Interactive contributions of intracellular calcium and protein phosphatases to massed-trials learning deficits in Hermissenda.

Abstract:

:Using Hermissenda as subjects, massed-trials training deficits were examined. Associative pairings of light and rotation induced a progressively greater conditioned foot contraction in response to light as the intertrial interval (ITI) was extended (up to 8 min). In contrast, a short ITI (30 s) produced no evidence of learning. In a corresponding in vitro conditioning experiment that mimicked training of the intact animal, facilitation of neuronal excitability in the animal's B photoreceptors paralleled the results obtained in vivo. Imaging of intracellular Ca2+ using Fura-2 indicated that Ca2+ levels remained elevated during short ITIs. This Ca2+ accumulation appears to induce activation of protein phosphatases because normal facilitation of the B photoreceptors was induced with a short ITI if training occurred in the presence of a phosphatase inhibitor. These results suggest that intracellular Ca2+ and protein phosphatases contribute interactively to the kinetics of memory formation and provide evidence that an accumulation of intracellular Ca2+ across training trials may impede memory formation.

journal_name

Behav Neurosci

journal_title

Behavioral neuroscience

authors

Muzzio IA,Ramirez RR,Talk AC,Matzel LD

doi

10.1037//0735-7044.113.1.103

subject

Has Abstract

pub_date

1999-02-01 00:00:00

pages

103-17

issue

1

eissn

0735-7044

issn

1939-0084

journal_volume

113

pub_type

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