Regulation of beta1 integrin expression in endothelial cells by chimeric tRNA(Val) ribozyme.

Abstract:

:To downregulate expression of the beta1 integrin subunit in endothelial cells, plasmid encoding the ribozyme cassette containing hammerhead ribozyme flanked at the 5' terminus by tRNA(Val) and at the 3' terminus by constitutive transport element sequences was constructed. When used to transfect immortalized human endothelial cell line EA.hy 926, it selectively blocked the synthesis of the beta1 integrin subunit and thus inhibited migration and proliferation of the cells. Thus, this construct may be a valuable tool to control the proangiogenic phenotype of stimulated endothelial cells.

journal_name

Acta Biochim Pol

journal_title

Acta biochimica Polonica

authors

Papiewska-Pajak I,Antoszczyk S

subject

Has Abstract

pub_date

2006-01-01 00:00:00

pages

311-6

issue

2

eissn

0001-527X

issn

1734-154X

pii

20061286

journal_volume

53

pub_type

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