Abstract:
:Distribution of mRNA expression of 1,25-dihydroxyvitamin D3 receptor (VDR) and 25-hydroxyvitamin D3-24-hydroxylase (24-OHase) along the microdissected rat nephron segments was examined by reverse transcription-polymerase chain reaction (RT-PCR) technique. VDR mRNA was abundantly expressed in cortical segments (glomeruli, proximal convoluted tubule, proximal straight tubule, cortical thick ascending limb, distal convoluted tubule and cortical collecting duct), and it was also detected in outer medullary segments (medullary thick ascending limb, outer medullary collecting duct). Messenger RNA of 24-OHase was abundant in proximal convoluted tubule, and it was also present in proximal straight tubule, but not in other segments. The results demonstrate that in contrast to the expression of 24-OHase mRNA which is limited to the segments where the activity of this enzyme has been demonstrated, the expression of VDR mRNA is ubiquitous along the nephron segments.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Iida K,Taniguchi S,Kurokawa Kdoi
10.1006/bbrc.1993.1872subject
Has Abstractpub_date
1993-07-30 00:00:00pages
659-64issue
2eissn
0006-291Xissn
1090-2104pii
S0006-291X(83)71872-3journal_volume
194pub_type
杂志文章abstract::Retinyl ester concentration is regulated by retinoic acid (RA) through an autoregulatory loop, which acts on lecithin:retinol acyltransferase (LRAT). We tested whether retinol esterification activity is downregulated in human mammary carcinoma cells and whether LRAT expression is RAR-regulated. Normal human mammary ep...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.2000.3995
更新日期:2000-12-29 00:00:00
abstract::The present study was conducted to determine whether fetuin-A, a dominant serum protein plays a role in chemo-attraction and chemo-invasion of carcinoma cells in vitro. Serum is normally used as positive chemotaxis control in Boyden chamber motility assays, prompting the need to identify the factor/s in serum that con...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2013.07.125
更新日期:2013-09-06 00:00:00
abstract::The coding sequence of type II phospholipase A2 from human placenta was cloned in a bovine papilloma virus-derived eukaryotic expression vector under the control of the metallothionein promoter. Stably transfected C127 mouse fibroblast lines were obtained with this vector. These transfected cells overexpressed a funct...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(91)91035-b
更新日期:1991-08-15 00:00:00
abstract::The 3.6 kb HindIII DNA fragment of B. sphaericus 1593M chromosomal DNA bears two genes encoding two polypeptides of 41.9 kDa (protein "42") and 51.4 kDa (protein "51"). DNA fragments carrying only one of these two genes when expressed in E. coli yield products that are inactive towards Culex larvae. The larvicidal act...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(89)91828-7
更新日期:1989-11-15 00:00:00
abstract::The use of combined hormone replacement therapy (HRT) with oestrogens and progestins in postmenopausal women has been associated with an increased risk for developing breast cancer. The reasons are not fully understood, but influence of HRT on endogenous conversion of female sex hormones may be involved. The expressio...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2012.04.116
更新日期:2012-05-25 00:00:00
abstract::The prevalence of nonalcoholic fatty liver disease (NAFLD) is high and increasing throughout the world. The intense sterile inflammation stemming from steatosis plays a significant role in the development of NAFLD, but the mechanism is unclear. We found that the NLRC4 inflammasome was activated in an in vitro cell mod...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2019.02.099
更新日期:2019-04-09 00:00:00
abstract::The cellular response to DNA damage induced by gamma-irradiation activates cell-cycle arrest to permit DNA repair and to prevent replication. Cyclin D1 is the key molecule for transition between the G1 and S phases of the cell-cycle, and amplification or overexpression of cyclin D1 plays pivotal roles in the developme...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2008.11.132
更新日期:2009-01-23 00:00:00
abstract::Inhibition of the infectivity and cytopathic effect of human immunodeficiency virus type 1 (HIV-1) by the immunoactive fractions obtained from LEM, which is an extract of the culture medium of Lentinus edodes mycelia, is reported. A purified fraction, EPS4, obtained from LEM by ethanol precipitation followed by hydrop...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(89)91665-3
更新日期:1989-04-14 00:00:00
abstract::Gelsolin, a Ca(2+)-dependent actin regulatory protein, was recently suggested to participate in apoptosis regulation. In this study, we found that the level of gelsolin is elevated in senescent human diploid fibroblasts (HDFs) and also in the tissues of old rats, i.e., in the liver, kidney, heart, spleen, stomach, and...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2003.11.061
更新日期:2003-12-26 00:00:00
abstract::Information on the immune response against H5N1 within the lung is lacking. Here we describe the sustained antiviral immune responses, as indicated by the expression of MxA protein and IFN-alpha mRNA, in autopsy lung tissue from an H5N1-infected patient. H5N1 infection of primary bronchial/tracheal epithelial cells an...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2010.07.017
更新日期:2010-08-06 00:00:00
abstract::Explaining why type I collagens are preferentially preserved in the geological time scale remains a challenge. Several pieces of evidence indicate that its rich content in the bone and its unique, stable structure played key roles in its preservation. By considering the distinct thermal stability of amino acids, we re...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章,评审
doi:10.1016/j.bbrc.2012.04.122
更新日期:2012-05-25 00:00:00
abstract::Recent profile studies of microRNA (miRNA) expression have documented a deregulation of miRNA (miR-320a) in human colorectal carcinoma. However, its expression pattern and underlying mechanisms in the development and progression of colorectal carcinoma has not been elucidated clearly. Here, we performed real-time PCR ...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2012.03.075
更新日期:2012-04-20 00:00:00
abstract::Mitochondria dysfunction plays a significant role in the apoptosis of retinal cells. Diabetes activates retinal matrix metalloproteinases (MMP-9 and MMP-2), damages retinal mitochondria and activates the apoptotic machinery. This study is to investigate the temporal relationship between the activation of retinal MMPs ...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2013.07.066
更新日期:2013-09-06 00:00:00
abstract::With age human collagen demonstrates, amongst other changes, reductions in solubility, elasticity and permeability. Many of these changes have been attributed to non-enzymic glycosylation (glycation)-a spontaneous addition of sugar molecules to any protein with free amino groups. The resulting formation and accumulati...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1994.1282
更新日期:1994-03-15 00:00:00
abstract::Quantitative real-time RT-PCR was used to investigate the effects of prototypical drug-metabolizing enzyme inducers rifampicin (Rif), dexamethasone (Dex), and omeprazole (Ome) on mRNA expression levels of the housekeeping genes beta-actin (ACTB), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), beta-glucuronidase (GU...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2006.06.012
更新日期:2006-08-04 00:00:00
abstract::We have purified and characterized recombinant Xenopus bone morphogenetic proteins (xBMPs): homodimers of xBMP-4, 7 and heterodimers (xBMP-4/7) produced by a baculovirus expression system. Highly purified xBMPs had homogeneous NH2-termini predicted from a consensus motif, Arg-X-X-Arg, while they possessed diverse suga...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1995.1712
更新日期:1995-05-25 00:00:00
abstract::Peroxisome proliferator-activated receptor gamma (PPARγ) participates in the process of insulin resistance (IR), a crucial pathophysiology in non-alcoholic fatty liver disease (NAFLD). Meanwhile, suppressor of cytokine signaling3 (SOCS3) also regulates IR in NAFLD. Both PPARγ and SOCS3 play a role in NAFLD through reg...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2018.03.110
更新日期:2018-04-15 00:00:00
abstract::Endothelin-1 (ET-1) and angiotensin II (AII) are potent vasoconstrictor hormones which regulate tissue perfusion and blood pressure. We pharmacologically characterized endothelin and angiotensin receptors mediating contractions of human mammary resistance arteries in myographs for isometric tension recording. ET-1 cau...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1994.2514
更新日期:1994-10-28 00:00:00
abstract::Lon protease has been well studied in many aspects; however, the DNA-binding specificity of Lon in prokaryotes has not been clearly identified. Here we examined the DNA-binding activity of Lon protease alpha-domains from Brevibacillus thermoruber (Bt), Bacillus subtilis (Bs), and Escherichia coli (Ec). MALDI-TOF mass ...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2009.07.118
更新日期:2009-10-09 00:00:00
abstract::The production of 1 alpha,25-dihydroxyvitamin D3 is known to be down regulated by 1 alpha,25-dihydroxyvitamin D3 itself. It was recently shown that liver mitochondrial sterol 27-hydroxylase CYP27, present also in kidney, catalyzes 1 alpha-hydroxylation of 25-hydroxyvitamin D3. Treatment of vitamin D-deficient rats wit...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1995.2443
更新日期:1995-10-04 00:00:00
abstract::Suramin is the first putative growth factor inhibitor in clinical trial that has demonstrated antitumor activity. Bivariate flow cytometric analysis of the effects of suramin on the cell cycle was performed on DU-145 prostate carcinoma cell line. The effects of suramin on the expression of proliferation associated ant...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1994.1741
更新日期:1994-06-15 00:00:00
abstract::CYP27A1 catalyses hydroxylations in the biosynthesis of bile acids and the bioactivation of vitamin D3. We investigated the expression of CYP27A1 in human monocytes, monocyte-derived macrophages, and dendritic cells on mRNA and protein levels as well as its enzymatic activity in comparison with the expression of CYP27...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2006.08.034
更新日期:2006-10-13 00:00:00
abstract::With co-treatment of potassium oxonate (PO) and xanthine sodium salt (XSS), a zebrafish larva model of acute hyperuricemia has been constructed for the first time. The results show PO 200 μM + XSS 10 μM, PO 300 μM + XSS 15 μM, and PO 400 μM + XSS 20 μM can significantly increase the level of uric acid in the zebrafish...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2018.11.050
更新日期:2019-01-08 00:00:00
abstract::Up to 1 mol of phosphoryl groups was incorporated per mol of eukaryotic protein synthesis initiation factor (eIF) 4E following incubation of purified preparations of this factor with purified preparations of a protamine kinase from bovine kidney cytosol. By contrast, purified preparations of two forms of mitogen-activ...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(92)90499-b
更新日期:1992-03-16 00:00:00
abstract::Monoclonal antibody prepared against highly purified rat liver G6PD was used to probe the mode of regulation of this enzyme in a mammalian model system. Material cross-reacting with antibody against liver G6PD was found in similar amounts in extracts of two genetically related rat hepatoma cell lines, only one of whic...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(84)91473-6
更新日期:1984-02-14 00:00:00
abstract:OBJECTIVES AND BACKGROUND:Myocarditis is caused by various agents and autoimmune processes. It is unknown whether viral genome persistence represents inactive remnants of previous infections or whether it is attributed to ongoing adverse processes. The latter also applies to the course of autoimmune myocarditis. One pr...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2005.10.195
更新日期:2006-01-06 00:00:00
abstract::The present study revealed that low density lipoprotein (LDL) obtained from rabbits fed highly purified eicosapentaenoic acid-ethyl ester (EPA-E) (EPA-LDL) was significantly less susceptible to oxidative modification catalyzed by Cu2+ as compared to LDL from rabbits fed a normal diet (control LDL). In a comparison of ...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/s0006-291x(05)81200-8
更新日期:1991-02-28 00:00:00
abstract::A study was undertaken to examine the effects of the tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate, on pancreatic islet B cell differentiated function and number. Islets from 3-day-old rats were maintained in tissue culture for up to 30 days with or without the addition of the phorbol ester. Chan...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/0006-291x(85)91084-8
更新日期:1985-05-16 00:00:00
abstract::The peroxisome proliferator-activated receptor gamma, co-activator 1 alpha(PGC1α) effectively induced the biosynthesis of the mitochondria and the energy metabolism, and also regulated the muscle fiber-type shift. Overexpression of PGC1α gene in mice led to higher oxidative muscle fiber composition in muscle. However,...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1016/j.bbrc.2016.10.113
更新日期:2016-11-25 00:00:00
abstract::Transport of alpha-ketoisocaproate (KIC), a ketoacid originating from leucine and proposed to be involved in the buffering of glutamate in neurones, was studied in neuroblastoma NB-2a cells. The accumulated KIC was mostly transaminated to leucine, while free keto-acid was detectable either only after prolonged times o...
journal_title:Biochemical and biophysical research communications
pub_type: 杂志文章
doi:10.1006/bbrc.1997.7001
更新日期:1997-08-08 00:00:00