Abstract:
:The properties of red blood cell membranes in patients with chronic renal failure were investigated using electron paramagnetic resonance spectroscopy. Using spin traps, 5,5-dimethylpirroline-1 oxide and N-tert-butyl-alpha-phenylnitrone, we found generation of hydroxyl radicals in the blood of patients with chronic renal failure after 20 min of regular hemodialysis. The physical state of membrane proteins and membrane osmotic fragility and reductive properties of red blood cells were studied. The increase in the relative correlation time of 4-(2-iodoacetamido)-2,2,6,6-tetramethylpiperidine-1 oxyl indicates the immobilization of membrane protein molecules in erythrocytes of chronic renal failure patients. The decrease in membrane protein mobility was observed in whole blood incubated with tert-butylhydroperoxide, regardless of the presence of iron. We found that the addition of ferrous ions did not aggravate profound changes in membrane proteins induced with tert-butylhydroperoxide. We also demonstrated higher osmotic fragility of erythrocytes in the patients with renal failure as compared to normal subjects. These alterations in membrane structure of red blood cells in hemodialysed patients suggest that hydroxyl radicals generated during hemodialysis can play an important role in the oxidative mechanism of erythrocyte damage.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Gwoździński K,Janicka M,Brzeszczyńska J,Luciak Msubject
Has Abstractpub_date
1997-01-01 00:00:00pages
99-107issue
1eissn
0001-527Xissn
1734-154Xjournal_volume
44pub_type
杂志文章abstract::The map of candidate genes that can potentially affect physical fitness becomes larger every year, and they are associated with such aspects as respiratory and cardiovascular stability; body build and composition - especially muscle mass and strength; carbohydrate and lipid metabolism; response to training; and exerci...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2016_1422
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abstract::beta-Secretase, a beta-site amyloid precursor protein (APP) cleaving enzyme (BACE), participates in the secretion of beta-amyloid peptides (Abeta), the major components of the toxic amyloid plaques found in the brains of patients with Alzheimer's disease (AD). According to the amyloid hypothesis, accumulation of Abeta...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
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journal_title:Acta biochimica Polonica
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doi:0350041239
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
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journal_title:Acta biochimica Polonica
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pub_type: 杂志文章
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:035001103
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journal_title:Acta biochimica Polonica
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更新日期:2011-01-01 00:00:00
abstract::The activation of NF-κB transcription factor is critical for a wide range of processes such as immunity, inflammation, cell development, growth and survival. It is activated by a variety of stimuli including cytokines, ionizing radiation and oxidative stress. Redox modulations of NF-κB pathway have been widely demonst...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2012-01-01 00:00:00
abstract::Melanins form a diverse group of pigments synthesized in living organisms in the course of hydroxylation and polymerization of organic compounds. Melanin production is observed in all large taxa from both Pro- and Eukaryota. The basic functions of melanins are still a matter of controversy and speculation, even though...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2006-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 更正并重新发布的文章,杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::In most cells, the major intracellular redox buffer is glutathione (GSH) and its disulfide-oxidized (GSSG) form. The GSH/GSSG system maintains the intracellular redox balance and the essential thiol status of proteins by thiol disulfide exchange. Topoisomerases are thiol proteins and are a target of thiol-reactive sub...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::Geranylgeranyl diphosphate (GGPP) synthase was purified to homogeneity from bovine brain in a one-step affinity column procedure. For the construction of the affinity column, a farnesyl diphosphate (FPP) analog, O-(6-amino-1-hexyl)-P-farnesylmethyl phosphonophosphate, was synthesized and linked to the spacer of the ma...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::Transcriptionally active and inactive chromatin fractions were isolated from Physarum polycephalum after depolymerization of chromatin with DNAase II or micrococcal nuclease, followed by fractionation in 5 mM-MgCl2. The active fraction of chromatin comprised up to 21% of nuclear DNA and was enriched 22-fold in the lab...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1980-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2017_2308
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journal_title:Acta biochimica Polonica
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doi:
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journal_title:Acta biochimica Polonica
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doi:
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journal_title:Acta biochimica Polonica
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:10.18388/abp.2018_2635
更新日期:2018-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2014_926
更新日期:2015-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2015_1013
更新日期:2016-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:
更新日期:1990-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2019-11-20 00:00:00