Abstract：
:Pathogen-specific acquired immunity in bacteria is mediated by the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems. Thermus thermophilus strain HB8 contains CRISPR systems of several major subtypes (type I, IIIA and IIIB), and has become a widely studied model for CRISPR biology. We have selected two highly expressed CRISPR spacers, crRNA 2.1 and crRNA 2.2, and have enriched endogenous T. thermophilus proteins that co-purify with these crRNAs. Mass spectroscopy indicates that the chromatography protocol enriches predominantly Csm complex subunits, but also Cmr subunits. After several chromatographic steps, size exclusion chromatography indicated a molecular mass of the crRNA associated complex of 265±69 kDa. In agreement with earlier work, crRNAs of different lengths (containing the selected spacers) were observed. Most of these were completely lost when several T. thermophilus csm genes were ablated.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Szychowska M,Siwek W,Pawolski D,Kazrani AA,Pyrc K,Bochtler Mdoi
10.18388/abp.2016_1261subject
Has Abstractpub_date
2016-01-01 00:00:00pages
377-86issue
2eissn
0001-527Xissn
1734-154Xpii
1670journal_volume
63pub_type
杂志文章abstract：:Hereditary sensory and autonomic neuropathy type 2 is a rare disorder caused by recessive mutations in the WNK1/HSN2 gene located on chromosome 12p13.33. Phenotype of the patients is characterized by severe sensory loss affecting all sensory modalities. We report a novel homozygous Lys179fsX182 (HSN2); Lys965fsX968 (W...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:Chemical modifications were used to search for catalytically important residues of rat liver arginase. The results of carbamoylation, nitration and diazotization suggest that lysyl and tyrosyl residues are not involved in the catalytic function of arginase. The modification of 5--6 tryptophanyl residues by N-bromosucc...
journal_title：Acta biochimica Polonica
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doi：
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2014_926
更新日期：2015-01-01 00:00:00
abstract：:The fuzzy oil drop model suggests that the tertiary conformation of a protein - particularly a globular one - can be likened to a spherical micelle. During the folding process, hydrophilic residues are exposed on the surface, while hydrophobic residues are retained inside the protein. The resulting hydrophobicity dist...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2019_2865
更新日期：2019-11-20 00:00:00
abstract：:To develop a more potent antithrombin agent with thrombolytic and antiplatelet properties, a new staphylokinase (SAK) variant was constructed. The kringle 2 domain (K2) of tissue type-plasminogen activator (t-PA) containing a fibrin-specific binding site (i), the RGD sequence (Arg-Gly-Asp) for the prevention of platel...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
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abstract：:Two recently synthesized groups of acridine cytostatics containing fused heterocyclic ring(s): pyrazoloacridines (PAC) and pyrazolopyrimidoacridines (PPAC) were tested in regard to their in vitro cytotoxic activity towards a panel of sensitive and resistant human tumor cell lines. The obtained results corroborate our ...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2001-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1980-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1995-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：024903703
更新日期：2002-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：045103683
更新日期：2004-01-01 00:00:00
abstract：:Enteropathogenic E. coli (EPEC) is a human pathogen using type III secretion system for delivery of proteins directly into the human host. The system contains a single ATPase, EscN, which is essential for uncoupling of proteins from their complexes with chaperones before the delivery. The structure of EscN ATPase (PDB...
journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2016_1265
更新日期：2017-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
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journal_title：Acta biochimica Polonica
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章,评审
doi：
更新日期：2006-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：035004973
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：10.18388/abp.2017_1622
更新日期：2018-01-01 00:00:00
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journal_title：Acta biochimica Polonica
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
