Altered expression of DLG1-AS1 distinguished papillary thyroid carcinoma from benign thyroid nodules.

Abstract:

BACKGROUND:Benign thyroid nodules (BTN) are frequently diagnosed as papillary thyroid carcinoma (PTC), leading to unnecessary treatment. We found that plasma lncRNA DLG1-AS1 was upregulated in PTC patients but not in BTN patients and healthy controls. METHODS:In this study DLG1-AS1 and miR-199a-3p in plasma of both PTC patients and BTN patients were detected by qPCR. ROC curve analysis was performed for diagnostic analysis. Overexpression experiments were performed to analyze the interaction between DLG1-AS1 and miR-199a-3p. CCK-8 assay was performed to analyze cell proliferation. RESULTS:In this study, upregulation of DLG1-AS1 distinguished PTC patients from BTN patients and healthy controls. Plasma miR-199a-3p was downregulated in PTC patients compared with healthy controls and BTN patients. Plasma levels of miR-199a-3p were inversely correlated in PTC patients, but not in BTN patients and healthy controls. miR-199a-3p overexpression failed to significantly affect DLG1-AS1, while DLG1-AS1 overexpression resulted in downregulated miR-199a-3p, In addition, DLG1-AS1 overexpression promoted the proliferation of PTC cells. miR-199a-3p overexpression played an opposite role and attenuated the effects of DLG1-AS1 overexpression. CONCLUSIONS:Therefore, DLG1-AS1 may promote PTC by downregulating miR-199a-3p.

journal_name

BMC Endocr Disord

journal_title

BMC endocrine disorders

authors

He T,Wang H,Sun J,Wu J,Gong F,Li S,Wang H,Li Y

doi

10.1186/s12902-019-0440-x

subject

Has Abstract

pub_date

2019-11-12 00:00:00

pages

122

issue

1

issn

1472-6823

pii

10.1186/s12902-019-0440-x

journal_volume

19

pub_type

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