Abstract:
:Tumor necrosis factor alpha (TNF-alpha) has been shown to induce the production of interstitial collagenase by fibroblasts and chondrocytes. We investigated the role of TNF-alpha in collagenase gene expression by U937 monocyte/macrophage cells. Transcription of the TNF-alpha gene was observed after 0.5 h of phorbol myristate acetate (PMA) stimulation. Collagenase mRNA expression was not observed until 5-7 h of activation with PMA. TNF-alpha was detected in the culture supernatants 2-3 h before transcription of the collagenase gene. Neutralization of TNF-alpha protein with anti-TNF-alpha antibodies significantly reduced collagenase mRNA expression. Protein kinase C (PKC) and protein tyrosine kinase (PTK) inhibition essentially abolished both PMA-induced TNF-alpha protein secretion and collagenase mRNA expression. Collagenase gene expression induced by exogenous TNF-alpha in U937 cells stimulated with a suboptimal concentration of PMA was suppressed by PTK, but not PKC, inhibition. The pyrrolidine derivative of dithiocarbamate, a potent inhibitor of nuclear factor-kappa B (NF-kappa B) activation, resulted in a marked reduction in collagenase gene transcription, however, no reduction of TNF-alpha secretion was noted. Anti-TNF-alpha antibodies inhibited PMA-induced NF-kappa B activation. These observations demonstrate an important role for TNF-alpha in the autocrine regulation of collagenase gene expression by U937 cells. Additionally, TNF-alpha-induced PTK and NF-kappa B activation were important in collagenase gene expression in this cell line.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Callaghan MM,Lovis RM,Rammohan C,Lu Y,Pope RMdoi
10.1002/jlb.59.1.125subject
Has Abstractpub_date
1996-01-01 00:00:00pages
125-32issue
1eissn
0741-5400issn
1938-3673journal_volume
59pub_type
杂志文章abstract::The transcription factor nuclear factor (NF)-kappaB controls the expression of genes involved in inflammation, cell proliferation, apoptosis, and differentiation. Impaired regulation of NF-kappaB has been associated with many diseases; thus, there is significant interest in therapeutic approaches based on modulation o...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0405211
更新日期:2005-12-01 00:00:00
abstract::The effects of the carbocyclic nucleoside MDL 201,112 and the purine nucleoside adenosine on the interferon-gamma (IFN-gamma)-induced priming of macrophages (m phi s) for the respiratory burst and major histocompatibility class II (MHC class II) Ia+ antigen expression were compared. Priming of purified, peritoneal m p...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.56.2.133
更新日期:1994-08-01 00:00:00
abstract::The Fc gamma receptor IIB (FcγRIIB/CD32B) was generated million years ago during evolution. It is the sole inhibitory receptor for IgG, and has long been associated with the regulation of humoral immunity and innate immune homeostasis. However, new and surprising functions of FcγRIIB are emerging. In particular, FcγRI...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1002/JLB.2MIR0917-354R
更新日期:2018-02-06 00:00:00
abstract::In this report, the construction of a functional, immunocompetent, full-thickness skin equivalent (SE) is described, consisting of an epidermal compartment containing keratinocytes, melanocytes, and human LCs derived from the MUTZ-3 cell line (MUTZ-LC) and a fibroblast-populated dermal compartment. The CD1a(+)Langerin...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0610374
更新日期:2011-11-01 00:00:00
abstract::Nitric oxide (NO), the molecule of the year 1992, is gaining recognition as an important biological mediator. Its multitude of physiologic and pathophysiologic functions result from both a wide distribution of synthesis and diverse mechanisms of action. Besides its functions as a potent vasodilator and neurotransmitte...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:
更新日期:1993-08-01 00:00:00
abstract::Stimulation of phagocytic leukocytes with chemotactic factors results in transient acidification, followed by alkalinization of the cytosol. Human monocytes are known to alter their functional responses to the chemotactic peptide N-formylmethionyl-leucyl-phenylalanine (fMLP) in a complex fashion as they mature in vitr...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.53.6.673
更新日期:1993-06-01 00:00:00
abstract::The transcription factor GATA-2 is expressed in hematopoietic stem and progenitor cells and is functionally implicated in their survival and proliferation. In the present study, we show that GATA-2 exists as an acetylated protein in immature precursor cells, KG1. GATA-2 was acetylated in vitro by p300 and GCN5. We hav...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0603389
更新日期:2004-03-01 00:00:00
abstract::DCs orchestrate immune responses contributing to the pattern of response developed. In cancer, DCs may play a dysfunctional role in the induction of CD4(+)CD25(+)Foxp3(+) Tregs, contributing to immune evasion. We show here that Mo-DCs from breast cancer patients show an altered phenotype and induce preferentially Treg...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0112048
更新日期:2012-09-01 00:00:00
abstract::Retroviral infections are frequently associated with immunosuppression. Retroviral transmembrane envelope proteins (TM proteins) play an important role in this phenomenon. CKS-17, a synthetic heptadecapeptide, represents the immunosuppressive site of these retroviral TM proteins. Here we support on the further delinea...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.51.3.282
更新日期:1992-03-01 00:00:00
abstract::Bovine plasma contains factors that can stimulate bovine neutrophils. Bovine plasma at 1:1,000,000-1:1,000 dilution induced a dose-dependent superoxide production in bovine neutrophils. When bovine plasma was fractionated with a protein G column, only the IgG fraction contained induction activity. At similar concentra...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.58.2.203
更新日期:1995-08-01 00:00:00
abstract::The objective of this study was to find the role of LncRNA SNHG12 in the regulation of hypertensive vascular endothelial injury. LncRNA SNHG12 and miR-25-3p expression were detected by quantitative RT-PCR. Protein levels of Sirtuin 6 (SIRT6), endothelial cell (EC) senescence markers p16 and p21, and EC marker CD31 wer...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.1A0820-501R
更新日期:2021-01-19 00:00:00
abstract::Postnatal stem cells regulate the homeostasis of the majority of our tissues. They continuously generate new progenitors and mature, functional cells to replace old cells, which cannot assume the tissue function anymore and are eliminated. Blood, skin, gut mucosa, muscle, cartilage, nerves, cornea, retina, liver, and ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.0505272
更新日期:2005-10-01 00:00:00
abstract::The immune system is armed with a broad range of receptors to detect and initiate the elimination of bacterial pathogens. Inflammasomes are molecular platforms that sense a diverse range of microbial insults to develop appropriate host response. In that context, noncanonical inflammasome arose as a sensor for Gram-neg...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1002/JLB.4MR0119-013R
更新日期:2019-09-01 00:00:00
abstract::It is well established that the cytokine IL-12 and the transcription factor STAT4, an essential part of the IL-12 signaling pathway, are critical components of the Th1 differentiation process in T cells. In response to pathogenic stimuli, this process causes T cells to proliferate rapidly and secrete high amounts of t...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1A0814-375RR
更新日期:2015-10-01 00:00:00
abstract::When mouse peritoneal cells in early phase-inflammatory exudates were cultured in vitro, the number of polymorphonuclear leukocytes (PMNs) rapidly decreased, whereas the number of macrophages gradually increased during culture. Macrophage growth was also induced by addition of effete PMNs or their debris to macrophage...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.39.5.489
更新日期:1986-05-01 00:00:00
abstract::Galectin-3 (gal-3), a beta-galactoside-binding animal lectin, plays a role in cell-cell and cell-extracellular matrix interactions. Extracellular gal-3 modulates cell migration and adhesion in several physiological and pathological processes. Gal-3 is highly expressed in activated macrophages. Schistosoma mansoni eggs...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1206747
更新日期:2007-08-01 00:00:00
abstract::TLR are primary triggers of the innate immune system by recognizing various microorganisms through conserved pathogen-associated molecular patterns. TLR2 is the receptor for a functional recognition of bacterial lipopeptides (LP) and is up-regulated during various disorders such as chronic obstructive pulmonary diseas...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0807586
更新日期:2008-03-01 00:00:00
abstract::Patients with head and neck squamous cell carcinoma (HNSCC) have increased levels of immune-suppressive peripheral blood CD34+ cells. This study showed that the peripheral blood CD34+ cells of HNSCC patients are capable of differentiating into dendritic cells. Because CD34+ cells can differentiate through several path...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.65.5.623
更新日期:1999-05-01 00:00:00
abstract::Compared with CD4+25+ regulatory T cells (Tregs), the mechanisms for natural, polyclonal CD8+25+ Treg immune suppression have been significantly less studied. We previously showed that polyclonal T cells can acquire antigen-specific targeting activity through arming with exosomal peptide-MHC (pMHC). In this study, we ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.3A0716-295RR
更新日期:2017-05-01 00:00:00
abstract::As part of a study to identify novel genes associated with macrophage activation, we have cloned a new member of the transforming growth factor beta (TGF-beta) superfamily designated macrophage inhibitory cytokine 1 (MIC-1). MIC-1 is synthesized as a 62-kDa intracellular protein, which, after cleavage by a furin like ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1002/jlb.65.1.2
更新日期:1999-01-01 00:00:00
abstract::MΦs are important sensory cells of the innate immune system and regulate immune responses through releasing different combinations of cytokines. In this study, we examined whether cytokines released by MΦs in response to the probiotic bacterial strain GR-1 modulate the responses of DCs. The cytokine profile released b...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0810445
更新日期:2011-06-01 00:00:00
abstract::Toll-like receptor 2 (TLR2) and -4 mediate signals from a great variety of bacterial gut products, giving the host a panel of microbe-recognizing receptors. Under homeostatic conditions, TLRs act as protective receptors of the intestinal epithelium. When homeostasis is disrupted in diseases such as inflammatory bowel ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1004564
更新日期:2005-08-01 00:00:00
abstract::The role of NOD2 and RIP2 in inflammatory disease has been paradoxical. Whereas loss-of-function NOD2 polymorphisms cause CD, a granulomatous disease of the gastrointestinal tract, gain-of-function mutations cause EOS-a granulomatous disease primarily affecting the skin, joints, and eyes. Thus, gain-of-function mutati...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.0213109
更新日期:2013-11-01 00:00:00
abstract::The ganglioside composition of endotoxin-responsive C3H/HeN murine peritoneal macrophages is known to undergo dramatic changes in vivo in response to intraperitoneal lipopolysaccharides (LPS), unlike endotoxin-hyporesponsive C3H/HeJ macrophages. To better investigate the mechanism behind LPS-induced macrophage ganglio...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.45.3.221
更新日期:1989-03-01 00:00:00
abstract::The mannose receptor is a macrophage surface receptor that mediates both endocytosis and phagocytosis. Previous work has demonstrated that the prototypical Th 2 cytokine, interleukin-4 (IL-4), increases both cell-surface receptor expression and mannose receptor-mediated endocytosis, whereas the prototypical Th 1 cytok...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.64.1.108
更新日期:1998-07-01 00:00:00
abstract::Multiple mechanisms are used by the human immunodeficiency virus type 1 (HIV-1) to interfere with host-cell immune effector functions. The 27-kD Nef protein has been shown to down-modulate specific genes of the major histocompatibility complex class I (MHC-I) on the surface of infected primary T cells, facilitating th...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0505237
更新日期:2005-09-01 00:00:00
abstract::We provide new information on how apoptosis regulates the expansion and survival of dendritic cell (DC) elements during in vitro hematopoiesis. Functionally distinct apoptotic schedules were associated with different phases of DC development when multipotent CD34+ progenitor cells were treated with GM-CSF + TNF +/- SC...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.62.4.493
更新日期:1997-10-01 00:00:00
abstract::Pulmonary macrophages (Mphi) increase in tissue and bronchoalveolar lavage (BAL) fluid during inflammation caused by bleomycin (BLM). This study demonstrates that increasing numbers of exudate Mphi in BLM lung injury exhibit dendritic cell (DC) features. After the intratracheal administration of BLM (0.075 U), adheren...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.66.6.901
更新日期:1999-12-01 00:00:00
abstract::We have characterized the beta-adrenergic receptor binding site and the beta-adrenergic cAMP response of the HL-60 cell. The hydrophilic ligand [3H]-(-)-CGP-12177 was specifically and reversibly bound to one single class of binding sites (Kd 220 pM and Bmax 1,970 sites/cell). The adrenergic agonists inhibited the spec...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.44.1.41
更新日期:1988-07-01 00:00:00
abstract::Breaking the long-held paradigm that primary B cells are not phagocytic, several studies have demonstrated recently that B cells from fish, amphibians, and reptilians have a significant phagocytic capacity. Whether such capacity has remained conserved in certain mammalian B cell subsets is presently an enigma. Here, w...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0711372
更新日期:2012-04-01 00:00:00