Abstract：
:This study examined the antiproliferative effects of β-escin (E) in cancer cells. The study showed that E inhibited cancer cells growth in a dose-dependent manner. The flow cytometric analysis revealed an escin-induced increase in the sub-G1 DNA content, which is considered to be a marker of apoptosis. Apoptosis was also confirmed by annexin V staining and DNA fragmentation assay. These effects were associated with increased generation of reactive oxygen species (ROS), caspase-3 activation and decreased mitochondrial membrane potential (MMP). Moreover, escin decreased mitochondrial protein content and mitochondrial fluorescence intensity as well as caused depletion of glutathione (GSH). However, activity of glutathione peroxidase (GPx) and glutathione reductase (GR) was not significantly changed in escin-treated cells. In conclusion, our results demonstrated that E has apoptotic effects in human cancer cells through the mechanisms involving mitochondrial perturbation. Although the exact mechanism needs to be investigated further, it can be concluded that E may be a useful candidate agent for cancer treatment.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Mojžišová G,Kello M,Pilátová M,Tomečková V,Vašková J,Vaško L,Bernátová S,Mirossay L,Mojžiš Jdoi
10.18388/abp.2015_1013subject
Has Abstractpub_date
2016-01-01 00:00:00pages
79-87issue
1eissn
0001-527Xissn
1734-154Xpii
1679journal_volume
63pub_type
杂志文章abstract：:Regulation of proteolytic enzyme activity is an essential requirement for cells and tissues because proteolysis at a wrong time and location may be lethal. Proteases are synthesized as inactive or less active precursor molecules in order to prevent such inappropriate proteolysis. They are activated by limited intra- o...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：035003691
更新日期：2003-01-01 00:00:00
abstract：:1. Susceptibility to inhibitors of neutral protease from calf thymus chromatin has been compared with that of trypsin. The chromatin protease reacts stoichiometrically with the inhibitors specific for trypsin (diisopropylfluorophosphate, tosyl-lysyl chloromethane, soybean trypsin inhibitor and Kunitz basic inhibitor f...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:In this study we investigated the level of 8-oxo-2'-deoxyguanosine (8-oxodG) in DNA of Cardamine pratensis plants subjected to different growth conditions trying to answer the question whether factors like light and water accessibility or low temperature may have an impact on the total DNA oxidative damage. The level ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:Pathogen-specific acquired immunity in bacteria is mediated by the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems. Thermus thermophilus strain HB8 contains CRISPR systems of several major subtypes (type I, IIIA and IIIB), and has become a widely studied model for CRISPR biology. We have...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2016_1261
更新日期：2016-01-01 00:00:00
abstract：:The approach for the unification of published proposals for the nomenclature and abbreviations of aminoalkylphosphonic acids and their derivatives is presented. Their modification was made on the basis of the IUPAC-IUB rules concerning the nomenclature and code system of proteinogenic amino acids. Our present proposal...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2014_904
更新日期：2015-01-01 00:00:00
abstract：:Lanthanides, also called rare-earth elements, are an interesting group of 15 chemically active, mainly trivalent, f-electronic, silvery-white metals. In fact, lanthanides are not as rare as the name implies, except for promethium, a radioactive artificial element not found in nature. The mean concentrations of lanthan...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2000-01-01 00:00:00
abstract：:The G protein-coupled histamine H4 receptor (H4R) is the last member of histamine receptors family discovered so far. Its expression pattern, together with postulated involvement in a wide variety of immunological and inflammatory processes make histamine H4 receptor an interesting target for drug development. Potenti...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:Selectivity of amidination using ornithine as model amino acid was investigated in detail. The results obtained were taken advantage of for studying the reactions with other polyfunctional amino acids: beta-tyrosine, isoserine, 2,3-diaminopropionic acid, 2,6-diamino-7-hydroxyazelaic acid and with the pentapeptide amid...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:A new enzymatic activity, which catalyses formation in vitro of di-O-(indole-3-acetyl)-D-glucose from 1-O-(indole-3-acetyl)-beta-D-glucose has been found in extracts of Zea mays seedlings. The structure of di-O-(indole-3-acetyl)-D-glucose, not as yet described, has been assigned by GC-MS, 1H NMR and ammonolysis. ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:Replication of lambda plasmid DNA is halted in amino acid-starved wild type (stringent) strains whereas it proceeds in relA (relaxed) mutants. The only transcription which could be important in lambda plasmid DNA replication in amino acid-starved Escherichia coli cells is that starting from the pR promoter. Using a fu...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
abstract：:Soluble alkaline phosphatase from Thiobacillus thioparus cells was purified about 230-fold. The enzyme had a mol. wt. of 50 000 daltons, optimum pH at 10.5, and was heat-resistant in the presence of diethanolamine. Polyacrylamide-gel electrophoresis demonstrated contamination of the preparation with inactive proteins ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1976-01-01 00:00:00
abstract：:1. The interaction of copper(II) with inosine, 2'-deoxyinosine, 1-methylinosine, 7-deazainosine, 6-methoxypurine riboside and IMP was examined. 2. Copper binds with the purine base of the nucleosides, and in IMP also with the phosphate group. Non interaction with ribose hydroxyl groups was observed. 3. In non-aqueous ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:Computer modelling related to the real dimensions of both the whole filament and the myosin molecule subfragments has revealed two alternative modes for myosin molecule packing which lead to the head disposition similar to that observed by EM on the surface of the cross-bridge zone of the relaxed vertebrate skeletal m...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：024904829
更新日期：2002-01-01 00:00:00
abstract：:The pools of Arg, Gly, His, Ile, Ser, Glu, Leu, Val and Asp were lower during photosynthesis at 1% O2 concentration. At the same time specific radioactivity of a number of amino acids--following 14CO2 incorporation--was different than that at atmospheric O2 concentration. This is probably due to the inhibition of the ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:To downregulate expression of the beta1 integrin subunit in endothelial cells, plasmid encoding the ribozyme cassette containing hammerhead ribozyme flanked at the 5' terminus by tRNA(Val) and at the 3' terminus by constitutive transport element sequences was constructed. When used to transfect immortalized human endo...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:(E)-2'-deoxy-2'-(fluoromethylene)-cytidine (FMdC), a deoxycytidine analog displaying a very high toxicity toward a variety of solid tumor cell lines and xenografts, is activated intracellularly by deoxycytidine kinase (dCK). We have compared cytotoxicity of FMdC towards a human promyeolocytic leukemia line HL-60 and a...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：:Two different molecular forms of bacteriolytic endo-N-acetylmuramidase (EC 3.2.1.17) were found in the Acanthamoeba castellanii culture. The enzymes were separated by column chromatography on DEAE-Sephadex or by polyacrylamide-gel electrophoresis. In young cultures only one molecular form of the enzyme was selectively...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:The comparative studies were performed on insulin receptors of erythrocytes and liver plasma membranes in fish (tench and carp) and bird (duck). The Scatchard plots indicated the presence of two pools of binding sites both in fish and duck. These pools show inter-species differences in binding ability and the number o...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Influenza virus infection is a major source of morbidity and mortality worldwide. Due to the variable effectiveness of existing vaccines, especially in the early stages of an epidemic, antiviral drugs represent the first line of defense against the virus. Currently, there are two major classes of anti-influenza drugs ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2014-01-01 00:00:00
abstract：:Leukocyte adhesion to the vascular endothelium contributes to many immunological and inflammatory disorders. These processes have been shown to be mediated by bradykinin receptor type 2 (B2R) and dopamine receptor type 2 (D2R). In a previous study, we reported the formation of a B2R-D2R heterodimer, possibly altering ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2018_2641
更新日期：2018-01-01 00:00:00
abstract：:Infectious transcripts play a key role in the research on plant viruses at the molecular level. A number of cDNA clones covering the whole genome of the Polish isolate of potato leafroll virus were constructed. Four overlapping clones were selected and assembled using restriction sites. The full copy was positioned be...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:The endothelium is a highly active organ responsible for vasculatory tone and structure, angiogenesis, as well as hemodynamic, humoral, and inflammatory responses. The endothelium is constantly exposed to blood flow, sheer stress and tension. Endothelial cells are present as a vasculature in every tissue of the body a...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2009-01-01 00:00:00
abstract：BACKGROUND:Tunneled catheters are becoming increasingly used as a permanent dialysis access. Easy way of insertion and good long-term patency make them competitive to fistulas in some groups of patients. METHODS:Late complications and survival of 180 tunneled catheters inserted from June 2010 to December 2013 in 171 u...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2015_1111
更新日期：2016-01-01 00:00:00
abstract：:This brief resume enumerates the multiple actions of melatonin as an antioxidant. This indoleamine is produced in the vertebrate pineal gland, the retina and possibly some other organs. Additionally, however, it is found in invertebrates, bacteria, unicellular organisms as well as in plants, all of which do not have a...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：0350041129
更新日期：2003-01-01 00:00:00
abstract：:Sulfate uptake, the first step of sulfate assimilation in all organisms, is a highly endoergic, ATP requiring process. It is under tight control at the transcriptional level and is additionally modulated by posttranslational modifications, which are not yet fully characterized. Sulfate anion is taken up into the cell ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2009-01-01 00:00:00
abstract：:Translation of viral proteins from subgenomic RNAs (sgRNAs) is a common strategy among positive-stranded RNA viruses. Unlike host mRNA, sgRNA of Potato leafroll virus (PLRV) does not possess a cap at its 5' end nor a poly(A) tail at the 3' terminus, both of which are known to be crucial for translation of RNA in eukar...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:In this paper transcriptional factor IIIA (TFIIIA) has been used as a probe for identity of three-dimensional-structure of eukaryotic 5S rRNAs. I was interested in finding a common motif in plant and Xenopus 5S rRNAs for TFIIIA recognition. I found that the two eukaryotic 5S rRNAs (from wheat germ and lupin seeds) are...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:The bacterial Sm-like protein Hfq affects the regulation of translation by small noncoding RNAs (sRNAs). In this way, Hfq participates in the cell adaptation to environmental stress, regulation of cellular metabolism, and bacterial virulence. The majority of known sRNAs bind complementary sequences in the 5'-untransla...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：10.18388/abp.2016_1362
更新日期：2016-01-01 00:00:00
abstract：:Adenylate deaminase (EC 3.5.4.6) has been isolated from the hearts of healthy rats and from the rats with cardionecrosis induced by administration of vitamin D in high doses. Crude extract from the necrotic hearts catalysed adenylate deamination with a higher maximum velocity and a higher apparent Km value than the ex...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
abstract：:The PCR conditions have been optimized to make the process faster and more economical. When short DNA fragments are to be amplified, the time of denaturation, annealing and extension steps can be as short as 1 s each, and the yield of PCR product is still high, sufficient for many types of analysis. The PCR can be don...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
