E-cadherin expression is a sensitive and specific method for detection of carcinoma cells in fluid specimens.

Abstract:

:The distinction between reactive mesothelial cells and carcinoma in pleural, peritoneal, and pericardial fluids is often difficult. We and others previously showed that E-cadherin, an epithelial-specific adhesion protein, can be useful for this distinction. In this study we tested the sensitivity and specificity of E-cadherin compared to, and in combination with, conventional cytology for assessment of carcinoma in fluids. Cytyc ThinPreptrade mark slides (Marlborough, MA) from 102 sequential fluids were evaluated for E-cadherin expression by routine immunologic techniques. No evidence of E-cadherin staining was seen in 71 cases, while 31 showed unequivocally positive staining. Sensitivity and specificity were independently determined for E-cadherin alone (72% and 97%, respectively), cytomorphology alone (62% and 100%, respectively), and both together (92% and 100%, respectively). We conclude that assessment of E-cadherin expression has sensitivity and specificity comparable to, or better than, conventional cytomorphology. If both cytomorphology and E-cadherin are used together, a definitive and correct diagnosis could have been made on nearly every case in this study.

journal_name

Diagn Cytopathol

journal_title

Diagnostic cytopathology

authors

Schofield K,D'Aquila T,Rimm DL

doi

10.1002/(sici)1097-0339(200005)22:5<263::aid-dc1>3

subject

Has Abstract

pub_date

2000-05-01 00:00:00

pages

263-7

issue

5

eissn

8755-1039

issn

1097-0339

pii

10.1002/(SICI)1097-0339(200005)22:5<263::AID-DC1>3

journal_volume

22

pub_type

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