Chronic alcohol ingestion modulates hepatic macrophage populations and functions in mice.

Abstract:

:Hepatic Macs, consisting of resident KCs and infiltrating monocytes/IMs, are thought to play an important role in the pathogenesis of ALD. Previous work has focused on KCs or studied hepatic Macs as one cell population. The aim of the current study is to distinguish IMs from KCs and to compare their phenotypes and functions. We show here that a 4-week ethanol feeding of C57BL/6J mice causes recruitment of IMs into the liver. KCs and IMs can be distinguished based on their differential expression of F4/80 and CD11b. IMs can be divided further into two subsets based on their differential expression of Ly6C. KCs and two subsets of IMs were separately purified by FACS. The phagocytosis abilities and the expression profiles of genes related to various functions were compared among different populations of hepatic Macs. Ly6C(low) IMs exhibit an anti-inflammatory and tissue-protective phenotype; in contrast, Ly6C(hi) IMs exhibit a proinflammatory, tissue-damaging phenotype. The ratio of Ly6C(hi)/Ly6C(low) increases when mice chronically fed ethanol were binged, which significantly enhanced liver injury. Moreover, upon phagocytosis of apoptotic hepatocytes, Ly6C(hi) IMs switch to Ly6C(low) IMs. Taken together, chronic ethanol feeding induces the recruitment of two subsets of hepatic IMs, which play different or even opposite roles in regulating liver inflammation and repair. These findings may not only increase our understanding of the complex functions of Macs in the pathogenesis of ALD but also help us to identify novel therapeutic targets for the treatment of this disease.

journal_name

J Leukoc Biol

authors

Wang M,You Q,Lor K,Chen F,Gao B,Ju C

doi

10.1189/jlb.6A0114-004RR

subject

Has Abstract

pub_date

2014-10-01 00:00:00

pages

657-65

issue

4

eissn

0741-5400

issn

1938-3673

pii

jlb.6A0114-004RR

journal_volume

96

pub_type

杂志文章
  • An eosinophil immune response characterizes the inflammatory skin disease observed in Tie-2 transgenic mice.

    abstract::Although mouse models of inflammatory skin diseases such as psoriasis and atopic dermatitis fail to completely phenocopy disease in humans, they provide invaluable tools to examine the molecular and cellular mechanisms responsible for the epidermal hyperplasia, inflammation, and excess angiogenesis observed in human d...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0607347

    authors: Voskas D,Babichev Y,Ling LS,Alami J,Shaked Y,Kerbel RS,Ciruna B,Dumont DJ

    更新日期:2008-07-01 00:00:00

  • Prenylation of an interferon-gamma-induced GTP-binding protein: the human guanylate binding protein, huGBP1.

    abstract::Interferons (IFN) and lipopolysaccharide (LPS) cause multiple changes in isoprenoid-modified proteins in murine macrophages, the most dramatic being the expression of a prenyl protein of 65 kDa. The guanylate binding proteins (GBPs) are IFN-inducible GTP-binding proteins of approximately 65 kDa that possess a CaaX mot...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.60.3.423

    authors: Nantais DE,Schwemmle M,Stickney JT,Vestal DJ,Buss JE

    更新日期:1996-09-01 00:00:00

  • Annexin A2 is a soluble mediator of macrophage activation.

    abstract::On the surface of the macrophage, annexin A2 tetramer (A2t) serves as a docking protein or recognition element for bacterial and viral pathogens. Plasma levels of free A2t have been reported to increase following infection, although the mechanistic significance of this observation is unclear. Although annexin A2 had g...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0307154

    authors: Swisher JF,Khatri U,Feldman GM

    更新日期:2007-11-01 00:00:00

  • Macrophage priming by interferon gamma: a selective process with potentially harmful effects.

    abstract::The tissue-fixed macrophage is a key cellular element in the initiation and regulation of inflammation. Understanding the regulation of macrophage activation may provide valuable clues to the mechanisms involved in both beneficial and deleterious effects of inflammation. The lymphokine interferon-gamma (IFN-gamma) is ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.52.6.579

    authors: Williams JG,Jurkovich GJ,Hahnel GB,Maier RV

    更新日期:1992-12-01 00:00:00

  • gp130 at the nexus of inflammation, autoimmunity, and cancer.

    abstract::Glycoprotein 130 (gp130) is a shared receptor utilized by several related cytokines, including IL-6, IL-11, IL-27, Leukemia Inhibitory Factor (LIF), Oncostatin M (OSM), Ciliary Neurotrophic Factor (CNTF), Cardiotrophin 1 (CT-1) and Cardiotrophin-like Cytokine (CLC). Gp130 plays critical roles during development and gp...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1189/jlb.0410217

    authors: Silver JS,Hunter CA

    更新日期:2010-12-01 00:00:00

  • Immobilization of rolling NK cells on platelet-borne P-selectin under flow by proinflammatory stimuli, interleukin-12, and leukotriene B4.

    abstract::Recruitment of leukocytes from bloodstream to extrahematic sites is tightly regulated by a variety of adhesion molecules that are expressed on the leukocytes and the vessel walls. In this manuscript, we describe the interactions between natural killer (NK) cells and activated, autologous platelets under physiologic fl...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0204106

    authors: Sheikh S,Parhar RS,Bakheet R,Saleh S,Collison K,Al-Mohanna F

    更新日期:2004-09-01 00:00:00

  • Prostaglandin E2 and thromboxane B2 release from human monocytes treated with bacterial lipopolysaccharide.

    abstract::We investigated the capacity of counterflow-isolated human monocytes to independently synthesize thromboxane B2 (TxB2) and prostaglandin E2 (PGE2) when stimulated with bacterial lipopolysaccharide (LPS). Independent metabolism was confirmed by establishing different specific activities (dpm/ng) of TxB2 and PGE2 releas...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.44.5.376

    authors: Nichols FC,Garrison SW,Davis HW

    更新日期:1988-11-01 00:00:00

  • Cathelicidins, multifunctional peptides of the innate immunity.

    abstract::Cathelicidins comprise a family of mammalian proteins containing a C-terminal cationic antimicrobial domain that becomes active after being freed from the N-terminal cathelin portion of the holoprotein. Many other members of this family have been identified since the first cathelicidin sequences were reported 10 years...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1189/jlb.0403147

    authors: Zanetti M

    更新日期:2004-01-01 00:00:00

  • Characteristics of alveolar macrophages in experimental septic lung.

    abstract::We investigated the pathogenesis of lung injury in sepsis (septic adult respiratory distress syndrome) by focusing on the functional changes of alveolar macrophages (AMs). Sepsis was induced in male WK rats by cecal ligation and puncture. Histological examination of the lungs from this experimental model revealed edem...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.52.2.236

    authors: Goya T,Abe M,Shimura H,Torisu M

    更新日期:1992-08-01 00:00:00

  • Activity and subcellular distribution of cathepsins in primary human monocytes.

    abstract::Cathepsins (Cat) in antigen presenting cells (APC) control antigen processing as well as major histocompatibility complex class II transport and function. The set of active Cat and the subcellular architecture of the class II antigen presentation compartment are largely unknown in primary human APC, including peripher...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0802398

    authors: Greiner A,Lautwein A,Overkleeft HS,Weber E,Driessen C

    更新日期:2003-02-01 00:00:00

  • C1qR(P), a myeloid cell receptor in blood, is predominantly expressed on endothelial cells in human tissue.

    abstract::C1qR(P) is a type I cell surface glycoprotein that has been shown to enhance ingestion of suboptimally opsonized targets by phagocytes in vitro. In this study, we developed and characterized polyclonal antibodies to study the tissue distribution of this receptor targeted to either the N- or C-terminal portion of the m...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:

    authors: Fonseca MI,Carpenter PM,Park M,Palmarini G,Nelson EL,Tenner AJ

    更新日期:2001-11-01 00:00:00

  • Scavenger receptor A mediates H2O2 production and suppression of IL-12 release in murine macrophages.

    abstract::Although class A type I/II scavenger receptor (SR-A) is involved in numerous macrophage functions, its signaling ability remains uncertain. We used monoclonal antibodies (mAb) to specifically stimulate receptors on mouse alveolar (AMs) and peritoneal macrophages (PMs). Immobilized anti-SR-A (2F8) and anti-FcgammaR II/...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0504270

    authors: Józefowski S,Kobzik L

    更新日期:2004-11-01 00:00:00

  • A single assay for measuring the rates of phagocytosis and bacterial killing by neutrophils.

    abstract::We have developed a method that enables the rates of phagocytosis and killing of bacteria by neutrophils to be measured in a single assay. Neutrophils were incubated with bacteria, and at specific intervals were separated from uningested bacteria by low speed centrifugation. Rates of phagocytosis and killing were calc...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.55.2.147

    authors: Hampton MB,Vissers MC,Winterbourn CC

    更新日期:1994-02-01 00:00:00

  • Genetic regulators of myelopoiesis and leukemic signaling identified by gene profiling and linear modeling.

    abstract::Mechanisms controlling the balance between proliferation and self-renewal versus growth suppression and differentiation during normal and leukemic myelopoiesis are not understood. We have used the bi-potent FDB1 myeloid cell line model, which is responsive to myelopoietic cytokines and activated mutants of the granulo...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0206112

    authors: Brown AL,Wilkinson CR,Waterman SR,Kok CH,Salerno DG,Diakiw SM,Reynolds B,Scott HS,Tsykin A,Glonek GF,Goodall GJ,Solomon PJ,Gonda TJ,D'Andrea RJ

    更新日期:2006-08-01 00:00:00

  • Viral dsRNA-activated human dendritic cells produce IL-27, which selectively promotes cytotoxicity in naive CD8+ T cells.

    abstract::Viral recognition programs DCs to express Signal 3 molecules that promote the differentiation of effector CD8(+) T cells. Besides IL-12, another DC-derived IL-12 family member, IL-27, has been reported to contribute herein, but its specific role is not well understood. Here, we show that whereas IL-12 potently induces...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0112045

    authors: de Groot R,van Beelen AJ,Bakdash G,Taanman-Kueter EW,de Jong EC,Kapsenberg ML

    更新日期:2012-09-01 00:00:00

  • Selenium and the immune response: 1. Modulation of alloreactive human lymphocyte functions in vitro.

    abstract::A role for the dietary trace mineral element selenium in the reduction of cancer incidence has been documented in numerous epidemiological and experimental studies. The precise mechanism of this antitumor effect is not well understood, but published data suggest that both inhibition of tumor cell growth and enhancemen...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.45.3.207

    authors: Petrie HT,Klassen LW,Kay HD

    更新日期:1989-03-01 00:00:00

  • Inhibiting Mer receptor tyrosine kinase suppresses STAT1, SOCS1/3, and NF-κB activation and enhances inflammatory responses in lipopolysaccharide-induced acute lung injury.

    abstract::Mer signaling participates in a novel inhibitory pathway in TLR activation. The purpose of the present study was to examine the role of Mer signaling in the down-regulation of TLR4 activation-driven immune responses in mice, i.t.-treated with LPS, using the specific Mer-blocking antibody. At 4 h and 24 h after LPS tre...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0611289

    authors: Lee YJ,Han JY,Byun J,Park HJ,Park EM,Chong YH,Cho MS,Kang JL

    更新日期:2012-06-01 00:00:00

  • RAGE: therapeutic target and biomarker of the inflammatory response--the evidence mounts.

    abstract::The RAGE binds multiple ligand families linked to hyperglycemia, aging, inflammation, neurodegeneration, and cancer. Activation of RAGE by its ligands stimulates diverse signaling cascades. The recent observation that the cytoplasmic domain of RAGE interacts with diaphanous or mDia-1 links RAGE signal transduction to ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1189/jlb.0409230

    authors: Ramasamy R,Yan SF,Schmidt AM

    更新日期:2009-09-01 00:00:00

  • Respiratory viral infection, epithelial cytokines, and innate lymphoid cells in asthma exacerbations.

    abstract::Exacerbations of asthma are most commonly triggered by viral infections, which amplify allergic inflammation. Cytokines released by virus-infected AECs may be important in driving this response. This review focuses on accumulating evidence in support of a role for epithelial cytokines, including IL-33, IL-25, and TSLP...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章,评审

    doi:10.1189/jlb.3RI0314-129R

    authors: Kumar RK,Foster PS,Rosenberg HF

    更新日期:2014-09-01 00:00:00

  • Modulation of IL-1 alpha, IL-1 beta, and 25K Mr non-IL-1 activity released by human mononuclear cells.

    abstract::To determine if the release of IL-1 alpha and IL-1 beta by cultured PBMC could be independently modulated by different exogenous stimuli, we examined the effect of LPS, IFN gamma, latex beads, and indomethacin on the release of IL-1 alpha and IL-1 beta. PBMC culture supernatants were fractionated by Sephacryl-S-200 co...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.46.5.417

    authors: Sandborg CI,Berman MA,Imfeld KL,Zaldivar F Jr,Masada MP,Kenney JS

    更新日期:1989-11-01 00:00:00

  • Relative role of chloramines, hypochlorous acid, and proteases in the activation of human polymorphonuclear leukocyte collagenase.

    abstract::The activation of collagenase released by polymorphonuclear leukocytes (PMNs) has been extensively studied in vitro, but the activation of the enzyme in vivo is not fully understood. For further evaluation of the relative role of oxidative and proteolytic mechanisms in the activation of collagenase, PMNs were stimulat...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.60.5.598

    authors: Claesson R,Karlsson M,Zhang Y,Carlsson J

    更新日期:1996-11-01 00:00:00

  • Alpha lipoic acid promotes development of hematopoietic progenitors derived from human embryonic stem cells by antagonizing ROS signals.

    abstract::Antagonism of ROS signaling can inhibit cell apoptosis and autophagy, thus favoring the maintenance and expansion of hematopoietic stem cells. Alpha lipoic acid (ALA), a small antioxidant molecule, affects cell apoptosis by lowering the ROS level. In this study, we show that ALA promoted production of human pluripoten...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/JLB.1A0520-179R

    authors: Dong Y,Bai J,Zhang Y,Zhou Y,Pan X,Li X,Zhou Q,Chen Y,Lai M,Mao B,Bian G,Feng J,Xie F,Chen B,Nakahata T,Zhang Y,Ma F

    更新日期:2020-12-01 00:00:00

  • The alpha4beta1 integrin can mediate leukocyte adhesion to casein and denatured protein substrates.

    abstract::An understanding of the binding specificity of leukocyte integrins is important to determine the range of ligands that interact with these receptors during inflammatory processes. In this study we show that the alpha4beta1 integrin can interact with casein and denatured albumin and promote leukocyte adhesion through t...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.62.3.318

    authors: Davis GE,Thomas JS,Madden S

    更新日期:1997-09-01 00:00:00

  • Antibody ligation of murine Ly-6G induces neutropenia, blood flow cessation, and death via complement-dependent and independent mechanisms.

    abstract::Ly-6G is a member of the Ly-6 family of GPI-linked proteins, which is expressed on murine neutrophils. Antibodies against Ly-6G cause neutropenia, and fatal reactions also develop if mice are primed with TNF-alpha prior to antibody treatment. We have investigated the mechanisms behind these responses to Ly-6G ligation...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.0507305

    authors: Abbitt KB,Cotter MJ,Ridger VC,Crossman DC,Hellewell PG,Norman KE

    更新日期:2009-01-01 00:00:00

  • An essential role for IFN-β in the induction of IFN-stimulated gene expression by LPS in macrophages.

    abstract::TLR agonists such as LPS and poly(I:C) induce expression of type I IFNs, such as IFN-α and -β, by macrophages. To examine the role of IFN-β in the induction of ISGs by LPS, we compared the ability of LPS to induce ISGF3 activity and ISG expression in bone marrow-derived macrophages from WT and Ifnb1(-/-) mice. We foun...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1189/jlb.2A0414-191R

    authors: Sheikh F,Dickensheets H,Gamero AM,Vogel SN,Donnelly RP

    更新日期:2014-10-01 00:00:00

  • Inhibitory actions of glucosamine, a therapeutic agent for osteoarthritis, on the functions of neutrophils.

    abstract::Glucosamine, an amino monosaccharide naturally occurring in the connective and cartilage tissues, contributes to maintaining the strength, flexibility, and elasticity of these tissues. In recent years, glucosamine has been used widely to treat osteoarthritis in humans and animal models. Neutrophils, which usually func...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:

    authors: Hua J,Sakamoto K,Nagaoka I

    更新日期:2002-04-01 00:00:00

  • Reperfusion-induced oxidative stress in diabetes: cellular and enzymatic sources.

    abstract::Reactive oxygen metabolites (ROMs) have been implicated in the pathogenesis of the inflammatory response to ischemia/reperfusion (I/R), which is exacerbated in diabetes. This study revealed an increased (P < 0.01) ROMs production in mesenteric tissue (measured using the oxidant-sensitive fluorochrome dihydrorhodamine ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.66.1.59

    authors: Salas A,Panés J,Elizalde JI,Granger DN,Piqué JM

    更新日期:1999-07-01 00:00:00

  • Polymorphonuclear granulocytes induce myocardial dysfunction during ischemia and in later reperfusion of hearts exposed to low-flow ischemia.

    abstract::Polymorphonuclear granulocytes (PMNs) are known to contribute to reperfusion injury of the heart. However, whether PMNs compromise myocardial function of hearts exposed to a low-flow ischemia has not been determined. Moreover, not much is known about deleterious effects of PMNs at different times during ischemia and r...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:

    authors: Seligmann C,Bock A,Leitsch T,Schimmer M,Simsek Y,Schultheiss HP

    更新日期:2001-05-01 00:00:00

  • Regulation of T cell-dependent and -independent IL-12 production by the three Th2-type cytokines IL-10, IL-6, and IL-4.

    abstract::The production of IL-12 by macrophages/dendritic cells (Mphi/DC) is mediated either by a T cell-dependent pathway that is induced primarily by the interaction of CD40 ligand (CD40L) on activated T cells with CD40 on IL-12-producing cells or by a T cell-independent pathway that is induced by bacteria or bacterial produ...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.61.1.80

    authors: Takenaka H,Maruo S,Yamamoto N,Wysocka M,Ono S,Kobayashi M,Yagita H,Okumura K,Hamaoka T,Trinchieri G,Fujiwara H

    更新日期:1997-01-01 00:00:00

  • Leukosialin (CD43) behavior during adhesion of human monocytic THP-1 cells to red blood cells.

    abstract::To understand the modulation and the behavior of glycocalyx elements during adhesion, we explored one of its components, the CD43 molecule, on human monocytic THP-1 cells exposed to cytokine stimulation and its redistribution during heterotypic adhesion to opsonized erythrocytes. First we demonstrated by immunofluores...

    journal_title:Journal of leukocyte biology

    pub_type: 杂志文章

    doi:10.1002/jlb.61.5.609

    authors: Soler M,Merant C,Servant C,Fraterno M,Allasia C,Lissitzky JC,Bongrand P,Foa C

    更新日期:1997-05-01 00:00:00