Abstract:
:We found that it is possible to use eriochrome black T as a dye for agarose gel electrophoresis of DNA. The presence of the dye does not change the migration rate of DNA and does not influence the electrophoretical picture.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Obuchowski M,Wegrzyn Gsubject
Has Abstractpub_date
1991-01-01 00:00:00pages
177-9issue
1eissn
0001-527Xissn
1734-154Xjournal_volume
38pub_type
杂志文章abstract::Nasopharyngeal carcinoma (NPC) cells are highly radiosensitive. Historically, the main modality for NPC has been radiotherapy. Now, to explore a novel and more effective approach to NPC therapy, a combined strategy of suicide gene and radiation was developed. The yeast CDglyTK (yCDglyTK) gene controlled by a synthetic...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2005-11-06 00:00:00
abstract::The photochemical characteristics of mesophyll and bundle sheath chloroplasts isolated from the leaves of C4 species were investigated in Zea mays (NADP-ME type), Panicum miliaceum (NAD-ME type) and Panicum maximum (PEP-CK type) plants. The aim of this work was to gain information about selected photochemical properti...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::In the post-mitochondrial fraction of murine LBN/b leukemic cells, four fractions with DNA polymerase activity (I, II, III, IV) were found. On the basis of ion exchanger affinity and poly(A), poly(C) and poly(Cm) replication ability, fraction I was classified as RNA-directed DNA polymerase of viral origin. On the basi...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1976-01-01 00:00:00
abstract::This study examined the antiproliferative effects of β-escin (E) in cancer cells. The study showed that E inhibited cancer cells growth in a dose-dependent manner. The flow cytometric analysis revealed an escin-induced increase in the sub-G1 DNA content, which is considered to be a marker of apoptosis. Apoptosis was a...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2015_1013
更新日期:2016-01-01 00:00:00
abstract::Geranylgeranyl diphosphate (GGPP) synthase was purified to homogeneity from bovine brain in a one-step affinity column procedure. For the construction of the affinity column, a farnesyl diphosphate (FPP) analog, O-(6-amino-1-hexyl)-P-farnesylmethyl phosphonophosphate, was synthesized and linked to the spacer of the ma...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract::In the present work, we have reviewed data showing that triiodothyronine and its nuclear receptors modify expression of different genes/proteins involved in cell cycle control beginning from growth factors (such as EGF and TGF-beta), to cell surface receptors (EGFR), as well as proteins acting at the cell membrane (Ra...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2006-01-01 00:00:00
abstract::Triterpene saponosides are widely distributed plant secondary metabolites characterized by relatively low systemic cytotoxicity and a range of biological activities. These include anti-inflammatory, antimicrobial, vasoprotective and antitumor properties. In particular, the ability of saponins to enhance the cytotoxici...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::So far, azide has been consistently reported to act as an inhibitor of metal enzymes, especially copper proteins. The present work shows that azide can also act as an activator of polyphenol oxidase II (PPO II) from tobacco leaves. From the square-wave voltammetry of native PPO II, peroxide-PPO II complex and azide-PP...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:0249041029
更新日期:2002-01-01 00:00:00
abstract::Inhibition of jack bean activity by 2,5-dichloro-1,4-benzoquinone (DCBQ) was studied in phosphate buffer, pH 7.0. It was found that DCBQ acted as a strong, time and concentration dependent inactivator of urease. Under the experimental conditions obeyed the terms of pseudo-first-order reaction, urease was totally inact...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::Epidemiological studies have revealed a strong correlation between high intake of fruit and vegetables and low incidence of certain cancers. Micronutrients present in these foods are thought to decrease free radical attack on DNA and hence protect against mutations that cause cancer, but the fine details of the causal...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Computer modelling related to the real dimensions of both the whole filament and the myosin molecule subfragments has revealed two alternative modes for myosin molecule packing which lead to the head disposition similar to that observed by EM on the surface of the cross-bridge zone of the relaxed vertebrate skeletal m...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:024904829
更新日期:2002-01-01 00:00:00
abstract::Melanins form a diverse group of pigments synthesized in living organisms in the course of hydroxylation and polymerization of organic compounds. Melanin production is observed in all large taxa from both Pro- and Eukaryota. The basic functions of melanins are still a matter of controversy and speculation, even though...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2006-01-01 00:00:00
abstract::An analysis has been made, with the aid of 1H NMR spectroscopy, of the solution conformation of the known antimetabolite, 9-beta-D-xylofuranosyladenine (xyloA), and of its 8-bromo analogue. For xyloA, the results point to a strong preference for the sugar ring of the conformation type N (C(3') endo), a relatively low ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1979-01-01 00:00:00
abstract::In this study we determined Vi bacteriophage III sensitivity to native cisplatin, gamma radiation ((60)Co) or to irradiated cisplatin, and checked the possibility of enhanced Vi bacteriophage III inactivation under combined exposure to cisplatin and gamma radiation. We used highly purified phage suspensions in 0.9% Na...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::The fuzzy oil drop model suggests that the tertiary conformation of a protein - particularly a globular one - can be likened to a spherical micelle. During the folding process, hydrophilic residues are exposed on the surface, while hydrophobic residues are retained inside the protein. The resulting hydrophobicity dist...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2019_2865
更新日期:2019-11-20 00:00:00
abstract::Angioarrestin is a recently described anti-angiogenic protein whose expression is down-regulated in solid tumours of various origins. It has a sequence identical to angiopoietin related protein-1. In this study we investigated anti-tumour properties of angioarrestin in B16 (F10) melanoma tumour model. We constructed a...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::Progressive decline in fertility and sperm quality has been reported over the last few decades, especially in industrialized nations. It has been proposed that exposure to factors that induce damage in DNA of spermatogenic cells may significantly contribute to impaired fertility. Here, the (32)P-postlabelling method w...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:035001197
更新日期:2003-01-01 00:00:00
abstract::In most cells, the major intracellular redox buffer is glutathione (GSH) and its disulfide-oxidized (GSSG) form. The GSH/GSSG system maintains the intracellular redox balance and the essential thiol status of proteins by thiol disulfide exchange. Topoisomerases are thiol proteins and are a target of thiol-reactive sub...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::The protective action of quercetin against the pro-oxidant and apoptotic effect of etoposide was investigated in HL-60 cells with a high level of myeloperoxidase (MPO) activity and in cells treated with MPO inhibitor, 4-aminobenzoic acid hydrazide (ABAH). Quercetin significantly protected MPO-rich cells against the pr...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::Glutathione-S-transferase (GST) a Phase-II drug detoxification enzyme, was detected in Setaria cervi, a bovine filarial parasite. In vitro effect of diethylcarbamazine, butylated hydroxyanisole and phenobarbitone on the GST of adult female S. cervi was assayed by the addition of these compounds in the maintenance medi...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract:INTRODUCTION:Hepatitis C virus (HCV) infection is a global health problem which can lead to liver cirrhosis or hepatocellular carcinoma in one-fifth of chronically infected patients. MATERIALS AND METHODS:The study group consisted of 123 patients: 90 with HCV mono- and 33 with HIV/HCV co-infection, who were treated wi...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract::We examined the molecular basis of factor IX deficiency in 53 unrelated Polish patients with hemophilia B. Heteroduplex analysis and direct sequencing of polymerase chain reaction (PCR) products were applied to identify the gene defect. Forty-three different point mutations were detected in the factor IX gene of 47 pa...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Genotoxic activities of flavonoids (quercetin, rhamnetin, isorhamnetin, apigenin, luteolin) were investigated using two short-term bacterial assays. In the "repair test" in Salmonella typhimurium (strains TA1538 uvrB- and TA1978 uvrB+) the flavonoids studied did not introduce any damage into the DNA recognized by UvrA...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::We here report the influence of the cell cycle abrogator UCN-01 on RKO human colon carcinoma cells differing in p53 status following exposure to two DNA damaging agents, the topoisomerase inhibitors etoposide and camptothecin. Cells were treated with the two drugs at the IC90 concentration for 24 h followed by post-in...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Fungal infections are a growing problem in contemporary medicine, yet only a few antifungal agents are used in clinical practice. In our laboratory we proposed the enzyme L-glutamine: D-fructose-6-phosphate amidotransferase (EC 2.6.1.16) as a new target for antifungals. The structure of this enzyme consists of two dom...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2005-01-01 00:00:00
abstract::A number of antiviral lectins, small proteins that bind carbohydrates found on viral envelopes, are currently in pre-clinical trials as potential drugs for prevention of transmission of human immunodeficiency virus (HIV) and other enveloped viruses, such as the Ebola virus and the coronavirus responsible for severe ac...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2006-01-01 00:00:00
abstract::In many plants belonging to angiosperms and gymnosperms the accumulation in leaves of long chain polyprenols and polyprenyl esters during growth in natural habitats depends on the light intensity. The amount of polyprenols in leaves is also positively correlated with the thickness of the leaf blade (SLA, specific leaf...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:055201233
更新日期:2005-01-01 00:00:00
abstract::beta-Secretase, a beta-site amyloid precursor protein (APP) cleaving enzyme (BACE), participates in the secretion of beta-amyloid peptides (Abeta), the major components of the toxic amyloid plaques found in the brains of patients with Alzheimer's disease (AD). According to the amyloid hypothesis, accumulation of Abeta...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:035001431
更新日期:2004-01-01 00:00:00
abstract::Stem cells (SC) research is an important part of biotechnology that could lead to the development of new therapeutic strategies. A lot of effort has been put to understand biology of the stem cells and to find genes and subsequently proteins that are responsible for their proliferation, self-renewal and differentiatio...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2005-01-01 00:00:00
abstract::The activity of polyhydric alcohol dehydrogenases in Mycobacterium sp. 279 was studied under limitation of zinc in the growth medium. It was found that the activity of ribitol and sorbitol dehydrogenases were markedly lowered and that of D-arabinitol dehydrogenase remained unchanged in the Zn2+-deficient cells. Other ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00