Novel and established applications of microbial polysaccharides.

abstract：:The well-studied antigen-combining sites of antibody molecules hold considerable promise as a model system for the design of bioactive peptides. These small, immunoglobulin-derived peptides can be used in the development of alternative treatments for disease and in diagnostic strategies. The general principles derived...

journal_title：Trends in biotechnology

authors： Dougall WC,Peterson NC,Greene MI

更新日期：1994-09-01 00:00:00

abstract：:Carotenoids are micronutrients important for human health. The continuous improvements in clustered regularly interspaced short palindromic repeats (CRISPR)-based genome-editing techniques make rapid, DNA/transgene-free and targeted multiplex genetic modification a reality, thus promising to accelerate the breeding an...

journal_title：Trends in biotechnology

authors： Zheng X,Kuijer HNJ,Al-Babili S

更新日期：2020-12-29 00:00:00

abstract：:With the ultimate aim to construct a living cell, bottom-up synthetic biology strives to reconstitute cellular phenomena in vitro - disentangled from the complex environment of a cell. Recent work towards this ambitious goal has provided new insights into the mechanisms governing life. With the fast-growing library of...

journal_title：Trends in biotechnology

authors： Göpfrich K,Platzman I,Spatz JP

更新日期：2018-09-01 00:00:00

abstract：:The ability to detect single-base changes is of fundamental importance in molecular genetics. This is particularly true in human genetics, where interest in linking mutations of identified genes to particular disease phenotypes is most intense, and where a demand exists for clinical diagnosis of defined mutations. In ...

journal_title：Trends in biotechnology

authors： Prosser J

更新日期：1993-06-01 00:00:00

abstract：:Since its invention at the beginning of the 1990s, antibody phage display has revolutionized the generation of monoclonal antibodies and their engineering. It is now possible to create antibodies binding to any chosen target antigen without the use of laboratory animals or hybridomas, in a system that completely by-pa...

journal_title：Trends in biotechnology

authors： Hoogenboom HR

更新日期：1997-02-01 00:00:00

abstract：:Selective delivery of lethal levels of drugs to tumors, without concomitant damage to normal tissues, is a major challenge in cancer chemotherapy. Prodrugs used in conjunction with enzyme-monoclonal antibody conjugates that can target tumors and convert prodrugs to their active drug forms in situ, offer exceptional pr...

journal_title：Trends in biotechnology

authors： Huennekens FM

更新日期：1994-06-01 00:00:00

abstract：:Diabetes affects 150 million people worldwide and results from abnormal function of pancreatic islets. The scarcity of human tissue donors has focused interest in developing renewable sources of insulin-producing cells appropriate for engraftment. Advances in stem cell technology and transdifferentiation techniques ha...

journal_title：Trends in biotechnology

authors： Orive Arroyo G,Hernánedez RM,Gascón AR,Igartua M,Pedraz JL

更新日期：2003-07-01 00:00:00

abstract：:Feedstocks that deserve serious consideration for fuels and chemicals are sugarcane, corn, trees and algae. Commercialization of biomass refining is imminent but the wild claims of those who think that bioenergy can replace much of our dependence on foreign oil are appalling. It is naive to view biomass as the panacea...

journal_title：Trends in biotechnology

authors： Bungay HR

更新日期：2004-02-01 00:00:00

abstract：:Small molecules are defined as low molecular weight organic compounds (typically <1000 Da), which could be either natural or artificial. Because established imaging methods are not able to selectively detect the positions, concentrations and structures of small molecules in biological samples, new methods have been de...

journal_title：Trends in biotechnology

authors： Svatos A

更新日期：2010-08-01 00:00:00

abstract：:Life science in the 21st century is developing rapidly through the structural analysis of biomolecules, the completion of the human genome sequence and the analysis of transcriptomes. The mouse transcriptome has been comprehensively analyzed using a gene discovery approach to collect full-length cDNA (FL-cDNA) clones....

journal_title：Trends in biotechnology

authors： Hayashizaki Y,Kanamori M

更新日期：2004-04-01 00:00:00

abstract：:Musculoskeletal diseases are increasing the prevalence of physical disability worldwide. Within the body, musculoskeletal soft and hard tissues integrate through specific multitissue transitions, allowing for body movements. Owing to their unique compositional and structural gradients, injuries challenge the native in...

journal_title：Trends in biotechnology

authors： Calejo I,Costa-Almeida R,Reis RL,Gomes ME

更新日期：2020-01-01 00:00:00

abstract：:Biosensor technology can offer the livestock industry new types of monitoring and measuring devices of which the specificity, sensitivity, reproducibility, speed and ease of use exceed the current technology. Biosensors can be applied to the detection and identification of infectious diseases in livestock, contaminant...

journal_title：Trends in biotechnology

authors： Velasco-Garcia MN,Mottram T

更新日期：2001-11-01 00:00:00

abstract：:Protein engineers traditionally rely on amino acid substitutions to alter the functional properties of biomacromolecules, yet have largely overlooked the potential benefits of reorganizing the polypeptide chain of a protein by circular permutation (CP). By connecting the native protein termini via a covalent linker an...

journal_title：Trends in biotechnology

authors： Yu Y,Lutz S

更新日期：2011-01-01 00:00:00

abstract：:Metabolomics embraces several strategies that aim to quantify cell metabolites in order to increase our understanding of how metabolite levels and interactions influence phenotypes. Metabolic footprinting represents a niche within metabolomics, because it focuses on the analysis of extracellular metabolites. Although ...

journal_title：Trends in biotechnology

authors： Mapelli V,Olsson L,Nielsen J

更新日期：2008-09-01 00:00:00

abstract：:We describe factors promoting the exploration of locally sourced probiotics, targeting local populations to balance human needs and market opportunities. This would be particularly beneficial for people in developing countries, who generally lack access to affordable probiotics and are often exposed to poor hygiene co...

journal_title：Trends in biotechnology

authors： Sybesma W,Kort R,Lee YK

更新日期：2015-04-01 00:00:00

abstract：:Microalgal production technologies are seen as increasingly attractive for bioenergy production to improve fuel security and reduce CO(2) emissions. Photosynthetically derived fuels are a renewable, potentially carbon-neutral and scalable alternative reserve. Microalgae have particular promise because they can be prod...

journal_title：Trends in biotechnology

authors： Larkum AW,Ross IL,Kruse O,Hankamer B

更新日期：2012-04-01 00:00:00

abstract：:The best long-term prospects for the reduction in the number of deaths due to cancer lie with screening and prevention rather than with therapy of the advanced disease. Screening efficiency will be improved by the identification of individuals at inherited high risk of cancer. Understanding the mechanism of predisposi...

journal_title：Trends in biotechnology

authors： Ponder BA

更新日期：1990-04-01 00:00:00

abstract：:The discovery of targets that are sufficiently robust to yield marketable therapeutics is an enormous challenge. Through the years, several approaches have been used with varying degrees of success. These include target-independent screening of tumor-derived cell lines (disease-dependent), reductionist approaches to i...

journal_title：Trends in biotechnology

authors： Ryan TE,Patterson SD

更新日期：2002-12-01 00:00:00

abstract：:Stem cell differentiation is regulated by the complex interplay of multiple parameters, including adhesive intercellular interactions, cytoskeletal and extracellular matrix remodeling, and gradients of agonists and antagonists that individually and collectively vary as a function of spatial locale and temporal stages ...

journal_title：Trends in biotechnology

authors： Kinney MA,McDevitt TC

更新日期：2013-02-01 00:00:00

abstract：:Dual-use research, which results in knowledge that can be used for both good and ill, has become increasingly accessible in the internet age to both scientists and the general public. Here, we outline some major milestones for dual-use policy and present three vignettes that highlight contemporary dual-use issues in b...

journal_title：Trends in biotechnology

authors： Getz LJ,Dellaire G

更新日期：2018-12-01 00:00:00

abstract：:In Europe, in light of ethical, political and commercial pressure, every effort should be made to replace animals with alternatives (e.g. in vitro models), to reduce the number of animals used in experiments to a minimum and to refine current testing strategies in a way that ensures animals undergo minimum pain and di...

journal_title：Trends in biotechnology

authors： Kroeger M

更新日期：2006-08-01 00:00:00

abstract：:Opinions on what synthetic biology actually is range from a natural extension of genetic engineering to a new manufacturing paradigm. It offers, for the first time in the life sciences, rational design and engineering standardisation. It could address problems across a broad spectrum of human concerns, including energ...

journal_title：Trends in biotechnology

authors： Philp JC,Ritchie RJ,Allan JE

更新日期：2013-05-01 00:00:00

abstract：:Techniques such as positron-emission tomography, single-photon-emission computed tomography, functional magnetic-resonance imaging and magnetoencephalography permit the observation of biological processes in the brain in a noninvasive manner. They have yielded new insights into the biological interrelations of sensory...

journal_title：Trends in biotechnology

authors： Müller-Gärtner HW

更新日期：1998-03-01 00:00:00

abstract：:Although there is still some skepticism in the biological community regarding the value and significance of quantitative computational modeling, important steps are continually being taken to enhance its accessibility and predictive power. We view these developments as essential components of an emerging 'respectful m...

journal_title：Trends in biotechnology

authors： Tsigkinopoulou A,Baker SM,Breitling R

更新日期：2017-06-01 00:00:00

abstract：:Marine natural products (NP) are unanimously acknowledged as the 'blue gold' in the urgent quest for new pharmaceuticals. Although corals are among the marine organisms with the greatest diversity of secondary metabolites, growing evidence suggest that their symbiotic bacteria produce most of these bioactive metabolit...

journal_title：Trends in biotechnology

authors： Leal MC,Calado R,Sheridan C,Alimonti A,Osinga R

更新日期：2013-10-01 00:00:00

abstract：:The glycosylation profile of a recombinant glycoprotein can not only significantly affect its therapeutic profile, but is also extremely sensitive to cell-culture and purification conditions. To define glycosylation patterns and to ensure consistency of recombinant glycoproteins among different preparations requires h...

journal_title：Trends in biotechnology

authors： Parekh RB,Patel TP

更新日期：1992-08-01 00:00:00

abstract：:Discussions on research quality and reproducibility are appearing in the pages of scientific journals with heightened significance and gaining media attention. Many institutions have developed guidelines to address the topic of quality in basic research, but questions remain about how best to implement and monitor com...

journal_title：Trends in biotechnology

authors： Riedl DH,Dunn MK

更新日期：2013-10-01 00:00:00

abstract：:The genome-sequencing projects are providing a detailed 'parts list' of life. A key to comprehending this list is understanding the function of each gene and each protein at various levels. Sequence-based methods for function prediction are inadequate because of the multifunctional nature of proteins. However, just kn...

journal_title：Trends in biotechnology

authors： Skolnick J,Fetrow JS

更新日期：2000-01-01 00:00:00

abstract：:The development of yeast artificial chromosome (YAC) cloning vectors capable of carrying several hundred kilobase-pairs of DNA insert has greatly facilitated the study of complex genomes, and the cloning of large genes as single fragments. In addition, the ability to manipulate YAC sequences by homologous recombinatio...

journal_title：Trends in biotechnology

authors： Anand R

更新日期：1992-01-01 00:00:00

abstract：:In situ 3D printing is an emerging technique designed for patient-specific needs and performed directly in the patient's tissues in the operating room. While this technology has progressed rapidly, several improvements are needed to push it forward for widespread utility, including ink formulations and optimization fo...

journal_title：Trends in biotechnology

authors： Agostinacchio F,Mu X,Dirè S,Motta A,Kaplan DL

更新日期：2020-12-02 00:00:00