Clinical evaluation of the BDProbeTec ET system for rapid detection of Mycobacterium tuberculosis.

abstract：:In tests on specimens of dried blood, saliva, and urine from 55 human immunodeficiency virus (HIV)-seropositive and 55 HIV-seronegative patients, an immunoglobulin G capture enzyme immunoassay for the detection of antibodies to HIV types 1 and 2, GACELISA, gave 109 of 110, 109 of 109, and 109 of 110 correct results, r...

journal_title：Journal of clinical microbiology

authors： Thongcharoen P,Wasi C,Louisirirotchanakul S,Parry J,Connell J,Mortimer P

更新日期：1992-12-01 00:00:00

abstract：:Although it has been recommended that photochromogenicity of mycobacteria should be tested after incubation at 25 to 30 degrees C for 2 weeks (Wayne et al., Int. J. Syst. Bacteriol. 24:412-419, 1974), our results indicate that photochromogenicity should be determined at 37 degrees C in the dark and under continuous ex...

journal_title：Journal of clinical microbiology

authors： Tsukamura M

更新日期：1981-08-01 00:00:00

abstract：:Herpes simplex virus (HSV) infections of the central nervous system (CNS) are associated with significant morbidity and mortality rates in children. This study assessed the impact of a direct HSV (dHSV) PCR assay on the time to result reporting and the duration of acyclovir therapy for children with signs and symptoms...

journal_title：Journal of clinical microbiology

authors： Van TT,Mongkolrattanothai K,Arevalo M,Lustestica M,Dien Bard J

更新日期：2017-05-01 00:00:00

abstract：:Many parts of the Salmonella rfb gene clusters which are responsible for biosynthesis of the oligosaccharide-repeating units of the O-antigenic lipopolysaccharide have recently been cloned and sequenced. On the basis of this knowledge, three sets of nucleotide primers were selected to target defined regions of the abe...

journal_title：Journal of clinical microbiology

authors： Luk JM,Kongmuang U,Reeves PR,Lindberg AA

更新日期：1993-08-01 00:00:00

abstract：:COVID-19-associated pulmonary aspergillosis (CAPA) was recently reported as a potential infective complication affecting critically ill patients with acute respiratory distress syndrome following severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, with incidence rates varying from 8 to 33% dependin...

journal_title：Journal of clinical microbiology

authors： Borman AM,Palmer MD,Fraser M,Patterson Z,Mann C,Oliver D,Linton CJ,Gough M,Brown P,Dzietczyk A,Hedley M,McLachlan S,King J,Johnson EM

更新日期：2020-12-17 00:00:00

abstract：:The aim of this study was to determine if gastroenteric viruses were present on surfaces and equipment in a pediatric primary immunodeficiency unit (PPIU) by environmental sampling using swabs and subsequent nucleic acid extraction and reverse transcriptase PCR assays. A PPIU was chosen, and 11 swabs were taken at the...

journal_title：Journal of clinical microbiology

authors： Gallimore CI,Taylor C,Gennery AR,Cant AJ,Galloway A,Iturriza-Gomara M,Gray JJ

更新日期：2006-02-01 00:00:00

abstract：:The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers in the polymerase chain reaction. Genomic DNA from each of 20 human isolates of A. actinomycetemcomitans was successfully amplified in a thermal cycle...

journal_title：Journal of clinical microbiology

authors： Preus HR,Haraszthy VI,Zambon JJ,Genco RJ

更新日期：1993-10-01 00:00:00

abstract：:The EasyNAT assay was evaluated for the detection of tuberculosis in sputum smears from presumptive pulmonary tuberculosis (TB) patients in an African high-TB and high-HIV setting. The sensitivity of the EasyNAT assay was 66.7%, and the specificity and positive predictive value were 100% for the culture-positive patie...

journal_title：Journal of clinical microbiology

authors： Mhimbira FA,Bholla M,Sasamalo M,Mukurasi W,Hella JJ,Jugheli L,Reither K

更新日期：2015-04-01 00:00:00

abstract：:We compared anaerobic lytic (AL) and pediatric aerobic resin-containing (Peds Plus/F) blood culture media for the isolation of Salmonella enterica serotype Typhi or Paratyphi A from children. The yields from AL and Peds Plus/F media were the same with equal volumes of blood, but recovery was faster from AL medium than...

journal_title：Journal of clinical microbiology

authors： Reller ME,Zaidi AK,Sultana S,Azeem S,Hanif B,Qureshi S,Hasan R,Bhutta Z,Akhter R,Goldmann DA

更新日期：2009-01-01 00:00:00

abstract：:Many truly human immunodeficiency virus (HIV) antibody-negative serum samples may be unnecessarily subjected to costly and time-consuming Western blots (immunoblots). An investigation was undertaken to evaluate the efficiency of using a recombinant protein-based enzyme immunosorbent assay (EIA; Cambridge BioScience [C...

journal_title：Journal of clinical microbiology

authors： Lepine DG,Neumann PW,Frenette SL,O'Shaughnessy MV

更新日期：1990-06-01 00:00:00

abstract：:Infection of calves with bovine herpesvirus 1 (BHV-1) results in transient immunosuppression that may lead to bacterium-induced pneumonia and, occasionally, death. Although sensory neurons in the trigeminal ganglia (TG) are the primary site of BHV-1 latency, viral genomes are detected in the tonsils of latently infect...

journal_title：Journal of clinical microbiology

authors： Perez S,Inman M,Doster A,Jones C

更新日期：2005-01-01 00:00:00

abstract：:We propose a novel application of secondary electrospray ionization-mass spectrometry (SESI-MS) as a real-time clinical diagnostic tool for bacterial infection. It is known that volatile organic compounds (VOCs), produced in different combinations and quantities by bacteria as metabolites, generate characteristic odor...

journal_title：Journal of clinical microbiology

authors： Zhu J,Bean HD,Kuo YM,Hill JE

更新日期：2010-12-01 00:00:00

abstract：:Ehrlichia chaffeensis is an obligatory intracellular bacterium of monocytes and macrophages and the etiologic agent of human monocytic ehrlichiosis, an emerging zoonosis. The Lone Star tick (Amblyomma americanum) has been implicated as the primary vector of E. chaffeensis. The present study examined the sensitivity of...

journal_title：Journal of clinical microbiology

authors： Felek S,Unver A,Stich RW,Rikihisa Y

更新日期：2001-02-01 00:00:00

abstract：:A cross-sectional study of nasopharyngeal colonization with Streptococcus pneumoniae was performed among 573 children attending 29 day-care centers (DCCs) in Norway prior to the start of mass vaccination with the heptavalent pneumococcal conjugate vaccine (PCV-7). A sensitive sampling method was employed, including tr...

journal_title：Journal of clinical microbiology

authors： Vestrheim DF,Høiby EA,Aaberge IS,Caugant DA

更新日期：2008-08-01 00:00:00

abstract：:Patients with atrophic corpus gastritis and elevated Helicobacter pylori antibody titers but 13C-urea breath test (13C-UBT) and histology results negative for H. pylori were randomized into eradication therapy or follow-up only. Antibody levels decreased significantly in six out of seven patients in the eradication gr...

journal_title：Journal of clinical microbiology

authors： Kokkola A,Rautelin H,Puolakkainen P,Sipponen P,Färkkilä M,Haapiainen R,Kosunen TU

更新日期：1998-06-01 00:00:00

abstract：:The use of counterimmunoelectrophoresis (CIE) for detection of serum antibodies to staphylococcal teichoic acids was evaluated against teichoic acids prepared by sonic treatment or lysostaphin extraction of Staphylococcus aureus (Lafferty strain). Of 54 patient sera from suspected cases of staphylococcal endocarditis,...

journal_title：Journal of clinical microbiology

authors： Leffell MS,Folds JD,Wasilauskas B

更新日期：1978-11-01 00:00:00

abstract：:Tests commonly used for routine determination of anti-Toxoplasma gondii immunoglobulin G (IgG) antibodies show a high level of consistency. However, considerable variations between commercial screening tests are still observed in detecting antibodies present at low concentrations, leading to a number of discrepant and...

journal_title：Journal of clinical microbiology

authors： Franck J,Garin YJ,Dumon H

更新日期：2008-07-01 00:00:00

abstract：:Cryptococcus gattii emerged in 1999 in a distinct ecologic environment on Vancouver Island, Canada. Estimates of incubation period were derived from an analysis of travelers with discreet exposures to this region. Individual case incubation periods ranged from 2 to 11 months, with a median of 6 to 7 months. ...

journal_title：Journal of clinical microbiology

authors： MacDougall L,Fyfe M

更新日期：2006-05-01 00:00:00

abstract：:Quantitative real-time PCR (qPCR) is increasingly being used for the detection of bovine leukemia virus (BLV) proviral DNA. Nevertheless, quality control for the validation and standardization of such tests is currently lacking. Therefore, the present study was initiated by three Office International des Epizooties (O...

journal_title：Journal of clinical microbiology

authors： Jaworski JP,Pluta A,Rola-Łuszczak M,McGowan SL,Finnegan C,Heenemann K,Carignano HA,Alvarez I,Murakami K,Willems L,Vahlenkamp TW,Trono KG,Choudhury B,Kuźmak J

更新日期：2018-06-25 00:00:00

abstract：:Our results show that impairment of the gastrointestinal tracts in human immunodeficiency virus (HIV)-positive patients is present in the early phases of HIV disease. This impairment is associated with alterations in gut microbiota and intestinal inflammatory parameters. These findings support the hypothesis that alte...

journal_title：Journal of clinical microbiology

authors： Gori A,Tincati C,Rizzardini G,Torti C,Quirino T,Haarman M,Ben Amor K,van Schaik J,Vriesema A,Knol J,Marchetti G,Welling G,Clerici M

更新日期：2008-02-01 00:00:00

abstract：:We systematically evaluated 5 methods for testing daptomycin versus 48 Enterococcus faecalis, 51 Enterococcus faecium, and 50 Staphylococcus aureus isolates using (i and ii) broth microdilution (BMD) with 50-mg/liter calcium medium supplementation (reference method) and 30-mg/liter calcium medium supplementation (BMD3...

journal_title：Journal of clinical microbiology

authors： Kirn TJ,Onyeaso E,Syed M,Weinstein MP

更新日期：2014-06-01 00:00:00

abstract：:Pasteurella species are part of the oral flora of cats and dogs. In humans, they are frequently found in infected animal bite wounds, but invasive infections are rare. This is the first report of prosthetic-valve endocarditis with a Pasteurella dagmatis-like species, which originated from the patient's cat. ...

journal_title：Journal of clinical microbiology

authors： Strahm C,Goldenberger D,Gutmann M,Kuhnert P,Graber P

更新日期：2012-08-01 00:00:00

abstract：:We compared a direct fluorescent-antibody stain (DFA) and an enzyme immunoassay (EIA) with a standard cell culture technique for the detection of Chlamydia trachomatis infection in women in an urban family practice setting. We also evaluated a DFA sample in a commercial laboratory to determine the interlaboratory reli...

journal_title：Journal of clinical microbiology

authors： Gann PH,Herrmann JE,Candib L,Hudson RW

更新日期：1990-07-01 00:00:00

abstract：:PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a rapid and convenient technique for the detection of mutations and allelic variants. We have adapted this technique for the identification of bacteria by PCR with fluorescein-labeled primers chosen from the conserved regions of the 16S rRNA gene flank...

journal_title：Journal of clinical microbiology

authors： Widjojoatmodjo MN,Fluit AC,Verhoef J

更新日期：1995-10-01 00:00:00

abstract：:The identification and elimination of persistently infected (PI) cattle are the most effective measures for controlling bovine pestiviruses, including bovine viral diarrhea virus (BVDV) and the emerging HoBi-like viruses. Here, colostrum-deprived calves persistently infected with HoBi-like pestivirus (HoBi-like PI cal...

journal_title：Journal of clinical microbiology

authors： Bauermann FV,Falkenberg SM,Vander Ley B,Decaro N,Brodersen BW,Harmon A,Hessman B,Flores EF,Ridpath JF

更新日期：2014-11-01 00:00:00

abstract：:Infections due to small-colony variants (SCVs) of Staphylococcus aureus in patients with chronic and recurrent infections are an emerging problem; however, studies with this subpopulation are hampered by the fact that SCVs may exhibit unstable phenotypes, making them difficult to study, particularly in broth media. In...

journal_title：Journal of clinical microbiology

authors： Becker K,Laham NA,Fegeler W,Proctor RA,Peters G,von Eiff C

更新日期：2006-09-01 00:00:00

abstract：:Salmonella isolates have traditionally been classified by serotyping, the serologic identification of two surface antigens, O-polysaccharide and flagellin protein. Serotyping has been of great value in understanding the epidemiology of Salmonella and investigating disease outbreaks; however, production and quality con...

journal_title：Journal of clinical microbiology

authors： McQuiston JR,Parrenas R,Ortiz-Rivera M,Gheesling L,Brenner F,Fields PI

更新日期：2004-05-01 00:00:00

abstract：:Enterobacter cloacae strain G6809 with reduced susceptibility to carbapenems was identified from a patient in a long-term acute care hospital in Kentucky. G6809 belonged to sequence type (ST) 88 and carried two carbapenemase genes, bla(KPC-18) and bla(VIM-1). Whole-genome sequencing localized bla(KPC-18) to the chromo...

journal_title：Journal of clinical microbiology

authors： Thomson GK,Snyder JW,McElheny CL,Thomson KS,Doi Y

更新日期：2016-03-01 00:00:00

abstract：:Recent studies suggest that a group of Chlamydia strains known as TWAR, which are now proposed to be a new species called Chlamydia pneumoniae, may be a frequent cause of respiratory disease in the United States and many other countries. Current serotesting methods do not allow rapid screening of large numbers of samp...

journal_title：Journal of clinical microbiology

authors： Ladany S,Black CM,Farshy CE,Ossewaarde JM,Barnes RC

更新日期：1989-12-01 00:00:00

abstract：:Using the Staphylococcus aureus absorption method, we have modified a commercial radioimmunoassay kit for the determination of total antibody to hepatitis B core antigen so that we can now determine the predominant globulin species of that antibody. One-hundred percent of acute hepatitis B sera had a predominance of i...

journal_title：Journal of clinical microbiology

authors： Schable CA,Maynard JE

更新日期：1982-11-01 00:00:00