Abstract：
:We have examined the stability and sources of variation within the nociceptive model of rat hind paw withdrawal from an under-glass radiant stimulus (Hargreaves et al., 1988) using a system where stimulus intensity and floor temperature can be controlled and reproducibly changed. The current study demonstrates that: (i) increased stimulus intensity with a fixed surface temperature is associated with a monotonic decrease in mean response latency and its variance; (ii) for a fixed stimulus intensity, the mean paw withdrawal latency and variance increased as the glass floor temperature is lowered from 30 degrees C to room temperature (25 degrees C). Using subcutaneously-implanted thermocouples and a 30 degrees C glass surface, the subcutaneous paw temperature observed at an interval corresponding to the time at which the animal displayed a paw withdrawal did not differ across multiple heating rates (41-42.5 degrees C). This finding is in agreement with human studies of pain thresholds and C-fiber activity. These studies emphasize the importance of maintaining a fixed surface temperature to reduce experimental variability and the utility of this apparatus across multiple stimulus intensities to define agonist efficacy.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Dirig DM,Salami A,Rathbun ML,Ozaki GT,Yaksh TLdoi
10.1016/s0165-0270(97)00097-6subject
Has Abstractpub_date
1997-10-03 00:00:00pages
183-91issue
2eissn
0165-0270issn
1872-678Xpii
S0165-0270(97)00097-6journal_volume
76pub_type
杂志文章abstract：:Although dopamine (DA) translocation by the DA transporter (DAT) requires Na+, a role for Na+ in the DA recognition step in the translocation cycle has been questioned. Thus, when binding techniques were used to indirectly measure the affinity of DA for DAT via its potency in inhibiting cocaine analog binding, no stim...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00230-9
更新日期：2003-12-30 00:00:00
abstract：:Beamforming offers a way to estimate the solution to the inverse problem in EEG and MEG but is also known to perform poorly in the presence of highly correlated sources, e.g. during binaural auditory stimulation, when both left and right primary auditory cortices are activated simultaneously. Surface Laplacian, or the...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.05.001
更新日期：2013-08-15 00:00:00
abstract：:A double pipette system for local, controlled drug infusion is presented. Two concentric pipettes can be manipulated separately and pressurized independently by a designated double holder. The inner pipette is loaded with the desirable solution (drug), and functions as a source, while the outer one is a sink. This giv...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(03)00099-2
更新日期：2003-07-15 00:00:00
abstract：:Brain-computer interface (BCI) technology has attracted significant attention over recent decades, and has made remarkable progress. However, BCI still faces a critical hurdle, in that performance varies greatly across and even within subjects, an obstacle that degrades the reliability of BCI systems. Understanding th...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2015.01.033
更新日期：2015-03-30 00:00:00
abstract：:Senile plaques (SP) are the most characteristic neuropathologic lesions of Alzheimer's disease (AD) and studies of plaque cortical distribution, density, and morphology may lead to new information about the origin and pathogenesis of this disease. We have developed an automated computer image analysis program to detec...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(94)90128-7
更新日期：1994-06-01 00:00:00
abstract：BACKGROUND:How experience and individuality shape action selection remains a major question in neuroscience. Visually-evoked escape behavior within Drosophila melanogaster provides a robust model to study these mechanisms within neural circuits but requires novel assays to circumvent limitations of current behavior ass...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.04.019
更新日期：2018-07-01 00:00:00
abstract：:Much interest has been shown in the use of multi-template reverse transcription-polymerase chain reaction (RT-PCR) as a quantitative instrument for low-abundance mRNAs. A desire to achieve finely-graded quantification of the stress- and hormone-related regulation of one splicing decision in an ion channel gene motivat...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00015-8
更新日期：2002-04-15 00:00:00
abstract：:Binding of the cocaine analog 3 beta-(4-[125I]iodophenyl)tropane-2 beta-carboxylic acid isopropyl ester ([125I]RTI-121) to filters was studied in order to assess its contribution to labeling dopamine transporters on rat striatal synaptosomal membranes in filtration assays. Filter binding (FB) decreased with increasing...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(97)00070-8
更新日期：1997-08-22 00:00:00
abstract：:NREM phases of sleep in vertebrates are characterized by slow waves. Crayfish also sleeps while lying on one side on the surface of the water. At this time the numerous spikes on an almost flat base line generated by the brain when alert are replaced by slow waves of 15-20 Hz. In this work, we conducted experiments to...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.01.025
更新日期：2007-05-15 00:00:00
abstract：:Diffusion Magnetic Resonance Imaging (dMRI) is one of the most important contemporary non-invasive modalities for probing tissue structure at the microscopic scale. The majority of dMRI techniques employ standard single diffusion encoding (SDE) measurements, covering different sequence parameter ranges depending on th...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108989
更新日期：2021-01-15 00:00:00
abstract：:Images of Golgi impregnated neurons from different laminae of the human and rat dorsal horns were subjected to a quantitative analysis to support the Rexed's laminar scheme in mammals. Four methods of fractal analysis were performed in the proceedings: box-counting, mass-radius, cumulative intersection, and vectorized...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2005.02.009
更新日期：2005-08-15 00:00:00
abstract：:Fast-scan cyclic voltammetry (FSCV) at a carbon-fiber microelectrode (CFM) provides exquisite temporal and spatial resolution for monitoring brain chemistry. The utility of this approach has recently been demonstrated by measuring sub-second dopamine changes associated with behavior. However, one drawback is the cable...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.04.043
更新日期：2004-12-30 00:00:00
abstract：:Superpositioning of multiple 3-dimensional digital volumes is essential to extending neuroimaging capabilities from single-subject analysis to group evaluation. In this study, 6 rat heads were cryoplaned and digital images of the specimen blockface were organized into data volumes. The midline plane of each brain guid...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90007-e
更新日期：1993-11-01 00:00:00
abstract：BACKGROUND:The accuracy of source reconstruction depends on the spatial configuration of the neural sources underlying encephalographic signals, the temporal distance of the source activity, the level and structure of noise in the recordings, and - of course - on the employed inverse method. This plenitude of factors r...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2018.02.016
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:In the era of computer-assisted diagnostic tools for various brain diseases, Alzheimer's disease (AD) covers a large percentage of neuroimaging research, with the main scope being its use in daily practice. However, there has been no study attempting to simultaneously discriminate among Healthy Controls (HC)...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.12.010
更新日期：2018-05-15 00:00:00
abstract：:Recombinant proteins are routinely expressed in heterologous expression systems such as human embryonic kidney 293 (HEK 293) cells. The efficiency of the expression is critical when the expressed protein must be characterized at the single-cell level. Here we describe a simple method by which the protein expression ef...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2004.09.009
更新日期：2005-03-15 00:00:00
abstract：:A dimensionally accurate clear model of the human scala tympani has been produced to evaluate the insertion and position of clinically applied intracochlear electrodes for electrical stimulation. Replicates of the human scala tympani were made from low melting point metal alloy (LMA) and from polymethylmeth-acrylate (...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(95)00116-6
更新日期：1996-01-01 00:00:00
abstract：BACKGROUND:Accurate and reliable measurement of leukoaraiosis, or MR-detected white, matter hyper-intensity (WMH) burden in subjects with acute ischemic stroke (AIS) is important for, ongoing research studies and future models of risk and outcome prediction, but the presence of a, cerebral infarct may complicate measur...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.10.009
更新日期：2014-01-15 00:00:00
abstract：BACKGROUND:Recording and manipulating neural activity in awake behaving animal models requires long-term implantation of cranial implants that must address a variety of design considerations, which include preventing infection, minimizing tissue damage, mechanical strength of the implant, and MRI compatibility. NEW ME...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.12.011
更新日期：2015-02-15 00:00:00
abstract：BACKGROUND:Experimental stroke studies use multiple techniques to evaluate histopathological damage. Unfortunately, sensitivity and reproducibility of these techniques are poorly characterized despite pivotal influence on results. METHOD:The present study compared several quantification methods to differentiate betwee...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.11.001
更新日期：2015-01-30 00:00:00
abstract：:Controlled expression of proteins is a key experimental approach to a deeper understanding of the molecular basis of neuronal function. Here we evaluate the HSV-1 (herpes simplex virus) amplicon vector for gene delivery into the brains of living rats. We demonstrate that HSV-1 amplicon vectors expressing enhanced gree...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(02)00262-5
更新日期：2002-12-15 00:00:00
abstract：BACKGROUND:Frequency domain Granger causality measures have been proposed and widely applied in analyzing rhythmic neurophysiological and biomedical signals. Almost all these measures are based on linear time domain regression models, and therefore can only detect linear causal effects in the frequency domain. NEW MET...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.01.013
更新日期：2014-03-30 00:00:00
abstract：BACKGROUND:Virtual reality-based neuropsychological assessments proffer the potential to address the limited ecological validity of pen-and-paper measures of memory. NEW METHOD:To investigate the construct validity of a newly developed virtual reality-based multiple errands task, the Virtual Environment Grocery Store ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.07.027
更新日期：2017-11-01 00:00:00
abstract：:Amyloid-beta (Abeta) deposits are one of the hallmarks of the neuropathological degeneration observed in Alzheimer's disease (AD) and Abeta concentrations have been reported to vary in different brain regions of AD patients. Abeta is produced by the sequential cleavage of amyloid precursor protein (APP) by beta-secret...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.05.001
更新日期：2006-10-30 00:00:00
abstract：:An important parameter often investigated in the characterization of cortical functional organization is the areal extent of functional modules. Because it allows the visualization of functional modules with high spatial resolution in a noninvasive way to the cortex, intrinsic signal optical imaging (ISI) can be emplo...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：
更新日期：1996-09-01 00:00:00
abstract：:The use of paper impregnated with Bromphenol blue for recording normal rat footprints is described. Because paper provides a better grip than X-ray film for the paws of the rats, the resulting tracks make manual scoring easier and more accurate. In addition, the cost of this technique is considerably lower. ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(88)90173-2
更新日期：1988-07-01 00:00:00
abstract：:A hydraulically driven, digitally servo-controlled multi-axes rotary chair is described. This device generates motion profiles with the subjects head in the center of rotation mainly in order to adequately stimulate the semicircular canals which are sensitive sensors for angular accelerations. This newly developed app...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(93)90108-4
更新日期：1993-08-01 00:00:00
abstract：BACKGROUND:Sympathetic nerve wiring in the mammalian heart has remained largely unexplored. Resolving the wiring diagram of the cardiac sympathetic network would help establish the structural underpinnings of neurocardiac coupling. NEW METHOD:We used two-photon excitation fluorescence microscopy, combined with a compu...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.09.005
更新日期：2014-01-15 00:00:00
abstract：BACKGROUND:No satisfactory neurophysiological test for nociceptive afferents is available to date. Laser stimuli present risks of skin damage, whilst electrical stimulation through specially designed electrodes is not selective enough. NEW METHOD:We present a new electrode designed according to critical issues identif...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.08.032
更新日期：2018-01-01 00:00:00
abstract：BACKGROUND:Photothrombosis is a minimally invasive method for induction of cortical ischemia. However, different ways of applying some methods to assess photothrombosis-induced damage need to be developed. NEW METHODS:We applied the tongue protrusion test and H&E staining of brain sections to detect ischemic damage af...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2019.108457
更新日期：2020-01-01 00:00:00
