Abstract:
BACKGROUND:Molecular testing is an essential step in providing patients with advanced non-small-cell lung cancer (NSCLC), the most appropriate front-line targeted therapies. We recently implemented targeted NGS on previously discarded cytology centrifuged supernatant (CCS). METHODS:In this study, we reviewed our implementation process to evaluate its performance. Performance and turnaround time (TAT) of molecular testing on all cytology NSCLC cases submitted for targeted NGS from June 2018 to September 2019 were evaluated, which included 46 and 62 cytology cases before and after implementation of CCS, respectively. Associated cost savings using CCS was also analyzed. RESULTS:The mean TAT defined as the time of collection to time of reporting was 8.5 ± 1.8 days in CCS cohort (range 5-13) as compared with 12.2 ± 5.3 days in the (FFPE) cell block (CB) cohort (range: 6-27). The success rate of sequencing was similar for both cohorts (100% in CCS and 96% in FFPE CB). CONCLUSION:Our results demonstrate that NGS using CCS improves TAT, preserves FFPE CB for other testing, and results in cost savings of $50 per case.
journal_name
Diagn Cytopatholjournal_title
Diagnostic cytopathologyauthors
Gokozan H,Harbhajanka A,Bomeisl P,Michael CW,Sadri Ndoi
10.1002/dc.24548subject
Has Abstractpub_date
2020-12-01 00:00:00pages
1167-1172issue
12eissn
8755-1039issn
1097-0339journal_volume
48pub_type
杂志文章abstract::The Bethesda System for Reporting Thyroid Cytopathology (TBSRTC) was formalized in October 2007 by experts in thyroidology at the National Institute of Health in Bethesda, Maryland. The first edition of the TBSRTC book was published in 2010 and the second edition in 2018. The TBSRTC is widely employed in cytology prac...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24385
更新日期:2020-10-01 00:00:00
abstract::Marginal zone B cell lymphomas (MZBCLs) represent a category of non-Hodgkin's lymphoma which may arise in a wide variety of extranodal organs where they are termed low grade B cell lymphomas of mucosa-associated lymphoid tissue (MALT). MZBCLs may involve primarily lymph nodes and or spleen where they are designated mo...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199904)20:4<190::aid-dc2>3
更新日期:1999-04-01 00:00:00
abstract::Sixty-six unselected breast cancers were analyzed in cytologic smears and histologic sections for the expression of Ki-67, proliferating cell nuclear antigen (PCNA), estrogen receptor protein (ERP), and p53 protein using a standard immunochemical method. The results, expressed as both positive cases and labelling inde...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840110206
更新日期:1994-01-01 00:00:00
abstract::Since calcific deposits in soft tissue may clinically resemble a tumor, it is feasible to investigate them by fine-needle aspiration (FNA) cytology. In this presentation we describe a case in the breast with extensive accumulation of acellular liquid calcific material which was sampled by the noninvasive technique of ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(200001)22:1<30::aid-dc9>3.
更新日期:2000-01-01 00:00:00
abstract::The diagnosis on fine needle aspiration of salivary gland tumors with a myoepithelial component is challenging because myoepithelial cells can have a wide cytomorphologic spectrum. The authors report a case of a pleomorphic adenoma of the parotid gland that expands the spectrum of appearances that myoepithelial cells ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24301
更新日期:2019-12-01 00:00:00
abstract::E-cadherin (E-CD) is a cell-adhesion molecule that has been associated with invasion and metastasis in a wide variety of human neoplasms. We have recently shown that, although decreased E-CD expression is associated with increased bladder-wall invasion and higher tumor grade of infiltrating transitional cell carcinoma...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0339(199605)14:4<310::AID-DC6>3
更新日期:1996-06-01 00:00:00
abstract::Epithelioid angiosarcomas are extremely rare tumors associated with poor prognosis and early metastases. Its epithelioid cytomorphology and limited vasoformation make it difficult to distinguish from more common malignancies, such as, carcinoma. This can be a potential diagnostic pitfall for the cytopathologist. In th...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21187
更新日期:2010-02-01 00:00:00
abstract::Lipoblastoma is an uncommon lipomatous tumor that typically occurs in infants and children. It may present as a single subcutaneous nodule or with multiple lesions (lipoblastomatosis). We describe fine-needle aspiration (FNA) cytologic features of two cases that presented as a subcutaneous lump in the scapular area an...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.20073
更新日期:2005-01-01 00:00:00
abstract::Although pleural and pericardial effusions are common and may rarely be the initial manifestation of disease, there are few reports of SLE diagnosed in a cytopathology laboratory. We describe an unusual case of SLE in a 75-year-old man who presented with cardiac tamponade. Cytologic examination of the pericardial flui...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199805)18:5<346::aid-dc8>3
更新日期:1998-05-01 00:00:00
abstract::Basal cell adenocarcinoma (BCAC) of salivary glands is uncommon low-grade malignancy. Only 19 cases of preoperative cytology were reported up to date. We present here a new case of BCAC arising in the minor salivary gland. Tumor was composed of clustered or isolated roundish, dark cells with small and regular nuclei. ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/dc.23919
更新日期:2018-08-01 00:00:00
abstract::Given the prevalence of human papilloma virus (HPV) infection, an attempt was made to determine whether certain factors such as keratinization and/or squamous atypia are associated with its development. Review of our gynecologic cytology files from 1989 yielded 1,615 specimens showing parakeratosis and/or hyperkeratos...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840120107
更新日期:1995-02-01 00:00:00
abstract::Metastatic dissemination is the primary cause of death in ovarian cancer (OvCa) patients, and dissemination to pleural and peritoneal effusions is a common clinical event. Effusion samples were collected from 15 OvCa patients. Twenty-six samples were collected prospectively, two were archival, and eight were taken fro...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20279
更新日期:2005-11-01 00:00:00
abstract::Angiolymphoid hyperplasia with eosinophilia (ALHE) is an uncommon, benign, inflammatory disorder involving the skin and microvasculature with an undetermined etiopathogenesis. Although the histopathology is well-characterized, the cytologic features of this entity have been rarely described in literature. This study w...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24563
更新日期:2021-01-01 00:00:00
abstract::Cytologic preparations were made from 53 biopsies of osseous and related lesions submitted for intraoperative diagnosis. Smears made by scraping the surface of the lesion and spreading the material obtained on a glass slide were most commonly used. These preparations were found to be valuable adjuncts to frozen sectio...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840020103
更新日期:1986-01-01 00:00:00
abstract::A 47-yr-old man with history of metastatic low-grade rectal leiomyosarcoma presented with progressive protrusion of his left eye due to an enlarging orbital mass. The differential diagnosis included tumor metastasis or orbital infection due to an unknown infectious agent. Diagnostic fine-needle aspiration (FNA) of the...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199711)17:5<369::aid-dc10>
更新日期:1997-11-01 00:00:00
abstract::To evaluate cytological atypical changes in apparently healthy oral mucosa exposed to smoking, alcohol, hot meals, and peppers using the AgNOR and Papanicolaou methods. A total of 180 individuals were evaluated, of which 60 were smokers, 34 were alcohol users, 52 were habitual peppers and hot meal (exposed) consumers,...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21224
更新日期:2010-07-01 00:00:00
abstract::Cultured anaplastic cell lines with previously characterized phenotypes are considered to be the best positive controls for immunocytochemistry. We assessed the validity of using anaplastic cell line cytospins as positive controls for immunocytochemistry performed on ThinPrep-processed clinical samples. We compared Th...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2059
更新日期:2001-11-01 00:00:00
abstract::Sebaceous lymphadenoma (SLA) is a histologic variant of lymphadenoma or sebaceous adenoma of the salivary gland. The fine-needle aspiration (FNA) cytology of SLA has not been well-described. In this report, we reported a case that involved an 81-year-old male with a long-standing left parotid mass and was diagnosed on...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23081
更新日期:2014-11-01 00:00:00
abstract::Like a pulmonary counterpart, extrapulmonary small cell carcinoma (SCC) is an aggressive tumor with a high rate of metastasis. Forty-nine fine-needle aspiration biopsies (FNABs) (36 patients) of various primary sites other than the lung diagnosed as metastatic SCC (including Merkel cell carcinoma) were reviewed. FNABs...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/(sici)1097-0339(199809)19:3<177::aid-dc4>3
更新日期:1998-09-01 00:00:00
abstract::Paget's disease of the vulva is a rare entity; since it may show the same clinical symptoms as benign chronic vulvitis, it may result in a delayed diagnosis. A 63-year-old woman was found to have an eczematoid pink to red lesion located in the vulva. Scraping cytology of the vulva showed isolated malignant cells of Pa...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21160
更新日期:2010-02-01 00:00:00
abstract::We report a rare case of extranodal NK/T-cell lymphoma, nasal type of the uterine cervix that showed cytologic features mimicking cervical cancer. A 65-year-old woman presented with vaginal bleeding. Gynecological examination revealed a bulky tumor of the cervix. A conventional Papanicolaou-stained cervical smear show...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.23439
更新日期:2016-05-01 00:00:00
abstract::This report details the cytologic features of primary localized amyloid tumor of the breast presenting as bilateral breast masses in a 72-yr-old woman. Clinically and radiographically, the masses simulated metastatic or multifocal carcinoma. Fine-needle aspiration revealed irregular globules of acellular amorphous mat...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840090521
更新日期:1993-10-01 00:00:00
abstract::Sclerosing stromal tumor (SST) of the ovary is a distinct subtype of sex cord-stromal tumor, which is derived from the ovarian stroma and, in turn, from the sex cords of the embryonic gonads. It is a benign tumor, predominantly, unilateral in nature and mostly involves the right side of the ovary. Histopathological fe...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21639
更新日期:2012-04-01 00:00:00
abstract::Nipple discharge (ND) either in the presence or in the absence of underlying mass is a very uncommon presentation in males and its bloody nature is still rarer. ND cytology in males has not been reported much in the literature. Spontaneous and expressed ND, however, can be very useful in establishing the diagnosis. Tw...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.24080
更新日期:2019-02-01 00:00:00
abstract::To determine the accuracy of fine-needle aspiration (FNA) in the diagnostic assessment of lymphadenopathies, a retrospective study was conducted on 627 cases of FNA of the lymph nodes performed at Department of Pathology, UNIFESP (Federal University of São Paulo), between 1997 and 2001. Cytology results were compared ...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20373
更新日期:2006-02-01 00:00:00
abstract::Monoclonal antibodies were used for a preoperative analysis of estrogen receptors (ERs) and progesterone receptors (PgRs) in fine-needle aspirates from 44 primary human breast carcinomas. The semiquantitative receptor values obtained in cytologic specimens correlated well with those from enzyme immunoassay analysis on...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.2840060205
更新日期:1990-01-01 00:00:00
abstract::A 53-yr-old woman with a 13-mo history of recurrent ovarian papillary serous adenocarcinoma presented with persistent microscopic hematuria. The patient was undergoing chemotherapy for her recurrent ovarian tumor when she was referred to the urology service for microscopic hematuria. An intravenous pyelogram was norma...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章,评审
doi:10.1002/(sici)1097-0339(199903)20:3<156::aid-dc8>3
更新日期:1999-03-01 00:00:00
abstract::Yolk sac tumors (YST) in extragonadal locations are rare. Cytologic diagnosis of YST on fine-needle aspiration (FNA) smears may be a challenge to the cytopathologist. Further neo-adjuvant therapy may be based on cytologic diagnosis making accuracy important. We studied FNA material from a hepatic mass in a pediatric p...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.20447
更新日期:2006-06-01 00:00:00
abstract::The Barr body is the inactive X chromosome in a female somatic cell. It is readily identified as plano-convex structure of 2-3 micron in diameter on the periphery of the nuclear membrane. The aim of this study is to evaluate the significance of Barr body count in malignant ovarian tumors on fine needle aspiration cyto...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.21688
更新日期:2012-11-01 00:00:00
abstract::We have shown that SurePath when compared to conventional Paps fails to increase HSIL detection. In this study, assessment of test performance characteristics for the FocalPoint showed that sensitivity was 96% when manual screening was used as the "gold standard." When cervical biopsy, however, was used as the "gold s...
journal_title:Diagnostic cytopathology
pub_type: 杂志文章
doi:10.1002/dc.10373
更新日期:2003-11-01 00:00:00