Abstract:
:Several observations suggest that GH stimulates sebaceous gland growth and development. Therefore, we studied the effects of GH and insulin-like growth factors (IGFs), alone and with androgen, on sebaceous epithelial cell (sebocyte) growth and differentiation in vitro. The rat preputial cell culture model system was used to judge differentiation (induction of lipid-forming colonies, LFCs) and DNA synthesis. GH increased sebocyte differentiation. At a dose of 10(-8) M in the presence of micromolar insulin, GH was 3.8 times more potent than IGF-I (38.1+/-4.2%, SEM, vs. 10+/-1.5% LFCs) and 6 times more potent than IGF-II (6+/-0.5% LFCs). IGF-I 10(-8) M alone stimulated a similar amount of differentiation as insulin 10(-6) M, although it was less effective than insulin in augmenting the effect of GH on differentiation. GH had no effect on sebocyte uptake of 3H-thymidine at doses up to 10(-6) M. On the other hand, IGF-I was the most potent stimulus of DNA synthesis (168% of control; P < 0.001 vs. all others). IGF-II 10(-8) M stimulated 3H-thymidine incorporation similarly to insulin 10(-6) M. In the presence of insulin, dihydrotestosterone (DHT) 10(-6) M induced 31.4+/-1.7% LFCs, and there was a tendency of DHT and GH to interact in promoting differentiation. When insulin was omitted from the system, differentiation was decreased overall, but GH +/- DHT slightly improved differentiation. The IGFs had no effect on the response to DHT. DHT decreased DNA synthesis by 40%, an effect unaltered by GH or IGFs. These results suggest that GH and IGFs have different functions in sebaceous cell growth and differentiation: GH stimulated differentiation beyond that found with IGFs or insulin, yet had no effect on DNA synthesis, a parameter stimulated most potently by IGF-I. While GH augmented the effect of DHT on differentiation, the IGFs had no effect on the response of DHT. These data indicate that GH may in part act directly on sebocytes rather than indirectly through IGF production. These data are consistent with the concept that increases in GH and IGF production contribute in complementary ways to the increase in sebum production during puberty and in acromegaly.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Deplewski D,Rosenfield RLdoi
10.1210/endo.140.9.6957subject
Has Abstractpub_date
1999-09-01 00:00:00pages
4089-94issue
9eissn
0013-7227issn
1945-7170journal_volume
140pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::Trans-10, cis-12 conjugated linoleic acid (10,12 CLA) is a dietary fatty acid that promotes weight loss and disproportionate fat loss. Obese mice fed a high-fat, high-sucrose (HFHS) diet containing 10,12 CLA are resistant to weight gain and contain markedly reduced subcutaneous fat and adiponectin, with a concurrent l...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2017-00213
更新日期:2017-09-01 00:00:00
abstract::The formation of a new blood supply, angiogenesis, is an essential component of carcinogenesis and unrestricted tumor growth. A substance capable of inhibiting angiogenesis would be of considerable therapeutic potential in the treatment of cancer. We previously reported that the 16-kilodalton N-terminal fragment of ra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.3.7689950
更新日期:1993-09-01 00:00:00
abstract::Osteoclasts are bone-resorbing cells whose normal function depends in part upon their ability to migrate over the bone surface to initiate new sites of bone resorption. The growth factor/cytokine, colony-stimulating factor-1 (CSF-1), potently stimulates osteoclast motility, in a c-src-dependent fashion. The intracellu...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.6.7480
更新日期:2000-06-01 00:00:00
abstract::Neuropeptide Y (NPY) readily stimulates the release of hypothalamic LHRH and pituitary LH release in intact and gonadal steroid-primed gonadectomized rats. We have now tested the hypothesis that the release and synthesis of hypothalamic NPY may be regulated by gonadal steroids. To measure the effects of gonadal hormon...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.130.6.1375900
更新日期:1992-06-01 00:00:00
abstract::Experiments were performed in vitro to examine the possible role of calcium and calmodulin in GRF-induced somatostatin (SRIF) release from the median eminence. Adult male rats were used as tissue donors. The median eminences were first prestimulated in 0.4 ml Krebs Ringer bicarbonate glucose buffer (pH 7.4) containing...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-123-1-305
更新日期:1988-07-01 00:00:00
abstract::As a model of the changes in peripheral iodothyronine metabolism that occur during ontogeny, we have studied the metabolism of 125I-labeled T4 and several of its partially deiodinated derivatives by the liver of the chick embryo. Homogenates of livers obtained from chicks varying in embryonic age from 8 days to the ti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-107-6-1751
更新日期:1980-12-01 00:00:00
abstract::An estrogen-responsive uterine proenzyme of a proteinase in the immature rat uterus has been known for some time. Its mol wt is 77,000, its N-terminal amino acid sequence is the same as prothrombin's for 15 residues, it contains gamma-carboxyl glutamate residues, its biosynthesis is prevented by warfarin, it cross-rea...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-1-167
更新日期:1990-01-01 00:00:00
abstract::Androgenic glands (AGs) of the freshwater prawn Macrobrachium rosenbergii were subjected to endocrine manipulation, causing them to hypertrophy. Transcripts from these glands were used in the construction of an AG cDNA subtractive library. Screening of the library revealed an AG-specific gene, termed the M. rosenbergi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0906
更新日期:2009-03-01 00:00:00
abstract::We have recently identified, characterized, and cloned a luteal microsomal 32-kDa phosphoprotein that we named PRAP (for PRL-receptor associated protein), and we have demonstrated that PRAP binds to the intracellular domain of the short but not the long form of the PRL receptor. In this study, we used PRAP cDNA to exa...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.8.5336
更新日期:1997-08-01 00:00:00
abstract::Early life exposure to endocrine-disrupting chemicals (EDCs) is an emerging risk factor for the development of obesity and diabetes later in life. We previously showed that prenatal exposure to the EDC tributyltin (TBT) results in increased adiposity in the offspring. These effects linger into adulthood and are propag...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2018-00056
更新日期:2018-08-01 00:00:00
abstract::Although the interactions between sex steroids and GnRH have been extensively studied, little is known about the mechanism of estradiol (E2) effects on GnRH secretion. In the present study, we used retrochiasmatic hypothalamic explants of 50-d-old male rats, and we observed that E2 significantly increased the glutamat...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2004-1265
更新日期:2005-05-01 00:00:00
abstract::Loss-of-function mutations in the X-linked immunoglobulin superfamily, member 1 (IGSF1) gene cause central hypothyroidism. IGSF1 is a transmembrane glycoprotein of unknown function expressed in thyrotropin (TSH)-producing thyrotrope cells of the anterior pituitary gland. The protein is cotranslationally cleaved, with ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2016-1788
更新日期:2017-04-01 00:00:00
abstract::The influences of mating on serum luteinizing hormone (LH) and follicle stimulating hormone (FSH) levels were evaluated in 4-day cyclic proestrous rats of the Wistar strain. Blood samples were collected via guillotine from mated and unmated controls at 1900-2000 h or 2100--2200 h. Serum LH levels were determined by tw...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-100-2-394
更新日期:1977-02-01 00:00:00
abstract::Steady state levels of plasma membrane receptors (measured in radioligand assays) result from processes that contribute to the production of receptors (synthesis, recycling, and unmasking) as well as those that contribute to the loss of receptors (degradation, internalization, and inactivation). Accordingly, we have p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-5-2577
更新日期:1990-05-01 00:00:00
abstract::Glucocorticoid feedback inhibition at the level of the brain is extremely complex, involving feedback at both hypothalamic and suprahypothalamic levels. The hippocampus has been implicated as a suprahypothalamic mediator of such feedback, based on numerous lesion, stimulation, and steroid implantation studies. These r...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-6-2881
更新日期:1989-12-01 00:00:00
abstract::Administration of ovine or rat PRL to animals, including man, has resulted in decreased urine volume and increased urine osmolality. Contamination of PRL preparations with vasopressin is the most likely explanation for the apparent antidiuretic effect. In this study, diabetes insipidus rats lacking vasopressin(homozyg...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-105-4-1001
更新日期:1979-10-01 00:00:00
abstract::11 beta-Hydroxysteroid dehydrogenase (11 beta HSI) is an enzyme complex responsible for the conversion of hormonally active cortisol to inactive cortisone; two isoforms of the enzyme have been cloned and characterized. Clinical observations from patients with the hypertensive syndrome apparent mineralocorticoid excess...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.3.4994
更新日期:1997-03-01 00:00:00
abstract::The initial steps involved in mediating the transduction of PTH signal via its G protein-coupled receptors are well understood and occur through the activation of cAMP and phospholipase C pathways. However, the cellular and molecular mechanisms for subsequent receptor desensitization are less well understood. Recently...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.141.1.7229
更新日期:2000-01-01 00:00:00
abstract::There is a growing body of evidence that subtle decreases in maternal thyroid hormone during gestation can impact fetal brain development. The present study examined the impact of graded levels of thyroid hormone insufficiency on brain development in rodents. Maternal thyroid hormone insufficiency was induced by expos...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1276
更新日期:2007-06-01 00:00:00
abstract::A proteinaceous secretion from phagocytizing polymorphonuclear leukocytes, termed "leukocytic endogenous mediator" (LEM), has been shown to have marked effects on hepatic amino acid transport and RNA and protein synthesis. A single injection of LEM results in a marked accumulation of labeled nonmetabolizable model ami...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-3-651
更新日期:1975-03-01 00:00:00
abstract::A complementary DNA, uterine-ovarian-specific gene 44 (UO-44), has been isolated from tamoxifen-induced rat uterine complementary DNA library using differential display techniques. UO-44 transcripts are found to be abundant in the uterus and ovary. UO-44 gene expression in the uterus is strictly regulated by estrogens...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.7.8247
更新日期:2001-07-01 00:00:00
abstract::The structural requirements for the inhibition of net bone collagen synthesis by parathyroid hormone (PTH) in vitro have been examined by study of the effects of selected fragments and analogs of bovine PTH (bPTH) upon the incorporation of [3H]proline into collagenase-digestible and -nondigestible proteins by neonatal...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-108-1-103
更新日期:1981-01-01 00:00:00
abstract::GH is considered to play a role in the pathogenesis of diabetic retinopathy, causing neovascularization in the retina. The present study was conducted to assess the possibility that GH may play a role in ocular development by determining whether GH is expressed in the eye of the chicken during development. In the 17-d...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.142.12.8552
更新日期:2001-12-01 00:00:00
abstract::The specific uptake by rat liver of human (hGH) and bovine (bGH) GHs labeled with 125I was studied by an in vivo procedure. A significant reduction of the uptake was observed when labeled hormones were injected together with different amounts of the corresponding native GH. This reduction was dose dependent, and the c...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-103-4-1190
更新日期:1978-10-01 00:00:00
abstract::Aside from studies of a possible synergism between T3 and glucocorticoids in regulating GH mRNA, there have been few previous studies of the modulation by hormones and other factors of glucocorticoid hormone regulation of pituitary cell GH gene expression. We have employed a serum-free medium containing no exogenously...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-5-1986
更新日期:1987-05-01 00:00:00
abstract::A rat medullary thyroid carcinoma, which was previously shown to produce high levels of immunoreactive cholecystokinin (CCK), was used to establish a stable cell line. Transplantable tumors were subjected to four series of alternate in vitro and in vivo passages. Cells were prepared from the fourth series of tumors un...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-2-850
更新日期:1989-08-01 00:00:00
abstract::Histamine has been shown to be involved in atherosclerosis and coronary heart disease. Little information is available regarding the effects of histamine on lipoprotein metabolism. In the current study, we investigated the effects of histamine on the expression of hepatic low density lipoprotein (LDL) receptors and on...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.5.5115
更新日期:1997-05-01 00:00:00
abstract::A side population (SP) has been identified in a number of tissues, where it typically represents a small population enriched in stem/progenitor cells. In this study we show that the adult mouse anterior pituitary (AP) also contains a characteristic SP displaying verapamil-sensitive Hoechst dye efflux capacity. A major...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2005-0185
更新日期:2005-09-01 00:00:00
abstract::Leptin, a pleiotropic adipokine, is a central regulator of appetite and weight and a key immunomodulatory protein. Although inborn leptin deficiency causes weight gain, it is unclear whether induced leptin deficiency in adult wild-type animals would be orexigenic. Previous work with a potent competitive leptin antagon...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-1706
更新日期:2009-07-01 00:00:00
abstract::Specialized metabolic sensors in the hypothalamus regulate blood glucose levels by influencing hepatic glucose output and hypoglycemic counterregulatory responses. Hypothalamic reactive oxygen species (ROS) may act as a metabolic signal-mediating responses to changes in glucose, other substrates and hormones. The role...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-2054
更新日期:2016-12-01 00:00:00