Interleukin-1 beta (IL-1 beta), IL-1 receptor antagonist, and TNF alpha production in whole blood.

Abstract:

:The ability of an individual to mount defense responses to infection depend in part on the capacity to produce cytokines such as interleukin 1 (IL-1) and tumor necrosis factor (TNF). The specialized equipment, labor intensity, and sterile practice required for the standard in vitro evaluation of cytokine production can make such evaluation impractical in some clinical situations. We report a method for stimulating whole blood to produce cytokines that can be implemented in laboratories without tissue culture facilities and requires minimal sample preparation. IL-1 beta and TNF alpha production in whole blood samples was stimulated with endotoxin and/or phytohemagglutinin in standard EDTA-containing vacuum collection tubes. After incubation, plasma was removed and frozen for later assay. Comparison of this whole blood method with isolated mononuclear cell cultures indicated a significant correlation for IL-1 beta production (r = 0.746, P = 0.005). This technique also produced the newly described cytokine, IL-1 receptor antagonist. We conclude that the whole blood method is an acceptable alternative to isolated cell culture methods for measuring IL-1 beta in situations that preclude the standard in vitro approach.

journal_name

J Leukoc Biol

authors

Nerad JL,Griffiths JK,Van der Meer JW,Endres S,Poutsiaka DD,Keusch GT,Bennish M,Salam MA,Dinarello CA,Cannon JG

doi

10.1002/jlb.52.6.687

subject

Has Abstract

pub_date

1992-12-01 00:00:00

pages

687-92

issue

6

eissn

0741-5400

issn

1938-3673

journal_volume

52

pub_type

杂志文章
  • Respiratory viral infection, epithelial cytokines, and innate lymphoid cells in asthma exacerbations.

    abstract::Exacerbations of asthma are most commonly triggered by viral infections, which amplify allergic inflammation. Cytokines released by virus-infected AECs may be important in driving this response. This review focuses on accumulating evidence in support of a role for epithelial cytokines, including IL-33, IL-25, and TSLP...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1189/jlb.3RI0314-129R

    authors: Kumar RK,Foster PS,Rosenberg HF

    更新日期:2014-09-01 00:00:00

  • Leukocytes of the intestinal muscularis: their phenotype and isolation.

    abstract::The basal presence of immunologically potent cells within the intestinal muscularis externa and their functional significance is unclear. Our aim was to investigate the basal distribution of various leukocyte populations within the rat jejunal muscularis. In addition, we sought to immunohistochemically phenotype the m...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.63.6.683

    authors: Kalff JC,Schwarz NT,Walgenbach KJ,Schraut WH,Bauer AJ

    更新日期:1998-06-01 00:00:00

  • Transferrin receptor in T cell activation and transplantation.

    abstract::Transferrin receptor (TfR) expression is up-regulated during T cell activation after the interaction of the T cell receptor with the antigen-major histocompatibility complex and the expression of interleukin-2 (IL-2) receptor. We hypothesize that anti-TfR monoclonal antibody (mAb) will prolong allograft survival by al...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.64.1.19

    authors: Bayer AL,Baliga P,Woodward JE

    更新日期:1998-07-01 00:00:00

  • Th 1 and Th 2 cytokines cooperate to stimulate mannose-receptor-mediated phagocytosis.

    abstract::The mannose receptor is a macrophage surface receptor that mediates both endocytosis and phagocytosis. Previous work has demonstrated that the prototypical Th 2 cytokine, interleukin-4 (IL-4), increases both cell-surface receptor expression and mannose receptor-mediated endocytosis, whereas the prototypical Th 1 cytok...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.64.1.108

    authors: Raveh D,Kruskal BA,Farland J,Ezekowitz RAB

    更新日期:1998-07-01 00:00:00

  • Stromal cell-derived factor 1-alpha (SDF)-induced human T cell chemotaxis becomes phosphoinositide 3-kinase (PI3K)-independent: role of PKC-theta.

    abstract::Stromal cell-derived factor 1alpha (SDF-1alpha) is the exclusive ligand for the chemokine receptor CXCR4. This receptor plays a pivotal role in immune responses, the pathogenesis of infection such as HIV, and cellular trafficking. However, the signaling mechanisms regulating SDF-driven T cell migration are not well de...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0607420

    authors: Shahabi NA,McAllen K,Sharp BM

    更新日期:2008-03-01 00:00:00

  • H2 complex controls CD4/CD8 ratio, recurrent responsiveness to repeated stimulations, and resistance to activation-induced apoptosis during T cell response to mycobacterial antigens.

    abstract::Genetic variation in the major histocompatibility complex (MHC) influences susceptibility and immune responses to Mycobacterium tuberculosis in mice and humans, but connections among the severity of tuberculosis (TB), dynamic changes in T cell responses to mycobacteria, and MHC genetic polymorphisms are poorly charact...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0705392

    authors: Pichugin AV,Petrovskaya SN,Apt AS

    更新日期:2006-04-01 00:00:00

  • Inhibitors of DNA methylation and histone deacetylation independently relieve AML1/ETO-mediated lysozyme repression.

    abstract::The human lysozyme (LZM) gene is highly methylated in LZM-nonexpressor immature myeloid and in nonmyeloid cells and unmethylated only in LZM-expressing cells. Extended methylation analyses of the CpG-poor 5' flanking region and of the exon 4 CpG island (both containing Alu elements) of the LZM gene were now performed....

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0106005

    authors: Claus R,Fliegauf M,Stock M,Duque JA,Kolanczyk M,Lübbert M

    更新日期:2006-12-01 00:00:00

  • Prolonged neutrophil survival at necrotic sites is a fundamental feature for tissue recovery and resolution of hepatic inflammation.

    abstract::Neutrophils were classically described as powerful effectors of acute inflammation, and their main purpose was assumed to be restricted to pathogen killing through production of oxidants. As consequence, neutrophils also may lead to significant collateral damage to the healthy tissues, and after performing these tasks...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/JLB.1MA0420-634R

    authors: Mattos MS,Lopes ME,de Araujo AM,Alvarenga DM,Nakagaki BN,Mafra K,de Miranda CDM,Diniz AB,Antunes MM,Lopes MAF,Rezende RM,Menezes GB

    更新日期:2020-10-01 00:00:00

  • An essential role for IFN-β in the induction of IFN-stimulated gene expression by LPS in macrophages.

    abstract::TLR agonists such as LPS and poly(I:C) induce expression of type I IFNs, such as IFN-α and -β, by macrophages. To examine the role of IFN-β in the induction of ISGs by LPS, we compared the ability of LPS to induce ISGF3 activity and ISG expression in bone marrow-derived macrophages from WT and Ifnb1(-/-) mice. We foun...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.2A0414-191R

    authors: Sheikh F,Dickensheets H,Gamero AM,Vogel SN,Donnelly RP

    更新日期:2014-10-01 00:00:00

  • Cells under stress: The mechanical environment shapes inflammasome responses to danger signals.

    abstract::Many intracellular signals, such as host danger-associated molecules and bacterial toxins during infection, elicit inflammasome activation. However, the mechanical environment in tissues may also influence the sensitivity of various inflammasomes to activation. The cellular mechanical environment is determined by the ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1002/JLB.3MIR1118-417R

    authors: Joshi H,Morley SC

    更新日期:2019-07-01 00:00:00

  • Subacute treatment of carprofen facilitate splenocardiac resolution deficit in cardiac injury.

    abstract::Inflammation-limiting nonsteroidal pain relievers magnify myocardial infarction (MI) incidences and increase re-admission events in heart failure (HF) patients. However, the molecular and cellular mechanism of this provocative adverse effect is unclear. Our goal was to determine whether carprofen (CAP) impedes splenic...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/JLB.3A0618-223R

    authors: Halade GV,Kain V,Wright GM,Jadapalli JK

    更新日期:2018-12-01 00:00:00

  • Urinary levels of chemokines (MCAF/MCP-1, IL-8) reflect distinct disease activities and phases of human IgA nephropathy.

    abstract::Leukocytes have been implicated to be involved in the pathogenesis of IgA nephropathy (IgAN). To clarify the precise molecular mechanism of recruitment and activation of leukocytes in the subgroups of IgAN, latent, acute, and chronic types, we studied monocyte chemotactic and activating factor (MCAF/MCP-1) and interle...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.63.4.493

    authors: Yokoyama H,Wada T,Furuichi K,Segawa C,Shimizu M,Kobayashi K,Su S,Mukaida N,Matsushima K

    更新日期:1998-04-01 00:00:00

  • Activation-induced expression of CD1d antigen on mature T cells.

    abstract::In the present study, we investigated the expression of human CD1d antigen on activated mature T cells. Expression of this glycoprotein was found to be highly regulated and dependent on PHA stimulation. Flow cytometry studies using the NOR3.2 antibody, which recognized CD1d under denaturing conditions, showed a clear ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:

    authors: Salamone MC,Rabinovich GA,Mendiguren AK,Salamone GV,Fainboim L

    更新日期:2001-02-01 00:00:00

  • Human microglial cells have phenotypic and functional characteristics in common with both macrophages and dendritic antigen-presenting cells.

    abstract::Resting microglia comprise up to 13% of the cells in human central nervous system (CNS) white matter. Their large number and dendritic morphology make them ideally suited to survey the CNS for noxious stimuli. Upon activation microglia gradually lose dendritic processes and transform into typical phagocytic macrophage...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.56.6.732

    authors: Ulvestad E,Williams K,Bjerkvig R,Tiekotter K,Antel J,Matre R

    更新日期:1994-12-01 00:00:00

  • Chronic alcohol ingestion modulates hepatic macrophage populations and functions in mice.

    abstract::Hepatic Macs, consisting of resident KCs and infiltrating monocytes/IMs, are thought to play an important role in the pathogenesis of ALD. Previous work has focused on KCs or studied hepatic Macs as one cell population. The aim of the current study is to distinguish IMs from KCs and to compare their phenotypes and fun...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.6A0114-004RR

    authors: Wang M,You Q,Lor K,Chen F,Gao B,Ju C

    更新日期:2014-10-01 00:00:00

  • Human CD8+ T cells recognize epitopes of the 28-kDa hemolysin and the 38-kDa antigen of Mycobacterium tuberculosis.

    abstract::Mycobacterium tuberculosis antigens that are recognized by human CD8+ T cells are potentially important vaccine target molecules. We used a motif-based strategy to screen selected proteins of M. tuberculosis for peptides predicted to bind to human leukocyte antigen (HLA)-A*0201. We identified two 10 amino acid peptide...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0403138

    authors: Shams H,Barnes PF,Weis SE,Klucar P,Wizel B

    更新日期:2003-12-01 00:00:00

  • EBI2 in splenic and local immune responses and in autoimmunity.

    abstract::The seven transmembrane G protein-coupled receptor EBV-induced gene 2 (EBI2), also known as GPR183, is expressed in particular in immune cells. Activated by its endogenous ligands, which are a group of oxysterols, it functions as a chemo-attractant receptor, mediating cell migration. In coordination with other recepto...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1002/JLB.2VMR1217-510R

    authors: Barington L,Wanke F,Niss Arfelt K,Holst PJ,Kurschus FC,Rosenkilde MM

    更新日期:2018-08-01 00:00:00

  • Expression and functional characterization of CFTR in mast cells.

    abstract::Mast cell activation requires Cl(-) flux, which maintains the driving force for entry of extracellular calcium and initiates release of mediators such as histamine. However, chloride channel expression in mast cells has been poorly understood. For the first time, reverse transcriptase-polymerase chain reaction shows t...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:

    authors: Kulka M,Gilchrist M,Duszyk M,Befus AD

    更新日期:2002-01-01 00:00:00

  • Oxidative modifications of S100 proteins: functional regulation by redox.

    abstract::Several S100 Ca(2+)-binding proteins undergo various post-translational modifications that may alter their intracellular and extracellular functions. S100A8 and S100A9, two members of this family, are particularly susceptible to oxidative modification. These proteins, abundantly expressed in neutrophils and activated ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1189/jlb.1008608

    authors: Lim SY,Raftery MJ,Goyette J,Hsu K,Geczy CL

    更新日期:2009-09-01 00:00:00

  • Macrophage differentiation and expression of macrophage colony-stimulating factor in murine milky spots and omentum after macrophage elimination.

    abstract::To elucidate the differentiation mechanisms of macrophages in the murine omentum, we studied the repopulation of these cells and the expression of macrophage colony-stimulating factor (M-CSF) in the milky spots and omental tissues in mice depleted of macrophages following administration of liposome-encapsulated dichlo...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.61.4.436

    authors: Zhu H,Naito M,Umezu H,Moriyama H,Takatsuka H,Takahashi K,Shultz LD

    更新日期:1997-04-01 00:00:00

  • Differential production of IFN-alpha/beta by CSF-1- and GM-CSF-derived macrophages.

    abstract::Mature macrophages, derived in vitro from bone marrow progenitors under the influence of either macrophage colony stimulating factor (CSF-1) or granulocyte-macrophage (GM)-CSF, have been shown to differ morphologically and functionally. The data presented in this report demonstrate that macrophages derived from bone m...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.48.1.43

    authors: Falk LA,Vogel SN

    更新日期:1990-07-01 00:00:00

  • Dissociation between the translocation and the activation of Akt in fMLP-stimulated human neutrophils--effect of prostaglandin E2.

    abstract::PGE(2) and other cAMP-elevating agents are known to down-regulate most functions stimulated by fMLP in human polymorphonuclear neutrophils. We reported previously that the inhibitory potential of PGE(2) resides in its capacity to suppress fMLP-stimulated PI-3Kgamma activation via the PGE(2) receptor EP(2) and hence, t...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0406256

    authors: Burelout C,Naccache PH,Bourgoin SG

    更新日期:2007-06-01 00:00:00

  • Aspergillus fumigatus antigen induced eosinophilia in mice is abrogated by anti-IL-5 antibody.

    abstract::A murine model of allergic bronchopulmonary aspergillosis (ABPA), developed by exposure to Aspergillus fumigatus antigens, demonstrated eosinophilia of peripheral blood (PB), bone marrow (BM), and lung. The eosinophilia was abrogated by monoclonal anti-interleukin-5 (IL-5) antibody (TRFK-5) and not by an isotype contr...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.53.3.264

    authors: Murali PS,Kumar A,Choi H,Banasal NK,Fink JN,Kurup VP

    更新日期:1993-03-01 00:00:00

  • The functional role of surface molecules on extracellular vesicles in cancer, autoimmune diseases, and coagulopathy.

    abstract::Extracellular vesicles (EVs) are nanosized particles that have emerged as mediators for intercellular communication in physiologic and pathologic conditions. EVs carry signaling information on their bilipid membrane as well as cargo within, allowing them to perform a wide range of biologic processes and contribute to ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1002/JLB.3MR0420-067R

    authors: Lam KCK,Lam MKN,Chim CS,Chan GCF,Li JCB

    更新日期:2020-11-01 00:00:00

  • TREM-like transcript 2 is stored in human neutrophil primary granules and is up-regulated in response to inflammatory mediators.

    abstract::The triggering receptor expressed on myeloid cell locus encodes a family of receptors that is emerging as an important class of molecules involved in modulating the innate immune response and inflammation. Of the 4 conserved members, including triggering receptor expressed on myeloid cells 1 and 2 and triggering recep...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.3AB1115-507R

    authors: Thomas KA,King RG,Sestero CM,Justement LB

    更新日期:2016-07-01 00:00:00

  • CD56bright NK IL-7Rα expression negatively associates with HCV level, and IL-7-induced NK function is impaired during HCV and HIV infections.

    abstract::Several lines of evidence support the concept that NK cells play an important role in control of hepatitis C virus (HCV) infection via cytokine secretion and cytotoxicity. IL-7 is a homeostatic cytokine with a role in T cell development, activation, proliferation, and cytokine secretion. The IL-7Rα chain [cluster of d...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.5A1116-456R

    authors: Judge CJ,Kostadinova L,Sherman KE,Butt AA,Falck-Ytter Y,Funderburg NT,Landay AL,Lederman MM,Sieg SF,Sandberg JK,Anthony DD

    更新日期:2017-07-01 00:00:00

  • Inflammation programs self-reactive CD8+ T cells to acquire T-box-mediated effector function but does not prevent deletional tolerance.

    abstract::CD8(+) T cells must detect foreign antigens and differentiate into effector cells to eliminate infections. But, when self-antigen is recognized instead, mechanisms of peripheral tolerance prevent acquisition of effector function to avoid autoimmunity. These distinct responses are influenced by inflammatory and regulat...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.1A0913-500RR

    authors: Jackson SR,Yuan J,Berrien-Elliott MM,Chen CL,Meyer JM,Donlin MJ,Teague RM

    更新日期:2014-09-01 00:00:00

  • Partial characterization of a mitomycin-C resistant T cell.

    abstract::A cell resistant to concentrations of mitomycin-C associated with the inactivation of many in vitro immune responses is described. This cell is responsive to concanavalin A (Con A), poorly adherent to nylon wool, present in relatively greater numbers in lymph node than other lymphoid tissues, and is relatively radiose...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.41.1.33

    authors: Anderson RE,Standefer JC,Troup GM,Pogue LE

    更新日期:1987-01-01 00:00:00

  • Calcium/calmodulin-dependent protein kinase (CaMK) Ialpha mediates the macrophage inflammatory response to sepsis.

    abstract::Dysregulated Ca(2+) handling is prevalent during sepsis and postulated to perpetuate the aberrant inflammation underlying subsequent organ dysfunction and death. The signal transduction cascades mediating these processes are unknown. Here, we identify that CaMKIα mediates the Mϕ response to LPS in vitro and the inflam...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0510286

    authors: Zhang X,Guo L,Collage RD,Stripay JL,Tsung A,Lee JS,Rosengart MR

    更新日期:2011-08-01 00:00:00

  • Mycoplasma stimulates the production of oxidative radicals by murine peritoneal macrophages.

    abstract::Mycoplasmas and mycoplasma membranes have been shown to induce the production of inflammatory cytokines, including tumor necrosis factor alpha and interleukin-6, as well as nitric oxide, by mouse macrophages and rat brain astrocytes. Luminol-enhanced chemiluminescence was used as a sensitive method to show that Mycopl...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.57.2.264

    authors: Avron A,Gallily R

    更新日期:1995-02-01 00:00:00