Abstract:
:The present paper investigates the enhancement of the therapeutic effect of Paclitaxel (a potent anticancer drug) by increasing its cellular uptake in the cancerous cells with subsequent reduction in its cytotoxic effects. To fulfill these goals the Paclitaxel (PTX)-Biotinylated PAMAM dendrimer complexes were prepared using biotinylation method. The primary parameter of Biotinylated PAMAM with a terminal HN2 group - the degree of biotinylation - was evaluated using HABA assay. The basic integrity of the complex was studied using DSC. The Drug Loading (DL) and Drug Release (DR) parameters of Biotinylated PAMAM dendrimer-PTX complexes were also examined. Cellular uptake study was performed in OVCAR-3 and HEK293T cells using fluorescence technique. The statistical analysis was also performed to support the experimental data. The results obtained from HABA assay showed the complete biotinylation of PAMAM dendrimer. DSC study confirmed the integrity of the complex as compared with pure drug, biotinylated complex and their physical mixture. Batch 9 showed the highest DL (12.09%) and DR (70%) for 72 h as compared to different concentrations of drug and biotinylated complex. The OVCAR-3 (cancerous) cells were characterized by more intensive cellular uptake of the complexes than HEK293T (normal) cells. The obtained experimental results were supported by the statistical data. The results obtained from both experimental and statistical evaluation confirmed that the biotinylated PAMAM NH2 dendrimer-PTX complex not only displays increased cellular uptake but has also enhanced release up to 72 h with the reduction in cytotoxicity.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Yao H,Ma Jdoi
10.18388/abp.2017_2331subject
Has Abstractpub_date
2018-01-01 00:00:00pages
219-225issue
2eissn
0001-527Xissn
1734-154Xpii
2331journal_volume
65pub_type
杂志文章abstract::Restricting food intake to a level below that consumed voluntarily (85%, 70% and 50% of the ad libitum energy intake for 3 or 30 days) and re-feeding ad libitum for 48 h results in an increase of malic enzyme (ME) gene expression in rat white adipose tissue. The increase of ME gene expression was much more pronounced ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:045103805
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doi:
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pub_type: 杂志文章
doi:10.18388/abp.2019_2775
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journal_title:Acta biochimica Polonica
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journal_title:Acta biochimica Polonica
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doi:055201109
更新日期:2005-01-01 00:00:00
abstract::Diabetes is one of the major challenges of modern medicine, as it is considered a global epidemic of the XXI century. The disease often leads to the development of serious, health threatening complications. Diabetic foot syndrome is a characteristic set of anatomical and molecular changes. At the macroscopic level, ma...
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pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2016_1481
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:10.18388/abp.2018_2635
更新日期:2018-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
abstract::We investigated the effect of the cyclic AMP-protein kinase A (PKA) signalling pathway on renal Na(+),K(+)-ATPase and ouabain-sensitive H(+),K(+)-ATPase. Male Wistar rats were anaesthetized and catheter was inserted through the femoral artery into the abdominal aorta proximally to the renal arteries for infusion of th...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:035001103
更新日期:2003-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2006-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:1997-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Aspergillus fumigatus is one of the most prevalent airborne fungal pathogens causing infections worldwide. Most A. fumigatus strains are susceptible to azoles, which are administered as the first line therapeutics. However, during last decade the acquired resistance to triazoles by these species has been described. Th...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2015_1143
更新日期:2015-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The aim of this study was to analyse the correlation between HPV16 E6 variants and the physical status of viral genome (integrated, mixed, episomal) among patients with cervical cancer (n=40) and low-grade squamous intraepithelial lesions - LSIL (n=40). The study was performed on 80 HPV16 positive samples. HPV16 E6 va...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2016_1364
更新日期:2017-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:
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doi:
更新日期:2013-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:0350041239
更新日期:2003-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:
更新日期:1997-01-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.18388/abp.2015_1106
更新日期:2016-01-01 00:00:00
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doi:
更新日期:2014-01-01 00:00:00
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doi:
更新日期:2014-01-01 00:00:00
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doi:
更新日期:1980-01-01 00:00:00
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pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00