Abstract：
:Mushroom foraging is very popular in some regions of the world. Sometimes toxic and edible mushrooms are mistaken by mushroom collectors, leading to serious human poisoning. The group of mushrooms highly dangerous for human health includes Amanita phalloides. This mushroom produces a toxic octapeptide called α-amanitin which is an inhibitor of nuclear RNA polymerase II. The inhibition of this polymerase results in the abortion of mRNA synthesis. The ingestion of A. phalloides causes liver failure due to the fact that most of the toxin is uptaken by hepatocytes. The hospitalization of poisoned patients involves the removal of the toxin from the digestive tract, its dilution in the circulatory system and the administration of therapeutic adjuvants. Since there is no effective antidote against amanitin poisoning, in this study we developed a DNA aptamer exhibiting specific binding to α-amanitin. This aptamer was selected using the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) method. Next, its ability of toxin removal from aqueous solution was confirmed by pull-down assay. The aptamer region sufficient for α-amanitin binding was determined. Finally, the dissociation constant of the α-amanitin/DNA aptamer complex was calculated.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Muszyńska K,Ostrowska D,Bartnicki F,Kowalska E,Bodaszewska-Lubaś M,Hermanowicz P,Faulstich H,Strzałka Wdoi
10.18388/abp.2017_1615subject
Has Abstractpub_date
2017-01-01 00:00:00pages
401-406issue
3eissn
0001-527Xissn
1734-154Xpii
1615journal_volume
64pub_type
杂志文章abstract：:In the haemolymph of Oniscidea haemocyanin exists mainly in the form of hexamer 16S (90%); the 5S and 24S components are present in small amounts. Alkaline dissociation of the examined 16S haemocyanins is dependent on pH and divalent cations (Ca2+,Mg2+). The 16S component consists of 23-24.5% acidic amino acids, 15-18...
journal_title：Acta biochimica Polonica
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doi：
更新日期：1995-01-01 00:00:00
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doi：
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doi：
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更新日期：2007-01-01 00:00:00
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doi：
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更新日期：1998-01-01 00:00:00
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doi：
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更新日期：1980-01-01 00:00:00
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pub_type： 杂志文章,评审
doi：
更新日期：2006-01-01 00:00:00
