Abstract:
:So far, azide has been consistently reported to act as an inhibitor of metal enzymes, especially copper proteins. The present work shows that azide can also act as an activator of polyphenol oxidase II (PPO II) from tobacco leaves. From the square-wave voltammetry of native PPO II, peroxide-PPO II complex and azide-PPO II complex, the reduction of nitro blue tetrazolium by the enzymes and activation of PPO II by peroxide it follows that the binding of azide to PPO II induces the formation of CuO(2)(2-)Cu in the active site of PPO II from CuO(2)(-)Cu in native PPO II. The reason for azide acting as an activator can be attributed to azide complexing with PPO II, thus inducing the formation of CuO(2)(2-)Cu, which is the active site of the peroxide-PPO II complex in which peroxide plays the role of activator.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Shi C,Liu Q,Dai Y,Xie Y,Xu Xdoi
0249041029subject
Has Abstractpub_date
2002-01-01 00:00:00pages
1029-35issue
4eissn
0001-527Xissn
1734-154Xjournal_volume
49pub_type
杂志文章abstract::Nowadays, scientists may learn a lot about the organisms studied just by analyzing their genetic material. This requires the development of methods of reading genomes with high accuracy. It has become clear that the knowledge of the changes occurring within a viral genome is indispensable for effective fighting of the...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:045104983
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abstract::Changes in the composition of fatty acids (FA) were determined in lipid extracts isolated from developing ovaries containing ovules and developing seeds of Echium vulgare L. The samples were collected successively over 20 days beginning with the first day after flowering. The contents of the n-6 FA family members, i.e...
journal_title:Acta biochimica Polonica
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abstract::The base analogue 6-N-hydroxylaminopurine (HAP) is a potent mutagen in a variety of prokaryotic and eukaryotic organisms. Mutations in the yeast ham1 gene render the cells hypersensitive to the mutagenic effect of HAP. We have found that this gene has homologues in a variety of organisms from bacteria to man. We have ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:PURPOSE:To develop a novel intraarticular injection of diclofenac for the treatment of arthritis. METHOD:Diclofenac loaded nanoparticles were prepared by a nanoprecipitation technique using Eudragit L 100 as the polymer and polyvinyl alcohol as the surfactant. The nanoparticles were evaluated for particle size, zeta p...
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pub_type: 杂志文章
doi:10.18388/abp.2020_5401
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journal_title:Acta biochimica Polonica
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doi:
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
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journal_title:Acta biochimica Polonica
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doi:
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doi:
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2015_1111
更新日期:2016-01-01 00:00:00
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pub_type: 杂志文章
doi:10.18388/abp.2020_5088
更新日期:2020-03-17 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:035003691
更新日期:2003-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
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pub_type: 杂志文章,评审
doi:
更新日期:2006-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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journal_title:Acta biochimica Polonica
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doi:
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更新日期:2001-01-01 00:00:00
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doi:
更新日期:2011-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2014_825
更新日期:2015-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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doi:
更新日期:2007-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2008-01-01 00:00:00
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journal_title:Acta biochimica Polonica
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更新日期:1997-01-01 00:00:00
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