Abstract:
BACKGROUND:The functional role of the intracellular diacylglycerol/protein kinase C second-messenger pathway in the regulation of gastric acid secretion and the effects on the involved inositotrisphosphate/Ca2+/calmodulin system are not well understood, and contradictory data have been reported. We therefore evaluated the effects of phorbol ester treatment (tetradecanoylphorbol-12,13-acetate (TPA)) on dibutyryl cyclic adenosine-5' monophosphate (dBcAMP)- and carbachol-stimulated aminopyrine (AP) accumulation in comparison with intracellular alterations of the phospholipase C/inostol phosphate signal transduction pathway in isolated rat gastric parietal cells. METHODS:[14C]AP accumulation was determined as an indirect measure of gastric acid secretion. Inositolphosphate second-messenger activation was investigated with [3H]inositolmonophosphate release in [3H]-myoinositol prelabeled rat gastric parietal cells. RESULTS:TPA at a low concentration of 5 nM caused a small (45%) but significant increase in carbachol (0.1 mM)-stimulated AP accumulation, which was dose-dependently inhibited by higher concentrations of TPA with corresponding shifts in the dose-response curve for carbachol-stimulated AP accumulation. AP uptake stimulated by dBcAMP (0.1 mM) and the synergistic stimulatory effect induced by carbachol together with dBcAMP were inhibited by TPA at all concentrations investigated. In the presence of increasing concentrations of the calcium ionophore ionomycin (10(-8)-10(-5) M) TPA at 5 nM increased AP accumulation (AP ratio was 4.02 with 5 nM TPA versus 1.23 in the absence of TPA; P < 0.05), indicating that phorbol ester stimulates AP uptake in rat parietal cells. Simultaneous investigation of [14C]AP accumulation and [3H]inositol monophosphate release showed that inhibitory effects of TPA on carbachol- and carbachol plus dBcAMP-stimulated cells are mediated by an inhibition of the receptor/G-protein/phospholipase C interaction, leading to a reduction of inositolphosphate release. The costimulation of rat parietal cells with dBcAMP, ionomycin, and TPA (5 nM) did not reproduce the synergistic effects of carbachol together with dBcAMP on AP accumulation, suggesting that carbachol-stimulated AP uptake seems to be additionally mediated by a still unknown pathway independent of intracellular calcium release or protein kinase C activation.
journal_name
Scand J Gastroenteroljournal_title
Scandinavian journal of gastroenterologyauthors
Kopp R,Pfeiffer Adoi
10.1080/003655200750023336subject
Has Abstractpub_date
2000-07-01 00:00:00pages
686-93issue
7eissn
0036-5521issn
1502-7708journal_volume
35pub_type
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journal_title:Scandinavian journal of gastroenterology
pub_type: 杂志文章
doi:10.3109/00365528509089272
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abstract::The occurrence of autoantibodies against intrinsic factor, H,K-ATPase, and pepsinogen was analysed by means of enzyme-linked immunosorbent assay in three groups of sera. Group 1 comprised sera from 14 rheumatoid arthritis patients with normal acid secretion; group 2, sera from 18 rheumatoid arthritis patients with red...
journal_title:Scandinavian journal of gastroenterology
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doi:10.3109/00365529109003961
更新日期:1991-10-01 00:00:00
abstract:OBJECTIVE:Despite the apparent lack of clinical interest in the inlet patches of heterotopic gastric mucosa (HGM) in the upper esophagus, the literature contains reports of complications associated with HGM. The aim of this study was to determine the prevalence and clinical importance of HGM in patients referred for up...
journal_title:Scandinavian journal of gastroenterology
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更新日期:2006-12-01 00:00:00
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journal_title:Scandinavian journal of gastroenterology
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doi:10.3109/00365529109043636
更新日期:1991-06-01 00:00:00
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journal_title:Scandinavian journal of gastroenterology
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doi:10.3109/00365528309182091
更新日期:1983-09-01 00:00:00
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journal_title:Scandinavian journal of gastroenterology
pub_type: 杂志文章
doi:10.1080/00365521.2019.1649456
更新日期:2019-08-01 00:00:00
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journal_title:Scandinavian journal of gastroenterology
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
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abstract::Basal, meal-stimulated, and insulin-stimulated secretion of pancreatic polypeptide (PP) has been measured before and 3 and 12 months after gastric bypass surgery in 10 obese patients. The basal serum concentration of PP was significantly reduced after the operation. After a meal there was a significant increase in PP ...
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doi:10.3109/00365528109181020
更新日期:1981-01-01 00:00:00
abstract::Bile duct hamartomas, also known as von Meyenburg complexes, are benign liver malformations which usually present as multiple small nodules scattered in both lobes of the liver. We report a unique case of bile duct hamartoma. An asymptomatic 30-year-old man who had a solitary cystic lesion underwent partial hepatectom...
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更新日期:2005-11-01 00:00:00
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更新日期:2009-01-01 00:00:00
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doi:10.3109/00365521.2012.660544
更新日期:2012-09-01 00:00:00
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journal_title:Scandinavian journal of gastroenterology
pub_type: 杂志文章
doi:10.3109/00365528609034626
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND:It has been suggested that psychopathology in irritable bowel syndrome (IBS) patients is a function of patient status rather than of the disease. Although there are many studies comparing IBS patients, IBS non-patients, and controls with each other, no previous study has recruited all three groups from a rep...
journal_title:Scandinavian journal of gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2003-05-01 00:00:00
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journal_title:Scandinavian journal of gastroenterology
pub_type: 临床试验,杂志文章,随机对照试验
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pub_type: 杂志文章,评审
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更新日期:2017-11-01 00:00:00
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journal_title:Scandinavian journal of gastroenterology
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doi:10.1080/00365529850172476
更新日期:1998-11-01 00:00:00
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journal_title:Scandinavian journal of gastroenterology
pub_type: 杂志文章
doi:10.1080/00365520802029856
更新日期:2008-01-01 00:00:00
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journal_title:Scandinavian journal of gastroenterology
pub_type: 杂志文章
doi:10.1080/00365520410003344
更新日期:2004-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-06-01 00:00:00
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journal_title:Scandinavian journal of gastroenterology
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doi:10.3109/00365528509088842
更新日期:1985-09-01 00:00:00
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journal_title:Scandinavian journal of gastroenterology
pub_type: 杂志文章
doi:10.3109/00365529709011224
更新日期:1997-10-01 00:00:00
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doi:10.3109/00365529709011191
更新日期:1997-09-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2010-09-01 00:00:00