Cellular level of 8-oxo-2'-deoxyguanosine in DNA does not correlate with urinary excretion of the modified base/nucleoside.

abstract：:Geranylgeranyl diphosphate (GGPP) synthase was purified to homogeneity from bovine brain in a one-step affinity column procedure. For the construction of the affinity column, a farnesyl diphosphate (FPP) analog, O-(6-amino-1-hexyl)-P-farnesylmethyl phosphonophosphate, was synthesized and linked to the spacer of the ma...

journal_title：Acta biochimica Polonica

authors： Sagami H,Morita Y,Korenaga T,Ogura K

更新日期：1994-01-01 00:00:00

abstract：:The influence of exogenous inorganic pyrophosphate on the transport of oleanolic acid monoglucoside and monoglucuronide to vacuoles isolated from Calendula officinalis leaf protoplasts was studied. The results indicate that the transport of both monoglycosides is carrier-mediated; however, the transport of the monoglu...

journal_title：Acta biochimica Polonica

authors： Szakiel A,Janiszowska W

更新日期：1998-01-01 00:00:00

abstract：:The proteolytic activity of three Pseudomonas aeruginosa strains, ATCC 27853 - a reference strain, and two clinical isolates was tested. The activity was examined after culturing the bacteria in two different growth media: the minimal M9 medium and rich Luria-Bertani broth (LB). Based on zymograms and protease activit...

journal_title：Acta biochimica Polonica

authors： Andrejko M,Zdybicka-Barabas A,Janczarek M,Cytryńska M

更新日期：2013-01-01 00:00:00

abstract：:Gene promoters contain several classes of functional sequence elements (cis elements) recognized by protein agents, e.g. transcription factors and essential components of the transcription machinery. Here we describe a common DNA regulatory element (tandem TCTCGCGAGA motif) of human TATA-less promoters. A combination ...

journal_title：Acta biochimica Polonica

authors： Wyrwicz LS,Gaj P,Hoffmann M,Rychlewski L,Ostrowski J

更新日期：2007-01-01 00:00:00

abstract：:This study aimed to evaluate the antidotal potency of tenocyclidine (TCP) that probably might protect acetylcholinesterase (AChE) in the case of organophosphate poisoning. TCP was tested alone as a pretreatment or in combination with atropine as a therapy in rats poisoned with (1/4) and (1/2) of LD(50) of soman. Possi...

journal_title：Acta biochimica Polonica

authors： Petek MJ,Berend S,Kopjar N,Zeljezić D,Mladinić M,Radić B,Vrdoljak AL

更新日期：2008-01-01 00:00:00

abstract：:The bacterial Sm-like protein Hfq affects the regulation of translation by small noncoding RNAs (sRNAs). In this way, Hfq participates in the cell adaptation to environmental stress, regulation of cellular metabolism, and bacterial virulence. The majority of known sRNAs bind complementary sequences in the 5'-untransla...

journal_title：Acta biochimica Polonica

authors： Wroblewska Z,Olejniczak M

更新日期：2016-01-01 00:00:00

abstract：:Standard ocular tumor treatment includes brachytherapy, as well as proton therapy, particularly for large melanoma tumors. However, the effects of different radiation types on the metastatic spread is not clear. We aimed at comparing ruthenium ((106)Ru, emitting β electrons) and iodine ((125)I, γ-radiation) brachyther...

journal_title：Acta biochimica Polonica

authors： Romanowska-Dixon B,Elas M,Swakoń J,Sowa U,Ptaszkiewicz M,Szczygieł M,Krzykawska M,Olko P,Urbańska K

更新日期：2013-01-01 00:00:00

abstract：:1. The activity of trypsin inhibitors in lung and pancreas increased with age of the animals. 2. In foetal lung the activity of the basic Kunitz-type inhibitor was 1% that, and in calf lung 5-30% that, found in adult animals. 3. In foetal pancreas the basic Kunitz-type inhibitor was not detected, and in calf pancreas ...

journal_title：Acta biochimica Polonica

authors： Wilusz T,Wilimowska-Pelc A,Mejbaum-Katzenellenbogen W

更新日期：1976-01-01 00:00:00

abstract：:Many nuclear genes encoding mitochondrial proteins require specific localization of their mRNAs to the vicinity of mitochondria for proper expression. Studies in Saccharomyces cerevisiae have shown that the cis-acting signal responsible for subcellular localization of mRNAs is localized in the 3' UTR of the transcript...

journal_title：Acta biochimica Polonica

authors： Ejsmont RK,Golik P,Stepien PP

更新日期：2007-01-01 00:00:00

abstract：:The aim of this study was to evaluate the therapeutic potential of oxidative stress (OS) reduction by using pyridoindole (PI) antioxidants in adjuvant arthritis (AA). The substances tested were stobadine dipalmitate (STB) and SMe1. AA was used as animal model. The experiments included healthy animals, control arthriti...

journal_title：Acta biochimica Polonica

authors： Ponist S,Mihalova D,Jancinova V,Snirc V,Ondrejickova O,Mascia C,Poli G,Stancikova M,Nosal R,Bauerová K

更新日期：2010-01-01 00:00:00

abstract：:The purpose of this study was to examine the effects of oxidative stress caused by hydroperoxide (H(2)O(2)) in the presence of iron ions (Fe(2+)) on mitochondria of the amoeba Acanthamoeba castellanii. We used isolated mitochondria of A. castellanii and exposed them to four levels of H(2)O(2) concentration: 0.5, 5, 15...

journal_title：Acta biochimica Polonica

authors： Trocha LK,Stobienia O

更新日期：2007-01-01 00:00:00

abstract：:Nasopharyngeal carcinoma (NPC) cells are highly radiosensitive. Historically, the main modality for NPC has been radiotherapy. Now, to explore a novel and more effective approach to NPC therapy, a combined strategy of suicide gene and radiation was developed. The yeast CDglyTK (yCDglyTK) gene controlled by a synthetic...

journal_title：Acta biochimica Polonica

authors： Tang A,Zhang J,Tang Y,Feng Y,Wu L,Pan Q,Long Z,Dai H,Zhao S,Liu T,Chen Z,Xia K,Xia J

更新日期：2005-11-06 00:00:00

abstract：:The photochemical characteristics of mesophyll and bundle sheath chloroplasts isolated from the leaves of C4 species were investigated in Zea mays (NADP-ME type), Panicum miliaceum (NAD-ME type) and Panicum maximum (PEP-CK type) plants. The aim of this work was to gain information about selected photochemical properti...

journal_title：Acta biochimica Polonica

authors： Romanowska E,Drozak A

更新日期：2006-01-01 00:00:00

abstract：:Recently Boratyński & Roy (Glycoconjugate J., 1998, 15, 131) described a fast and convenient procedure for the synthesis of glycoconjugates. In the present study we used ESI-MS and circular dichroism as tools to analyze non-enzymatic glycation products of proteins and peptides. We discuss influence of reaction conditi...

journal_title：Acta biochimica Polonica

authors： Stefanowicz P,Boratyński J,Kańska U,Petry I,Szewczuk Z

更新日期：2001-01-01 00:00:00

abstract：:Annexins belong to a family of multi-functional membrane- and Ca(2+)-binding proteins. The characteristic feature of these proteins is that they can bind membrane phospholipids in a reversible, Ca(2+)-dependent manner. While animal annexins have been known for a long time and are fairly well characterized, their plant...

journal_title：Acta biochimica Polonica

authors： Talukdar T,Gorecka KM,de Carvalho-Niebel F,Downie JA,Cullimore J,Pikula S

更新日期：2009-01-01 00:00:00

abstract：:The map of candidate genes that can potentially affect physical fitness becomes larger every year, and they are associated with such aspects as respiratory and cardiovascular stability; body build and composition - especially muscle mass and strength; carbohydrate and lipid metabolism; response to training; and exerci...

journal_title：Acta biochimica Polonica

authors： Karpowicz K,Krych K,Karpowicz M,Nowak W,Gronek P

更新日期：2018-01-01 00:00:00

abstract：:The fuzzy oil drop model suggests that the tertiary conformation of a protein - particularly a globular one - can be likened to a spherical micelle. During the folding process, hydrophilic residues are exposed on the surface, while hydrophobic residues are retained inside the protein. The resulting hydrophobicity dist...

journal_title：Acta biochimica Polonica

authors： Ptak-Kaczor M,Banach M,Konieczny L,Roterman I

更新日期：2019-11-20 00:00:00

abstract：:The reaction of nitrite (NO2-) with horseradish peroxidase and lactoperoxidase was studied. Sequential mixing stopped-flow measurements gave the following values for the rate constants of the reaction of nitrite with compounds II (oxoferryl heme intermediates) of horseradish peroxidase and lactoperoxidase at pH 7.0, 1...

journal_title：Acta biochimica Polonica

authors： Gebicka L

更新日期：1999-01-01 00:00:00

abstract：:Recombination of subunits of rat liver arginase A1 and rat kidney arginase A4 yielded a product which in polyacrylamide gel electrophoresis and DEAE-cellulose chromatography separated into five proteins with arginase activity. Proteins I and V corresponded in polyacrylamide gel-electrophoresis, DEAE-cellulose chromato...

journal_title：Acta biochimica Polonica

authors： Skrzypek-Osiecka I,Porembska Z

更新日期：1983-01-01 00:00:00

abstract：:Several ribonucleoside analogues with modifications in the nucleobase and sugar moiety have been screened for anti-glioma activity in the T98G glioma cell line using cervical (HeLa) cell line as reference human malignant cells, and lung fibroblast (MCR-5) cell line as non-cancerous reference cells. Among the investiga...

journal_title：Acta biochimica Polonica

authors： Framski G,Wawrzyniak D,Jahnz-Wechmann Z,Szymanska-Michalak A,Kraszewski A,Barciszewski J,Boryski J,Stawinski J

更新日期：2016-01-01 00:00:00

abstract：:(E)-2'-deoxy-2'-(fluoromethylene)-cytidine (FMdC), a deoxycytidine analog displaying a very high toxicity toward a variety of solid tumor cell lines and xenografts, is activated intracellularly by deoxycytidine kinase (dCK). We have compared cytotoxicity of FMdC towards a human promyeolocytic leukemia line HL-60 and a...

journal_title：Acta biochimica Polonica

authors： Grie P,Koronkiewicz M,Skierski JS

更新日期：2000-01-01 00:00:00

abstract：:The endogenous electric field may provide an important signal for directional cell migration during cancer metastasis but the mechanism of cell electrotaxis is poorly understood. It was postulated that microtubules play a central role in the polarization and directional migration of several types of cells. In this pap...

journal_title：Acta biochimica Polonica

authors： Krecioch I,Madeja Z,Lasota S,Zimolag E,Sroka J

更新日期：2015-01-01 00:00:00

abstract：:Betapapillomaviruses have been linked to the development of nonmelanoma skin cancers. A great diversity of these viruses in skin specimens requires the use of sensitive and reliable detection methods. There are currently no standardized assays for diagnostic purposes. A combination of several molecular methods has gre...

journal_title：Acta biochimica Polonica

authors： Kopeć J,Szostek S,Sułowicz J,Zawilińska B

更新日期：2020-06-08 00:00:00

abstract：:A cDNA clone encoding an amphioxus fatty acid binding protein-like (AmphiFABPL) protein was isolated from a gut cDNA library of Branchiostoma belcheri. It contained a 423 bp open reading frame corresponding to a deduced protein of 140 amino acids with a predicted molecular mass of approximately 15.9 kDa. Phylogenetic ...

journal_title：Acta biochimica Polonica

authors： Wang Y,Zhang Y,Zhang S,Tian J,Jiang S

更新日期：2008-01-01 00:00:00

abstract：:Changes in the composition of fatty acids (FA) were determined in lipid extracts isolated from developing ovaries containing ovules and developing seeds of Echium vulgare L. The samples were collected successively over 20 days beginning with the first day after flowering. The contents of the n-6 FA family members, i.e...

journal_title：Acta biochimica Polonica

authors： Stolyhwo A,Mol J

更新日期：2007-01-01 00:00:00

abstract：:Liposomes made of sphingomyelin were found to inhibit both ATP-activated and non-activated AMP deaminase from pig brain, in contrast to liposomes made of egg yolk phosphatidylcholine which exhibited an activating effect on the ATP-activated enzyme, being without effect on AMP deaminase in the absence of ATP. Dioleoylp...

journal_title：Acta biochimica Polonica

authors： Woźniak M,Purzycka-Preis J,Kossowska E,Zydowo MM

更新日期：1987-01-01 00:00:00

abstract：:This brief resume enumerates the multiple actions of melatonin as an antioxidant. This indoleamine is produced in the vertebrate pineal gland, the retina and possibly some other organs. Additionally, however, it is found in invertebrates, bacteria, unicellular organisms as well as in plants, all of which do not have a...

journal_title：Acta biochimica Polonica

authors： Reiter RJ,Tan DX,Mayo JC,Sainz RM,Leon J,Czarnocki Z

更新日期：2003-01-01 00:00:00

abstract：:Cyclosporine A (CsA), a widely used immunosuppressive drug, exerts nephrotoxic activities, as demonstrated by increased tubulointerstitial fibrosis, inflammation and podocyte damage. Recently, a number of microRNAs expressed in the kidney have been reported to be elevated during renal damage. Our aim was to investigat...

journal_title：Acta biochimica Polonica

authors： Łoboda A,Mucha O,Podkalicka P,Sobczak M,Miksza-Cybulska A,Kaczara P,Jozkowicz A,Dulak J

更新日期：2018-11-27 00:00:00

abstract：:Secondary structure of alpha (light) and beta (heavy) chains of human haptoglobin was proposed. The Chou-Fasman predictive method was used in conjunction with the hydrophobic character and hydropathy scale value assigned to each amino acid residue in the primary sequence of the protein. Moreover, by means of hydrophil...

journal_title：Acta biochimica Polonica

authors： Dobryszycka W,Kubisa W,Kubicz Z

更新日期：1987-01-01 00:00:00

abstract：:Trans-diamminedichloroplatinum(II) (trans-DDP) forms with DNA at 37 degrees C, more numerous interstrand cross-links than cis-DDP in the isolated DNA and DNA in the chromatin complex. An increase in the temperature to 42.5 degrees C had no effect on the interstrand cross-links of DND-Pt-DNA formed by the two isomers, ...

journal_title：Acta biochimica Polonica

authors： Kulamowicz I,Walter Z,Krupa R

更新日期：1990-01-01 00:00:00