Abstract:
:Group B streptococcal (GBS) infections are associated with high morbidity and mortality. The molecular pathways mediating the pathophysiological events in GBS infection are not fully delineated. Cyclooxygenases (COX) are the enzymes that convert arachidonate to active eicosanoids. To identify the effects of GBS on eicosanoid metabolism and regulatory mechanisms, we exposed human monocytes to GBS and found that they secreted prostaglandin E2, prostacyclin, and thromboxane A2. Exposure to GBS caused monocytes to express COX-2 mRNA and protein in both a time- and concentration-dependent manner that correlated with eicosanoid production. COX-1 protein was unchanged. Addition of the anti-inflammatory cytokines interleukin (IL)-4 or IL-10 markedly attenuated GBS-induced COX-2 protein accumulation after GBS exposure, as did inhibition of p38 MAPK. Our experiments are the first to show that exposure of monocytes to a gram-positive bacterium (GBS) results in induction of functional COX-2, suggesting that eicosanoids may play important roles in the pathogenesis of GBS infections.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Maloney CG,Thompson SD,Hill HR,Bohnsack JF,McIntyre TM,Zimmerman GAdoi
10.1002/jlb.67.5.615subject
Has Abstractpub_date
2000-05-01 00:00:00pages
615-21issue
5eissn
0741-5400issn
1938-3673journal_volume
67pub_type
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