当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Intermolecular interaction of lens crystallins: from rotationally mobile to immobile states at high protein concentrations.

Intermolecular interaction of lens crystallins: from rotationally mobile to immobile states at high protein concentrations.

Abstract：

:The conformation of lens crystallins in vivo or in a highly concentrated solution is not well established. Most studies were carried out in dilute solutions in which protein-protein interaction is minimal. In order to see whether there is conformational change (tertiary and secondary structures) when crystallin solutions are brought to high concentrations, we have performed the following molecular spectroscopic measurements: circular dichroism (CD) and Fourier transform infrared (FTIR). Near-UV CD measurements showed a more than two-fold increase in CD intensity (molar ellipticity) for the total water-soluble (WS) protein from young calf lens nucleus in a highly concentrated solution (> 300 mg/ml in a 0.01-mm cell), when compared with a dilute solution (1000-fold dilution in a 10-mm cell). The individual crystallins in concentrated solutions also showed an increase in CD intensity, but of different magnitude: alpha-crystallin > beta-crystallin > gamma-crystallin. The increased CD indicates that lens crystallins are in a more compact structure in highly concentrated solutions; they likely undergo a transition from a mobile to an immobile state. Change in near-UV CD usually is caused by restricted mobility of aromatic side groups, particularly Trp. The transition involves not only a change in protein tertiary and/or quaternary structure, but also in protein backbone structure. The change of protein backbone structure was drawn from FTIR measurements. FTIR spectra, sensitive to the secondary structure in the amide I region, could be measured for a highly concentrated solution for which far-UV CD measurement is not feasible. The secondary structure that showed prominent change for alpha-crystallin in a highly concentrated solution was beta-conformation: increase in beta-turn with a concomitant decrease of alpha-helix structure.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Liang JJ,Chakrabarti B

doi

10.1006/bbrc.1998.8640

subject

Has Abstract

pub_date

1998-05-19 00:00:00

pages

441-5

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(98)98640-5

journal_volume

246

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Fission yeast LAMMER kinase Lkh1 regulates the cell cycle by phosphorylating the CDK-inhibitor Rum1.

    abstract：:In eukaryotes, LAMMER kinases are involved in various cellular events, including the cell cycle. However, no attempt has been made to investigate the mechanisms that underlie the involvement of LAMMER kinase. In this study, we performed a functional analysis of LAMMER kinase using the fission yeast, Schizosaccharomyce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.01.082

    authors： Yu EY,Lee JH,Kang WH,Park YH,Kim L,Park HM

    更新日期：2013-03-01 00:00:00

  • Lipopolysaccharide prevents apoptosis induced by brefeldin A, an endoplasmic reticulum stress agent, in RAW 264.7 cells.

    abstract：:The effect of lipopolysaccharide (LPS) on the cell death induced by endoplasmic reticulum (ER) stress agents in RAW 264.7 cells was studied. LPS prevented the cell death by brefeldin A, but not thapsigargin and tunicamycin. CpG DNA as well as LPS prevented brefeldin A-induced cell death whereas tumor necrosis factor-a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.12.050

    authors： Islam S,Hassan F,Tumurkhuu G,Ito H,Koide N,Mori I,Yoshida T,Yokochi T

    更新日期：2006-02-10 00:00:00

  • High-active truncated luciferase of copepod Metridia longa.

    abstract：:The technology of real-time imaging in living cells is crucial for understanding of intracellular events. For this purpose, bioluminescent reporters have been introduced as sensitive and convenient tools. Metridia luciferase (MLuc) from the copepod Metridia longa is a coelenterazine-dependent luciferase containing a n...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.11.063

    authors： Markova SV,Burakova LP,Vysotski ES

    更新日期：2012-01-06 00:00:00

  • Simultaneous dual-excitation ratiometry using orthogonal linear polarized lights.

    abstract：:Dual-excitation ratiometric dyes permit quantitative Ca2+ measurements by minimizing the effects of several artifacts that are unrelated to changes in the concentration of free Ca2+ ([Ca2+]). These dyes are excited alternately at two different wavelengths, and the pair of intensity measurements must be collected seque...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.03.009

    authors： Fukano T,Shimozono S,Miyawaki A

    更新日期：2004-04-23 00:00:00

  • Oleanolic acid prevents cartilage degeneration in diabetic mice via PPARγ associated mitochondrial stabilization.

    abstract：:Hyperglycemia-induced cartilage degeneration induces osteoarthritis (OA). Since oleanolic acid (OLA) have several pharmacological effects such as anti-inflammatory, anti-oxidant, we hypothesized it possesses protection against high glucose injured cartilage. We now report that OLA decreased type X collagen and reverse...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.06.127

    authors： Kang X,Yang Z,Sheng J,Liu JB,Xie QY,Zheng W,Chen K

    更新日期：2017-08-26 00:00:00

  • Repair of psoralen interstrand cross-links in Xenopus laevis egg extracts is highly mutagenic.

    abstract：:The recognition and removal of interstrand cross-links is perhaps the least understood of all repair pathways in eukaryotic cells. We have shown previously that uncoupling of cross-links occurs in mammalian cell extracts and have identified a number of factors that mediate this process. However, we have not observed c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.08.042

    authors： Lu X,Zhang N,Vasquez K,Barton M,Legerski R

    更新日期：2005-10-14 00:00:00

  • Regulation of sterol biosynthesis in sunflower by 24(R,S),25-epiminolanosterol, a novel C-24 methyl transferase inhibitor.

    abstract：:Whereas sitosterol and 24(28)-methylene cycloartanol were competitive inhibitors (with Ki = 26 microM and 14 microM, respectively), 24(R,S)-25-epiminolanosterol was found to be a potent non-competitive inhibitor (Ki = 3.0 nM) of the S-adenosyl-L-methionine-C-24 methyl transferase from sunflower embryos. Because the gr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)92021-b

    authors： Nes WD,Janssen GG,Norton RA,Kalinowska M,Crumley FG,Tal B,Bergenstrahle A,Jonsson L

    更新日期：1991-05-31 00:00:00

  • Downregualtion of dynamin-related protein 1 attenuates glutamate-induced excitotoxicity via regulating mitochondrial function in a calcium dependent manner in HT22 cells.

    abstract：:Glutamate-mediated excitotoxicity is involved in many acute and chronic brain diseases. Dynamin related protein 1 (Drp-1), one of the GTPase family of proteins that regulate mitochondrial fission and fusion balance, is associated with apoptotic cell death in cancer and neurodegenerative diseases. Here we investigated ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.11.072

    authors： Zhang C,Yuan XR,Li HY,Zhao ZJ,Liao YW,Wang XY,Su J,Sang SS,Liu Q

    更新日期：2014-01-03 00:00:00

  • Distribution of 1,25-dihydroxyvitamin D3 receptor and 25-hydroxyvitamin D3-24-hydroxylase mRNA expression along rat nephron segments.

    abstract：:Distribution of mRNA expression of 1,25-dihydroxyvitamin D3 receptor (VDR) and 25-hydroxyvitamin D3-24-hydroxylase (24-OHase) along the microdissected rat nephron segments was examined by reverse transcription-polymerase chain reaction (RT-PCR) technique. VDR mRNA was abundantly expressed in cortical segments (glomeru...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.1872

    authors： Iida K,Taniguchi S,Kurokawa K

    更新日期：1993-07-30 00:00:00

  • Up-regulated expression of beta3 integrin induced by dengue virus serotype 2 infection associated with virus entry into human dermal microvascular endothelial cells.

    abstract：:Permeability alteration of microvascular endothelia is a factor in the plasma leakage produced by dengue virus (DV) infection, and beta3 integrin plays central roles in maintaining capillary integrity and regulating vascular permeability. In this study, interaction between beta3 integrin and DV serotype 2 (DV2) was in...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.03.051

    authors： Zhang JL,Wang JL,Gao N,Chen ZT,Tian YP,An J

    更新日期：2007-05-11 00:00:00

  • Glutathione transferase pi its minimal promoter and downstream cis-acting element.

    abstract：:Fragments of the human glutathione S-transferase pi gene and 15 kb of its 5' flanking region have been fused to the chloramphenicol acetyl transferase (CAT) reporter gene. Transfection into a number of human cell lines (Hela, HepG2, MCF7 and EJ) has demonstrated that the AP1 binding site, located between nucleotides -...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)90914-s

    authors： Xia CL,Cowell IG,Dixon KH,Pemble SE,Ketterer B,Taylor JB

    更新日期：1991-04-15 00:00:00

  • Overexpression of glia maturation factor (GMF) in PC12 pheochromocytoma cells activates p38 MAP kinase, MAPKAP kinase-2, and tyrosine hydroxylase.

    abstract：:In order to study the intracellular regulatory function of glia maturation factor (GMF) in neuronal cells, we achieved a 10-fold overexpression of GMF in the rat pheochromocytoma cell line PC12 by infection with a replication-defective human adenovirus carrying a rat GMF cDNA insert. These cells showed a 3.6-fold incr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9301

    authors： Zaheer A,Lim R

    更新日期：1998-09-18 00:00:00

  • Green tea epigallocatechin-3-gallate inhibits microsomal prostaglandin E(2) synthase-1.

    abstract：:Prostaglandin (PG)E(2) is a critical lipid mediator connecting chronic inflammation to cancer. The anti-carcinogenic epigallocatechin-3-gallate (EGCG) from green tea (Camellia sinensis) suppresses cellular PGE(2) biosynthesis, but the underlying molecular mechanisms are unclear. Here, we investigated the interference ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.08.005

    authors： Koeberle A,Bauer J,Verhoff M,Hoffmann M,Northoff H,Werz O

    更新日期：2009-10-16 00:00:00

  • Replacement of methionine as the axial ligand of Achromobacter cycloclastes cytochrome c554 at high pH values revealed by absorption, EPR and MCD spectroscopy.

    abstract：:Cytochrome c554 from the denitrifying bacterium Achromobacter cycloclastes is a monoheme class II c-type cytochrome with a His-Met axial coordination at neutral pH. The amino acid composition and the N-terminal sequence of the cytochrome have been determined. Subsequent determination of the pH-dependence of the redox ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2434

    authors： Saraiva LM,Thomson AJ,Le Brun NE,Liu MY,Payne WJ,LeGall J,Moura I

    更新日期：1994-10-14 00:00:00

  • Binding of two zinc finger nuclease monomers to two specific sites is required for effective double-strand DNA cleavage.

    abstract：:Custom-designed zinc finger nucleases (ZFNs) are becoming powerful tools in gene targeting-the process of replacing a gene within a genome by homologous recombination. Here, we have studied the DNA cleavage by one such ZFN, DeltaQNK-FN, in order to gain insight into how ZFNs cleave DNA and how two inverted sites promo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.07.021

    authors： Mani M,Smith J,Kandavelou K,Berg JM,Chandrasegaran S

    更新日期：2005-09-09 00:00:00

  • Cloning and characterization of the gene encoding the mouse peptide transporter PEPT2.

    abstract：:Here we describe the cDNA structure, genomic organization, chromosomal localization, and promoter analysis of the mouse peptide transporter PEPT2. The PEPT2-cDNA is 3987 bp long and encodes a protein of 729 amino acids. The functional properties, analyzed by expression in Xenopus laevis oocytes, showed a typical PEPT2...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.3546

    authors： Rubio-Aliaga I,Boll M,Daniel H

    更新日期：2000-09-24 00:00:00

  • S100 to receptor for advanced glycation end-products binding assay: looking for inhibitors.

    abstract：:Secreted by tumor and stromal cells, S100 proteins exert their biological functions via the interaction with surface receptors. The most described receptor is the receptor for advanced glycation end-products (RAGE), thereby participating in the S100-dependent cell migration, invasion, tumor growth, angiogenesis and me...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.02.143

    authors： Padilla L,Dakhel S,Hernández JL

    更新日期：2014-03-28 00:00:00

  • S-Adenosyl-l-methionine activates the cardiac ryanodine receptor.

    abstract：:S-Adenosyl-l-methionine (SAM) is the biological methyl-group donor for the enzymatic methylation of numerous substrates including proteins. SAM has been reported to activate smooth muscle derived ryanodine receptor calcium release channels. Therefore, we examined the effects of SAM on the cardiac isoform of the ryanod...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.03.146

    authors： Kampfer AJ,Balog EM

    更新日期：2008-07-11 00:00:00

  • Regulation of c-myc through intranuclear localization of its RNA subspecies.

    abstract：:We used fluorescence in situ hybridization (FISH) to detect c-myc RNA subspecies in human COLO 320DM tumor cells. Although the FISH procedure removed the majority of RNAs from the nucleolus, c-myc RNA continued to be detected in both the nucleoplasm and nucleolus. This finding suggests stable association between c-myc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.05.201

    authors： Shimizu N,Kawamoto JK,Utani K

    更新日期：2007-08-03 00:00:00

  • A novel in vivo regulatory role of P-glycoprotein in alloimmunity.

    abstract：:P-glycoprotein (P-gp) is required for adaptive immunity through defined functions in T cell activation and antigen presenting cell (APC) maturation. The potential role of P-gp as an in vivo regulator of alloimmunity is currently unknown. Here we show that P-gp blockade prolongs graft survival in a murine heterotopic c...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.03.040

    authors： Izawa A,Schatton T,Frank NY,Ueno T,Yamaura K,Pendse SS,Margaryan A,Grimm M,Gasser M,Waaga-Gasser AM,Sayegh MH,Frank MH

    更新日期：2010-04-09 00:00:00

  • Circular dichroism spectra demonstrate formation of the thrombin-binding DNA aptamer G-quadruplex under stabilizing-cation-deficient conditions.

    abstract：:It is noteworthy that the formation of the DNA G-quadruplex is induced by factors other than stabilizing cations because this event probably occurs in living cells. Previous studies have shown that thrombin-binding DNA aptamer (TBA) forms a chair-type intramolecular G-quadruplex structure that binds with thrombin prot...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.11.088

    authors： Nagatoishi S,Tanaka Y,Tsumoto K

    更新日期：2007-01-19 00:00:00

  • Conserved GC-boxes, E-box and GATA motif are essential for GATA-4 gene expression in P19CL6 cells.

    abstract：:The promoter of the GATA-4 gene was analyzed in P19CL6 cells. A 124bp segment containing conserved two GC-boxes and E-box was essential for the basal promoter activity, as determined with a transient luciferase reporter gene assay. However, an extended 1312 bp reporter construct but not the 124 bp segment, when ligate...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.08.036

    authors： Ishibashi T,Yokura Y,Ohashi K,Yamamoto H,Maeda M

    更新日期：2011-09-23 00:00:00

  • Long-term phorbol ester treatment dissociates phospholipase D activation from phosphoinositide hydrolysis and prostacyclin synthesis in endothelial cells stimulated with bradykinin.

    abstract：:Bovine pulmonary artery endothelial cells (BPAEC) were prelabeled with [3H]choline or [3H]myristic acid to selectively label endogenous phosphatidylcholine. BPAEC were stimulated with ATP and bradykinin (BK), and phospholipase D (PLD) activation was detected as a 4-fold increase in [3H]choline in cells prelabeled with...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)91072-3

    authors： Martin TW,Feldman DR,Goldstein KE,Wagner JR

    更新日期：1989-11-30 00:00:00

  • The establishment of a novel high-throughput screening system using RNA-guided genome editing to identify chemicals that suppress aldosterone synthase expression.

    abstract：:Aldosterone is synthesized in the adrenal by the aldosterone synthase CYP11B2. Although the control of CYP11B2 expression is important to maintain the mineral homeostasis, its overexpression induced by the depolarization-induced calcium (Ca2+) signaling activation has been reported to increase the synthesis of aldoste...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.11.020

    authors： Ito R,Morita M,Nakano T,Sato I,Yokoyama A,Sugawara A

    更新日期：2021-01-01 00:00:00

  • C-type natriuretic peptide is a growth inhibitor of rat vascular smooth muscle cells.

    abstract：:C-type natriuretic peptide (CNP) which potently stimulates particulate guanylate cyclase activity in cultured rat vascular smooth muscle cells (VSMC) inhibited serum-induced DNA synthesis of the cells 10-fold more effectively than alpha-human atrial natriuretic peptide (alpha-hANP). The inhibitory effect of CNP was mi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)90627-j

    authors： Furuya M,Yoshida M,Hayashi Y,Ohnuma N,Minamino N,Kangawa K,Matsuo H

    更新日期：1991-06-28 00:00:00

  • Mast cell chemotactic activity of RANTES.

    abstract：:RANTES is a cytokine produced by activated T-lymphocytes that has been shown to exert chemotactic activity for memory-type CD4 T-lymphocytes and eosinophils. In this study, RANTES caused directional migration of human mast cells. When compared to other potential chemoattractants of the same cells, RANTES was found to ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1505

    authors： Mattoli S,Ackerman V,Vittori E,Marini M

    更新日期：1995-04-06 00:00:00

  • Atorvastatin induces tissue transglutaminase in human endothelial cells.

    abstract：:Tissue transglutaminase (tTgase) contributes to the organisation of the basement membrane and is therefore thought to be important for the integrity and stability of the vessel wall. In the present study, we hypothesised that the HMG-CoA reductase inhibitor atorvastatin may up-regulate the tTgase expression in endothe...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.07.087

    authors： Soehnlein O,Eskafi S,Schmeisser A,Kloos H,Daniel WG,Garlichs CD

    更新日期：2004-09-10 00:00:00

  • Preparation and partial characterization of polyclonal antibodies raised against a preselected sequence of calcium dependent lectins.

    abstract：:The synthetic-peptide strategy was used to generate antibodies raised against calcium-dependent lectins of vertebrates. We demonstrate that a synthetic peptide predicted from the amino acid sequence of the carbohydrate recognition domain can induce blocking antibodies which would react with, or in close vicinity of, t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)91975-x

    authors： Blanck O,Thibault V,Granier C,Van Rietschoten J,Courageot J,Miquelis R

    更新日期：1990-06-29 00:00:00

  • Phosphorylation of the band 4.1-like proteins of the bovine lens.

    abstract：:Crude membrane fractions prepared from the superficial cortical fiber cells of bovine lenses were incubated with [gamma-32P]-ATP. Membrane-associated 4.1-like proteins were then immunoprecipitated and analyzed for 32P-incorporation. Band 4.1-like proteins of 150 kd, 80 kd, and 78 kd were found to be labeled in a time-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90308-0

    authors： Aster JC,Brinn K,Maisel H,Brewer GJ

    更新日期：1985-02-15 00:00:00

  • Hd3a and OsFD1 negatively regulate rice resistance to Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola.

    abstract：:In rice, Hd3a, GF14 and OsFD1 proteins, forming florigen activation complex, are key components in flowering time regulation. GF14 genes in rice response to biotic and abiotic stress has also been well addressed. The role of GF14e in rice defense has been well studied. However, whether Hd3a and OsFD1 play roles in def...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.03.169

    authors： Ke Y,Wu M,Zhang Q,Li X,Xiao J,Wang S

    更新日期：2019-06-11 00:00:00