Abstract:
BACKGROUND:Adipose tissue abundance relies partly on the factors that regulate adipogenesis, i.e. proliferation and differentiation of adipocytes. While components of the transcriptional program that initiates adipogenesis is well-known, the importance of microRNAs in adipogenesis is less well studied. We thus set out to investigate whether miRNAs would be actively modulated during adipogenesis and obesity. METHODS:Several models exist to study adipogenesis in vitro, of which the cell line 3T3-L1 is the most well known, albeit not the most physiologically appropriate. Thus, as an alternative, we produced EXIQON microarray of brown and white primary murine adipocytes (prior to and following differentiation) to yield global profiles of miRNAs. RESULTS:We found 65 miRNAs regulated during in vitro adipogenesis in primary adipocytes. We evaluated the similarity of our responses to those found in non-primary cell models, through literature data-mining. When comparing primary adipocyte profiles, with those of cell lines reported in the literature, we found a high degree of difference in 'adipogenesis' regulated miRNAs suggesting that the model systems may not be accurately representing adipogenesis. The expression of 10 adipogenesis-regulated miRNAs were studied using real-time qPCR and then we selected 5 miRNAs, that showed robust expression, were profiled in subcutaneous adipose tissue obtained from 20 humans with a range of body mass indices (BMI, range = 21-48, and all samples have U133+2 Affymetrix profiles provided). Of the miRNAs tested, mir-21 was robustly expressed in human adipose tissue and positively correlated with BMI (R2 = 0.49, p < 0.001). CONCLUSION:In conclusion, we provide a preliminary analysis of miRNAs associated with primary cell in vitro adipogenesis and demonstrate that the inflammation-associated miRNA, mir-21 is up-regulated in subcutaneous adipose tissue in human obesity. Further, we provide a novel transcriptomics database of EXIQON and Affymetrix adipocyte profiles to facilitate data mining.
journal_name
BMC Endocr Disordjournal_title
BMC endocrine disordersauthors
Keller P,Gburcik V,Petrovic N,Gallagher IJ,Nedergaard J,Cannon B,Timmons JAdoi
10.1186/1472-6823-11-7subject
Has Abstractpub_date
2011-03-22 00:00:00pages
7issn
1472-6823pii
1472-6823-11-7journal_volume
11pub_type
杂志文章abstract:UNLABELLED: BACKGROUND:Hypoglycaemia is a serious adverse effect of antidiabetic drug therapy. We aimed to determine incidence rates of hypoglycaemia in type-2 diabetic patients and identify predictors of hypoglycaemia when treatment is intensified. METHODS:DiaRegis is a prospective German registry that follows 3810 ...
journal_title:BMC endocrine disorders
pub_type: 杂志文章
doi:10.1186/1472-6823-12-23
更新日期:2012-10-17 00:00:00
abstract:BACKGROUND:We quantified, among diabetic adults, the frequency, costs, and factors associated with visits to the emergency department (EDs) and subsequent hospitalizations for acute hypoglycaemic and hyperglycaemic events. METHODS:We included adults with diabetes residing in the Milan Agency for Health Protection betw...
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journal_title:BMC endocrine disorders
pub_type: 杂志文章,meta分析,评审
doi:10.1186/s12902-017-0209-z
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abstract:BACKGROUND:Anti-Müllerian hormone (AMH) is now considered the best serum biomarker of ovarian reserve, while basal sex hormones are classic markers used for assessing ovarian reserve. The interaction between AMH and sex hormones are complicated and not sufficiently addressed. In this study, we took diminished ovarian r...
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
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abstract:BACKGROUND:The objective was to examine how the indigenous naming, indigenous self-diagnosis and management of diabetes evolved with awareness in order to develop a socially oriented theoretical model for its care. METHODS:The data was collected through a one-year extended participant observation in Bafut, a rural hea...
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pub_type: 杂志文章
doi:10.1186/1472-6823-9-5
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abstract:BACKGROUND:Irisin is a myokine that leads to increased energy expenditure by stimulating the browning of white adipose tissue. We aimed to investigate the association of serum irisin levels with metabolic parameters in middle aged Chinese population. METHODS:The study was based on a cross-sectional analysis of data fr...
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pub_type: 杂志文章
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更新日期:2019-12-27 00:00:00
abstract:BACKGROUND:The rising prevalence of type 2 diabetes underlines the importance of secondary strategies for the prevention of target organ damage. While access to diabetes education centers and diabetes intensification management has been shown to improve blood glucose control, these services are not available to all tha...
journal_title:BMC endocrine disorders
pub_type: 杂志文章
doi:10.1186/1472-6823-9-13
更新日期:2009-05-09 00:00:00
abstract::Hypopituitarism is deficiency of one or more pituitary hormones, of which adrenocorticotrophic hormone (ACTH) deficiency is the most serious and potentially life-threatening. It may occur in isolation or, more commonly as part of more widespread pituitary failure. Diagnosis requires demonstration of subnormal cortisol...
journal_title:BMC endocrine disorders
pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
doi:10.1186/1472-6823-13-20
更新日期:2013-06-22 00:00:00
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
doi:10.1186/s12902-017-0201-7
更新日期:2017-08-15 00:00:00
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
doi:10.1186/1472-6823-12-4
更新日期:2012-07-10 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12902-020-0502-0
更新日期:2020-02-11 00:00:00
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pub_type: 杂志文章
doi:10.1186/s12902-020-00597-2
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abstract:BACKGROUND:Benign thyroid nodules (BTN) are frequently diagnosed as papillary thyroid carcinoma (PTC), leading to unnecessary treatment. We found that plasma lncRNA DLG1-AS1 was upregulated in PTC patients but not in BTN patients and healthy controls. METHODS:In this study DLG1-AS1 and miR-199a-3p in plasma of both PT...
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pub_type: 杂志文章
doi:10.1186/s12902-019-0440-x
更新日期:2019-11-12 00:00:00
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
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doi:10.1186/s12902-018-0272-0
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
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更新日期:2014-06-09 00:00:00
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
doi:10.1186/1472-6823-8-9
更新日期:2008-07-31 00:00:00
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
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更新日期:2018-05-29 00:00:00
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
doi:10.1186/s12902-016-0107-9
更新日期:2016-05-23 00:00:00
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
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更新日期:2020-08-24 00:00:00
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
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更新日期:2014-03-08 00:00:00
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journal_title:BMC endocrine disorders
pub_type: 杂志文章
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