Abstract:
:We employed cDNA representational difference analysis (RDA) with human-mouse somatic hybrid cells containing human chromosome 17 and obtained several cDNA clones specific for this chromosome. A cDNA library from PHA-stimulated T cells was screened with unknown cDNA clones obtained by RDA as probes. Subsequently, 1 complete gene and 1 partial cDNA clone were obtained. Our successful result implies that this subtractive amplification technique with hybrid cells will be a useful aid in positional cloning in large-spanning regions.
journal_name
Genomicsjournal_title
Genomicsauthors
Tajima Y,Tashiro K,Camerini Ddoi
10.1006/geno.1997.4729subject
Has Abstractpub_date
1997-06-01 00:00:00pages
353-5issue
2eissn
0888-7543issn
1089-8646pii
S0888-7543(97)94729-7journal_volume
42pub_type
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