当前位置： SCI文献检索 > ACTA BIOCHIMICA POLONICA期刊下所有文献 > Non-histone nuclear protein HMGN2 differently regulates the urothelium barrier function by altering expression of antimicrobial peptides and tight junction protein genes in UPEC J96-infected bladder epithelial cell monolayer.

Non-histone nuclear protein HMGN2 differently regulates the urothelium barrier function by altering expression of antimicrobial peptides and tight junction protein genes in UPEC J96-infected bladder epithelial cell monolayer.

Abstract：

:The urinary tract is vulnerable to frequent challenges from environmental microflora. Uropathogenic Escherichia coli (UPEC) makes a major contribution to urinary tract infection (UTI). Previous studies have characterized positive roles of non-histone nuclear protein HMGN2 in lung epithelial innate immune response. In the study presented here, we found HMGN2 expression was up-regulated in UPEC J96-infected urothelium. Surprisingly, over-expression of HMGN2 promoted disruption of BECs 5637 cells' intercellular junctions by down-regulating tight junction (TJs) components' expression and physical structure under J96 infection. Further investigation showed that BECs 5637 monolayer, in which HMGN2 was over-expressed, had significantly increased permeability to J96. Our study systemically explored the regulatory roles of HMGN2 in BECs barrier function during UPEC infection and suggested different modulations of intracellular and paracellular routes through which UPEC invades the bladder epithelium.

journal_name

Acta Biochim Pol

journal_title

Acta biochimica Polonica

authors

Tian H,Miao J,Zhang F,Xiong F,Zhu F,Li J,Wang X,Chen S,Chen J,Huang N,Wang Y

doi

10.18388/abp.2017_1622

subject

Has Abstract

pub_date

2018-01-01 00:00:00

pages

93-100

issue

1

eissn

0001-527X

issn

1734-154X

pii

1622

journal_volume

65

pub_type

杂志文章

相关文献

ACTA BIOCHIMICA POLONICA文献大全
  • Antimicrobial, antiadhesive and antibiofilm potential of lipopeptides synthesised by Bacillus subtilis, on uropathogenic bacteria.

    abstract：:The aim of this study was to investigate the antimicrobial effect of lipopeptide biosurfactants from surfactin, iturin and fengycin families, synthesised by the Bacillus subtilis I'1a strain, on uropathogenic bacteria, including the effects on planktonic growth, processes of biofilm formation and dislodging. Antimicro...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：10.18388/abp.2015_1120

    authors： Moryl M,Spętana M,Dziubek K,Paraszkiewicz K,Różalska S,Płaza GA,Różalski A

    更新日期：2015-01-01 00:00:00

  • Up-regulation of human PNPase mRNA by beta-interferon has no effect on protein level in melanoma cell lines.

    abstract：:Human mitochondrial polynucleotide phosphorylase (hPNPase) is an exoribonuclease localized in mitochondria. The exact physiological function of this enzyme is unknown. Recent studies have revealed the existence of a relationship between induction of hPNPase mRNA and both cellular senescence and growth arrest of melano...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Gewartowski K,Tomecki R,Muchowski L,Dmochow Ska A,Dzwonek A,Malecki M,Skurzak H,Ostrowski J,Stepien PP

    更新日期：2006-01-01 00:00:00

  • Selection and analysis of a DNA aptamer binding α-amanitin from Amanita phalloides.

    abstract：:Mushroom foraging is very popular in some regions of the world. Sometimes toxic and edible mushrooms are mistaken by mushroom collectors, leading to serious human poisoning. The group of mushrooms highly dangerous for human health includes Amanita phalloides. This mushroom produces a toxic octapeptide called α-amaniti...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：10.18388/abp.2017_1615

    authors： Muszyńska K,Ostrowska D,Bartnicki F,Kowalska E,Bodaszewska-Lubaś M,Hermanowicz P,Faulstich H,Strzałka W

    更新日期：2017-01-01 00:00:00

  • New experimental and computational approaches to the analysis of gene expression.

    abstract：:Public and private EST (Expressed Sequence Tag) programs provide access to a large number of ESTs from a number of plant species, including Arabidopsis, corn, soybean, rice, wheat. In addition to the homology of each EST to genes in GenBank, information about homology to all other ESTs in the data base can be obtained...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章,评审

    doi：

    authors： Rafalski JA,Hanafey M,Miao GH,Ching A,Lee JM,Dolan M,Tingey S

    更新日期：1998-01-01 00:00:00

  • Effect of some glycolytic intermediates and palmitoyl-CoA on alpha-glycerophosphate dehydrogenase in mitochondria isolated from liver of triiodothyronine-treated rats.

    abstract：:alpha-Glycerophosphate dehydrogenase (EC 1.1.99.5) in mitochondria from liver of the triiodothyronine-treated rats is competitively inhibited by phosphoenolpyruvate, glyceraldehyde 3-phosphate and 3-phosphoglycerate, the apparent Ki values for phosphoenolpyruvate being 0.76 mM at pH 7.0, 1.7 mM at pH 7.4 and 3.5 mM at...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Swierczyński J,Scisłowski P,Aleksandrowicz Z

    更新日期：1977-01-01 00:00:00

  • Alkylated cytosine nucleosides: substrate and inhibitor properties in enzymatic deamination.

    abstract：:Cytosine nucleoside deaminase (EC 3.5.4.5) from Salmonella typhimurium LT2 catalyses the deamination of ribo-, deoxyribo- and arabinosyl nucleosides of cytosine alkylated at the C-5, but not at the N3 or exocyclic N4, of the pyrimidine ring. The enzyme was inert towards analogues etherified at the 3'-OH and 5'-OH of t...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Krajewska E,Shugar D

    更新日期：1975-01-01 00:00:00

  • Spontaneous mutagenesis in exponentially growing and stationary-phase, umuDC-proficient and -deficient, Escherichia coli dnaQ49.

    abstract：:Spontaneous mutations arise not only in exponentially growing bacteria but also in non-dividing or slowly dividing stationary-phase cells. In the latter case mutations are called adaptive or stationary-phase mutations. High spontaneous mutability has been observed in temperature sensitive Escherichia coli dnaQ49 strai...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：045103683

    authors： Nowosielska A,Nieminuszczy J,Grzesiuk E

    更新日期：2004-01-01 00:00:00

  • Gamma-radiation-induced ATM-dependent signalling in human T-lymphocyte leukemic cells, MOLT-4.

    abstract：:ATM kinase (ATM) is essential for activation of cell cycle check points and DNA repair in response to ionizing radiation (IR). In this work we studied the molecular mechanisms regulating DNA repair and cell death in human T-lymphocyte leukemic cells, MOLT-4. Apoptosis was evaluated by flow-cytometric detection of anne...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Tichý A,Záskodová D,Rezácová M,Vávrová J,Vokurková D,Pejchal J,Vilasová Z,Cerman J,Osterreicher J

    更新日期：2007-01-01 00:00:00

  • Molecular analysis of hemophilia B in Poland: 12 novel mutations of the factor IX gene.

    abstract：:We examined the molecular basis of factor IX deficiency in 53 unrelated Polish patients with hemophilia B. Heteroduplex analysis and direct sequencing of polymerase chain reaction (PCR) products were applied to identify the gene defect. Forty-three different point mutations were detected in the factor IX gene of 47 pa...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Wulff K,Bykowska K,Lopaciuk S,Herrmann FH

    更新日期：1999-01-01 00:00:00

  • Cystic fibrosis is a risk factor for celiac disease.

    abstract：BACKGROUND:The coexistence of cystic fibrosis (CF) and celiac disease (CD) has been reported. To our knowledge there is no study directly comparing the incidence of CD in CF patients to that in the general population at the same time. There is no published data on genetic predisposition to CD in CF patients either. The...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Walkowiak J,Blask-Osipa A,Lisowska A,Oralewska B,Pogorzelski A,Cichy W,Sapiejka E,Kowalska M,Korzon M,Szaflarska-Popławska A

    更新日期：2010-01-01 00:00:00

  • Congenital disorders of glycosylation. Part I. Defects of protein N-glycosylation.

    abstract：:Glycosylation is the most common chemical process of protein modification and occurs in every living cell. Disturbances of this process may be either congenital or acquired. Congenital disorders of glycosylation (CDG) are a rapidly growing disease family, with about 50 disorders reported since its first clinical descr...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章,评审

    doi：

    authors： Cylwik B,Naklicki M,Chrostek L,Gruszewska E

    更新日期：2013-01-01 00:00:00

  • X-band and S-band EPR detection of nitric oxide in murine endotoxaemia using spin trapping by ferro-di(N-(dithiocarboxy)sarcosine).

    abstract：:Ammonium salt of N-(dithiocarboxy)sarcosine (DTCS) chelated to ferrous salt was tested as an NO-metric spin trap at room temperature for ex vivo measurement of (.)NO production in murine endotoxaemia. In a chemically defined in vitro model system EPR triplet signals of NO-Fe(DTCS)(2) were observed for as long as 3 hou...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：035003799

    authors： Plonka PM,Wisniewska M,Chlopicki S,Elas M,Rosen GM

    更新日期：2003-01-01 00:00:00

  • Tyrosine aminotransferase in frog liver.

    abstract：:1. In frog liver, tyrosine aminotransferase is located mainly in cytoplasm. The enzyme is an anionic protein of mol. wt. 115 000 daltons, specific toward 2-oxoglutarate. The enzyme separates on ion-exchange chromatography into two active forms. 2. Administration of triiodotyronine in vivo induces the activity of the e...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Laskowska-Klita T

    更新日期：1975-01-01 00:00:00

  • Dendrimer-paclitaxel complexes for efficient treatment in ovarian cancer: study on OVCAR-3 and HEK293T cells.

    abstract：:The present paper investigates the enhancement of the therapeutic effect of Paclitaxel (a potent anticancer drug) by increasing its cellular uptake in the cancerous cells with subsequent reduction in its cytotoxic effects. To fulfill these goals the Paclitaxel (PTX)-Biotinylated PAMAM dendrimer complexes were prepared...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：10.18388/abp.2017_2331

    authors： Yao H,Ma J

    更新日期：2018-01-01 00:00:00

  • Immunoglobulins anti-endonuclease 32 kDa from Cucurbita pepo var patissonina affect process of DNA synthesis.

    abstract：:Immunoglobulins anti-endonuclease 32 kDa inhibit DNA synthesis. We observed that low concentrations of IgGs (about 50 micrograms IgG per 1 x 10(6) cell nuclei) temporary inhibit DNA synthesis. This inhibition concerns only the synthesis of DNA bound to the nuclear matrix (associated with isolated nuclear matrix). Prei...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Rzepecki R,Szopa J

    更新日期：1995-01-01 00:00:00

  • Functional analyses of a low-penetrance risk variant rs6702619/1p21.2 associating with colorectal cancer in Polish population.

    abstract：:Several studies employed the genome-wide association (GWA) analysis of single-nucleotide polymorphisms (SNPs) to identify susceptibility regions in colorectal cancer (CRC). However, the functional studies exploring the role of associating SNPs with cancer biology are limited. Herein, using chromatin immunoprecipitatio...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：10.18388/abp.2019_2775

    authors： Statkiewicz M,Maryan N,Kulecka M,Kuklinska U,Ostrowski J,Mikula M

    更新日期：2019-09-17 00:00:00

  • Kainate-evoked modulation of gene expression in rat brain.

    abstract：:Kainate is a glutamate analog that produces neuronal excitation resulting in seizures within hours following its intraperitoneal injection into adult rats. Then, at 2-3 days after the treatment, neurodegeneration of apoptotic character can be observed in limbic system. As a consequence, plastic reorganization and glia...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章,评审

    doi：

    authors： Kaminska B,Filipkowski RK,Biedermann IW,Konopka D,Nowicka D,Hetman M,Dabrowski M,Gorecki DC,Lukasiuk K,Szklarczyk AW,Kaczmarek L

    更新日期：1997-01-01 00:00:00

  • Conformational flexibility and ligand binding properties of ovine β-lactoglobulin.

    abstract：:Ovine β‑lactoglobulin was characterized by spectroscopic (CD), calorimetric (ITC) and X-ray structural studies. The structure of ovine β‑lactoglobulin complex with decanol showed that tight packing of molecules in the crystalline phase enforces a distortion of protein flexible loops resulting in the formation of an as...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：10.18388/abp.2019_2883

    authors： Loch J,Bonarek P,Lewiński K

    更新日期：2019-12-27 00:00:00

  • Tissue-specific effect of refeeding after short- and long-term caloric restriction on malic enzyme gene expression in rat tissues.

    abstract：:Restricting food intake to a level below that consumed voluntarily (85%, 70% and 50% of the ad libitum energy intake for 3 or 30 days) and re-feeding ad libitum for 48 h results in an increase of malic enzyme (ME) gene expression in rat white adipose tissue. The increase of ME gene expression was much more pronounced ...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：045103805

    authors： Stelmanska E,Korczynska J,Swierczynski J

    更新日期：2004-01-01 00:00:00

  • Structure of the arginase coding gene and its transcript in Aspergillus nidulans.

    abstract：:The arginase structural gene (agaA) from Aspergillus nidulans has been cloned and characterised. Depending on the growth conditions of the mycelium, transcripts of this gene have different 5'ends. These differences could result either from the presence of multiple transcription initiation sites or from differential pr...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Borsuk P,Dzikowska A,Empel J,Grzelak A,Grześkowiak R,Weglenski P

    更新日期：1999-01-01 00:00:00

  • Cellular level of 8-oxo-2'-deoxyguanosine in DNA does not correlate with urinary excretion of the modified base/nucleoside.

    abstract：:We assessed a relationship between the level of 8-oxodG in leukocyte DNA measured with the high performance liquid chromatography with electrochemical detection (HPLC/EC) technique and urinary excretion of the modified nucleoside/base analysed with a recently developed methodology involving HPLC prepurification follow...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：035002549

    authors： Foksinski M,Gackowski D,Rozalski R,Olinski R

    更新日期：2003-01-01 00:00:00

  • Prediction of the structure of the common perimitochondrial localization signal of nuclear transcripts in yeast.

    abstract：:Many nuclear genes encoding mitochondrial proteins require specific localization of their mRNAs to the vicinity of mitochondria for proper expression. Studies in Saccharomyces cerevisiae have shown that the cis-acting signal responsible for subcellular localization of mRNAs is localized in the 3' UTR of the transcript...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Ejsmont RK,Golik P,Stepien PP

    更新日期：2007-01-01 00:00:00

  • Evaluation of high temperature glycation of proteins and peptides by electrospray ionization mass spectrometry.

    abstract：:Recently Boratyński & Roy (Glycoconjugate J., 1998, 15, 131) described a fast and convenient procedure for the synthesis of glycoconjugates. In the present study we used ESI-MS and circular dichroism as tools to analyze non-enzymatic glycation products of proteins and peptides. We discuss influence of reaction conditi...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Stefanowicz P,Boratyński J,Kańska U,Petry I,Szewczuk Z

    更新日期：2001-01-01 00:00:00

  • Characterization of a novel transcript variant of human STAU1 gene.

    abstract：:Human STAU1 is one member of the family of double-stranded RNA (dsRNA)-binding proteins. It is thought to function in transporting mRNA, controlling translation and eliciting mRNA decay in neurons, and to function in infection of influenza virus and human immunodeficiency virus type 1 (HIV-1). Four transcripts coding ...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Fa-Hui N,Hai-Feng Y,Rui-Feng Q,Xin L,Cai-Bin W

    更新日期：2008-01-01 00:00:00

  • Advanced oxidation protein products and inflammatory markers in liver cirrhosis: a comparison between alcohol-related and HCV-related cirrhosis.

    abstract：:Advanced oxidation protein products (AOPPs) are protein markers of oxidative stress with pro-inflammatory properties that accumulated in liver cirrhosis. In the present study, we investigated the association between chronic inflammatory response triggered by AOPPs and the severity of liver disease as assessed by the C...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Zuwała-Jagiełło J,Pazgan-Simon M,Simon K,Warwas M

    更新日期：2011-01-01 00:00:00

  • The nomenclature of 1-aminoalkylphosphonic acids and derivatives: evolution of the code system.

    abstract：:The approach for the unification of published proposals for the nomenclature and abbreviations of aminoalkylphosphonic acids and their derivatives is presented. Their modification was made on the basis of the IUPAC-IUB rules concerning the nomenclature and code system of proteinogenic amino acids. Our present proposal...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：10.18388/abp.2014_904

    authors： Drabowicz J,Jakubowski H,Kudzin MH,Kudzin ZH

    更新日期：2015-01-01 00:00:00

  • A novel homozygous mutation in the WNK1/HSN2 gene causing hereditary sensory neuropathy type 2.

    abstract：:Hereditary sensory and autonomic neuropathy type 2 is a rare disorder caused by recessive mutations in the WNK1/HSN2 gene located on chromosome 12p13.33. Phenotype of the patients is characterized by severe sensory loss affecting all sensory modalities. We report a novel homozygous Lys179fsX182 (HSN2); Lys965fsX968 (W...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Potulska-Chromik A,Kabzińska D,Lipowska M,Kostera-Pruszczyk A,Kochański A

    更新日期：2012-01-01 00:00:00

  • Reactions of Pseudomonas aeruginosa pyocyanin with reduced glutathione.

    abstract：:Pseudomonas aeruginosa is the most common cause of chronic and recurrent lung infections in patients with cystic fibrosis (CF) whose sputa contain copious quantities of P. aeruginosa toxin, pyocyanin. Pyocyanin triggers tissue damage mainly by its redox cycling and induction of reactive oxygen species (ROS). The react...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Cheluvappa R,Shimmon R,Dawson M,Hilmer SN,Le Couteur DG

    更新日期：2008-01-01 00:00:00

  • Comparative analysis of biochemical properties of mesophyll and bundle sheath chloroplasts from various subtypes of C4 plants grown at moderate irradiance.

    abstract：:The photochemical characteristics of mesophyll and bundle sheath chloroplasts isolated from the leaves of C4 species were investigated in Zea mays (NADP-ME type), Panicum miliaceum (NAD-ME type) and Panicum maximum (PEP-CK type) plants. The aim of this work was to gain information about selected photochemical properti...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章

    doi：

    authors： Romanowska E,Drozak A

    更新日期：2006-01-01 00:00:00

  • Conventional calpains and programmed cell death.

    abstract：:The evidence on the crucial role of a family of calcium-dependent cysteine proteases called calpains in programmed cell death is rich and still growing. However, understanding of the mechanisms of their functions in apoptosis is not full yet. Calpains have been implicated in both physiological and pathological cell de...

    journal_title：Acta biochimica Polonica

    pub_type： 杂志文章,评审

    doi：

    authors： Łopatniuk P,Witkowski JM

    更新日期：2011-01-01 00:00:00