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Detection of Helicobacter pylori gene by means of immunomagnetic separation-based polymerase chain reaction in feces.

Abstract：

BACKGROUND:Detection of Helicobacter pylori is usually performed by culture, polymerase chain reaction (PCR), histology, or urease test on gastric biopsy samples. Although methods based on feces are non-invasive, their sensitivity has been relatively low. In this study, to improve its sensitivity, immunomagnetic separation (IMS) was used as a pre-PCR step for direct detection of H. pylori in feces. METHODS:Fresh fecal samples were taken from 72 patients attending for endoscopy. Of these, 57 patients had a positive H. pylori status according to the results of culture, histology, and PCR on gastric biopsy samples. Anti-H. pylori antibody-sensitized immunomagnetic beads were used to concentrate the bacteria. PCR was then performed to detect the H. pylori urease A-encoding gene. RESULTS:Of the 57 H. pylori-positive patients, 35 (61.4%) had positive fecal samples by IMS-based PCR method. None of the 15 H. pylori-negative patients had positive fecal samples. The sensitivity of this method was 61.4%, and the specificity 100.0%. CONCLUSIONS:This study confirms that non-invasive diagnosis of H. pylori infection could be made from feces by using IMS-based PCR.

journal_name

Scand J Gastroenterol

journal_title

Scandinavian journal of gastroenterology

authors

Watanabe T,Tomita S,Kudo M,Kurokawa M,Orino A,Todo A,Chiba T

doi

10.1080/00365529850172476

subject

Has Abstract

pub_date

1998-11-01 00:00:00

pages

1140-3

issue

11

eissn

0036-5521

issn

1502-7708

journal_volume

33

pub_type

杂志文章

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