Abstract:
:The transcription factor GATA-2 is expressed in hematopoietic stem and progenitor cells and is functionally implicated in their survival and proliferation. In the present study, we show that GATA-2 exists as an acetylated protein in immature precursor cells, KG1. GATA-2 was acetylated in vitro by p300 and GCN5. We have identified multiple acetylation sites by p300 on GATA-2, which include sites outside the zinc finger domain. We confirmed that GATA-2 acetylation occurred in transiently transfected 293T cells at sites similar to those induced by p300 in vitro. We have successfully shown that acetylation of GATA-2 in vitro increased its DNA-binding activity. In addition, GATA-2 displayed a transcriptional synergism with p300 that was impaired by mutation of each acetylation site. More importantly, each mutation in the acetylation sites of GATA-2 abolished its growth inhibitory effect on an interleukin-3-dependent progenitor, 32D. We conclude that acetylation provides multiple control points for the regulation of GATA-2 function.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Hayakawa F,Towatari M,Ozawa Y,Tomita A,Privalsky ML,Saito Hdoi
10.1189/jlb.0603389subject
Has Abstractpub_date
2004-03-01 00:00:00pages
529-40issue
3eissn
0741-5400issn
1938-3673pii
jlb.0603389journal_volume
75pub_type
杂志文章abstract::Upon exposure to immune or inflammatory stimuli, dendritic cells (DC) migrate from peripheral tissues to lymphoid organs, where they present antigen. The molecular basis for the peculiar trafficking properties of DC is largely unknown. In this study, mouse DC were generated from CD34+ bone marrow precursors and cultur...
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journal_title:Journal of leukocyte biology
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