Abstract:
:The fucosyltransferase NodZ is involved in the biosynthesis of the nodulation factor in nitrogen-fixing symbiotic bacteria. It catalyzes alpha1,6 transfer of l-fucose from GDP-fucose to the reducing residue of the synthesized Nod oligosaccharide. We present the structure of the NodZ protein from Bradyrhizobium expressed in Escherichia coli and crystallized in the presence of phosphate ions in two crystal forms. The enzyme is arranged into two domains of nearly equal size. Although NodZ falls in one broad class (GT-B) with other two-domain glycosyltransferases, the topology of its domains deviates from the canonical Rossmann fold, with particularly high distortions in the N-terminal domain. Mutational data combined with structural and sequence alignments indicate residues of potential importance in GDP-fucose binding or in the catalytic mechanism. They are all clustered in three conserved sequence motifs located in the C-terminal domain.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Brzezinski K,Stepkowski T,Panjikar S,Bujacz G,Jaskolski Msubject
Has Abstractpub_date
2007-01-01 00:00:00pages
537-49issue
3eissn
0001-527Xissn
1734-154Xpii
20071502journal_volume
54pub_type
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journal_title:Acta biochimica Polonica
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journal_title:Acta biochimica Polonica
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