Abstract：
:Aging diminishes the amount of phosphotyrosine in the CD3zeta chains of resting and activated mouse CD4 T cells by about threefold and might therefore be expected to a corresponding decline in Zap-70 association with CD3zeta and in Zap-70 kinase function in CD3zeta complexes. We show here that aging leads, unexpectedly, to an approximately twofold increase in the amount of Zap-70 associated with CD3zeta in resting CD4 T cells. There is, however, no effect of age on total intracellular Zap-70 content. Cross-linking CD3 to CD4 leads to an increase of only 50% in the functional activity of Zap-70 in CD3zeta complexes from freshly isolated CD4 T cells of young donors. Compared to Jurkat and HT-2 cells, fresh T cells show both higher baseline levels and lower induced levels of Zap-70 function in CD3zeta complexes. CD4 T cells from old mice have baseline levels of Zap-70 activity similar to those seen in activated T cells from young mice, and these levels do not increase after CD3/CD4 cross-linking. Tyrosine-specific phosphorylation of Zap-70 is also higher at rest in old T cells than in young T cells and inducible only in cells from young donors. These data suggest that age-related defects in T cell activation are not likely to be attributable simply to a decline in Zap-70 association with CD3zeta or to diminished Zap-70 phosphorylation. The increase with age in CD3zeta-Zap association, despite the loss with age in CD3zeta tyrosine phosphorylation, suggests that the pattern of tyrosine phosphate groups among CD3zeta ITAM groups may be different in T cells from young and old donors or that access to ITAM regions within CD3zeta may be blocked by inter- or intramolecular steric hindrance in young CD4 T cells.
journal_name
Cell Immunoljournal_title
Cellular immunologyauthors
Garcia GG,Miller RAdoi
10.1006/cimm.1998.1394subject
Has Abstractpub_date
1998-12-15 00:00:00pages
91-100issue
2eissn
0008-8749issn
1090-2163pii
S0008-8749(98)91394-6journal_volume
190pub_type
杂志文章abstract：:Mice that differ at Igh loci and the reagents that permit resolution of such distinctions are powerful genetic tools for studying B cell heterogeneity. In this report, IgM-allotype-specific ELISA revealed that two of three sources of mice designated as BALB.nude express the Igh-6b (IgMb) allotype characteristic of C57...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1125
更新日期：1993-05-01 00:00:00
abstract：:Recently we have shown that the generation of IFN-gamma-producing cells (IFN-gamma pc) were severely suppressed in cultures of Con A-stimulated splenocytes obtained from HgCl2-exposed BN but not Lewis rats. Since BN rats develop a TH2-biased polyclonal autoimmune syndrome upon exposure to HgCl2, whereas Lewis rats exh...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1027
更新日期：1995-04-01 00:00:00
abstract：:The cellular requirements for the in vitro induction of antigen-specific suppressor T cells were examined. Previous reports indicated that Ia-bearing macrophages and anti-idiotypic B cells are required as accessory cells to facilitate the generation of suppressor effector (TS3) cells which regulate the response to the...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90242-0
更新日期：1988-10-15 00:00:00
abstract：:The effect of prior maternal immunization on the murine offspring response to subsequent immunization with hen egg-white lysozyme was examined. Adult female A/J mice were immunized with 100 micrograms HEL-CFA intraperitoneally 10-27 weeks before conception. The offspring of these experimental female mice were then imm...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1993.1123
更新日期：1993-05-01 00:00:00
abstract：:That M1/70, a monoclonal anti-murine macrophage antibody, recognizes murine natural killer cells (NK) and that there is an increase in NK following intraperitoneal infection with live Listeria monocytogenes (LM) was previously reported. Here, LM-induced NK cells were further characterized with respect to tumor target ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(84)90206-5
更新日期：1984-11-01 00:00:00
abstract：:The biochemical events leading to enhanced membrane expression of HLA-DR and CR3 by human peripheral blood monocytes (MO) following exposure to bacterial lipopolysaccharide (LPS) were examined. In a previous study we demonstrated that an increase in intracellular calcium was necessary, but not sufficient, for MO to in...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90209-7
更新日期：1987-08-01 00:00:00
abstract：:CD20 is expressed strictly by B-cells and is ubiquitously expressed at high surface densities of malignant human B-cells. This suggests that CD20 may be a tumor target for immunotherapy of B-cell lymphomas. Rituximab, a chimeric monoclonal antibody directed against CD20, has been demonstrated to be an effective treatm...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2006.05.005
更新日期：2006-02-01 00:00:00
abstract：:The effects of oral administration of ovalbumin (OVA) on acute OVA-induced arthritis (OIA) in rats, which is mediated by Arthus reaction to the antigen in the joint space, were investigated. The oral administration of OVA before immunization with OVA significantly suppressed the development of acute OIA in a dose-depe...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.1049
更新日期：1997-01-10 00:00:00
abstract：:It has been reported that granulocyte/macrophage colony-stimulating factor (GM-CSF), one of the hemopoietic growth factors which regulates the function of phagocytic cells, is a potent activator of cultured macrophages and induces antimicrobial activities as well as differentiation of precursor cells. In this study, w...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1996.1074
更新日期：1997-02-25 00:00:00
abstract：:Veto cells suppress generation of CD8(+) T cell immune responses in an antigen-specific manner, with specificity dictated by antigens on the veto cell surface. Activated bone marrow (ABM) veto cells belong to the NK cell type lineage and veto by clonally deleting antigen-specific precursor cytotoxic T cell lymphocyte ...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.2001.1771
更新日期：2001-03-15 00:00:00
abstract：:The mechanism by which purinergic agonists modulate murine T-lymphocyte activation and proliferation was investigated. Adenosine and other compounds such as ATP and 2-chloroadenosine (ClAdo) were found to block T-cell mitogenesis induced by concanavalin A (Con A) in a dose-dependent fashion. The nonmetabolizable adeno...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(86)90199-1
更新日期：1986-08-01 00:00:00
abstract：:Neoplastic thymocytes from rat thymic lymphoma-leukemias induced by the rat-adapted Gross leukemia virus (RAGV) were analyzed for a variety of differentiation markers. The neoplasms from individual rats all expressed the antigenic phenotype MP+, W3/13+, Thy-1+, RT-1+, RT-7+, W3/25-. However, approximately two-thirds o...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90089-9
更新日期：1985-08-01 00:00:00
abstract：:The aim of our work was to study apoptosis as a possible mechanism of CD4+ lymphocyte depletion in AIDS patients and to test whether IL-12 could limit this phenomenon. As an in vitro model, we used a human IL-2-dependent Th1 clone from an uninfected individual. We found that CD4 cross-linking, obtained either by mouse...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1003
更新日期：1995-03-01 00:00:00
abstract：:The antigenic phenotype of individual PHA-induced T lymphocyte colonies was studied with a direct immunofluorescence technique using fluorescein-labeled anti-Leu-2a and anti-Leu-3a antibodies. Of the colonies grown from mononuclear peripheral blood cells 85% were Leu-3a+ (inducer/helper phenotype), 12% were Leu-2a+ (s...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(83)90235-6
更新日期：1983-10-15 00:00:00
abstract：:Various anti-Fc gamma RIII (CD16) monoclonal antibodies (mAbs) are shown here to have positive or negative modulatory effects on human NK cells. Thus, 3G8 mAb (IgG1) triggered a dose-dependent augmentation of NK activity in 67% (23/34) of individuals tested, who were designated as responders. All four IgG1 anti-CD16 m...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1006/cimm.1995.1113
更新日期：1995-07-01 00:00:00
abstract：:The loss of thymic function with age may be due to diminished numbers of T-cell progenitors and the loss of critical mediators within the thymic microenvironment. To assess the molecular changes associated with this loss, we examined transcriptomes of progressively aging mouse thymi, of different sexes and on caloric-...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2007.03.008
更新日期：2007-01-01 00:00:00
abstract：:We compared TLR responsiveness in PBMC from HIV-1-infected and uninfected individuals using the TLR agonists: TLR7 (3M-001), TLR8 (3M-002), and TLR7/8 (3M-011). Activation and maturation of plasmacytoid dendritic cells (pDC) were measured by evaluating CD86, CD40, and CD83 expression and myeloid dendritic cell (mDC) a...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2008.01.007
更新日期：2007-11-01 00:00:00
abstract：:The objective of this study was to evaluate the potential of transcutaneous immunization with tumor antigen to induce cell-mediated immunity. For this purpose, hydrophilic recombinant gp100 protein (HR-gp100) was topically applied on human intact skin in vitro, and used as a vaccine in a mouse model. We demonstrate th...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2010.09.003
更新日期：2010-01-01 00:00:00
abstract：:Experimental autoimmune encephalomyelitis was induced in a Lewis rat by sensitization with synthetic peptide GP68-86, representing the 68-86 sequence of guinea pig myelin basic protein (GPMBP). To delineate T cell determinants of GP68-86, lymph node cells from this rat were activated in culture with GP68-86 and were f...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90099-3
更新日期：1989-09-01 00:00:00
abstract：:Unconventional T cells are gaining center stage as important effector and regulatory cells that orchestrate innate and adaptive immune responses. Human Vγ9/Vδ2 T cells are amongst the best understood unconventional T cells, as they are easily accessible in peripheral blood, can readily be expanded and manipulated in v...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2015.01.008
更新日期：2015-07-01 00:00:00
abstract：:Enteric immunization with schistosome ova results in a diminished granulomatous response. This study explored a mechanism by which enteric immunization may decrease granuloma size. Granulomas from livers of acutely infected mice were dissociated and the dispersed cells were depleted of macrophages. As defined by a dir...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(87)90227-9
更新日期：1987-09-01 00:00:00
abstract：:Psychological stress evokes rapid changes to the cardiovascular and neuroendocrine systems, responses that can become habituated following repeated exposure. This study, comprising of two phases, suggests that the immune system follows a similar trend. Phase 1: 15 healthy subjects (aged between 26 and 56years) provide...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2010.02.011
更新日期：2010-01-01 00:00:00
abstract：:In this study, we demonstrate that HIV-1 Tat protein is able to induce IL-10 and TNF-alpha in human macrophages. We show that N-terminal Tat 1-45 fragment initiates the PKC pathway by acting at the membrane. Inhibition of PKC pathway, by chemical inhibitors or after PMA treatment, abolishes both IL-10 and TNF-alpha pr...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2008.06.011
更新日期：2008-01-01 00:00:00
abstract：:The cytokine interleukin 6 (IL-6) has been shown to have multiple biological activities against many cellular targets. The present studies were designed to determine whether these activities extended to the neutrophil (PMN). Initially, we investigated the ability of IL-6 to modulate PMN-mediated antibody-dependent cel...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(89)90026-9
更新日期：1989-07-01 00:00:00
abstract：:The proliferative responses of natural killer (NK) cells to 12-O-tetradecanoylphorbol-13-acetate (TPA), which directly activates protein kinase c(PKC), and to the Ca2+ ionophores A23817 and ionomycin, known to enhance the intracellular calcium, have been investigated. Highly purified large granular lymphocytes (LGL) w...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90007-x
更新日期：1988-04-15 00:00:00
abstract：:PHA stimulation of both freshly isolated and IL-2-dependent cultured T cells induced a rapid rise in intracytoplasmic calcium concentration. Chelation of extracellular calcium with EGTA resulted in a failure of PHA to induce a rise in intracytoplasmic calcium, resulting in the fresh T cells in an inhibition of IL-2 pr...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(88)90060-3
更新日期：1988-01-01 00:00:00
abstract：:Recent evidence highlighted the role of Toll-like receptors (TLRs) as key recognition structures of the innate immune system. The activation of TLRs initiates the production of inflammatory cytokines, chemokines, tissue destructive enzymes, and type I interferons. In addition, TLR signalling plays an important role in...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2005.04.018
更新日期：2005-02-01 00:00:00
abstract：:Optical imaging is a valuable tool to visualise biological processes in the context of the tissue. Each imaging modality provides the biologist with different types of information - cell dynamics and migration over time can be tracked with time-lapse imaging (e.g. intra-vital imaging); an overview of whole tissues can...
journal_title：Cellular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.cellimm.2019.103946
更新日期：2019-10-01 00:00:00
abstract：:Nickel (Ni) can cause delayed-type hypersensitivity reactions, which are thought to be mediated by the accumulation of T cells into inflamed skin. Accumulated T cells at the developmental stages in metal allergy are poorly characterized because a suitable animal model has not been established. To investigate the accum...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/j.cellimm.2013.07.010
更新日期：2013-07-01 00:00:00
abstract：:It has been reported that a muramyl dipeptide (MDP) treatment regimen (200 micrograms MDP per mouse, Days -4, -3, -2, and -1) that, given prophylactically, affords protection against several infectious agents also induces lymph node hyperplasia, lymph node cell (LNC) hyperresponsiveness to mitogens, and spleen cell hy...
journal_title：Cellular immunology
pub_type： 杂志文章
doi：10.1016/0008-8749(85)90248-5
更新日期：1985-04-01 00:00:00
