovered residue is essentially free of sample matrix chrom...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(81)91245-5
更新日期：1981-08-01 00:00:00
abstract：OBJECTIVE:Neonatal abstinence syndrome (NAS) is a rising concern with unknown long-term effects. It is apparent that higher cost of care, impact on the community and reduced quality of life are associated with similar etiologies (e.g., fetal alcohol syndrome). Detection of drug exposure in utero allows for earlier inte...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2017.09.016
更新日期：2017-12-01 00:00:00
abstract：:Bone metabolism is assessed using biochemical bone turnover markers (BTM). BTM reflect the metabolic effect of drugs on bone turnover, help to establish the lowest dose inducing the largest change in the BTM, predict treatment-related reduction in fracture risk, and are helpful in bridging studies. Changes in BTM duri...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2012.01.022
更新日期：2012-08-01 00:00:00
abstract：OBJECTIVES:The concentration of tyrosine and the ratio of branch-amino acid to the aromatic amino acid in phenylketonuria (PKU) patients are much lower than that of normal people, which reveal that PKU patients have amino acid metabolism disorder. The aim of the present study was to investigate the arginine level in bl...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2013.10.028
更新日期：2013-12-01 00:00:00
abstract：OBJECTIVE:Quantitative sweat production and -ionic composition in Essential Hyperhidrosis (EH), and the effects of T2-T3 thoracoscopic sympathicolysis (TS) hereon, are unknown. Standardised pilocarpine iontophoresis sweat tests were performed before and after TS in order to study these issues. DESIGN AND METHODS:Piloc...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(96)00165-8
更新日期：1997-03-01 00:00:00
abstract：OBJECTIVES:To examine whether the proportions of blood leukocyte subsets are altered in COPD, and to assess the disturbances in signalling pathways in the leukocytes of COPD patients, with respect to smoking history. DESIGN AND METHODS:The study was performed at the Faculty of Pharmacy and Biochemistry and University ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2007.12.013
更新日期：2008-04-01 00:00:00
abstract：:A high pressure liquid chromatographic method for the determination of hydrochlorothiazide in serum has been elaborated and verified in a blood level study in normal individuals given a single oral dose of 50 mg hydrochlorothiazide. Based on 5 ml of serum, the limit of detection of the method is 2 ng/ml. ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(78)90434-4
更新日期：1978-08-01 00:00:00
abstract：:In order to provide quality control serum in the hypoglycemic range, pooled human serum was treated with yeast. Yeast destroyed about 50% of the serum glucose in about 4 1/2 hrs. The yeast-treated serum remained suitable for quality control of the most commonly analyzed clinical chemistry constituents which showed onl...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(80)90562-7
更新日期：1980-02-01 00:00:00
