Abstract:
:PTH and PTH-related peptide (PTHrP) have been shown to bind to and activate the same PTH/PTHrP receptor. Recent studies have demonstrated, however, the presence of additional receptors specific for each ligand. We used the PTHrP and PTH/PTHrP receptor gene knock-out models to investigate whether this receptor mediates the actions of both ligands in bone. The similar phenotype of the PTHrP (-/-) and PTH/PTHrP receptor (-/-) animals in the growth plate of the tibia suggests that this receptor mediates the actions of PTHrP. Electron microscopic studies have confirmed the accelerated differentiation and disordered organization of chondrocytes, with the accumulation of large amounts of dispersed glycogen granules in the cytoplasm of proliferative and maturing cells of both genotypes. The contrasting growth plate mineralization patterns of the PTHrP (-/-) and PTH/PTHrP receptor (-/-) mice, however, suggest that the actions of PTHrP and the PTH/PTHrP receptor are not identical. Studies using calvariae from PTH/PTHrP receptor (-/-) embryos demonstrate that this receptor solely mediates the ability of PTH and PTHrP to stimulate adenylate cyclase in bone and to stimulate bone resorption. Furthermore, we show that osteoblasts of PTH/PTHrP receptor (-/-) animals, but not PTHrP (-/-) animals, have decreased levels of collagenase 3, osteopontin, and osteocalcin messenger RNAs. The PTH/PTHrP receptor, therefore, mediates distinct physiologic actions of both PTH and PTHrP.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Lanske B,Divieti P,Kovacs CS,Pirro A,Landis WJ,Krane SM,Bringhurst FR,Kronenberg HMdoi
10.1210/endo.139.12.6361subject
Has Abstractpub_date
1998-12-01 00:00:00pages
5194-204issue
12eissn
0013-7227issn
1945-7170journal_volume
139pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Male Siberian hamsters undergo physiological weight change in changing photoperiod. Weight loss was induced by food restriction in long days to mimic short-day weight loss, or by food restriction superimposed on short-day weight loss, to test the hypothesis that the hypothalamus differentiates between weight change in...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.10.8454
更新日期:2001-10-01 00:00:00
abstract::A chemiluminescent dot blot assay has been developed by our laboratory for rapid determinations of IGF-I in serum-free conditioned media (CM) collected from cultured cells. In contrast to IGF-I radioimmunoassays (RIAs), the IGF binding proteins (IGFBPs) did not interfere with the dot blot assay and did not require the...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.5.5237
更新日期:1997-05-01 00:00:00
abstract::Glucocorticoid (Gc) excess, from endogenous overproduction in disorders of the hypothalamic-pituitary-adrenal axis or exogenous medical therapy, is recognized to cause adverse metabolic side effects. The Gc receptor (GR) is widely expressed throughout the body, including brain regions such as the hypothalamus. However...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1571
更新日期:2016-11-01 00:00:00
abstract::We investigated the effects of intraventricular (ivt) injection of bombesin on the TRH-induced increase in temperature in the midbrain reticular formation (mrf) of the rat brain under pentobarbital anesthesia. Ivt injection of TRH in a dose of 3 nmol significantly antagonized the decrease in brain temperature induced ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-4-1864
更新日期:1986-10-01 00:00:00
abstract::Obesity is often associated with an impairment of the hypothalamic-pituitary-gonadal axis. The leptin-deficient ob/ob mouse model is characterized by a morbid obesity with a sterility in males and females that is corrected by continuous leptin treatment. Since ob/ob mice are maintained on the C57BL/6J inbred genetic b...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.2.6470
更新日期:1999-02-01 00:00:00
abstract::A plasma fraction rich in thyroid hormone-binding globulin (hTBG, human thyropexin) was injected iv into rabbits in order to see whether thyroid hormone concentrations in plasma would increase by return of T3 and T4 from the extravascular space. For this purpose, both [125I]T3 and [131I]T4 were simultaneously injected...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-124-3-1428
更新日期:1989-03-01 00:00:00
abstract::Levels of plasma prolactin were estimated in ovariectomized, estrogen-treated rats following the systemic administration of several neural blocking and stimulating drugs. Phenoxybenzamine, an alpha-adrenergic blocker, at high doses, increased plasma prolactin. Phenotlamine, another alpha-adrenergic blocker, and propra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-2-313
更新日期:1975-02-01 00:00:00
abstract::We have previously demonstrated in vitro that 1alpha,25-dihydroxyvitamin D3 (calcitriol) treatment increases p27 expression and decreases cell proliferation in cultured thyroid carcinoma cell lines. We hypothesized that in vivo treatment with calcitriol would have a beneficial effect on thyroid carcinoma growth and pr...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-0785
更新日期:2004-12-01 00:00:00
abstract::Insulin is a potent regulator of Sp1 transcription factor. To examine if glucagon, which usually antagonizes insulin, regulates Sp1, we assessed the levels of Sp1 by Western blotting from H-411E cells exposed to glucagon with or without insulin. Glucagon alone (1.5 x 10(-9) to 1.5 x 10(-5) M) stimulated Sp1 accumulati...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.143.4.8756
更新日期:2002-04-01 00:00:00
abstract::The inhibitory effect of T3 on TSH release was studied on a population of thyrotroph-enriched cells prepared from bovine pituitary glands by centrifugal elutriation. The cells (2.0 X 10(5)/ml) were cultured for 2 days and then exposed to TRH, phorbol-12 myristate-13 acetate (PMA), or calcium ionophore (A23187) with or...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-122-5-2121
更新日期:1988-05-01 00:00:00
abstract::The pharmacokinetics of TRH and its metabolite deamido-TRH (TRH-OH) were evaluated and compared. After a 60- to 90-min infusion of the appropriate peptide into rats, the MCR, half-life of disappearance (t 1/2), and volume of distribution were calculated. The MCR of TRH averaged 4 ml/min, whereas that of TRH-OH was 6.5...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-3-1031
更新日期:1984-09-01 00:00:00
abstract::The effects of increasing concentrations of leucine (0.2, 2.0, and 15.0 mmol/liter) on glucagon secretion from the perfused rat pancreas were examined at various glucose levels (0, 3.3, or 8.3 mmol/liter) and in the absence or presence of either arginine (5.0 mmol/liter) or glutamine (10.0 mmol/liter). At a low glucos...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-3-1168
更新日期:1985-03-01 00:00:00
abstract::Stimulation of rat granulosa cell aromatase activity by FSH has recently been used as a sensitive biological end point to develop an in vitro FSH bioassay. The present report provides a detailed validation and application of this assay. In the presence of androstenedione and diethylstilbestrol, FSH stimulated estrogen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-4-1570
更新日期:1986-10-01 00:00:00
abstract::We have compared the physicochemical properties of rat uterine nuclear estrogen receptors (ER) labeled with (E2) or the high affinity antiestrogen 4-hydroxytamoxifen [OH Tam; 1-[4-(2-dimethylaminoethoxy)phenyl]-1-(4-hydroxyphenyl) 2-phenyl-but-1-(Z)ene]. Nuclear ER labeled in vivo with either ligand had sedimentation ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-119-2-904
更新日期:1986-08-01 00:00:00
abstract::cAMP is involved in the regulation of secretory activity in lactotrope, thyrotrope, and gonadotrope cells. The present study examined whether pulsatile or intermittent changes in cAMP are more effective than a continuous stimulation in increasing pituitary hormone gene expression. Pituitaries from adult female rats we...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.131.6.1280210
更新日期:1992-12-01 00:00:00
abstract::Apoptosis is a physiological process by which multicellular organisms eliminate unwanted cells. Death factors such as Fas ligand induce apoptosis by triggering a series of intracellular protein-protein interactions mediated by defined motifs found in the signaling molecules. One of these motifs is the death effector d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.139.12.6335
更新日期:1998-12-01 00:00:00
abstract::In different types of mammalian cells, insulin has been shown to promote the release of an inositol phosphate glycan (InsP-glycan) through the hydrolysis of a glycosyl-phosphatidylinositol (glycosyl-PtdIns). This InsP-glycan, which has been demonstrated to be taken up by intact cells, may mediate some of the biologica...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.134.3.8119190
更新日期:1994-03-01 00:00:00
abstract::In GH4C1 cells, membrane depolarization induces a rapid and sustained increase in the cytosolic free calcium concentration ([Ca2+]i). In the present study we have investigated the role of [Ca2+]i in the regulation of basal intracellular pH (pHi). Depolarizing GH4C1 cells in buffer containing 0.4 mM extracellular Ca2+ ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.2.1733720
更新日期:1992-02-01 00:00:00
abstract::IGF-II has been reported to decrease neonatal islet cell apoptosis and in vitro adult islet cell necrosis and apoptosis, but the usefulness of IGF-II in a transplantation setting is unknown. We evaluated the effect of in vitro IGF-II incubations on microencapsulated rat islet survival both in vitro and in minimal mass...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-0185
更新日期:2003-07-01 00:00:00
abstract::The present studies were performed to determine whether the major biologically active metabolite of vitamin D3, 1,25-dihydroxycholecalciferol [1,25(OH)2D3], could influence the activities of rat colonic particulate guanylate cyclase and adenylate cyclase. To address these issues, colonocytes were harvested from Spragu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.5.8104780
更新日期:1993-11-01 00:00:00
abstract::The development of acquired resistance to antihormonal agents in breast cancer is a major therapeutic problem. We have developed a tamoxifen-resistant (TAM-R) MCF-7 breast cancer cell line to investigate the mechanisms behind this condition. Both epidermal growth factor receptor (EGFR) and c-erbB2 mRNA and protein exp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2002-220620
更新日期:2003-03-01 00:00:00
abstract::We have demonstrated TSH binding sites in membranes prepared from guinea pig testis. The binding of TSH to testicular homogenate was localized to the interstitial tissue and showed similar characteristics to the binding of TSH to thyroid membranes with high affinity (Ka = 2.5 X 10(10) M-1) but limited capacity (approx...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-1-6
更新日期:1978-07-01 00:00:00
abstract::RNA blot hybridization analysis with cloned rat CRF precursor (prepro-CRF) cDNA as a probe showed that prepro-CRF mRNA existed in rat hypothalamic and extrahypothalamic brain tissue, whereas it was undetectable in the pituitary and adrenal. To study the effect of glucocorticoid on the level of prepro-CRF mRNA in the h...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-4-1314
更新日期:1985-10-01 00:00:00
abstract::Interferon-tau (IFNtau) is the embryonic signal responsible for pregnancy recognition in ruminants. The primary action of IFNtau is believed to be mediated through inhibition of prostaglandin F(2alpha) (PGF(2alpha)) released from the endometrial epithelial cells in response to oxytocin (OT). Our working hypothesis was...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0704
更新日期:2009-02-01 00:00:00
abstract::This study was undertaken to examine short photoperiod (SD; 8 h of light, 16 h of darkness)-induced alterations in reproductive endocrine and neuroendocrine parameters in the male white-footed mouse, Peromyscus leucopus. Exposure to SD for 8 weeks caused dramatic reductions in testis and seminal vesicle weights, decre...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-2-1119
更新日期:1988-08-01 00:00:00
abstract::Several observations suggest that GH stimulates sebaceous gland growth and development. Therefore, we studied the effects of GH and insulin-like growth factors (IGFs), alone and with androgen, on sebaceous epithelial cell (sebocyte) growth and differentiation in vitro. The rat preputial cell culture model system was u...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.140.9.6957
更新日期:1999-09-01 00:00:00
abstract::Nicotine is a potent stimulus for the secretion of ACTH, and norepinephrinergic neurons originating in the brainstem are involved. Prior reports using in vivo microdialysis in alert rats have shown that nicotine, administered i.p. or into the fourth ventricle, stimulated the release of norepinephrine (NE) into the hyp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.5.5122
更新日期:1997-05-01 00:00:00
abstract::The paucity of clinical and preclinical studies investigating sex differences in sleep has resulted in mixed findings as to the exact nature of these differences. Although gonadal steroids are known to modulate sleep in females, less is known about males. Moreover, little evidence exists concerning the origin of these...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1624
更新日期:2014-01-01 00:00:00
abstract::Activins stimulate FSH synthesis and secretion by pituitary gonadotrope cells. Activin A induction of porcine and murine FSHβ (Fshb) gene transcription in immortalized gonadotropes is dependent on homolog of Drosophila mothers against decapentaplegic (SMAD) proteins as well as the forkhead transcription factor L2 (FOX...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0605
更新日期:2010-11-01 00:00:00
abstract::The sympathetic nervous system is a physiological regulator of bone homeostasis. Autonomic nerves are indeed present in bone, bone cells express the β2-adrenergic receptors (β2AR), and pharmacological or genetic disruption of sympathetic outflow to bone induces bone gain in rodents. These recent findings implied that ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2010-0881
更新日期:2011-04-01 00:00:00